LOCUS       NM_001107497            3568 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus SPT5 homolog, DSIF elongation factor subunit
            (Supt5h), mRNA.
ACCESSION   NM_001107497 XM_001078198 XM_218382
VERSION     NM_001107497.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Kwak H and Lis JT.
  TITLE     Control of transcriptional elongation
  JOURNAL   Annu Rev Genet 47, 483-508 (2013)
   PUBMED   24050178
  REMARK    Review article
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y,
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E,
            Bonneau R, Selbach M, Dieterich C and Landthaler M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   3  (bases 1 to 3568)
  AUTHORS   Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein
            C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J and
            Hentze MW.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   4  (bases 1 to 3568)
  AUTHORS   McKnight NC, Jefferies HB, Alemu EA, Saunders RE, Howell M,
            Johansen T and Tooze SA.
  TITLE     Genome-wide siRNA screen reveals amino acid starvation-induced
            autophagy requires SCOC and WAC
  JOURNAL   EMBO J 31 (8), 1931-1946 (2012)
   PUBMED   22354037
REFERENCE   5  (bases 1 to 3568)
  AUTHORS   Schneider DA.
  TITLE     RNA polymerase I activity is regulated at multiple steps in the
            transcription cycle: recent insights into factors that influence
            transcription elongation
  JOURNAL   Gene 493 (2), 176-184 (2012)
   PUBMED   21893173
  REMARK    Review article
REFERENCE   6  (bases 1 to 3568)
  AUTHORS   Lau NC, Seto AG, Kim J, Kuramochi-Miyagawa S, Nakano T, Bartel DP
            and Kingston RE.
  TITLE     Characterization of the piRNA complex from rat testes
  JOURNAL   Science 313 (5785), 363-367 (2006)
   PUBMED   16778019
REFERENCE   7  (bases 1 to 3568)
  AUTHORS   Cros N, Tkatchenko AV, Pisani DF, Leclerc L, Leger JJ, Marini JF
            and Dechesne CA.
  TITLE     Analysis of altered gene expression in rat soleus muscle atrophied
            by disuse
  JOURNAL   J Cell Biochem 83 (3), 508-519 (2001)
   PUBMED   11596118
REFERENCE   8  (bases 1 to 3568)
  AUTHORS   Yamaguchi Y, Wada T, Watanabe D, Takagi T, Hasegawa J and Handa H.
  TITLE     Structure and function of the human transcription elongation factor
            DSIF
  JOURNAL   J Biol Chem 274 (12), 8085-8092 (1999)
   PUBMED   10075709
REFERENCE   9  (bases 1 to 3568)
  AUTHORS   Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S,
            Sugimoto S, Yano K, Hartzog GA, Winston F, Buratowski S and Handa
            H.
  TITLE     DSIF, a novel transcription elongation factor that regulates RNA
            polymerase II processivity, is composed of human Spt4 and Spt5
            homologs
  JOURNAL   Genes Dev 12 (3), 343-356 (1998)
   PUBMED   9450929
REFERENCE   10 (bases 1 to 3568)
  AUTHORS   Asakura K, Pogulis RJ, Pease LR and Rodriguez M.
  TITLE     A monoclonal autoantibody which promotes central nervous system
            remyelination is highly polyreactive to multiple known and novel
            antigens
  JOURNAL   J Neuroimmunol 65 (1), 11-19 (1996)
   PUBMED   8642059
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB751273.1, BC166741.1, CR755056.1 and DY319730.1.
            
            On Sep 4, 2012 this sequence version replaced NM_001107497.1.
