LOCUS       NM_001037097            6507 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus serine palmitoyltransferase, long chain base
            subunit 2 (Sptlc2), mRNA.
ACCESSION   NM_001037097 XM_039112649 XM_345708
VERSION     NM_001037097.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Lu,G., Wu,R., Wang,B., Li,L., Li,Y., Li,X., He,H., Wang,X. and
            Kuang,L.
  TITLE     SPTLC2 ameliorates chondrocyte dysfunction and extracellular matrix
            metabolism disturbance in vitro and in vivo in osteoarthritis
  JOURNAL   Exp Cell Res 425 (1), 113524 (2023)
   PUBMED   36828166
  REMARK    GeneRIF: SPTLC2 ameliorates chondrocyte dysfunction and
            extracellular matrix metabolism disturbance in vitro and in vivo in
            osteoarthritis.
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Thamizhoviya,G. and Vanisree,A.J.
  TITLE     Enriched Environment Enhances the Myelin Regulatory Factor by mTOR
            Signaling and Protects the Myelin Membrane Against Oxidative Damage
            in Rats Exposed to Chronic Immobilization Stress
  JOURNAL   Neurochem Res 46 (12), 3314-3324 (2021)
   PUBMED   34449011
REFERENCE   3  (bases 1 to 6507)
  AUTHORS   Lee,S.Y., Lee,H.Y., Song,J.H., Kim,G.T., Jeon,S., Song,Y.J.,
            Lee,J.S., Hur,J.H., Oh,H.H., Park,S.Y., Shim,S.M., Yoo,H.J.,
            Lee,B.C., Jiang,X.C., Choi,C.S. and Park,T.S.
  TITLE     Adipocyte-Specific Deficiency of De Novo Sphingolipid Biosynthesis
            Leads to Lipodystrophy and Insulin Resistance
  JOURNAL   Diabetes 66 (10), 2596-2609 (2017)
   PUBMED   28698261
REFERENCE   4  (bases 1 to 6507)
  AUTHORS   Chaurasia,B., Kaddai,V.A., Lancaster,G.I., Henstridge,D.C.,
            Sriram,S., Galam,D.L., Gopalan,V., Prakash,K.N., Velan,S.S.,
            Bulchand,S., Tsong,T.J., Wang,M., Siddique,M.M., Yuguang,G.,
            Sigmundsson,K., Mellet,N.A., Weir,J.M., Meikle,P.J., Bin M
            Yassin,M.S., Shabbir,A., Shayman,J.A., Hirabayashi,Y., Shiow,S.T.,
            Sugii,S. and Summers,S.A.
  TITLE     Adipocyte Ceramides Regulate Subcutaneous Adipose Browning,
            Inflammation, and Metabolism
  JOURNAL   Cell Metab 24 (6), 820-834 (2016)
   PUBMED   27818258
REFERENCE   5  (bases 1 to 6507)
  AUTHORS   Suriyanarayanan,S., Auranen,M., Toppila,J., Paetau,A.,
            Shcherbii,M., Palin,E., Wei,Y., Lohioja,T., Schlotter-Weigel,B.,
            Schon,U., Abicht,A., Rautenstrauss,B., Tyynismaa,H., Walter,M.C.,
            Hornemann,T. and Ylikallio,E.
  TITLE     The Variant p.(Arg183Trp) in SPTLC2 Causes Late-Onset Hereditary
            Sensory Neuropathy
  JOURNAL   Neuromolecular Med 18 (1), 81-90 (2016)
   PUBMED   26573920
REFERENCE   6  (bases 1 to 6507)
  AUTHORS   Rotthier,A., Auer-Grumbach,M., Janssens,K., Baets,J., Penno,A.,
            Almeida-Souza,L., Van Hoof,K., Jacobs,A., De Vriendt,E.,
            Schlotter-Weigel,B., Loscher,W., Vondracek,P., Seeman,P., De
            Jonghe,P., Van Dijck,P., Jordanova,A., Hornemann,T. and
            Timmerman,V.
  TITLE     Mutations in the SPTLC2 subunit of serine palmitoyltransferase
            cause hereditary sensory and autonomic neuropathy type I
  JOURNAL   Am J Hum Genet 87 (4), 513-522 (2010)
   PUBMED   20920666
REFERENCE   7  (bases 1 to 6507)
  AUTHORS   Han,G., Gupta,S.D., Gable,K., Niranjanakumari,S., Moitra,P.,
            Eichler,F., Brown,R.H. Jr., Harmon,J.M. and Dunn,T.M.
