LOCUS       NM_019167               8186 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Rattus norvegicus spectrin, beta, non-erythrocytic 2 (Sptbn2),
            mRNA.
ACCESSION   NM_019167
VERSION     NM_019167.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Efimova,N., Korobova,F., Stankewich,M.C., Moberly,A.H., Stolz,D.B.,
            Wang,J., Kashina,A., Ma,M. and Svitkina,T.
  TITLE     betaIII Spectrin Is Necessary for Formation of the Constricted Neck
            of Dendritic Spines and Regulation of Synaptic Activity in Neurons
  JOURNAL   J Neurosci 37 (27), 6442-6459 (2017)
   PUBMED   28576936
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Kobeissy,F.H., Liu,M.C., Yang,Z., Zhang,Z., Zheng,W.,
            Glushakova,O., Mondello,S., Anagli,J., Hayes,R.L. and Wang,K.K.
  TITLE     Degradation of betaII-Spectrin Protein by Calpain-2 and Caspase-3
            Under Neurotoxic and Traumatic Brain Injury Conditions
  JOURNAL   Mol Neurobiol 52 (1), 696-709 (2015)
   PUBMED   25270371
  REMARK    GeneRIF: Results revealed that betaII-spectrin was degraded into
            major fragments of 110/85 kDa by calpain-2 activation and 108/80
            kDa by caspase-3 activation.
            Erratum:[Mol Neurobiol. 2018 Jan;55(1):898-900. doi:
            10.1007/s12035-017-0764-3.. PMID: 28975597]
REFERENCE   3  (bases 1 to 8186)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8186)
  AUTHORS   Salcedo-Sicilia,L., Granell,S., Jovic,M., Sicart,A., Mato,E.,
            Johannes,L., Balla,T. and Egea,G.
  TITLE     betaIII spectrin regulates the structural integrity and the
            secretory protein transport of the Golgi complex
  JOURNAL   J Biol Chem 288 (4), 2157-2166 (2013)
   PUBMED   23233669
  REMARK    GeneRIF: betaIII spectrin regulates the structural integrity and
            the secretory protein transport of the Golgi complex
REFERENCE   5  (bases 1 to 8186)
  AUTHORS   Bohm,D., Keller,K., Pieter,J., Boehm,N., Wolters,D., Siggelkow,W.,
            Lebrecht,A., Schmidt,M., Kolbl,H., Pfeiffer,N. and Grus,F.H.
  TITLE     Comparison of tear protein levels in breast cancer patients and
            healthy controls using a de novo proteomic approach
  JOURNAL   Oncol Rep 28 (2), 429-438 (2012)
   PUBMED   22664934
REFERENCE   6  (bases 1 to 8186)
  AUTHORS   Page,L.J., Sowerby,P.J., Lui,W.W. and Robinson,M.S.
  TITLE     Gamma-synergin: an EH domain-containing protein that interacts with
            gamma-adaptin
  JOURNAL   J Cell Biol 146 (5), 993-1004 (1999)
   PUBMED   10477754
REFERENCE   7  (bases 1 to 8186)
  AUTHORS   Stankewich,M.C., Tse,W.T., Peters,L.L., Ch'ng,Y., John,K.M.,
            Stabach,P.R., Devarajan,P., Morrow,J.S. and Lux,S.E.
  TITLE     A widely expressed betaIII spectrin associated with Golgi and
            cytoplasmic vesicles
  JOURNAL   Proc Natl Acad Sci U S A 95 (24), 14158-14163 (1998)
   PUBMED   9826670
REFERENCE   8  (bases 1 to 8186)
  AUTHORS   Sakaguchi,G., Orita,S., Naito,A., Maeda,M., Igarashi,H., Sasaki,T.
            and Takai,Y.
