LOCUS       NM_019362               3551 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus serine threonine kinase 39 (Stk39), mRNA.
ACCESSION   NM_019362 XM_001057901
VERSION     NM_019362.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Liu,J., Shelton,E.L., Crescenzi,R., Colvin,D.C., Kirabo,A.,
            Zhong,J., Delpire,E.J., Yang,H.C. and Kon,V.
  TITLE     Kidney Injury Causes Accumulation of Renal Sodium That Modulates
            Renal Lymphatic Dynamics
  JOURNAL   Int J Mol Sci 23 (3), 1428 (2022)
   PUBMED   35163352
  REMARK    GeneRIF: Kidney Injury Causes Accumulation of Renal Sodium That
            Modulates Renal Lymphatic Dynamics.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Gong,Y., Wu,M., Gao,F., Shi,M., Gu,H., Gao,R., Dang,B.Q. and
            Chen,G.
  TITLE     Inhibition of the p-SPAK/p-NKCC1 signaling pathway protects the
            blood-brain barrier and reduces neuronal apoptosis in a rat model
            of surgical brain injury
  JOURNAL   Mol Med Rep 24 (4) (2021)
   PUBMED   34396440
  REMARK    GeneRIF: Inhibition of the pSPAK/pNKCC1 signaling pathway protects
            the bloodbrain barrier and reduces neuronal apoptosis in a rat
            model of surgical brain injury.
REFERENCE   3  (bases 1 to 3551)
  AUTHORS   Zhang,J., Gao,G., Begum,G., Wang,J., Khanna,A.R., Shmukler,B.E.,
            Daubner,G.M., de Los Heros,P., Davies,P., Varghese,J.,
            Bhuiyan,M.I., Duan,J., Zhang,J., Duran,D., Alper,S.L., Sun,D.,
            Elledge,S.J., Alessi,D.R. and Kahle,K.T.
  TITLE     Functional kinomics establishes a critical node of volume-sensitive
            cation-Cl- cotransporter regulation in the mammalian brain
  JOURNAL   Sci Rep 6, 35986 (2016)
   PUBMED   27782176
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3551)
  AUTHORS   Kochl,R., Thelen,F., Vanes,L., Brazao,T.F., Fountain,K., Xie,J.,
            Huang,C.L., Lyck,R., Stein,J.V. and Tybulewicz,V.L.
  TITLE     WNK1 kinase balances T cell adhesion versus migration in vivo
  JOURNAL   Nat Immunol 17 (9), 1075-1083 (2016)
   PUBMED   27400149
  REMARK    Erratum:[Nat Immunol. 2017 Jan 19;18(2):246. doi:
            10.1038/ni0217-246a.. PMID: 28102214]
REFERENCE   5  (bases 1 to 3551)
  AUTHORS   Fezai,M., Elvira,B., Borras,J., Ben-Attia,M., Hoseinzadeh,Z. and
            Lang,F.
  TITLE     Negative regulation of the creatine transporter SLC6A8 by SPAK and
            OSR1
  JOURNAL   Kidney Blood Press Res 39 (6), 546-554 (2014)
   PUBMED   25531585
REFERENCE   6  (bases 1 to 3551)
  AUTHORS   Vitari,A.C., Deak,M., Morrice,N.A. and Alessi,D.R.
  TITLE     The WNK1 and WNK4 protein kinases that are mutated in Gordon's
            hypertension syndrome phosphorylate and activate SPAK and OSR1
            protein kinases
  JOURNAL   Biochem J 391 (Pt 1), 17-24 (2005)
   PUBMED   16083423
  REMARK    GeneRIF: WNK1 and WNK4 both interacted with SPAK as well as a
            closely related kinase, termed oxidative stress response kinase-1
            (OSR1).
REFERENCE   7  (bases 1 to 3551)
  AUTHORS   Dowd,B.F. and Forbush,B.
  TITLE     PASK (proline-alanine-rich STE20-related kinase), a regulatory
            kinase of the Na-K-Cl cotransporter (NKCC1)
  JOURNAL   J Biol Chem 278 (30), 27347-27353 (2003)
   PUBMED   12740379
  REMARK    GeneRIF: roles in phosphorylation and activation of the Na-K-Cl
            cotransporter (NKCC1)
REFERENCE   8  (bases 1 to 3551)
  AUTHORS   Piechotta,K., Lu,J. and Delpire,E.
  TITLE     Cation chloride cotransporters interact with the stress-related
            kinases Ste20-related proline-alanine-rich kinase (SPAK) and
            oxidative stress response 1 (OSR1)
  JOURNAL   J Biol Chem 277 (52), 50812-50819 (2002)
   PUBMED   12386165
REFERENCE   9  (bases 1 to 3551)
  AUTHORS   Johnston,A.M., Naselli,G., Gonez,L.J., Martin,R.M., Harrison,L.C.
            and DeAizpurua,H.J.