  TITLE     Identification of small subunits of mammalian serine
            palmitoyltransferase that confer distinct acyl-CoA substrate
            specificities
  JOURNAL   Proc Natl Acad Sci U S A 106 (20), 8186-8191 (2009)
   PUBMED   19416851
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 2009 Jun 16;106(24):9931]
REFERENCE   8  (bases 1 to 6507)
  AUTHORS   Hojjati,M.R., Li,Z. and Jiang,X.C.
  TITLE     Serine palmitoyl-CoA transferase (SPT) deficiency and sphingolipid
            levels in mice
  JOURNAL   Biochim Biophys Acta 1737 (1), 44-51 (2005)
   PUBMED   16216550
REFERENCE   9  (bases 1 to 6507)
  AUTHORS   Uhlinger,D.J., Carton,J.M., Argentieri,D.C., Damiano,B.P. and
            Dandrea,M.R.
  TITLE     Increased expression of serine palmitoyltransferase (SPT) in
            balloon-injured rat carotid artery
  JOURNAL   Thromb Haemost 86 (5), 1320-1326 (2001)
   PUBMED   11816724
  REMARK    GeneRIF: marked upregulation of expression of both SPT subunits in
            actively proliferating cells in balloon-injured vessels(serine
            palmitoyltransferase 1)(serine palmitoyltransferase 2)
REFERENCE   10 (bases 1 to 6507)
  AUTHORS   Weiss,B. and Stoffel,W.
  TITLE     Human and murine serine-palmitoyl-CoA transferase--cloning,
            expression and characterization of the key enzyme in sphingolipid
            synthesis
  JOURNAL   Eur J Biochem 249 (1), 239-247 (1997)
   PUBMED   9363775
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000166.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001037097.3.
            
            On or before Jun 3, 2021 this sequence version replaced
            XM_039112649.1, NM_001037097.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-226               JACYVU010000166.1  7030772-7030997     c
            227-421             JACYVU010000166.1  7009141-7009335     c
            422-576             JACYVU010000166.1  6995048-6995202     c
            577-725             JACYVU010000166.1  6993195-6993343     c
            726-850             JACYVU010000166.1  6989941-6990065     c
            851-944             JACYVU010000166.1  6984647-6984740     c
            945-1050            JACYVU010000166.1  6981659-6981764     c
            1051-1270           JACYVU010000166.1  6980461-6980680     c
            1271-1397           JACYVU010000166.1  6976479-6976605     c
            1398-1533           JACYVU010000166.1  6957249-6957384     c
            1534-1663           JACYVU010000166.1  6956086-6956215     c
            1664-6507           JACYVU010000166.1  6948147-6952990     c
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q31"
     gene            1..6507
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /note="serine palmitoyltransferase, long chain base
                     subunit 2"
                     /db_xref="GeneID:366697"
                     /db_xref="RGD:1305447"
     exon            1..226
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..1783
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /EC_number="2.3.1.50"
                     /note="protein-O-mannosyltransferase 2;
                     serine-palmitoyl-CoA transferase 2; long chain base
                     biosynthesis protein 2"
                     /codon_start=1
                     /product="serine palmitoyltransferase 2"
