LOCUS       NM_001134749            5880 bp    mRNA    linear   ROD 17-JUL-2023
DEFINITION  Rattus norvegicus STT3 oligosaccharyltransferase complex catalytic
            subunit A (Stt3a), mRNA.
ACCESSION   NM_001134749 XM_001061492 XM_576382
VERSION     NM_001134749.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Roboti P and High S.
  TITLE     The oligosaccharyltransferase subunits OST48, DAD1 and KCP2
            function as ubiquitous and selective modulators of mammalian
            N-glycosylation
  JOURNAL   J Cell Sci 125 (Pt 14), 3474-3484 (2012)
   PUBMED   22467853
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   3  (bases 1 to 5880)
  AUTHORS   Ruiz-Canada C, Kelleher DJ and Gilmore R.
  TITLE     Cotranslational and posttranslational N-glycosylation of
            polypeptides by distinct mammalian OST isoforms
  JOURNAL   Cell 136 (2), 272-283 (2009)
   PUBMED   19167329
REFERENCE   4  (bases 1 to 5880)
  AUTHORS   Faisal A, Kleiner S and Nagamine Y.
  TITLE     Non-redundant role of Shc in Erk activation by cytoskeletal
            reorganization
  JOURNAL   J Biol Chem 279 (5), 3202-3211 (2004)
   PUBMED   14576154
REFERENCE   5  (bases 1 to 5880)
  AUTHORS   Kelleher DJ, Karaoglu D, Mandon EC and Gilmore R.
  TITLE     Oligosaccharyltransferase isoforms that contain different catalytic
            STT3 subunits have distinct enzymatic properties
  JOURNAL   Mol Cell 12 (1), 101-111 (2003)
   PUBMED   12887896
REFERENCE   6  (bases 1 to 5880)
  AUTHORS   Hong G, Deleersnijder W, Kozak CA, Van Marck E, Tylzanowski P and
            Merregaert J.
  TITLE     Molecular cloning of a highly conserved mouse and human integral
            membrane protein (Itm1) and genetic mapping to mouse chromosome 9
  JOURNAL   Genomics 31 (3), 295-300 (1996)
   PUBMED   8838310
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000195.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001134749.3.
            
            On Feb 10, 2021 this sequence version replaced NM_001134749.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC168223.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760476, SAMN16676787
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                JACYVU010000195.1  627139-627198       c
            61-187              JACYVU010000195.1  624639-624765       c
            188-248             JACYVU010000195.1  622914-622974       c
            249-370             JACYVU010000195.1  618734-618855       c
            371-516             JACYVU010000195.1  618241-618386       c
            517-607             JACYVU010000195.1  615194-615284       c
            608-714             JACYVU010000195.1  613785-613891       c
            715-879             JACYVU010000195.1  612117-612281       c
            880-1060            JACYVU010000195.1  610586-610766       c
            1061-1216           JACYVU010000195.1  609260-609415       c
            1217-1308           JACYVU010000195.1  608097-608188       c
            1309-1464           JACYVU010000195.1  604582-604737       c
            1465-1653           JACYVU010000195.1  604022-604210       c
            1654-1770           JACYVU010000195.1  602460-602576       c
            1771-1873           JACYVU010000195.1  602249-602351       c
            1874-2062           JACYVU010000195.1  596256-596444       c
            2063-2178           JACYVU010000195.1  592777-592892       c
            2179-5880           JACYVU010000195.1  587710-591411       c
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q22"