  TITLE     A novel brain-specific isoform of beta spectrin: isolation and its
            interaction with Munc13
  JOURNAL   Biochem Biophys Res Commun 248 (3), 846-851 (1998)
   PUBMED   9704016
REFERENCE   9  (bases 1 to 8186)
  AUTHORS   Ohara,O., Ohara,R., Yamakawa,H., Nakajima,D. and Nakayama,M.
  TITLE     Characterization of a new beta-spectrin gene which is predominantly
            expressed in brain
  JOURNAL   Brain Res Mol Brain Res 57 (2), 181-192 (1998)
   PUBMED   9675416
REFERENCE   10 (bases 1 to 8186)
  AUTHORS   Zagon,I.S., Higbee,R., Riederer,B.M. and Goodman,S.R.
  TITLE     Spectrin subtypes in mammalian brain: an immunoelectron microscopic
            study
  JOURNAL   J Neurosci 6 (10), 2977-2986 (1986)
   PUBMED   3531427
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Sep 2, 2015 this sequence version replaced NM_019167.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB001347.1, SRR26643298.6380.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-368               JAXUCZ010000001.1  211432373-211432740
            369-547             JAXUCZ010000001.1  211439497-211439675
            548-699             JAXUCZ010000001.1  211444708-211444859
            700-873             JAXUCZ010000001.1  211445225-211445398
            874-965             JAXUCZ010000001.1  211446310-211446401
            966-1046            JAXUCZ010000001.1  211446545-211446625
            1047-1162           JAXUCZ010000001.1  211447002-211447117
            1163-1275           JAXUCZ010000001.1  211449501-211449613
            1276-1463           JAXUCZ010000001.1  211449736-211449923
            1464-1581           JAXUCZ010000001.1  211451849-211451966
            1582-1740           JAXUCZ010000001.1  211452529-211452687
            1741-2043           JAXUCZ010000001.1  211452984-211453286
            2044-2197           JAXUCZ010000001.1  211454552-211454705
            2198-3068           JAXUCZ010000001.1  211454926-211455796
            3069-3206           JAXUCZ010000001.1  211457838-211457975
            3207-3963           JAXUCZ010000001.1  211458348-211459104
            3964-4166           JAXUCZ010000001.1  211459633-211459835
            4167-4257           JAXUCZ010000001.1  211460161-211460251
            4258-4404           JAXUCZ010000001.1  211460426-211460572
            4405-4668           JAXUCZ010000001.1  211462899-211463162
            4669-4893           JAXUCZ010000001.1  211464614-211464838
            4894-4983           JAXUCZ010000001.1  211465033-211465122
            4984-5244           JAXUCZ010000001.1  211465751-211466011
            5245-5375           JAXUCZ010000001.1  211466099-211466229
            5376-5580           JAXUCZ010000001.1  211466426-211466630
            5581-5955           JAXUCZ010000001.1  211467220-211467594
            5956-6200           JAXUCZ010000001.1  211468320-211468564
            6201-6339           JAXUCZ010000001.1  211468667-211468805
            6340-6424           JAXUCZ010000001.1  211469206-211469290
            6425-6621           JAXUCZ010000001.1  211469553-211469749
            6622-6764           JAXUCZ010000001.1  211470033-211470175
            6765-6840           JAXUCZ010000001.1  211470264-211470339
            6841-6891           JAXUCZ010000001.1  211470413-211470463
            6892-7112           JAXUCZ010000001.1  211470656-211470876
