LOCUS       NM_178345               7172 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus syntaxin binding protein 5 (Stxbp5), transcript
            variant b, mRNA.
ACCESSION   NM_178345
VERSION     NM_178345.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Saldate,J.J., Shiau,J., Cazares,V.A. and Stuenkel,E.L.
  TITLE     The ubiquitin-proteasome system functionally links neuronal
            Tomosyn-1 to dendritic morphology
  JOURNAL   J Biol Chem 293 (7), 2232-2246 (2018)
   PUBMED   29269412
  REMARK    GeneRIF: Tomo-1 overexpression increased dendritic spine density,
            whereas Tomo-1 knockdown (KD) decreased spine density.
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Geerts,C.J., Mancini,R., Chen,N., Koopmans,F.T.W., Li,K.W.,
            Smit,A.B., van Weering,J.R.T., Verhage,M. and Groffen,A.J.A.
  TITLE     Tomosyn associates with secretory vesicles in neurons through its
            N- and C-terminal domains
  JOURNAL   PLoS One 12 (7), e0180912 (2017)
   PUBMED   28746398
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7172)
  AUTHORS   Cazares,V.A., Njus,M.M., Manly,A., Saldate,J.J., Subramani,A.,
            Ben-Simon,Y., Sutton,M.A., Ashery,U. and Stuenkel,E.L.
  TITLE     Dynamic Partitioning of Synaptic Vesicle Pools by the SNARE-Binding
            Protein Tomosyn
  JOURNAL   J Neurosci 36 (44), 11208-11222 (2016)
   PUBMED   27807164
REFERENCE   4  (bases 1 to 7172)
  AUTHORS   Yu,H., Rathore,S.S., Gulbranson,D.R. and Shen,J.
  TITLE     The N- and C-terminal domains of tomosyn play distinct roles in
            soluble N-ethylmaleimide-sensitive factor attachment protein
            receptor binding and fusion regulation
  JOURNAL   J Biol Chem 289 (37), 25571-25580 (2014)
   PUBMED   25063806
  REMARK    GeneRIF: We suggest that tomosyn uses its CTD to arrest
            SNARE-dependent fusion reactions, whereas its NTD is required for
            the recruitment of tomosyn to vesicle fusion sites through syntaxin
            interaction.
REFERENCE   5  (bases 1 to 7172)
  AUTHORS   Bielopolski,N., Lam,A.D., Bar-On,D., Sauer,M., Stuenkel,E.L. and
            Ashery,U.
  TITLE     Differential interaction of tomosyn with syntaxin and SNAP25
            depends on domains in the WD40 beta-propeller core and determines
            its inhibitory activity
  JOURNAL   J Biol Chem 289 (24), 17087-17099 (2014)
   PUBMED   24782308
  REMARK    GeneRIF: Unstructured loops in the WD40 domain mediate tomosyn's
            binding to SNAP25 and are involved in tomosyn's organization on the
            plasma membrane.
REFERENCE   6  (bases 1 to 7172)
  AUTHORS   Yizhar,O., Matti,U., Melamed,R., Hagalili,Y., Bruns,D., Rettig,J.
            and Ashery,U.
  TITLE     Tomosyn inhibits priming of large dense-core vesicles in a
            calcium-dependent manner
  JOURNAL   Proc Natl Acad Sci U S A 101 (8), 2578-2583 (2004)
   PUBMED   14983051
  REMARK    GeneRIF: Tomosyn inhibits the priming step and impairs the
            efficiency of vesicle pool refilling in a calcium-dependent manner.
REFERENCE   7  (bases 1 to 7172)
  AUTHORS   Widberg,C.H., Bryant,N.J., Girotti,M., Rea,S. and James,D.E.
  TITLE     Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays
            a role in insulin-stimulated GLUT4 translocation
  JOURNAL   J Biol Chem 278 (37), 35093-35101 (2003)
   PUBMED   12832401
REFERENCE   8  (bases 1 to 7172)
  AUTHORS   Hatsuzawa,K., Lang,T., Fasshauer,D., Bruns,D. and Jahn,R.
