LOCUS       NM_053752               1273 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus succinate-CoA ligase GDP/ADP-forming subunit
            alpha (Suclg1), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_053752
VERSION     NM_053752.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Kibbey,R.G., Pongratz,R.L., Romanelli,A.J., Wollheim,C.B.,
            Cline,G.W. and Shulman,G.I.
  TITLE     Mitochondrial GTP regulates glucose-stimulated insulin secretion
  JOURNAL   Cell Metab 5 (4), 253-264 (2007)
   PUBMED   17403370
  REMARK    GeneRIF: In INS-1 832/13 cells and cultured rat islets, siRNA
            suppression of the GTP-producing pathway reduced glucose-stimulated
            insulin secretion by 50%.
REFERENCE   3  (bases 1 to 1273)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   4  (bases 1 to 1273)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   5  (bases 1 to 1273)
  AUTHORS   Kowluru,A., Tannous,M. and Chen,H.Q.
  TITLE     Localization and characterization of the mitochondrial isoform of
            the nucleoside diphosphate kinase in the pancreatic beta cell:
            evidence for its complexation with mitochondrial succinyl-CoA
            synthetase
  JOURNAL   Arch Biochem Biophys 398 (2), 160-169 (2002)
   PUBMED   11831846
REFERENCE   6  (bases 1 to 1273)
  AUTHORS   Adams,L.D., Lemire,J.M. and Schwartz,S.M.
  TITLE     A systematic analysis of 40 random genes in cultured vascular
            smooth muscle subtypes reveals a heterogeneity of gene expression
            and identifies the tight junction gene zonula occludens 2 as a
            marker of epithelioid 'pup' smooth muscle cells and a participant
            in carotid neointimal formation
  JOURNAL   Arterioscler Thromb Vasc Biol 19 (11), 2600-2608 (1999)
   PUBMED   10559001
REFERENCE   7  (bases 1 to 1273)
  AUTHORS   Henning,W.D., Upton,C., McFadden,G., Majumdar,R. and Bridger,W.A.
  TITLE     Cloning and sequencing of the cytoplasmic precursor to the alpha
            subunit of rat liver mitochondrial succinyl-CoA synthetase
  JOURNAL   Proc Natl Acad Sci U S A 85 (5), 1432-1436 (1988)
   PUBMED   3422742
REFERENCE   8  (bases 1 to 1273)
  AUTHORS   Ball,D.J. and Nishimura,J.S.
  TITLE     Affinity chromatography and affinity labeling of rat liver
            succinyl-CoA synthetase
  JOURNAL   J Biol Chem 255 (22), 10805-10812 (1980)
   PUBMED   7430155
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC061537.1.
            
            On Apr 4, 2007 this sequence version replaced NM_053752.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061537.1, FQ212668.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1273              BC061537.1         5-1277
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q32"
     gene            1..1273
                     /gene="Suclg1"
                     /note="succinate-CoA ligase GDP/ADP-forming subunit alpha"
                     /db_xref="GeneID:114597"
                     /db_xref="RGD:619821"
     exon            1..109
                     /gene="Suclg1"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..1053
                     /gene="Suclg1"
                     /EC_number="6.2.1.4"
                     /EC_number="6.2.1.5"
                     /note="succinate-CoA ligase, GDP-forming alpha subunit;
                     succinyl-CoA ligase [GDP-forming] subunit alpha,
                     mitochondrial; succinyl-CoA synthetase alpha subunit;
                     succinate-CoA ligase, alpha subunit; SCS-alpha;
                     succinyl-CoA synthetase subunit alpha; succinyl-CoA ligase