            7113-7286           JAXUCZ010000001.1  211471103-211471276
            7287-7329           JAXUCZ010000001.1  211471792-211471834
            7330-8186           JAXUCZ010000001.1  211472160-211473016
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     gene            1..8186
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /note="spectrin, beta, non-erythrocytic 2"
                     /db_xref="GeneID:29211"
                     /db_xref="RGD:3751"
     exon            1..368
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            369..547
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     CDS             391..7557
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /note="spectrin beta chain, brain 2; beta-III spectrin;
                     beta SpIII sigma 1; spectrin-like protein GTRAP41;
                     spectrin, non-erythroid beta chain 2; glutamate
                     transporter EAAT4-associated protein 41; beta-spectrin 3"
                     /codon_start=1
                     /product="spectrin beta chain, non-erythrocytic 2"
                     /protein_id="NP_062040.2"
                     /db_xref="GeneID:29211"
                     /db_xref="RGD:3751"
                     /translation="MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFE
                     RSRIKALADEREAVQKKTFTKWVNSHLARVTCRVGDLYSDLRDGRNLLRLLEVLSGET
                     LPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLVWTIILRF
                     QIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKH
                     RPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATY
                     YHYFSKMKALAVEGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLA
                     NSLSGVQNQLQSFNSYRTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLI
                     SDINKAWERLEKAEHERELALRTELIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQ
                     DNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAELAAEHYHDIKRIAARQNN
                     VARLWDFLREMVAARRERLLLNLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGV
                     EDLLQLHELVEADIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYE
                     ALCELAATRRARLEESRRLWRFLWEVGEAEAWVREQQHLLASAETGRDLTGVLRLLNK
                     HTALRGEMSGRLGPLKLTLEQGQQLVAEGHPGANQASTRAAELQAQWERLEALAEERA
                     QRLAQAASLYQFQADANDMEAWLVDALRLVSSPEVGHDEFSTQALARQHRALEEEIRA
                     HRPTLDALREQAAALPPALSHTPEVQGRVPTLEQHYEELQARAGERARALEAALAFYT
                     MLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNALAARVTAVSDI
                     AEQLLKASPPGKDRIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQA
                     WMREKTKVIESTQDLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHP
                     AQAPAINTRLGEVQTGWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAV
                     ASEEGPATLPEAEALLAQHAALRGEVERAQSEYSRLRTLGEEVTRDQADPQCLFLRQR
                     LEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTL
                     QAADAAIKKLEDFMSTMDANGERIRGLLEAGRQLVSKGNIHAEKIQEKADSIEKRHRK
                     NQEAVQQLLGRLRDNREQQHFLQDCQELKLWIDEKMLTAQDVSYDEARNLHTKWQKHQ
                     AFMAELAANKDWLDKVDKEGRELTLEKPELKVLVSEKLEDLHRRWDELETTTQAKARS
                     LFDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVRE
                     KEVEAIQAQAKALAQEDQSAGEVERTSRAVEEKFRALCQPMKDRCRRLQASREQHQFH
