LOCUS       NM_017136               2085 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus squalene epoxidase (Sqle), mRNA.
ACCESSION   NM_017136
VERSION     NM_017136.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Padyana,A.K., Gross,S., Jin,L., Cianchetta,G., Narayanaswamy,R.,
            Wang,F., Wang,R., Fang,C., Lv,X., Biller,S.A., Dang,L.,
            Mahoney,C.E., Nagaraja,N., Pirman,D., Sui,Z., Popovici-Muller,J.
            and Smolen,G.A.
  TITLE     Structure and inhibition mechanism of the catalytic domain of human
            squalene epoxidase
  JOURNAL   Nat Commun 10 (1), 97 (2019)
   PUBMED   30626872
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Polycarpou-Schwarz,M., Gross,M., Mestdagh,P., Schott,J.,
            Grund,S.E., Hildenbrand,C., Rom,J., Aulmann,S., Sinn,H.P.,
            Vandesompele,J. and Diederichs,S.
  TITLE     The cancer-associated microprotein CASIMO1 controls cell
            proliferation and interacts with squalene epoxidase modulating
            lipid droplet formation
  JOURNAL   Oncogene 37 (34), 4750-4768 (2018)
   PUBMED   29765154
REFERENCE   3  (bases 1 to 2085)
  AUTHORS   Marmugi,A., Lasserre,F., Beuzelin,D., Ducheix,S., Huc,L.,
            Polizzi,A., Chetivaux,M., Pineau,T., Martin,P., Guillou,H. and
            Mselli-Lakhal,L.
  TITLE     Adverse effects of long-term exposure to bisphenol A during
            adulthood leading to hyperglycaemia and hypercholesterolemia in
            mice
  JOURNAL   Toxicology 325, 133-143 (2014)
   PUBMED   25168180
REFERENCE   4  (bases 1 to 2085)
  AUTHORS   Abe,I., Abe,T., Lou,W., Masuoka,T. and Noguchi,H.
  TITLE     Site-directed mutagenesis of conserved aromatic residues in rat
            squalene epoxidase
  JOURNAL   Biochem Biophys Res Commun 352 (1), 259-263 (2007)
   PUBMED   17112472
  REMARK    GeneRIF: aromatic residues are likely to be located at the
            substrate-binding domain of the active-site, and control the
            stereochemical course of the squalene epoxidase reaction
REFERENCE   5  (bases 1 to 2085)
  AUTHORS   Waterham,H.R.
  TITLE     Defects of cholesterol biosynthesis
  JOURNAL   FEBS Lett 580 (23), 5442-5449 (2006)
   PUBMED   16876788
  REMARK    Review article
REFERENCE   6  (bases 1 to 2085)
  AUTHORS   Nagai,M., Sakakibara,J., Nakamura,Y., Gejyo,F. and Ono,T.
  TITLE     SREBP-2 and NF-Y are involved in the transcriptional regulation of
            squalene epoxidase
  JOURNAL   Biochem Biophys Res Commun 295 (1), 74-80 (2002)
   PUBMED   12083769
  REMARK    GeneRIF: functional promotor regions involved in transcription was
            determined
REFERENCE   7  (bases 1 to 2085)
  AUTHORS   Abe,I., Kashiwagi,Y., Noguchi,H., Tanaka,T., Ikeshiro,Y. and
            Kashiwada,Y.
  TITLE     Ellagitannins and hexahydroxydiphenoyl esters as inhibitors of
            vertebrate squalene epoxidase
  JOURNAL   J Nat Prod 64 (8), 1010-1014 (2001)
   PUBMED   11520216
REFERENCE   8  (bases 1 to 2085)
  AUTHORS   Matzno,S., Yamauchi,T., Gohda,M., Ishida,N., Katsuura,K.,
            Hanasaki,Y., Tokunaga,T., Itoh,H. and Nakamura,N.
  TITLE     Inhibition of cholesterol biosynthesis by squalene epoxidase
            inhibitor avoids apoptotic cell death in L6 myoblasts
  JOURNAL   J Lipid Res 38 (8), 1639-1648 (1997)
   PUBMED   9300786
REFERENCE   9  (bases 1 to 2085)
  AUTHORS   Sakakibara,J., Watanabe,R., Kanai,Y. and Ono,T.
  TITLE     Molecular cloning and expression of rat squalene epoxidase
  JOURNAL   J Biol Chem 270 (1), 17-20 (1995)
   PUBMED   7814369
REFERENCE   10 (bases 1 to 2085)
  AUTHORS   Ono,T., Nakazono,K. and Kosaka,H.
  TITLE     Purification and partial characterization of squalene epoxidase
            from rat liver microsomes
  JOURNAL   Biochim Biophys Acta 709 (1), 84-90 (1982)
   PUBMED   6817796
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC097330.1.