                     [ADP/GDP-forming] subunit alpha, mitochondrial;
                     succinate--CoA ligase [ADP/GDP-forming] subunit alpha,
                     mitochondrial"
                     /codon_start=1
                     /product="succinate--CoA ligase [ADP/GDP-forming] subunit
                     alpha, mitochondrial precursor"
                     /protein_id="NP_446204.2"
                     /db_xref="GeneID:114597"
                     /db_xref="RGD:619821"
                     /translation="MTAAVVAAAATATMVSGSSGLAAARLLSRTFLLQQNGIRHGSYT
                     ASRKNIYIDKNTKVICQGFTGKQGTFHSQQALEYGTKLVGGTTPGKGGKKHLGLPVFN
                     TVKEAKEKTGATASVIYVPPPFAAAAINEAIDAEIPLVVCITEGIPQQDMVRVKHKLT
                     RQGKTRLIGPNCPGIINPGECKIGIMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGL
                     GQSLCIGIGGDPFNGTNFIDCLDVFLKDPATEGIVLIGEIGGHAEENAAEFLKEHNSG
                     PKAKPVVSFIAGITAPPGRRMGHAGAIIAGGKGGAKEKISALQSAGVIVSMSPAQLGT
                     CMYKEFEKRKML"
     exon            110..213
                     /gene="Suclg1"
                     /inference="alignment:Splign:2.1.0"
     exon            214..330
                     /gene="Suclg1"
                     /inference="alignment:Splign:2.1.0"
     exon            331..543
                     /gene="Suclg1"
                     /inference="alignment:Splign:2.1.0"
     exon            544..601
                     /gene="Suclg1"
                     /inference="alignment:Splign:2.1.0"
     exon            602..685
                     /gene="Suclg1"
                     /inference="alignment:Splign:2.1.0"
     exon            686..837
                     /gene="Suclg1"
                     /inference="alignment:Splign:2.1.0"
     exon            838..1026
                     /gene="Suclg1"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1242
                     /gene="Suclg1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcattgggg gaatgaccgc agcggtagtt gctgcggctg ccactgcaac catggtctcc
       61 ggcagcagcg gccttgccgc cgcccgcctg ctgtcgcgca ccttcctcct gcaacagaat
      121 ggaatacggc acgggtctta cacagcctct cggaaaaata tctatattga taaaaatacg
      181 aaggttattt gccagggttt cacaggcaag cagggtacct ttcacagcca gcaggctttg
      241 gagtacggca ccaaactcgt gggaggaacc actccaggga aagggggcaa gaagcacctg
      301 ggcttacctg tctttaatac tgtgaaggaa gccaaagaga aaacaggagc gacagcttct
      361 gtcatttatg tccctcctcc tttcgctgct gccgccatca atgaagcaat tgacgcagag
      421 attcccttgg ttgtgtgcat tactgaaggt attccgcagc aggacatggt gcgggtcaag
      481 cacaagctga cacgccaggg gaagacgagg ctaattgggc caaactgccc cggtatcatc
      541 aaccctggag aatgcaaaat cggcatcatg cctggccaca ttcacaagaa aggaagaata
      601 ggtatcgtgt ccaggtccgg tactctgact tatgaagcag ttcaccaaac aacccaagtt
      661 ggattggggc agtccttgtg tattggcatt ggaggtgacc cttttaatgg gactaatttt
      721 attgattgcc ttgacgtctt cctgaaggat ccagctacag aaggcatcgt actgattggt
      781 gaaattggtg gtcacgctga agaaaatgct gcggagtttc tgaaggagca taactcaggt
      841 ccaaaggcca agcctgtagt gtccttcatt gctggtataa ctgctcctcc tggcagaagg
      901 atgggccacg caggggcgat tattgctgga ggaaaaggtg gcgccaaaga gaagatctct
      961 gctcttcaga gtgcgggtgt aattgtcagc atgtcccccg cacagctggg aacctgcatg
     1021 tataaggaat ttgaaaagag gaagatgctt tgaaggacac gacatgaatt ttaaggctgt
     1081 ggaggaaatc tcacagactg gagagcccgt ccagactgca gcccaggcac ctgaccctgg
     1141 tctctcagta caggggaagt ccaggcacgt tttagaatgt cctaaattga gctattttca
     1201 cttactgtgt aacagagaca gataataaat ctatcatttg ataaaaaaaa aaaaaaaaaa
     1261 aaaaaaaaaa aaa
//