                     RDVEDEILWVTERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGHEPRIADLKERQR
                     TLGTAAAGPELAELQEMWKRLSHELELRGKRLEEALRAQQFYRDAAEAEAWMGEQELH
                     MMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPESTRLTIR
                     QAQVDKLYAGLKELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDY
                     EHVTMLRDKFREFSRDTSTIGQERVDSANALANGLIAGGHAARATVAEWKDSLNEAWA
                     DLLELLDTRGQVLAAAYELQRFLHGARQALARVQHKQQQLPDGTGRDLNAAEALQRRH
                     CAYEHDIQALSTQVQQVQDDGLRLQKAYAGDKAEEIGRHMQAVAEAWAQLQGSSAARR
                     QLLLDTTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIKNQQGIKAEIEA
                     RADRFSACIDMGQELLARNHYAAEEISEKLSQLQSRRQETAEKWQEKMDWLQLVLEVL
                     VFGRDAGMAEAWLCSQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFSALEK
                     LTALEERENEQKRKREEEERRKQPPTSEPMASQPEGSLVDGQRVLDTAWDGTQSKLPP
                     STQAPSINGVCTDTESSQPLLEQQRLEQSNVPEGPGSGTGDESSGPRGERQTLPRGPA
                     PSPMPQSRSSESAHVATLPARGAELSAQEQMEGTLCRKQEMEAFNKKAANRSWQNVYC
                     VLRRGSLGFYKDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEY
                     LFQAKDEAEMSSWLRVVNAAIATASSASGEPEEPVVPSASRGLTRAMTMPPVSQPEGS
                     IVLRSKDGREREREKRFSFFKKNK"
     exon            548..699
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            700..873
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            874..965
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1046
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1162
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1275
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1463
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1581
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1740
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..2043
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2197
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            2198..3068
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            3069..3206
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            3207..3963
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            3964..4166
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            4167..4257
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            4258..4404
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            4405..4668
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            4669..4893
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            4894..4983
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            4984..5244
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            5245..5375
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            5376..5580
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            5581..5955
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            5956..6200
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            6201..6339
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            6340..6424
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            6425..6621
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            6622..6764
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            6765..6840
