LOCUS       NP_001388305            1071 aa            linear   ROD 03-APR-2024
DEFINITION  SLIT-ROBO Rho GTPase-activating protein 2 isoform 1 [Rattus
            norvegicus].
ACCESSION   NP_001388305 XP_006249844
VERSION     NP_001388305.1
DBSOURCE    REFSEQ: accession NM_001401376.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 1071)
  AUTHORS   Ho,N.T.T., Kutzner,A. and Heese,K.
  TITLE     A Novel Divergent Gene Transcription Paradigm-the Decisive,
            Brain-Specific, Neural |-Srgap2-Fam72a-| Master Gene Paradigm
  JOURNAL   Mol Neurobiol 56 (8), 5891-5899 (2019)
   PUBMED   30685845
  REMARK    GeneRIF: These data provide new insights into the specific
            regulation of the |-Srgap2-Fam72a-| master gene.
REFERENCE   2  (residues 1 to 1071)
  AUTHORS   Jiao,Q., Wang,L., Zhang,Z., Wang,Y., Yan,H., Ma,W., Jin,W., Lu,H.
            and Liu,Y.
  TITLE     Dynamic expression of srGAP2 in cell nuclei and cytoplasm during
            the differentiation of rat neural stem cells in vitro
  JOURNAL   Mol Med Rep 14 (5), 4599-4605 (2016)
   PUBMED   27748913
  REMARK    GeneRIF: The translocation of srGAP2 in the cytoplasm and cell
            nucleus in different cell types may function as a director in
            decisions regarding cell fate.
REFERENCE   3  (residues 1 to 1071)
  AUTHORS   Guez-Haddad,J., Sporny,M., Sasson,Y., Gevorkyan-Airapetov,L.,
            Lahav-Mankovski,N., Margulies,D., Radzimanowski,J. and Opatowsky,Y.
  TITLE     The Neuronal Migration Factor srGAP2 Achieves Specificity in Ligand
            Binding through a Two-Component Molecular Mechanism
  JOURNAL   Structure 23 (11), 1989-2000 (2015)
   PUBMED   26365803
REFERENCE   4  (residues 1 to 1071)
  AUTHORS   Charrier,C., Joshi,K., Coutinho-Budd,J., Kim,J.E., Lambert,N., de
            Marchena,J., Jin,W.L., Vanderhaeghen,P., Ghosh,A., Sassa,T. and
            Polleux,F.
  TITLE     Inhibition of SRGAP2 function by its human-specific paralogs
            induces neoteny during spine maturation
  JOURNAL   Cell 149 (4), 923-935 (2012)
   PUBMED   22559944
REFERENCE   5  (residues 1 to 1071)
  AUTHORS   Mason,F.M., Heimsath,E.G., Higgs,H.N. and Soderling,S.H.
  TITLE     Bi-modal regulation of a formin by srGAP2
  JOURNAL   J Biol Chem 286 (8), 6577-6586 (2011)
   PUBMED   21148482
REFERENCE   6  (residues 1 to 1071)
  AUTHORS   Guo,S. and Bao,S.
  TITLE     srGAP2 arginine methylation regulates cell migration and cell
            spreading through promoting dimerization
  JOURNAL   J Biol Chem 285 (45), 35133-35141 (2010)
   PUBMED   20810653
REFERENCE   7  (residues 1 to 1071)
  AUTHORS   Guerrier,S., Coutinho-Budd,J., Sassa,T., Gresset,A., Jordan,N.V.,
            Chen,K., Jin,W.L., Frost,A. and Polleux,F.
  TITLE     The F-BAR domain of srGAP2 induces membrane protrusions required
            for neuronal migration and morphogenesis
  JOURNAL   Cell 138 (5), 990-1004 (2009)
   PUBMED   19737524
REFERENCE   8  (residues 1 to 1071)
  AUTHORS   Yao,Q., Jin,W.L., Wang,Y. and Ju,G.
  TITLE     Regulated shuttling of Slit-Robo-GTPase activating proteins between
            nucleus and cytoplasm during brain development
  JOURNAL   Cell Mol Neurobiol 28 (2), 205-221 (2008)
   PUBMED   17710530
  REMARK    GeneRIF: results suggest that srGAPs are not only involved in the
            regulation of the Slit-Robo signal transduction, but also in
            neuronal development and that the translocation of srGAPs is
            important for their functions.