                     /protein_id="NP_001032174.1"
                     /db_xref="GeneID:366697"
                     /db_xref="RGD:1305447"
                     /translation="MRPEPGGCCCRRPMRANGCVKNGEVRNGYLRSSTATIAAAGQIH
                     HITENGGLYKRPFNEAFEETPMLVAVLTYVGYGVLTLFGYLRDFLRHWRIEKCHHATE
                     REEQKDFVSLYQDFENFYTRNLYMRIRDNWNRPICSVPGAKVDIMERQSHDYNWSFKY
                     TGNIIKGVINMGSYNYLGFARNTGSCQEAAAEVLKTYGAGVCSTRQEIGNLDKHEELE
                     KLVARFLGVEAALTYGMGFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGATIR
                     IFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKK
                     YKAYLYLDEAHSIGALGPSGRGVVDYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKEL
                     IDYLRTHSHSAVYATSMSPPVMEQIITSMKCIMGQDGTSLGKECIQQLAENTRYFRRR
                     LKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARF
                     CLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRPLPLLDRPFDETTYEETED"
     exon            227..421
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            422..576
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            577..725
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            726..850
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            851..944
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1050
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1270
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1397
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1533
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1663
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..6507
                     /gene="Sptlc2"
                     /gene_synonym="LCB 2; Pomt2; SPT 2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagccatttc cggtttcgga gaggtgggtg cggtgcggag cgggagttgg cgccgccgcc
       61 gcctccagag cctgtcttgc tgccgggtgc tgccgggagg atgcggccgg agccaggagg
      121 ctgctgctgc cgccgcccga tgcgggcgaa cggctgcgtg aaaaacgggg aagtgaggaa
      181 cgggtacttg aggagcagca ccgccaccat cgcggctgcg ggccagattc atcatattac
      241 agaaaatgga ggactgtaca aaagaccgtt taatgaagct tttgaagaaa cacccatgct
      301 agttgctgtg ctcacatacg tgggctatgg tgtactcacc ctctttggat atcttcgaga
      361 tttcttgagg cattggagaa ttgaaaagtg ccaccatgca acagaaagag aagaacaaaa
      421 ggactttgtg tccttgtatc aggattttga aaacttctat acaaggaacc tctacatgag
      481 gatcagagac aactggaatc gacccatctg tagtgtgcct ggagccaagg tggatatcat
      541 ggagagacag tctcatgact acaactggtc attcaagtac acagggaata taattaaagg
      601 tgtaataaac atgggttcct acaactatct tggatttgcg aggaacactg gatcatgtca
      661 ggaagcagct gctgaagttc tcaagacata tggagcaggg gtgtgcagca ctcgccagga
      721 aattgggaac ctggacaagc acgaggaact agagaaactg gtagcaaggt tcttgggtgt
      781 ggaagctgct ttgacctatg gcatgggatt tgcgacaaat tcaatgaaca ttcctgctct
      841 tgttggcaaa ggttgcctga ttctgagtga tgagctgaac catgcatcct tggttctagg
      901 agccagactg tcgggagcaa ccattcgaat cttcaaacac aacaatatgc aaagcttgga
      961 gaagctttta aaagatgcca ttgtttatgg tcagcctcgg acaagaaggc cctggaagaa
     1021 aatcctaatc ctcgtggaag gcatatatag catggaaggg tctattgttc gccttcctga
     1081 agtgattgct ctcaagaaga aatacaaggc atacttgtat ctggatgagg ctcacagcat
     1141 tggggctctt ggcccttcag ggcgtggtgt agtagactac tttggcctgg atcctgagga