  TITLE     The R-SNARE motif of tomosyn forms SNARE core complexes with
            syntaxin 1 and SNAP-25 and down-regulates exocytosis
  JOURNAL   J Biol Chem 278 (33), 31159-31166 (2003)
   PUBMED   12782620
  REMARK    GeneRIF: tomosyn is a soluble SNARE that directly competes with
            synaptobrevin in the formation of SNARE complexes and thus may
            function in down-regulating exocytosis.
REFERENCE   9  (bases 1 to 7172)
  AUTHORS   Yokoyama,S., Shirataki,H., Sakisaka,T. and Takai,Y.
  TITLE     Three splicing variants of tomosyn and identification of their
            syntaxin-binding region
  JOURNAL   Biochem Biophys Res Commun 256 (1), 218-222 (1999)
   PUBMED   10066450
REFERENCE   10 (bases 1 to 7172)
  AUTHORS   Fujita,Y., Shirataki,H., Sakisaka,T., Asakura,T., Ohya,T.,
            Kotani,H., Yokoyama,S., Nishioka,H., Matsuura,Y., Mizoguchi,A.,
            Scheller,R.H. and Takai,Y.
  TITLE     Tomosyn: a syntaxin-1-binding protein that forms a novel complex in
            the neurotransmitter release process
  JOURNAL   Neuron 20 (5), 905-915 (1998)
   PUBMED   9620695
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Oct 4, 2022 this sequence version replaced NM_178345.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF118889.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-692               JAXUCZ010000001.1  6154837-6155528     c
            693-790             JAXUCZ010000001.1  6153519-6153616     c
            791-872             JAXUCZ010000001.1  6109279-6109360     c
            873-973             JAXUCZ010000001.1  6107954-6108054     c
            974-1108            JAXUCZ010000001.1  6092750-6092884     c
            1109-1172           JAXUCZ010000001.1  6091377-6091440     c
            1173-1256           JAXUCZ010000001.1  6087421-6087504     c
            1257-1380           JAXUCZ010000001.1  6085471-6085594     c
            1381-1459           JAXUCZ010000001.1  6076250-6076328     c
            1460-1614           JAXUCZ010000001.1  6064365-6064519     c
            1615-1687           JAXUCZ010000001.1  6063129-6063201     c
            1688-1835           JAXUCZ010000001.1  6059274-6059421     c
            1836-1903           JAXUCZ010000001.1  6058987-6059054     c
            1904-1944           JAXUCZ010000001.1  6058715-6058755     c
            1945-2165           JAXUCZ010000001.1  6053078-6053298     c
            2166-2344           JAXUCZ010000001.1  6052462-6052640     c
            2345-2470           JAXUCZ010000001.1  6046293-6046418     c
            2471-2622           JAXUCZ010000001.1  6044229-6044380     c
            2623-2688           JAXUCZ010000001.1  6034689-6034754     c
            2689-2748           JAXUCZ010000001.1  6030216-6030275     c
            2749-2796           JAXUCZ010000001.1  6030065-6030112     c
            2797-2847           JAXUCZ010000001.1  6022973-6023023     c
            2848-3087           JAXUCZ010000001.1  6015812-6016051     c
            3088-3457           JAXUCZ010000001.1  6014617-6014986     c
            3458-3623           JAXUCZ010000001.1  6014124-6014289     c
            3624-3735           JAXUCZ010000001.1  6012473-6012584     c
            3736-3956           JAXUCZ010000001.1  6007745-6007965     c
            3957-7172           JAXUCZ010000001.1  6002738-6005953     c