REFERENCE   9  (residues 1 to 1071)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On Feb 10, 2022 this sequence version replaced XP_006249844.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.50932.1,
                                           SRR26360176.82240.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q13"
     Protein         1..1071
                     /product="SLIT-ROBO Rho GTPase-activating protein 2
                     isoform 1"
                     /calculated_mol_wt=120747
     Region          26..289
                     /region_name="F-BAR_srGAP2"
                     /note="The F-BAR (FES-CIP4 Homology and
                     Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating
                     Protein 2; cd07682"
                     /db_xref="CDD:153366"
     Site            order(31,34,41,44..45,48,51..52,55..56,59,63,66,70,109,
                     112,222,226,251,255,258,261..262,265,269,272,276,281,
                     284..285,288)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153366"
     Region          181..211
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (D4A208.1)"
     Site            206
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Site            427
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Region          487..674
                     /region_name="RhoGAP_srGAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in srGAPs. srGAPs are
                     components of the intracellular part of Slit-Robo
                     signalling pathway that is important for axon guidance and
                     cell migration. srGAPs contain an...; cd04383"
                     /db_xref="CDD:239848"
     Site            500
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q91Z67; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Site            order(527,566,570,639,642..643,665)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239848"
     Site            527
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239848"
     Site            691
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Site            695
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q91Z67; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Region          703..726
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (D4A208.1)"
     Site            724
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O75044; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Region          732..784
                     /region_name="SH3_srGAP1-3"
                     /note="Src homology 3 domain of Slit-Robo GTPase
                     Activating Proteins 1, 2, and 3; cd11955"
                     /db_xref="CDD:212888"
     Site            order(737,739,742,746,764..765,778,780..781)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212888"
     Region          794..820
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (D4A208.1)"
     Site            795
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O75044; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Region          835..936
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (D4A208.1)"
     Site            916
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O75044; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Site            927
                     /site_type="methylation"
                     /note="Symmetric dimethylarginine, by PRMT5.
                     /evidence=ECO:0000250|UniProtKB:O75044; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Site            930
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O75044; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Region          963..>1065
                     /region_name="PHA03269"
                     /note="envelope glycoprotein C; Provisional"
                     /db_xref="CDD:165527"
     Region          983..1012
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (D4A208.1)"
     Site            990
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q91Z67; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Site            994
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O75044; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Site            1013
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O75044; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Site            1027
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O75044; propagated from
                     UniProtKB/Swiss-Prot (D4A208.1)"
     Region          1029..1071
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (D4A208.1)"
     CDS             1..1071
                     /gene="Srgap2"
                     /gene_synonym="RGD1566016"
                     /coded_by="NM_001401376.1:733..3948"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="GeneID:360840"
                     /db_xref="RGD:1566016"
ORIGIN      
        1 mtspakfkkd keiiaeydtq vkeiraqlte qmkcldqqce lrvqllqdlq dffrkkaeie
       61 mdysrnlekl aerflaktrs tkdqqfkkdq nvlspvncwn lllnqvkres rdhttlsdiy
      121 lnniiprfvq vsedsgrlfk kskevgqqlq ddlmkvlnel ysvmktyhmy nadsisaqsk
      181 lkeaekqeek qigksvkqed rqtprspdst anvrieekhv rrssvkkiek mkekrqakyt
      241 enklkaikar neyllaleat nasvfkyyih dlsdiidqcc dlgyhaslnr alrtflsael
      301 nleqskhegl daienavenl datsdkqrlm emynnvfcpp mkfefqphmg dmasqlcaqq
      361 pvqselvqrr qqlqsrlstl kieneevkkt meatlqtiqd ivtvedfdvs dcfqysnsme
      421 svkstvsetf mskpsiakrr anqqeteqfy ftkmkeyleg rnlitklqak hdllqktlge
      481 sqrtdcslar rsstvrkqds sqaiplvves cirfisrhgl rhegvfrvsg sqvevndikn
      541 afergedpla gdqndhdmds iagvlklyfr glehplfpkd ifhdliacvt mdnlqeravh
      601 irkvllvlpk ptliimrylf aflnhlsqfs eenmmdpynl aicfgpslms vpeghdqvsc
      661 qahvnelikt iiiqhenifp nprelegpiy srggsmedyc dsthgetisa edstqdvtae
      721 hhtsddecep ieaiakfdyv grtarelsfk kgaslllyqr asddwwegrh ngidgliphq
      781 yivvqdtedg vversspkse ievmseppee kvtartgasc psgghvadiy laninkqrkr
      841 pesgsirkaf rsdshglgss ltdssspgvg ascrpssqpi msqnlpkegp dkcsisghgs
      901 lnsisrhssl knrmdspqir ktatagrsks fnnhrpmdpe viaqdieatm nsalnelqel
      961 erqssakhtp dvvldtlepl ktspvvapts epssplhtql lkdpepafqr sastagdiac
     1021 afrpvksvkm aapvkppatr pkptvfpktn atspgvnssa spqstdksct v
//