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            6841..6891
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            6892..7112
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            7113..7286
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            7287..7329
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
     exon            7330..8186
                     /gene="Sptbn2"
                     /gene_synonym="Spnb3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccattaaacc gtagaccact cagctgctaa taaaccagga agaaacctag ccgtgccagg
       61 actagaaatg tccatccctg cccccaaccc ccttaaggca gtgactaggc cagaattctc
      121 ctggttgata aatgagagga gctgaggagc cgctgtgatg acggttccga gaatctgatt
      181 tgtgagaagg cgaatctcca ggagcaccat ggagatgctt ttggaaagcg ggccagccta
      241 cctgccggaa ggaggacgag agaaaggggg aaaggcagag agaaacgctt aagcgagatg
      301 gcctggaggt gcccaaaagg ggacatttga aaaggaaacg gaggccgcat ctcggggaaa
      361 cctcggagga acaggaagcc acccaccacc atgagcagca ccctgtcacc cactgacttc
      421 gacagcttgg agatccaggg ccagtacagt gacatcaaca accgctggga cctgcccgac
      481 tcagattggg acaatgacag cagttcagcc cgcctctttg agaggtccag aattaaggcc
      541 ctggcagatg agcgagaagc cgtgcagaag aaaaccttca ccaagtgggt gaactcccac
      601 ctggcccggg tgacatgccg ggtgggagac ctgtacagcg acctgcggga cgggcgcaac
      661 ctcctgaggc tcctggaggt gctctcggga gagaccctgc caaaacccac caagggccgg
      721 atgcggattc actgcctgga gaatgtcgac aaagcactgc agttcctgaa ggagcagaag
      781 gtgcacctgg aaaacatggg ctcccacgac attgtggatg ggaaccaccg tctgaccctt
      841 gggctagtgt ggaccatcat cctccgattt cagatccaag acatcagtgt ggagacagaa
      901 gacaacaagg agaagaagtc agccaaggat gccctgctgc tgtggtgcca gatgaagact
      961 gcagggtatc ccaatgtcaa tgtgcacaac tttaccacca gttggagaga tgggctggcc
     1021 tttaatgcca ttgtgcacaa acaccggcca gacctgttgg attttgagtc cctgaagaag
     1081 tgtaacgcac actacaatct gcagaatgct ttcaatctgg ctgaaaagga acttggcctg
     1141 acgaagctcc tggatcctga agatgtgaac gtagaccaac ccgatgagaa gtccatcatc
     1201 acctacgtgg ccacttacta ccactacttc tcgaagatga aggccctggc tgtggaaggc
     1261 aaaaggattg gcaaggtcct ggaccatgcc atggaggcag aacacctggt agagaaatat
     1321 gagtccctgg cctctgaact gctgcagtgg atcgagcaaa cgattgtgac cctcaatgac
     1381 cgacagctgg ccaactccct gagtggcgtc cagaaccagc tgcagtcttt caattcctac
     1441 cgcacggtgg agaagccacc caagttcaca gagaaaggga acttggaggt gttgctcttc
     1501 accatccaga gtaagctgcg ggccaacaac cagaaagtct acacaccacg cgaaggccgg
     1561 ctcatctcgg acatcaacaa ggcctgggag cggctagaga aagccgaaca tgagcgagag
     1621 ctggccctgc gcacggagct gatccgccag gagaagctgg agcaactggc tgctcgcttc
     1681 gaccgcaagg ctgccatgcg ggagacctgg ctcagtgaga accagcgcct cgtctcccag
     1741 gacaactttg gcctggagct ggcagcagtg gaggcagcag tgcggaagca tgaagccatt
     1801 gagacagaca ttgtggccta cagcggccgg gtgcaagcgg tggacgccgt agccgcagaa
     1861 ctggccgctg agcactacca tgacattaag cgcattgcgg cgcggcagaa caacgtggcc
     1921 cggctctggg acttcttacg agagatggtg gccgcccgcc gtgagcggct ccttctcaac
     1981 ctggagctgc agaaggtgtt tcaggacctg ctctacctca tggactggat ggcagagatg
     2041 aagggccggc tgcagtctca ggacctaggc aagcatctgg ctggagtgga agatctactg
     2101 caactacacg aactggtgga ggcggacatt gcagttcagg ctgagagggt gcgagcggtc
     2161 agcgcctctg ccctgcgctt ctgcgacccc gggaaagagt atagaccatg tgacccacag
     2221 ctagtgtcag agagggtagc cactctggag cagagctatg aggccctgtg cgaattggca