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1p13"
     gene            1..7172
                     /gene="Stxbp5"
                     /note="syntaxin binding protein 5"
                     /db_xref="GeneID:81022"
                     /db_xref="RGD:708517"
     exon            1..692
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     CDS             540..3998
                     /gene="Stxbp5"
                     /note="isoform b is encoded by transcript variant b;
                     tomosyn-1; lethal(2) giant larvae protein homolog 3"
                     /codon_start=1
                     /product="syntaxin-binding protein 5 isoform b"
                     /protein_id="NP_848035.1"
                     /db_xref="GeneID:81022"
                     /db_xref="RGD:708517"
                     /translation="MRKFNIRKVLDGLTAGSSSASQQQQQQQHPPGNREPEIQETLQS
                     EHFQLCKTVRHGFPYQPSALAFDPVQKILAVGTQTGALRLFGRPGVECYCQHDSGAAV
                     IQLQFLINEGALVSALADDTLHLWNLRQKRPAVLHSLKFCRERVTFCHLPFQSKWLYV
                     GTERGNIHIVNVESFTLSGYVIMWNKAIELSSKSHPGPVVHISDNPMDEGKLLIGFES
                     GTVVLWDLKSKKADYRYTYDEAIHSVAWHHEGKQFICSHSDGTLTIWNVRSPTKPVQT
                     ITPHGKQLKDGKKPEPCKPILKVEFKTTRSGEPFIILSGGLSYDTVGRRPCLTVMHGK
                     STAVLEMDYSIVDFLTLCETPYPNDFQEPYAVVVLLEKDLVLIDLAQNGYPIFENPYP
                     LSIHESPVTCCEYFADCPVDLIPALYSVGARQKRQGYSKKEWPINGGNWGLGAQSYPE
                     IIITGHADGSIKFWDASAITLQVLYKLKTSKVFEKSRNKDDRQNTDIVDEDPYAIQII
                     SWCPESRMLCIAGVSAHVIIYRFSKQEVVTEVIPMLEVRLLYEINDVETPEGEQPPPL
                     STPVGSSTSQPIPPQSHPSTSSSSSDGLRDNVPCLKVKNSPLKQSPGYQTELVIQLVW
                     VGGEPPQQITSLALNSSYGLVVFGNSNGIAMVDYLQKAVLLNLSTIELYGSNDPYRRE
                     PRSPRKSRQPSGAGLCDITEGTVVPEDRCKSPTSGSSSPHNSDDEQKVNNFIEKVKTQ
                     SRKFSKMVASDLAKMSRKLSLPTDLKPDLDVKDNSFSRSRSSSVTSIDKESREAISAL
                     HFCETFTRKADSSPSPCLWVGTTVGTAFVITLNLPLGPEQRLLQPVIVSPSGTILRLK
                     GAILRMAFLDAAGCLMPPAYEPWTEHNVPEEKDEKEKLKKRRPVSVSPSSSQEISENQ
                     YAVICSEKQAKVISLPTQNCAYKQNITETSFVLRGDIVALSNSVCLACFCANGHIMTF
                     SLPSLRPLLDVYYLPLTNMRIARTFCFANSGQALYLVSPTEIQRLTYSQETCENLQEM
                     LGELFTPVETPEAPNRGFFKGLFGGGAQSLDREELFGESSSGKASRSLAQHIPGPGGI
                     EGVKGAASGVVGELARARLALDERGQKLSDLEERTAAMMSSADSFSKHAHEMMLKYKD
                     KKWYQF"
     exon            693..790
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            791..872
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            873..973
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1108
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1172
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1256
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1380
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1459
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1614
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1687
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1835
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1903
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            1904..1944
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2165
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            2166..2344