  TITLE     SPAK, a STE20/SPS1-related kinase that activates the p38 pathway
  JOURNAL   Oncogene 19 (37), 4290-4297 (2000)
   PUBMED   10980603
REFERENCE   10 (bases 1 to 3551)
  AUTHORS   Ushiro,H., Tsutsumi,T., Suzuki,K., Kayahara,T. and Nakano,K.
  TITLE     Molecular cloning and characterization of a novel Ste20-related
            protein kinase enriched in neurons and transporting epithelia
  JOURNAL   Arch Biochem Biophys 355 (2), 233-240 (1998)
   PUBMED   9675032
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from D88190.1.
            
            On Jun 25, 2006 this sequence version replaced XM_001057901.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D88190.1, AF099990.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q21"
     gene            1..3551
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /note="serine threonine kinase 39"
                     /db_xref="GeneID:54348"
                     /db_xref="RGD:621643"
     exon            1..651
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     CDS             417..2078
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /EC_number="2.7.11.1"
                     /function="Ste20-related protein kinase"
                     /note="PS/TK; PSTK1; ste-20-related kinase;
                     serine/threonine-protein kinase 39; pancreatic
                     serine/threonine-protein kinase; Ste-20 related kinase"
                     /codon_start=1
                     /product="STE20/SPS1-related proline-alanine-rich protein
                     kinase"
                     /protein_id="NP_062235.1"
                     /db_xref="GeneID:54348"
                     /db_xref="RGD:621643"
                     /translation="MAEPSGSPVHVQLPQQAAPVTAAAAAPAAATSAPAPAPAPAAPA
                     APAPAPAAAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKR
                     INLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIK
                     YIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIA
                     DFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELA
                     TGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKR
                     PTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWE
                     WSDDEMDEKSEEGKAAASQEKSRRVKEENPEISVNAGGIPEQIQSLSVHDSQGQPNAN
                     EDYREGPCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIV
                     AANLQKIVDDPKALKTLTFKLASGCDGAEIPDEVKLIGFAQLSVS"
     exon            652..764
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            765..873
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1015
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1071
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1181
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1283
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1417
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1466
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1532
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1655
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1685
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..1748
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1816
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..1869
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1870..1938
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            1939..2003
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..3551
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3551
                     /gene="Stk39"
                     /gene_synonym="Spak"
                     /note="9 a nucleotides"
ORIGIN      
        1 gtctagggcg gaggcgcggc tgggtaggaa gggagcctgg cgatccccga aagcacccct
       61 gaagccgcca ggctccgagc gcgtggaaac ttccctggcg gcaagcaggg cgggaggggg
      121 cggcggttcc ccccagcgct gagagcaccg cgcgcgcgag gatcggggcg cgcggcgggc
      181 gcggcggccg ccgcctcctc ccgtccccgc ctccgcggcg cagcctcctc cctccgcccg
      241 gctcggcctc gggctccctc ccctcgtggc aggctccggc ggcggcggcg gcggcggctg