     gene            1..5880
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /note="STT3 oligosaccharyltransferase complex catalytic
                     subunit A"
                     /db_xref="GeneID:500972"
                     /db_xref="RGD:1565793"
     exon            1..60
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            61..187
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     CDS             100..2217
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /EC_number="2.4.99.18"
                     /note="integral transmembrane protein 1; STT3A, subunit of
                     the oligosaccharyltransferase complex (catalytic);
                     integral membrane protein 1; STT3A, catalytic subunit of
                     the oligosaccharyltransferase complex; STT3, subunit of
                     the oligosaccharyltransferase complex, homolog A"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit STT3A"
                     /protein_id="NP_001128221.1"
                     /db_xref="GeneID:500972"
                     /db_xref="RGD:1565793"
                     /translation="MTKLGFLRLSYEKQDTLLKLLILSMAAVLSFSTRLFAVLRFESV
                     IHEFDPYFNYRTTRFLAEEGFYKFHNWFDDRAWYPLGRIIGGTIYPGLMITSAAIYHV
                     LHFFHITIDIRNVCVFLAPLFSSFTTIVTYHLTKELKDAGAGLLAAAMIAVVPGYISR
                     SVAGSYDNEGIAIFCMLLTYYMWIKAVKTGSIYWAAKCALAYFYMVSSWGGYVFLINL
                     IPLHVLVLMLTGRFSHRIYVAYCTVYCLGTILSMQISFVGFQPVLSSEHMAAFGVFGL
                     CQIHAFVDYLRSKLNPQQFEVLFRSVISLVGFVLLTVGALLMLTGKISPWTGRFYSLL
                     DPSYAKNNIPIIASVSEHQPTTWSSYYFDLQLLVFMFPVGLYYCFSNLSDARIFIIMY
                     GVTSMYFSAVMVRLMLVLAPVMCILSGIGVSQVLSTYMKNLDISRPDKKSKKQQDSTY
                     PIKNEVASGMILVMAFFLITYTFHSTWVTSEAYSSPSIVLSARGGDGSRIIFDDFREA
                     YYWLRHNTPEDAKVMSWWDYGYQITAMANRTILVDNNTWNNTHISRVGQAMASTEEKA
                     YEIMRELDVSYVLVIFGGLTGYSSDDINKFLWMVRIGGSTETGRHIKENDYYTPTGEF
                     RVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAY
                     TTEHWLVRIYKVKDLDNRGLSRT"
     exon            188..248
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            249..370
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            371..516
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            517..607
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            608..714
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            715..879
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1060
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1216
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1308
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1464
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1653
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..1770
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1873
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..2062
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2178
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
     exon            2179..5880
                     /gene="Stt3a"
                     /gene_synonym="Itm1; RGD1565793"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggtgcgccgc tgaacggatg gcagggggag cagagtgggt tccggaggag catccgtggg
       61 gtatttgaat atagtcagtt gctacccttt gatgtcaaga tgactaagct tggatttttg
      121 cgattgtcct atgagaagca ggacacactt ctaaagctcc tcatcctgtc gatggctgct
      181 gtgttatctt tttctactcg tctttttgct gtgctcagat ttgaaagtgt catccatgag