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            2345..2470
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            2471..2622
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..2688
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2748
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            2749..2796
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            2797..2847
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            2848..3087
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            3088..3457
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            3458..3623
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            3624..3735
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            3736..3956
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
     exon            3957..7172
                     /gene="Stxbp5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctcgctccg cggaggccca gctgccgcca ccgccgcggc gggcgctgga accgcggctc
       61 gccgcctgct ctgccgggcg ttaagggccg ggggccgagg ttcacgggag gcgacagcga
      121 gcggccgggc ggccggggag agagcgacaa gcggtgcggg gaccgattcc gggtgtccgg
      181 gccatgctgg ccgggcggct gcgctgagca gaggccgagc ccgggacgcg ccgagggact
      241 gcggggctgc gggtcatgga tgcggcggca gcggcgcggg acggcgggag cccggccgcg
      301 accaggtgag gaggcggcgt ccggcgccac tgcagccgcg gcggcctcgg aggaagaggg
      361 ctcgccgccg cgcgcccgcc cgccgtcgct gcccttcctg ttgggattat cttctgctcc
      421 ccgctgcttc ttcgctcccc gcggtcgaag ccgcctctag gcttcagcgg ctcggactcc
      481 ttagcagccg gtgcctctgc tacctgggcc tcgtagctgg gagacccttg ggcgagacca
      541 tgaggaaatt caacatcagg aaggtgctgg acggcctgac cgcaggctcg tcctcggcct
      601 cgcaacagca gcaacagcag cagcacccgc ctgggaaccg ggagcccgag atccaggaga
      661 cgctccagtc cgagcacttc caactctgca agactgttcg ccatggattt ccctatcagc
      721 cctcagccct ggcctttgat cccgttcaga agatcctggc ggtaggaacc cagactggtg
      781 ctttaaggct ctttggtcgt ccaggggtgg aatgttattg ccagcacgac agcggagcgg
      841 cagtgattca actccagttc ctgattaatg agggagccct tgtgagtgcc ttggctgatg
      901 acaccttaca cttgtggaat ttacgtcaga aaaggcctgc tgtgctacat tcactcaaat
      961 tttgcagaga aagggttaca ttttgccatc tgcctttcca gagtaagtgg ctctatgtgg
     1021 gcacggaacg aggtaatata catattgtca atgtggagtc cttcacactc tcaggctacg
     1081 tcattatgtg gaataaagcc atcgaactgt catctaaatc tcacccagga cctgttgtcc
     1141 atataagtga taatcccatg gacgagggga agcttctgat tggctttgaa tctggaacag
     1201 tagtcttatg ggaccttaag tcaaagaagg ctgactacag atacacttac gacgaggcta
     1261 ttcactctgt ggcttggcat catgaaggaa aacagtttat ttgcagtcat tctgatggta
     1321 cattgaccat atggaatgtg aggtccccta ctaaacctgt acagaccatc actcctcacg
     1381 gaaaacagtt aaaggatggg aagaaacccg agccgtgcaa gcctatcctc aaggtggagt
     1441 tcaagacaac aagatcgggg gaacctttta ttattttgtc gggaggctta tcatatgata
     1501 ccgtgggaag aagaccttgc ttaacagtga tgcatgggaa aagcacggca gtgctggaaa
     1561 tggactattc aattgtcgac tttctcacac tctgtgaaac gccatatcca aatgattttc
     1621 aggagccgta tgctgtggtt gttctcctgg agaaggattt agtgctgata gacctggcac
     1681 agaatggata ccctatattt gagaatccct accctttgag tatacacgag tcccctgtta
     1741 catgttgtga atattttgct gattgtcctg tggaccttat tcctgcactt tattctgttg
     1801 gagctagaca gaaacgtcaa ggttacagca aaaaggaatg gcccatcaat ggtggtaatt