     1201 tgtagatgtt atgatgggaa cattcacaaa gagcttcggt gcttcaggag gatacattgg
     1261 cggcaagaag gagctgatag actacctacg cacacattct cacagtgctg tgtacgccac
     1321 gtcaatgtca ccacctgtga tggagcagat catcacctcc atgaagtgca tcatggggca
     1381 ggatggcacc agtcttggca aagaatgtat ccagcagttg gctgagaaca ccaggtattt
     1441 caggagacgc ctgaaggaaa tgggtttcat catctatggc aatgaagact ccccggtggt
     1501 gcctttgatg ctctacatgc cggccaaaat tggcgccttt gggagagaga tgctgaagcg
     1561 gaacattggt gtagttgtgg tgggatttcc tgctacccca atcattgagt ccagagccag
     1621 attttgcctg tcagcggctc ataccaaaga aatacttgac acggctttga aggagataga
     1681 tgaagttggg gatctgctgc aactgaagta ctcccgccac cggccgctgc ctctgctgga
     1741 caggcccttt gacgagacta cctatgagga gacagaagac tgagcctttc tggtgctccc
     1801 tagaggggga tgcttcctcc caggacagtg tgtggccttt ctgagccaat cccaggagcc
     1861 acacttcagt gaccacttca tgtgaaagac atttcccaag ttactgaaga tggccacctc
     1921 cactccaaat ggcattttgt aaatagtaaa aaaacaaact gcttcacatt cttcctttgt
     1981 ttgctaagtc tcacacacat acacacacac atatatatat gattactttg tccattaaaa
     2041 atgttttatt ttataactat tctacttgtg aaatcacgct gacccttgcc tgtggcttct
     2101 ggaacaggca ttccccactg cagtcacctc ataggcttgg atgcatcaag acctacttca
     2161 ggccctggct ctccagcctt gagactcact ggccctctcc ctagggagca agtgccttct
     2221 gctgacttcc cgcccgggtc tcagaggttg tcccccttgt aatccctttt taaccagtcg
     2281 agttaaccaa ccaaagcatt ctgtctttag tcttaaggta aacgtaacca agacccaatg
     2341 gcttatgtga ctcctcctgg ccaaggactt cctaaaactg gtctcttttt gttgttgttc
     2401 tgttcatcaa ataatttatt cactatcaga tctttcttca tatgatctct gaagatgtat
     2461 ctcatgattt agttctccct gagatctaac cctaatgctt cccacctcca aagttacaac
     2521 cccctctggc tcattcaggg tccagattaa gatttgacca gtaaaggcaa gtgttggtca
     2581 gtcctttgct catgtcccac ttcaccatta gtggggtaag ctttctgtgt cactgaccca
     2641 cttgaacaat gctcttccaa aggccagctc ttctactcct cctatgctaa caagaattta
     2701 cccagcatct gggctggctt ctcttgtgct tccattgtat ttcccccctt tgaggaattc
     2761 gtcaaatacc tctttcttag actggaatag cctgttttcc tgtctccact gggtgagggg
     2821 cttcatccag agctttgtgc tgcttgacca aagaacacat ccgaggagca ccgttttgaa
     2881 gttaattttt tttcttttga ccaaagcata attgaagaga acttttgact ttattgtact
     2941 gaagatgaat ttgtctttta gtactgatca agtgttataa ggaaggattg agaacagaat
     3001 gaggaaaaac tgtttagaag tcattttgta tgtcattctt taaagttatt attattactg
     3061 ataaagatgc tcaaaccaag ttcttctggg gaccgtcagc tgcaggtaca tggaagagaa
     3121 gcagtatcat ttgtgaagct gttttcatgc cttggatctt tggccctctt ggcatctgtg
     3181 agctttcagc ccttgagata ttgtaaggtt gtatgtggag gttttaaaga atagacatgg
     3241 gtgaacaggc tgggtgtggt aacctttgcc tttaatccca gatctcagaa ggcagaggca
     3301 agctaatctc ctgccggttt gaggtcagcc tggtatacat agagttccgg gatagcctgg
     3361 gttgcatagt gaaacctgga gagagagaga gagagagaga gagagagaga gagagagaga
     3421 gagagagaga gagagagaga gagagagaga gagaacacac accatagaaa gaagcaagaa
     3481 cttcatgtat aaaaagattc cataactaaa tcctataaag ctcaaacacc ttcctccctt
     3541 cccctgtaga agcccacggg aataccaagt attttgcaca gcactatttg atagtgcagc
     3601 atagggcttg caaaagtaac aaaccttaag caatgaacat gcccactagc ctctgagctt
     3661 acatgaaatc acttcaaatt aaaatgctac tccaggcatt taaatatttc tgtatgtatc
     3721 ttggttggtg caaagtttat ttcgggctaa atgtaattaa gttaaagttt tttattgtta
     3781 ttgtttgttt ggatgttttg ttgtgtcttt gttttgagga gacaaagccc ctgtgatctg