      241 ttcgatccgt actttaatta tcggactacc cggtttctgg ctgaggaggg gttttataaa
      301 ttccataact ggtttgatga ccgggcttgg taccctttgg gccgaatcat tggaggaaca
      361 atttacccag gcttaatgat cacttctgct gcaatctacc atgtactcca tttcttccat
      421 atcaccattg acattcggaa tgtctgtgtt ttcctggccc ctcttttctc ctccttcacc
      481 accatcgtta cgtaccacct taccaaagag ctcaaggacg caggagctgg gcttcttgct
      541 gctgccatga ttgctgtagt tcccgggtat atttctcgat ctgtagctgg ctcctatgat
      601 aatgaaggaa ttgctatctt ttgcatgctg cttacttact acatgtggat caaggcagtg
      661 aagactggtt ccatctattg ggctgccaag tgtgctcttg cttatttcta catggtctcg
      721 tcatggggag gctatgtgtt cctgatcaac ttgatccctc ttcacgtcct ggtgctaatg
      781 ctcacaggcc gtttttctca ccggatctac gtagcctact gtactgtgta ctgcctgggg
      841 accattcttt ccatgcagat ctcctttgtt ggattccagc ctgtcctctc atcagaacac
      901 atggcggcct ttggagtgtt tggtctgtgt cagatccatg cttttgtaga ttacctgcgc
      961 agcaagttga atccacagca atttgaagtt cttttccgga gtgttatctc cctggttggc
     1021 ttcgtcctcc tcaccgtggg cgctctcctc atgctgacag gaaagatatc tccctggaca
     1081 gggcgtttct actctctgct ggatccctct tacgctaaga ataacatccc cattattgca
     1141 tctgtgtctg agcaccagcc cacgacctgg tcctcctact attttgatct acagctcctt
     1201 gtcttcatgt ttccagttgg cctctattac tgctttagca acctgtctga tgctcggatt
     1261 tttatcatca tgtatggtgt gaccagcatg tacttctcag ctgtaatggt gcgtctgatg
     1321 ctggtgctgg cacctgttat gtgcattctt tctggcattg gtgtctccca ggtgctgtcc
     1381 acatacatga aaaatctgga cataagtcgc ccagacaaga agagcaagaa gcaacaggat
     1441 tctacttatc ctattaagaa tgaggtggca agtgggatga tactggtcat ggcttttttt
     1501 ctcatcacct acacgtttca ttcgacttgg gtaaccagtg aagcctattc ttctccctcc
     1561 attgtactgt ctgctcgtgg tggggatggc agtaggatca tttttgatga cttccgggaa
     1621 gcatattatt ggcttcgtca caatactcca gaggatgcaa aagtcatgtc atggtgggat
     1681 tatggctacc aaattactgc aatggcaaat cggacaatct tagtggataa taacacatgg
     1741 aataataccc acatttcccg agtagggcag gcaatggcat ccacagaaga aaaagcctac
     1801 gaaatcatga gggagcttga tgtcagctat gtgcttgtca tttttggagg ccttactggg
     1861 tattcttctg atgatatcaa caagtttctg tggatggtcc ggattggagg aagcaccgag
     1921 acaggaagac acatcaagga gaatgactac tatactccaa ctggggaatt ccgtgttgac
     1981 cgtgagggtt caccagtgtt gctcaactgc cttatgtaca aaatgtgtta ctaccgcttt
     2041 gggcaggtct acacagaagc caagcgtcca ccaggctttg accgtgttcg aaatgctgag
     2101 attggtaata aagactttga gcttgatgtc ctggaggaag catataccac agaacattgg
     2161 ctggtcagga tatacaaggt aaaggacctg gataatcgag gcttgtcaag gacataaata
     2221 tcgcattgtg ccctgatatg cttcgcactg agcgcgtctt gttgaggacg ctgaagattt
     2281 ttttttatat gcagtttata agaacagccg gatgggatta gaattgtctg gaagttttgc
     2341 cctggacagt atgggctggg ccaagtgaaa tgatttttat aattctgagc aggttaccaa
     2401 atgaaatgtt aaggctttac tttggtcact taaaaggggg gggggtgggt atttttttta
     2461 aatgtgcctt atttgttttg acttaaattg gctgatatga ggatcacaga agtgaacgga
     2521 tggaagacca aatccatgct ctaggtcccc aaatgaacca aatgggagca ttctttctct
     2581 cctgtcagca aactcaagga ctagctctgg ttctacaaat ataaacaact ttatcacact
     2641 tttttgtttt ttagcaccca ggacaacgat tcccatatat gggtgcttaa taaatttata
     2701 tcaaatgaat aaatgtttct gggaccagag gagtgctgtt tctgcgcaag aaagacagct
     2761 ttcttgctat tatgtctatg ttcttattgt ctatttcttt agaagttctt tggctttata
     2821 aggacagaaa attgctgagt attcccgatc tcgccagtat cctttcaaac taatgacagt
     2881 tattcttttt ctaagcagaa atgtgaacca aactaattca gtagttctta agactgagtg
     2941 aaacatatca ttcttagagg aagacactcc tccaacatca ggttgatgat cagtagatgt
     3001 ttctggaatc agatgtaatt atgtgtacct atatggaatt taggtatttg gtatttaaac