     1861 ggggcttggg tgctcaaagt tacccagaaa taattattac agggcatgct gatggctcaa
     1921 ttaaattctg ggatgcttct gcaataactc tacaagtact gtataaatta aaaacatcta
     1981 aagtatttga aaagtcaaga aataaagatg acagacagaa caccgacatt gtagatgaag
     2041 atccatatgc cattcagatc atctcctggt gcccagagag cagaatgctg tgcatagccg
     2101 gagtgtcggc tcatgtcatc atttatagat tcagcaagca ggaagtggtt acagaagtca
     2161 tcccgatgct tgaagtccga ctgttatatg aaataaatga tgtggaaacg ccggagggtg
     2221 agcagccacc ccctttgtcc actcccgtgg gcagctccac ctctcagccc atcccccctc
     2281 agtctcatcc gtctaccagc agcagctcat cggacgggct tcgagataat gtaccgtgtt
     2341 taaaagttaa aaactcacca cttaaacagt ctcccggcta tcaaacagag ctagtcatcc
     2401 agttggtgtg ggtgggtgga gaacccccgc agcagatcac cagcctagca ctcaactctt
     2461 cctacggatt ggtggttttc ggcaactcca atggcattgc aatggttgac tacctccaga
     2521 aagcagtgct gctcaacctc agcaccattg aactatacgg ctcaaatgat ccttatcgga
     2581 gagaaccgag gtcgccccgc aaatctcgac agccttcagg agcgggcctg tgtgatatta
     2641 ccgaaggaac tgtcgtccca gaggatcgct gcaaatctcc gacttccggt tcttcctcgc
     2701 cacacaattc agacgatgaa caaaaagtga ataattttat agaaaaggtg aagacccaaa
     2761 gcagaaagtt ttccaagatg gtagccagtg atctagcaaa gatgtcaagg aaattaagct
     2821 tgccaactga tctaaagcct gatttagatg tgaaagacaa ttccttcagc agatctcgga
     2881 gttcaagtgt gaccagcatt gacaaagagt cccgggaagc catttctgct cttcatttct
     2941 gtgagacttt cacaaggaag gcagactcct ccccctcccc gtgcctgtgg gtgggaacca
     3001 cagtgggaac tgcctttgtc atcacgctga atctccccct ggggcctgag cagagactgc
     3061 ttcagccagt gattgtgtct ccaagcggta ctatattgag gttaaaaggt gcgatcttga
     3121 gaatggcatt tctggatgcc gcgggctgct taatgccacc tgcatacgaa ccctggacag
     3181 agcacaacgt tcctgaagaa aaagacgaaa aggagaaatt gaaaaagcgg cgacctgtct
     3241 cagtgtcccc ctcctcttct caggaaatta gtgaaaacca gtacgcagtg atatgttctg
     3301 aaaagcaagc aaaggtcatc tcactgccaa cccagaactg tgcatacaag cagaacatca
     3361 ctgagacgtc cttcgtgctc cgtggagaca ttgtcgccct gagtaacagt gtctgcctcg
     3421 cctgcttctg tgccaacggc cacattatga ctttcagttt gccgagcttg aggcctctgc
     3481 tggatgtcta ctacctgccc cttaccaaca tgcggatagc caggacattc tgcttcgcca
     3541 acagtgggca agccttatac cttgtttcac ctaccgaaat ccagagactc acctacagtc
     3601 aggagacgtg tgaaaacctt caggagatgc ttggtgagct cttcacgcct gtagaaacac
     3661 cagaagcacc aaacagaggg ttcttcaaag gcttatttgg aggtggtgca caatctcttg
     3721 atagagaaga actgtttgga gagtcatcct cgggaaaggc gtcaaggagc cttgcacagc
     3781 acatcccggg tcctggcggg atcgaaggtg tgaagggagc cgcgtcggga gtggtgggag
     3841 aactggcccg agccaggctg gccctcgacg aaagaggaca gaagctcagc gacttggaag
     3901 agaggactgc agccatgatg tccagtgcag actcgttttc caaacatgct catgagatga
     3961 tgctgaaata caaagataag aagtggtacc agttctgaca agtagcactc agtaagtcca
     4021 gcttcaacca gaaggaaaaa gacgtttcct tgttgaggtc actgatgtat ttgggaaaga
     4081 taacataaaa gggatgcaca ctgctgacag cgtctttccc agcacaatca tgcactgttt
     4141 tacctcagtc acacggcttt aaccgagaac agtccccatg tgtgcaaacc agagcatatg
     4201 gtgtgtgccg gccgcctgcg cattgacaac ggagaactaa gtggggtccc gctgacagat
     4261 gaacggacag aggatgctga gacgtccggg agggctattc cttggatcat tcccgtatta
     4321 aactttcata tgccaaaaga ttttgtgccg ttgtatctgc catcagtgtt tgatccgatg
     4381 tggggagagg caataaatca gaagttgtaa gtctgcaaag gtcacatttg tgtctggact
     4441 gcattgtgaa cagctgtctg cactcgctcg ccctctctca aacagactgc tcatcagtat
     4501 cgtcagtgaa aaagcaacaa acttcccttc tcgttagact ctgctgagtg ctggtcttta