            
            Sequence Note: This RefSeq record was created from transcript data
            from different strains because no single transcript from the same
            strain was available for the full length of the gene. The extent of
            this transcript is supported by transcript alignments and
            orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-320               CB751273.1         59-378
            321-387             BC166741.1         285-351
            388-812             CR755056.1         58-482              c
            813-1904            BC166741.1         1726-2817
            1905-2525           DY319730.1         127-747
            2526-3568           BC166741.1         3404-4446
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     gene            1..3568
                     /gene="Supt5h"
                     /note="SPT5 homolog, DSIF elongation factor subunit"
                     /db_xref="GeneID:308472"
                     /db_xref="RGD:1563807"
     exon            1..74
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            75..158
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..3335
                     /gene="Supt5h"
                     /note="suppressor of Ty 5 homolog"
                     /codon_start=1
                     /product="transcription elongation factor SPT5"
                     /protein_id="NP_001100967.2"
                     /db_xref="GeneID:308472"
                     /db_xref="RGD:1563807"
                     /translation="MSDSEDSNFSEEEDSERSSEAEEAEVEEDRRSAAGSEKEEEPEE
                     EEEEEEEEYDEEEEEEDDDRPPKKPRHGGFILDEADVDDEYEDEDQWEDGAEDILEKE
                     EIEASNIDNVVLDEDRSGARRLQNLWRDQREEELGEYYMKKYAKSSVGETVYGGSDEL
                     SDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFIAYQFTDTPLQIKSVVAPE
                     HVKGYIYVEAYKQTHVKQAIEGVGNLRLGYWNQQMVPIKEMTDVLKVVKEVANLKPKS
                     WVRLKRGIYKDDIAQVDYVEPSQNTISLKMIPRIDYDRIKARMSLKDWFAKRKKFKRP
                     PQRLFDAEKIRSLGGDVASDGDFLIFEGNRYSRKGFLFKSFAMSAVITEGVKPTLSEL
                     EKFEDQPEGIDLEVVTESTGKEREHNFQPGDNVEVCEGELINLQGKVLSVDGNKITIM
                     PKHEDLKDMLEFPAQELRKYFKMGDHVKVIAGRFEGDTGLIVRVEENFVILFSDLTMH
                     ELKVLPRDLQLCSETASGVDVGGQHEWGELVQLDPRTVGVIVRLERETFQVLNMHGKV
                     VTVRHQAVTQKKDNRFAVALDSDQNNIHVKDIVKVIDGPHSGREGEIRHLYRSFAFLH
                     CKKLVENGGMFVCKARHLVLAGGSKPRDVTNLTVGGFTPMSPRISSPMHPSAEGQHGG
                     FGSPGGMSRGRGRRDNELIGQTVRISQGPYKGYIGVVKDATESTARVELHSTCQTISV
                     DRQRLTTVDSQRPGGMTSTYGRTPMYGSQTPMYGSGSRTPMYGSQTPLQDGSRTPHYG
                     SQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAFDDEPTPSPQAYGGTPNPQTPG
                     YPDPSSPQVNPQYNPQTPGTPAMYNTDQFSPYAAPSPQGSYQPSPSPQSYHQVAPSPA
                     GYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSGIEQNSS
                     DWVTTDIQVKVRDTYLDTQIVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITP
                     TKNNKVKVILGEDREATGVLLSIDGEDGIIRMDLEDQQIKILNLRFLGKLLEA"
     exon            159..321
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            322..387
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            388..399
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            400..469
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            470..538
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            539..604
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            605..635
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            636..704
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            705..956
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1046
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1117
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1223
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1317
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1454
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1610
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1757
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1904
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2030
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            2031..2115
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2223
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..2326
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            2327..2454
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            2455..2560
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2719
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2888
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            2889..3092