     2281 gcaactcgaa gggcccgact ggaagagtcc cgtcgtctct ggaggttcct ctgggaagtg
     2341 ggtgaggccg aggcctgggt tcgggagcag cagcacctcc tggcctcagc tgagacaggc
     2401 cgggacctga ctggtgtcct ccgcctgctc aataagcaca cagccctacg gggtgagatg
     2461 agtggccgcc tggggcccct gaagctcacc ctggaacaag gtcagcagtt agttgccgag
     2521 ggccaccctg gagctaacca agcctcaacc cgtgcagcag agctccaggc ccagtgggag
     2581 cgactagaag ccctggccga ggagcgagcc cagcggctag cacaggctgc cagcctctac
     2641 cagttccagg cagatgccaa tgacatggag gcttggttgg tggacgcact acgcctggta
     2701 tctagccctg aggtagggca cgatgagttc tccacgcagg ccctggccag gcagcacagg
     2761 gcccttgagg aggagatccg agcccaccgg cctacactgg atgccttgag ggagcaggct
     2821 gcagccctgc cacctgcact gagccacaca cctgaggtac agggcagggt gcccactctg
     2881 gagcagcact atgaggagct gcaggcccgg gcaggtgagc gtgcacgagc cctggaagca
     2941 gccctggcgt tctataccat gctcagcgag gccggggctt gtgggctctg ggtagaggag
     3001 aaggagcagt ggctcaacgg gctggcccta cctgagcgcc tggaggacct ggaggtggtc
     3061 caacagaggt ttgagacctt agagcccgaa atgaacgccc tggctgcacg ggttactgct
     3121 gtcagtgaca tagctgagca gttgctgaag gccagtccac caggcaagga ccgcatcatt
     3181 ggcacccagg agcagctcaa ccaaaggtgg cagcagttca ggtccctggc agatggcaaa
     3241 aaggcagctc tgacatcagc cctgagcatc cagaattacc acctagagtg cacagagacc
     3301 caggcctgga tgagagaaaa gaccaaggtc attgagtcta cccaggacct aggcaatgat
     3361 ctagctggtg tgctggccct gcagcggaag ctggcaggca ctgagagaga tctggaagcc
     3421 atctctgccc gggtgggtga gctgacccaa gaggcaaatg ctttggctgc tgggcaccca
     3481 gcccaagccc ctgccatcaa cacacggctt ggagaggttc aaactggatg ggaggatctt
     3541 cgggcaacca tgaggcggag agaagagtcc ctgggtgagg ctcgacggct gcaagatttc
     3601 ctgcgcagct tggatgactt ccaggcctgg ctaggccgca cacagactgc tgtagcctct
     3661 gaggaaggac cagccaccct tccagaggca gaagccctct tagcccagca tgcagctctg
     3721 cggggagagg tggagagagc ccagagcgag tacagccgcc tcaggacctt gggcgaggag
     3781 gtgaccagag accaggctga tccccaatgc ctcttcctca gacagaggct ggaagccctt
     3841 ggaaccggct gggaggagct gggtcgcatg tgggagagcc ggcaaggccg cttggcccag
     3901 gcccatggct tccaggggtt tttgagagat gctcgccagg ctgagggagt tctcagcagc
     3961 caggaatatg ttctatctca cacggagatg ccagggacac tgcaggcggc ggatgcagcc
     4021 attaaaaagc tggaagactt catgagcacc atggacgcca atggagagcg catccgtgga
     4081 ctcctggagg ctggccgtca gctggtgtcc aagggcaata tccatgctga gaagatccaa
     4141 gagaaggcag actccatcga gaagaggcac agaaagaacc aggaggccgt gcagcagctt
     4201 ctaggccgcc ttcgggacaa ccgagagcag cagcacttct tgcaagactg tcaggagctg
     4261 aaactctgga ttgacgagaa gatgctgaca gctcaggatg tgtcctatga tgaggcacgc
     4321 aacctgcaca ccaagtggca aaaacaccag gcattcatgg ccgagctggc agccaacaag
     4381 gactggctgg acaaagtgga caaggaaggg cgggagctga ctcttgaaaa gccagaactc
     4441 aaagtcctag tgtcagagaa gctggaggac ctgcacaggc gctgggatga actggagact
     4501 accacccaag ccaaggcccg cagtcttttt gatgctaacc gggcagagct atttgcccaa
     4561 agctgttctg ccctggagag ctggctggag agcctgcagg cccagctgca ctcagatgac
     4621 tatggcaagg acctcaccag tgtcaacatt ctgctaaaga agcaacagat gctggaacga
     4681 gagatggctg tgagagagaa ggaggtagag gctatccagg cccaggcaaa agccctggcc
     4741 caggaagacc aaagtgcagg agaggtggaa aggacctcca gagctgtgga ggagaagttc
     4801 agggccttgt gtcagcccat gaaggaccgc tgccggcgcc tgcaagcctc ccgagagcag
     4861 caccagttcc accgggatgt ggaggatgag atactgtggg tgaccgagcg gcttcccatg
     4921 gccagctctc tggagcatgg caaggacttg cccagcgtcc agcttctcat gaagaaaaac