     4561 aggagtcgaa gaagcacaca cagagctcct gtgcgggatc ctcttctact ctcgttctta
     4621 cacaccgtgt tcagttccta tgttttcaaa agcaaaacaa ggtgataggt cacttgtcgt
     4681 ggaaaggtgt ggctcgtgag tgagtggctc atctctgggg agtttccatg cagtggccat
     4741 gcagagtgga aaagtgagaa aacctccatt gtggcgagga gaattcttca atgtcttttg
     4801 tccctgttca catcaattca gctaaaagta gacttgacca aaagttacta gttaaatttg
     4861 taggaaagtt gaaattggct aagtttttaa ttttgcttga atttttataa aatgtttaac
     4921 caaatgttac ccttgcatta tttaattaaa attgctaaaa caacagtgtt ttatcatttc
     4981 agtaccgtgc tcagctccct tttaaaaatg cccttcatct gctcacagtt ccagtacact
     5041 caggtgacga gccagtttaa tcttagtgca cacgctgccg catggaaacc tgcaagtgtc
     5101 tcttgtccag ttacccaaga gtctagtccg atgagctaca aagtatctcc catagaatac
     5161 atcgcacctg tacatatcct tcggaaacac taaaagaaaa aagaaagtgt gttcacttcg
     5221 gtaacttcat ttctcttctg taaccaagcg tgctgggcat tggagggcag tagacaccga
     5281 gtgattggtt caggcagtga gtagggtggc agcctggaga ggaagccccg ccccgctaag
     5341 ctaaagctgc aagggaagat gggaagggaa gtgggggcgc gaacattaca tactgtctcc
     5401 aatgacaggc tgtcccgagt cactgccctt aaaaacgaag aggcacaggt tgctgggaag
     5461 cacggttgtt ttctgttttc tgatgagtaa agtccctgcg aatcaccctc acttctcagc
     5521 ctgttacttg cccgtgtctc tttcaccaag aatccttttg tttacatttg ataacgtgca
     5581 ctaccagata gaaaagttta catccaagtt tactttaaat agcatcaggt aaggactaaa
     5641 tatatgtgat aaagagaact gatcaagata aaaatgtttt taaactataa tcatttttaa
     5701 aatttctgaa tctatagaaa gtgagaaaaa aaattcgttt catgtctgtt ctctgaacat
     5761 tcagcaattt acgaattgct taatttgcat tatatatcgt ccattaaacc catgttccat
     5821 ggtaactcat tggaagaatt gggtcttttt ttaaaggtat agttttatga atatcgatag
     5881 ttgagagttt taaaaagttg aaaagtactg agtgactgag gcactgctcg actctgtgga
     5941 gcctcagctg tttgattcgt tgtattattt gtgtatcaga ttcctttcta cggtcgtttt
     6001 aactgctgta aataagatga atcaaagttg taattattta ctaatagaga agtagataag
     6061 tttaccagag ttggatttta taagaacatt ttaattcatt attttaaatt atctgttttt
     6121 attttaatta atctacatat tgtacacaac agtctaagaa accaggacta gaaagtgcaa
     6181 agtattttta agtagagagt atgagcttcc cagagctgcg cctgatcgtg taaataagaa
     6241 agttccaagt gataacaggg gacggactgt agtctgaaat ggtccgcctc ctcccacccc
     6301 actcctctct ttttgatgca aaaagcagta gccaccagca agccaaagtt tcaggaactt
     6361 tatcaaaaac aaaacaaaca aacaaaaaaa agtcaaaata aaaaatatca aaaaatgaaa
     6421 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgttctctg ccctcatttg
     6481 tctttgattc taaaatttaa gtattcttca attaatttta ttatgggaag agatttctct
     6541 gtatgtacca cataacaaat tcatttcctt ttgtaaacta aaaatcagtc cctgtttgcc
     6601 tagaatgttg ttatttgaag tatctcatct tgtcaaaatc tttatgtgtt tgattatgac
     6661 aacacccgct cacatttcgg ccgtaccact tctaaaagtc ggttacagat tttacataac
     6721 ccgttcctga ttcgaggtct tcataggaaa acactgctac gtgtacacct gcatatcgtt
     6781 ggaagtacgc tgcagttcca cccagaacgg ttcctgaagt ggtcacttag aacttctggt
     6841 gggttcttct gacttctgat gttagttcag gcagacactt gtggctgtga gacaggtctg
     6901 aaagagggct tgtgtttaga acgtgggtgg tgctgggaga taggtgaggg acagaggggt
     6961 ttgctccagg ttttacccta agtcctgtat ttgaatcgtg tgcttttgct caaggcattt
     7021 cgcatgtgac attagtaaag ctatctccaa aggttttttt tgtttgtttg tttttgtatc
     7081 tgtttttctt ttttgtggca aggatttatc ttaacttggg agattttgtt cgtttatatg
     7141 tttctgcgaa ataaagagct ctgaacttta aa
//