     3061 ctaaaacaca gtaaatggct ctttgctcat ttcctttaga ctgcactaaa tctcttaggg
     3121 ttatccaccg tgaacttctg tttttcttag tgtcagtctg ctgtgttcat ataccacttg
     3181 agacttcatt acttggtttt gaatcccttg ctaaatatat acatagaaag tgggaagttt
     3241 tggtactagc aggtaccttg agaccattgc cagtctgcct gcaaaaaaga gactttaaaa
     3301 acatttaact tcagagcaca gagatcccat agatattttg tgtcaggata aatagctttc
     3361 tgttctatac aataggatat ttgatgcatt ttacaacata ggacataacc actgatgtat
     3421 ataaaccacc ttaagataat tatagtgttt ctatttaaga aaacaggagg aattgtatgg
     3481 ctttgagata gtcaacagtg gtactcaata catatatgct taggtgtact tgtacaccta
     3541 agggcttctc cttcaaacag tttatgtact gtgtatttta actgtggttg gaatttagat
     3601 ttaacagatg atttgatatc ctatatgtaa atacattact tgatttatat gcagaactcc
     3661 tgcctagtgg gaaaggttaa cctgagactt atcaatatga ttaacattat aggttattag
     3721 taagctctaa cctatagtat aaatggggca cccctttttc atatcacttt gactcctggg
     3781 atcttgatac tttctagtct acacagattt ctttttttaa gttgatttca ttttcaggta
     3841 tagtcctcat agtgttaaat ccatctcaaa aacaatttca catacacgcc cacccacaca
     3901 cctacccaca cattccccag actctgcttc cagctctggt gaacctcgca ttgtgcctcc
     3961 tgcctgatca ttagtaactt gctttgttat gttttggtgt tttagtttga ggtaacattg
     4021 attttttttt tttaatccgt ggttctcaac agtgtgggtc atgtcctctt tggcaaacct
     4081 ccatctccaa aaatatttat attgcaattc ataacagcaa aattttagtt aggtagtaac
     4141 aatataagta attttgtgga tgggacatca ccataacatg aggaactgta ttaaagagtt
     4201 gcagcatcag gaaggttgaa taccactgcc ttatgttttg tgacaccatt tttactatta
     4261 tctattacag ttctaattat tttaccttct ggctattctt agttaagatg tgttactttt
     4321 tttttttttt gttctttttt tcggagctgg ggaccgaacc cagggccttg cgcttcctag
     4381 gcaagcgctc taccactgag ctaaatcccc aaccccactt tttttttttt ttttttaaag
     4441 atagttccat gtagctcagg ccagcagctc tgaactcaag atatagatga ggctggcttt
     4501 ggactcttga tcttcctgct tctacctccc agttgtgggc tactacagct agttagttac
     4561 tatttttcct atataatttt ctaaaggaaa tactttaatt tttttggtat ttgttctctc
     4621 tcctactcct ttaccactct agctagttct ccctcatttg tgcctatttt ctcttactga
     4681 tcgaacacat tcattgaaca actttgggtg tttgaaacag tatcttttgt agctagggct
     4741 ggctttgaac tcctgattca cctgcctctg tttgccaaat gctggtatta agctatatgc
     4801 caccatacct aattatattg ggttaatttt aagaatgtct ttgctttttc agcttgaaat
     4861 aactcaagtt tgttgaattg tattatttca ctgttgtatt tgttgatctt tgccattttc
     4921 agtgatttgc catttcttag taccttatat gtagagggga tacatttctg cagctcttct
     4981 atatcatatt taatgcacag ctgggtatag cactccagac tggcgctatt tacattataa
     5041 aggcactggc ccattttctt aatgttgaga aatggcagtt attttgatga gtatttgtgt
     5101 gttctttgcc ccagatctct ttgttttgtt tttttttttt ttaattgtgg tgctgggtac
     5161 tctaaatttt catgtatgca tagtcaaatt tttcagttta aaaaaaaaca acactagtta
     5221 tatatagatt tctccaatct ctttctgata aggctaatta aaacttttac attttaggca
     5281 acatggtatg acactttaaa tatttacttt tgtgtgtgtg tgtgtttggg tattttacct
     5341 gcatatatgt gtgtgtatca tgtgtgtacc tggtgcccac agaatccaaa ggaaggtatc
     5401 agagtcctgg aactggagtt ataaacagtc atgagttgcc gtgtgggtgc tgggaattga
     5461 acactttgga agaacagctg ttggtcttta ctgttgaatc agctccccag tccatgataa
     5521 atggggcttt ctgagttttc taaaagtttc ttagttgctt ttctttcttc cactttttgg
     5581 gagatttcct tagctgtatt tgtggtcatg ctgttttccg tccctccgtc cctccctccc
     5641 tgtctctttc ttcctactcc ttcttccctc tctttcccct tttagctatt aacgcatagg
     5701 tttaagccta gggccctgtg catgctagct aagcactcta tcagtgggca acatctctta
     5761 atttcaacca tattttcaaa taaatttttc ttttctgcct tcatatttct aaagtcttaa
     5821 gaactctttt gactgttcca ttgttttgct gcctttccaa ttaaagactt ttccaaattc
//