      301 cggcggacgg ggagggcgtg cgccggtcga gaggtgtggg cgacgaggct agggaagttt
      361 caagtggaag gtcgtccgcc ggccggcgcg tcccctcact ctcctcccgc agcatcatgg
      421 cggagccgag cggctcgccc gtgcacgtcc agcttcccca gcaggcggcc ccggtgacag
      481 ccgcggcggc ggctccggcg gccgcgacat cagcaccggc cccggccccg gccccggcgg
      541 cgcccgcagc cccggccccg gctccagctg cggctccagc cccggccccg gcagctcagg
      601 cggtcggctg gcccatctgc agggacgcgt acgagctcca ggaggttatc ggcagtggag
      661 cgaccgccgt ggttcaggca gccctgtgca aacccaggca agaacgcgta gccataaagc
      721 ggatcaactt ggaaaagtgc cagacgagta tggatgaact cttaaaagaa attcaagcca
      781 tgagccagtg cagccatccc aacgtagtga cttattatac ttcctttgtg gtcaaagatg
      841 aactttggct ggtcatgaaa ttactaagtg gaggttccat gttggatatc atcaaataca
      901 tcgtcaatcg gggagagcat aagaatggtg tcctggaaga ggcaataatc gcaacaatcc
      961 ttaaggaggt tttggaagga ttagactatc tgcatagaaa tggtcagatc catagggatt
     1021 tgaaagctgg caatattctt ctgggtgagg atggatcagt acagatagca gattttggag
     1081 taagtgcatt cttagcaaca gggggtgatg tcacaaggaa taaagtcaga aaaacatttg
     1141 ttggtacccc atgttggatg gcccctgagg tcatggaaca ggtgagaggc tatgacttca
     1201 aggctgacat gtggagtttt ggaataacag ccattgaatt agcaacggga gcagcgcctt
     1261 accacaaata ccctccaatg aaagtgctaa tgttgacttt gcaaaatgac ccgcccactt
     1321 tagaaaccgg cgtagaggat aaagaaatga tgaaaaaata cggcaagtcc ttcagaaagt
     1381 tactttcact gtgtctccag aaagatcctt ccaaaaggcc cacagcagca gaacttttaa
     1441 aatgcaaatt cttccagaaa gccaagaaca gagagtacct gatcgagaag ttgctgacac
     1501 gaacaccaga catagcccaa agagccaaga aggtcaggcg agttcctggg tcaagcggtc
     1561 accttcacaa gactgaagat ggcgactggg agtggagtga tgatgagatg gatgagaaga
     1621 gtgaggaggg gaaagcggct gcctctcaag agaagtcacg aagagtaaaa gaagagaacc
     1681 cagagatctc ggtgaacgct ggtggcatcc ccgagcaaat acagtccctc tccgtgcacg
     1741 actctcaggg ccaaccaaat gctaatgaag actacagaga aggtccttgt gcggtcaacc
     1801 ttgttttaag attaagaaac tccagaaagg aacttaatga catacgattt gagtttactc
     1861 caggaagaga tacagcagat ggtgtgtctc aggagctctt ctctgctggc ttggttgacg
     1921 gtcatgatgt agttatagtg gctgctaatt tacagaagat tgtagatgac cccaaagctt
     1981 taaaaacgtt gacatttaag ttggcttctg gctgtgatgg ggcggagatt cctgacgaag
     2041 tgaagctgat cgggttcgcc cagttgagtg tgagctgatg tatgtccctt gctgtcaccc
     2101 tcatctgtcg tgccctcccc cccttccagc cccctctttg cctccttcct ctcaccccct
     2161 cactcccaaa tcccagcaaa taagaagatt gtgaagaggc tggcttcgat gaagtgggat
     2221 aaaaaaaata ataattttaa aaaaaaactc cgagcactct gagttctgct ttattctcca
     2281 gcaatccaca gtgtgagcac aggcaagtgc tgcaatagtg cattgctgat ttctttccag
     2341 agagctattt actattctta gcagtcaatt tggatagccc ttaagtgagg agtctgaaac
     2401 acactcaggt ggtcctatct cttggcaaca aaaggaatct tctaccagaa gccttttctc
     2461 ctcttactgt tgtatttctg tttaataatt aataataatt aattgtacat ctgacaatac
     2521 tgcctctttt atgttgtctt agaaattaat atacttataa aattaagatt tattagccag
     2581 acttgaattc tagttttaaa actgactgtg aattttattt ttcatatatt tatgctttac
     2641 acaccttagc tatgagggaa aaagttttga ttataatgct ttccgcggtt gatctcttgt
     2701 tatttaaaca aatggtttca ggtcaatctt tggagtattt gtagcttcta atttttgaaa
     2761 tgactgaatt gaaaatttga atgctttctc ttttggttgg tttgcctaaa atccagtcca
     2821 caatccagtc gtctcttggg agagggaggt gccttgtaaa ctttcatact tcgaatgtgc
     2881 ctgagactgc ttaaccctgg actagtctca gatctcaaac ccgcactaca catggaggca
     2941 tacgtttgct tcatctggac atttagaata ctgtacctgt gctgccgttc tgttagatcg
     3001 ctgactacat ccccgtgtct gattcggctc tccttaggtg ataggattca ttgtttgtaa
     3061 ggcgataaac ttgaccacac cagaatctga gttttacttg aaactctgca gaatacacag
     3121 ggggtatgaa aaacaaaaca aaacaaaaca gaaagatttt tttgtgcaat ggcaaaaggt
     3181 aaaaatgctg taaacgttcg agaataaata tttccaaccc aagaacctcc tgcttgacta
     3241 tgtattatga acctaacaaa ccttaggatt ttcaaatttg gagtcaaaac cctcaagaag
     3301 gtggctccgg ggatggcacc attgctcctt acaagctaac cctagatgcg gcagctcttt
     3361 aacgtacttt aaaaagcaaa tatatattac taaggaaaga agttatttat aattgccttg
     3421 tcataatcgt taaggtgttc tagaaccatt tgcatacaat ttaatgtaat tccattccat
     3481 tctattgttt acacaattac tcaaagatgg ctgcaaaggt aaaaggaaaa ataaaagcgt
     3541 cttgcacagt g
//