     3841 cccatgatct ctattttcta gaggtcagaa tggcttacac actttatatg tggctggtta
     3901 tttgtaaatc tgtaatagac atctccggcc cagtcatgtc atgggtgatt atccagactt
     3961 ctggggagac agagtgtata ctggtctgac gtttgagagt ggggagatgt ctgggcctga
     4021 gacagattca ggcaatcaca ggtcagtcat catcaccaga gccgaccgta ctgtgcctca
     4081 tgcctttcat ttatcattgc cctcacacgt ctcgtgttta taagacccat ttcacagcgc
     4141 ccatcaggaa ccagaaggta atcagaatca tgcagaagat ttggtaggca aaatatataa
     4201 aatagaggta aataaatttt aaattttttt gggaaaaaca gtagccagcc ctcttcgacc
     4261 tttcattcac tgtcttcaac tcttctgacc caggcaggct ttcaaaatga gcttccttgt
     4321 ggcctcactt ctggcctaca ccctctcata ggctgaaggg tagagcatcc tcagagatct
     4381 gcctctggtc ccttactcct cattctgtat cttgttggtg gttgggagtc cccctccaga
     4441 gaagactgac aagtgctggt gcagcatctg gggagctgtc gggcagagag cctgctcttt
     4501 gcttgtacaa ccctgcacct cagcgtttct tcccttattc attaagggca tccatatctg
     4561 agtctctgtt gactggcagg gtatggccac atcacctggt gtgtaatgta gctggcaaaa
     4621 atcaggataa acctctgctt agatgtcttg acccgttgga caaagagggg aatgaaacct
     4681 gctaccacct gctttcagaa ttgatgaatc tacctaattg cagtattttg acaaaaccca
     4741 taggaatata aataaattgt tgctaggggt cagatatggt acacacctgt aatcccaata
     4801 ctcagaccac agaagcaaaa ggatcttcta gaattctaga ttatcctgtg ctaccaagtg
     4861 agactccaga aagaacaaaa aaggtggatt gctatgactt aatgcccaag cactgctctc
     4921 ccagggttct agctaagaca cagatgggtg ttcctgtctc tttgcttcca gtatgaggtt
     4981 agttaggaaa agaagagcag tgtctttcct ctccgcctgt gctgtgctga tcaggagctg
     5041 ttgagtattt ctcacaggta aaatcacctt agccaggacc catgaagaga gggtttatga
     5101 aagtcaagat tctgcttggc agtttgtcaa agaatctata gacccccacc tcttccctca
     5161 gttcaagaac ctatggatta gctattacct gcttaacaat aaactcagtg cccagcatgc
     5221 gtcctaatcc actaagccag tcaatcaaaa taccttccat gtggtgaaaa gcatgtcctc
     5281 cctcccctca gccttatcca catgagatgt gcactctggc ttccagccag ctcagagggt
     5341 agagagaaat gccagggctc tgtgatcccc tatcttgtct acaaaaaact atttctgttc
     5401 tagtttcccg tgacttgttt tccaagatgc cttttgccat agattgaaag gtttctagga
     5461 aggaaacaag cacggcaacc cgcataagcc ctaaggatga gcactttcac ctctgcagag
     5521 gctgaagatt cacatccaca gttcacctta ggagcagctt catcgggggc gcttcgagca
     5581 aggttttgtt tactatggta tggccttaaa ctgttagcgt tttctagtgt gatgaattat
     5641 aataagctga ccctgaagaa tgctgtgcct cttgcctcgt gtcacagttt ttaaagaagc
     5701 aatgcctaag gctttgttct ttgggagcag ttgttggaga cggaatgaaa ggtgcttaca
     5761 agaacagcac cctgatgtgc tcagagtctg ttccttgtgc cctctgcact ctttctatgg
     5821 gcaaggaacc ccagctaact caaaggccag cacaaagacc aggtcattcc tgattgatca
     5881 gcataggcct tccaactgag tagaggatgc accaccaagt gtggtggagt taccttcccc
     5941 atgacattgt acgtcaggat tagttggcca gtaaactaag gaattgaaag cttccttact
     6001 ggttcattct tcacaggaca tggagacctc ctgttagttt ggcctgcatg cccacagtct
     6061 tgatgatgct tctctgtagg cagctaacaa taagggatgg gggaaagaga agagatcagg
     6121 tgtccatggg agagttgaat tagggtggtc ttggtgatcc atgtttactg aaggctggag
     6181 tctgcaaggt gtactatgac actaaccctt ttccttgttg taatgttatt atcatctggg
     6241 aatgtgagat ttttaatttt agtaatgtct tacaaataat cactcttctg tagcctccta
     6301 ccttcttgcc agatgtcttt tttatcatca tcatcattat tattattatt agtattatta
     6361 tttcacttta tattggatga ctgtgaagct atacatagta ggttgtggag gatatcaatt
     6421 ttcagagtgc atgttaagaa ttaaagaaga cctacttgag atgaatcatc ccatattgtt
     6481 ttgccctaat aaaatattca gaaatat
//