            
            On Jun 10, 2007 this sequence version replaced NM_017136.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC097330.1, D37920.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q33"
     gene            1..2085
                     /gene="Sqle"
                     /note="squalene epoxidase"
                     /db_xref="GeneID:29230"
                     /db_xref="RGD:3755"
     exon            1..356
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..1790
                     /gene="Sqle"
                     /EC_number="1.14.14.17"
                     /note="SE"
                     /codon_start=1
                     /product="squalene monooxygenase"
                     /protein_id="NP_058832.1"
                     /db_xref="GeneID:29230"
                     /db_xref="RGD:3755"
                     /translation="MWTFLGIATFTYFYKKCGDVTLANKELLLCVLVFLSLGLVLSYR
                     CRHRNGGLLGRHQSGSQFAAFSDILSALPLIGFFWAKSPPESEKKEQLESKRRRKEVN
                     LSETTLTGAATSVSTSSVTDPEVIIIGSGVLGSALATVLSRDGRTVTVIERDLKEPDR
                     ILGECLQPGGYRVLRELGLGDTVESLNAHHIHGYVIHDCESRSEVQIPYPVSENNQVQ
                     SGVAFHHGKFIMSLRKAAMAEPNVKFIEGVVLRLLEEDDAVIGVQYKDKETGDTKELH
                     APLTVVADGLFSKFRKNLISNKVSVSSHFVGFIMKDAPQFKANFAELVLVDPSPVLIY
                     QISPSETRVLVDIRGELPRNLREYMTEQIYPQIPDHLKESFLEACQNARLRTMPASFL
                     PPSSVNKRGVLLLGDAYNLRHPLTGGGMTVALKDIKIWRQLLKDIPDLYDDAAIFQAK
                     KSFFWSRKRSHSFVVNVLAQALYELFSATDDSLRQLRKACFLYFKLGGECLTGPVGLL
                     SILSPDPLLLIRHFFSVAVYATYFCFKSEPWATKPRALFSSGAILYKACSIIFPLIYS
                     EMKYLVH"
     exon            357..609
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            610..790
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            791..887
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1001
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1173
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1269
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1412
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1509
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1597
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..2059
                     /gene="Sqle"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caaaaaaacc aaaccccaaa cccccaaatc tcagtcttag ctggtgccac caaagaagct
       61 tttgagccat gtggactttt cttggaattg ctactttcac ctatttttat aagaaatgcg
      121 gggatgtcac cctggccaac aaggagctcc tgctgtgcgt actggtgttc ctgtcgctgg
      181 gcctggtgct ctcctaccgc tgtcgccatc gaaacggggg cctccttggc cgccatcaga
      241 gcggctccca gttcgctgcc ttctcagata ttctctctgc tttgcctctt attggcttct
      301 tctgggccaa atcaccccct gaatcagaaa agaaagaaca gctggagtcc aagaggcgca
      361 gaaaagaagt caatctgtca gagacgacgt taacgggagc agctacctca gtatcgacat
      421 catctgtgac ggacccagaa gtgatcatca tagggtctgg tgtacttggc tctgctttgg
      481 caacagtact ctctagagat ggaagaacgg tgacagtaat cgagagagat ttaaaagagc
      541 ctgacagaat acttggagag tgtctgcagc ccggtggcta tcgagttctt cgagagctgg
      601 gccttggaga tacagtagaa agtcttaatg cccatcatat acacggctac gtaattcatg
      661 actgtgaaag caggtctgaa gttcaaattc catacccggt gtcagaaaac aaccaagtac
      721 agagtggggt tgctttccac catggcaagt tcatcatgag tctccggaaa gcagctatgg
      781 cagagcccaa tgtaaagttt atagaaggtg ttgtgcttcg gttactagag gaagatgatg
      841 ctgtgattgg tgtgcaatac aaggacaagg agactgggga caccaaggag ctccatgccc
      901 cgctcactgt tgttgccgac gggctcttct ccaagttcag gaagaacctc atctccaata
      961 aagtctctgt ttcctcccac ttcgttggct tcattatgaa ggatgcacca cagtttaaag
     1021 ccaatttcgc ggagcttgtt ctggtcgatc ccagtccagt tctcatctac cagatttcac
     1081 ccagcgaaac tagggtgctt gtcgatattc gaggagaatt gccaagaaac ttaagagaat
     1141 acatgacaga acaaatttac ccacagatac ctgatcactt gaaggaatca tttctggagg
     1201 cctgtcagaa tgctcgtctg cggaccatgc cagcaagctt ccttcctcct tcctcagtga
     1261 acaaacgagg cgtcctactt ctgggagatg cgtataacct gaggcatcct cttactggtg
     1321 gaggaatgac agtcgcttta aaagatataa aaatatggag acaactgtta aaagatattc
     1381 ctgaccttta tgatgatgcc gctattttcc aggccaaaaa gtcattcttt tggtcaagaa
     1441 aaaggagcca ttcctttgtt gtgaacgtgc tggctcaggc gttgtatgaa ctattttctg
     1501 ctacagatga ttccttgcgt cagctccgaa aagcttgctt tctttatttt aaacttggtg
     1561 gagaatgttt gactggtcct gttgggttgc tttctatatt gtctcctgac cctctcctat
     1621 tgattcgaca cttcttctcc gttgcagtct atgccacgta tttctgcttc aagtcagagc
     1681 catgggctac aaaacctcgg gctcttttca gtagtggtgc tatactgtac aaagcgtgct
     1741 ctataatatt tcctctcatc tattcagaaa tgaagtatct ggttcactga aagggaaacc
     1801 acatggaagt cattgggact tgaggaggat gtacatagcc taggactgtg tgtgtgactt
     1861 ctgcagtggt gactcaagga ccaagcttgt gtataagcat gtaaatatat gccttaattc
     1921 acaattgaga atgaagggtc aataagctag atgtttaaaa gaatgattgt ttccacaaat
     1981 taatgttaac actcaagtgt ttgatttcag tattggggtg attcattggg acatgccaat
     2041 aaaatgaaga gtgaactcta aaaaaaaaaa aaaaaaaaaa aaaaa
//