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            3093..3188
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
     exon            3189..3554
                     /gene="Supt5h"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgagagagcg ctcaggccag ctaggcgttg tgtgatcagc agctggcgcg gaagcttcag
       61 gtggacagtg atcgttgcgg aagatgtcgg acagcgaaga cagcaacttc tcggaggagg
      121 aggacagcga gcgcagcagc gaggcggagg aggccgaggt agaggaagac cggcgaagtg
      181 ccgcaggcag tgaaaaagaa gaggagcccg aggaagagga ggaggaggaa gaggaggaat
      241 acgatgagga ggaggaggag gaggatgatg accggcctcc caagaaacca cgccatgggg
      301 gcttcatttt agatgaggct gatgtcgatg atgaatatga agatgaagac cagtgggaag
      361 atggagctga agatatcctg gagaaagaag agattgaagc ctccaacatt gataacgttg
      421 tgctggatga agaccgctct ggggctcgtc gtctgcaaaa tctctggagg gaccagagag
      481 aagaagaact gggcgagtat tacatgaaga aatatgccaa gtcatctgtg ggagagacgg
      541 tatatggagg gtctgatgag ctctcagatg acatcaccca gcagcagctg ctcccaggag
      601 tcaaggaccc caacctgtgg acggtcaagt gtaagattgg ggaggaacgg gccacagcaa
      661 tttccttgat gcgaaaattc atcgcttacc agttcacaga cacacccctg cagattaagt
      721 cagtggttgc cccggagcat gtgaagggct acatctatgt ggaggcctac aaacagaccc
      781 atgtgaagca ggccattgag ggtgtgggca acctgcggct tggctactgg aaccagcaga
      841 tggtgcctat taaggagatg accgatgtac tcaaggtggt gaaggaggtg gccaacctga
      901 agccgaagtc ctgggtccgc ctcaagagag gcatctacaa agatgacatc gctcaggtgg
      961 actacgtcga gcccagccag aacaccattt ccctgaagat gatcccgcgc attgactatg
     1021 accgcatcaa agcccgcatg agcttgaaag actggtttgc caaaaggaag aagttcaagc
     1081 ggcctccaca gaggctcttt gatgctgaaa agatcaggtc cttggggggt gatgttgcct
     1141 ctgatggtga cttcctcatc tttgagggta accgctacag ccggaagggc ttcctgttca
     1201 agagttttgc catgtctgct gtgattacag aaggtgtaaa gcccaccctg tcagagctgg
     1261 aaaagtttga ggatcagcct gagggcatcg acttggaggt ggtgactgaa agcacaggga
     1321 aggagcgaga gcacaatttc cagcctggtg acaatgtgga ggtgtgtgag ggtgagctca
     1381 tcaacctgca gggcaaggtc ctcagcgtgg atggcaacaa gatcaccatc atgcccaaac
     1441 atgaggacct caaggacatg cttgagttcc cagctcagga acttcggaag tacttcaaga
     1501 tgggagacca tgtaaaagtg attgctggcc gatttgaggg tgacacaggt ctcatcgtgc
     1561 gggtggaaga gaactttgtt attcttttct cagacctcac catgcacgag ttgaaagtgc
     1621 ttccccggga tctgcagctc tgctcagaga cagcctcagg tgtggatgtt gggggccagc
     1681 atgagtgggg tgagctggtg cagttggatc cccggactgt aggtgtcatc gtgcggctgg
     1741 agcgagagac cttccaggtt ctcaacatgc atgggaaagt ggtgactgta aggcaccagg
     1801 ctgtgacgca gaaaaaggac aaccgcttcg ctgtggctct ggactcagat cagaacaaca
     1861 tccatgtgaa ggacattgtt aaggtcattg atggccccca ttcaggccga gagggtgaga
     1921 tccgccatct gtaccgcagc tttgccttcc tgcattgcaa gaagctggtg gagaatggcg
     1981 gcatgtttgt ctgtaaggca cggcacctgg tgttggctgg gggctcaaag ccccgggatg
     2041 tgaccaattt aactgttggt ggcttcactc ctatgagccc ccggatcagc agccctatgc
     2101 accccagtgc tgaaggtcag catggtggtt ttggaagccc aggtggcatg agcaggggtc
     2161 gagggcgaag agacaatgag ctcataggcc agactgtgcg tatctcccag ggaccctaca
     2221 aaggctacat tggtgtggtg aaggatgcca cggagtccac agcccgagta gaattgcact
     2281 ctacctgcca gaccatctct gtggaccgcc agcggctcac cacagttgac tcccagcgtc
     2341 caggtggcat gacttccacc tatggaagga ctcccatgta tggctctcag actcccatgt
     2401 atggctctgg ctcccgaaca cccatgtatg gctcccagac acctctgcag gatggtagcc
     2461 gcactccaca ttatggctca cagacacccc tgcatgatgg tagccgcact cctgctcaga
     2521 gtggggcctg ggatcccaac aaccctaaca caccttccag ggctgaggag gaatacgaat
     2581 atgcctttga cgacgaaccc accccatccc cacaagcata tgggggaacc ccgaatcccc
     2641 aaacacccgg ctacccagac ccatcatccc cccaggtcaa tccacagtac aacccgcaga
     2701 cacccgggac gccagccatg tacaacacag accagttctc cccctacgct gccccctccc
     2761 cacaggggtc ttaccagccc agccccagcc cccagagcta ccaccaagtg gcaccaagtc
     2821 cagcgggcta tcagaacacc cattccccag ccagctacca cccaacaccg tcacccatgg
     2881 cctatcaggc tagtcccagc cccagccctg ttggctacag tccaatgacc cctggagctc
     2941 cttctcctgg aggctacaat ccccacacac caggctcagg cattgagcag aactccagtg
     3001 actgggtgac cactgatatc caggtgaagg ttcgagacac ctatctggac acacaaatag
     3061 tggggcagac aggcgtcatc cgcagtgtca cgggaggcat gtgctccgtg tacctgaaag
     3121 atagtgagaa ggtggtcagt atctccagtg agcatctgga gcccatcacc cccaccaaga
     3181 acaacaaggt gaaagtgatt ctgggtgagg accgagaggc taccggggtc cttctgagta
     3241 ttgatggtga ggatggcatc atccgaatgg acctggaaga ccagcagatc aagattctaa
     3301 acctccgctt cctagggaag ctcctggagg cctgaggccc aagcgtgcgg agcaagcagc
     3361 ccgctctggc cttgcttggc ctaggatgga accccgagtc tggggcaagc cagggcaatc
     3421 actctggatt tccttttcct cctcccctgg tactctagga atcagagcag tctgaggaat
     3481 gtcctgcctt ctttctgctg tcccagcttg cctttgtgtt caggcctttc aataaagaga
     3541 aactgtttga aaaaaaaaaa aaaaaaaa
//