     4981 cagactctgc agaaggagat ccagggccat gagccccgga ttgcagacct caaagagaga
     5041 cagcgcactc tgggaacagc agcagcgggt ccagagctgg ctgagctcca ggaaatgtgg
     5101 aaacgcctga gccatgagct ggagcttcgg ggtaaacgac tggaggaggc ccttcgagcc
     5161 cagcaattct atcgtgacgc tgcagaggcc gaggcttgga tgggggagca ggagttacat
     5221 atgatgggcc aggaaaaggc caaggatgag ctgagcgccc aggcagaagt gaagaagcat
     5281 caggtactag aacaagccct tgctgactat gcccagacca tcaaacaact agcagccagc
     5341 agtcaagata tgattgacca tgaacatcca gagagcacaa ggttaacaat acgccaagcc
     5401 caggtggaca agctgtatgc cggcctaaag gagctggcag gagagcggcg tgagcgtctg
     5461 caggagcacc tcaggctgtg ccagctccgc agagagctgg atgacctgga gcagtggata
     5521 caggagcgag aagtcgtggc agcctcccat gaactgggcc aggactatga gcatgtgact
     5581 atgcttcggg acaaattccg agagttctcc agggacacca gcaccattgg ccaagagcgt
     5641 gtagacagtg ccaatgccct ggccaatggg ctcattgctg ggggccatgc tgcacgggcc
     5701 accgtggccg agtggaagga cagtctcaat gaggcctggg ctgacctgct ggagctgctg
     5761 gacacaagag gtcaggtgct ggctgctgct tatgagctgc agcgcttcct gcatggggcc
     5821 cgccaagccc tggcacgggt gcagcacaag cagcagcagc ttccagatgg gacgggccgc
     5881 gacctcaatg ctgctgaggc cctgcagcgc cggcactgcg cctatgagca cgacatccaa
     5941 gccctcagca ctcaggtcca gcaggttcag gacgatggcc tcaggctaca aaaggcctat
     6001 gctggagaca aggctgagga aattggccgt cacatgcagg cagtggctga ggcgtgggcc
     6061 cagctccagg gaagttctgc tgcccgtcgc cagctgttac tggataccac agacaaattc
     6121 cgattcttca aggctgtccg ggagttgatg ctgtggatgg atgggattaa cctgcagatg
     6181 gatgcccagg agaggccccg ggatgtgtcc tctgcagatt tagtcatcaa aaaccaacaa
     6241 ggcatcaaag cagagataga ggcaagagct gacaggttct ccgcctgcat tgacatgggg
     6301 caagagctgc tggcccggaa ccactatgcc gctgaggaga tctcagagaa gctgtctcag
     6361 ctacagtccc ggcgccagga gacagctgaa aagtggcagg agaagatgga ctggctacag
     6421 cttgttttgg aggtgcttgt gtttgggaga gatgcaggca tggcagaggc ctggctatgc
     6481 agtcaggagc cattggtgcg aagtgcagaa ctgggttgca ctgtggatga agtagagagc
     6541 ctcatcaagc ggcatgaagc cttccagaag tcagcagtgg cctgggagga gcgtttcagt
     6601 gccctggaga agctcactgc gctggaagag cgggagaatg agcagaaaag gaagagggag
     6661 gaggaggaac gaaggaaaca gccccctact tcagagccca tggctagtca accggaaggg
     6721 agtctggtag atggccagag agttcttgac actgcctggg atgggaccca gtcaaaattg
     6781 ccaccatcca cacaagcacc cagcattaat ggggtctgca cggacacgga gtcctcacag
     6841 cccctgttgg aacagcaaag acttgaacag agcaatgtcc cagaagggcc tggatctggc
     6901 acaggagacg agtccagcgg gccccgggga gagaggcaga ccctgccccg gggccctgct
     6961 ccgtctccaa tgccccagag cagatcgtct gagtcagctc atgttgccac cctgcccgca
     7021 cgaggtgctg agctctctgc tcaggaacag atggaaggga cgctgtgccg caaacaggag
     7081 atggaagcct tcaataagaa agctgccaac aggtcctggc agaatgtgta ctgtgtactt
     7141 cggcgtggaa gcctcggctt ttacaaggat gccagggcag ctagtgcagg agtgccatac
     7201 catggagaag tgcctgtcag tctggccagg gcccagggca gtgtggcctt tgattatcgg
     7261 aaacgcaaac atgtcttcaa gctgggcttg caggatggga aagagtatct attccaggcc
     7321 aaggatgagg cagagatgag ctcatggctg agagtggtga atgcagccat tgccactgcg
     7381 tcctcggcct ctggagagcc agaagagcca gtggtgccca gtgccagccg gggtctgacc
     7441 agggccatga ccatgccccc agtgtcacag cctgagggct ccatcgtgct tcgcagcaag
     7501 gatggcagag aaagagagcg agaaaaacga ttcagcttct ttaagaagaa caagtagttg
     7561 gggcaagact cccaggccag ctccctccct ctgttcagga aactgccagg gactgtcgac
     7621 agagaccacc ctcttggcag gacaactgcc tgctgctagg gtttgctgct aaggtcaacc
     7681 catcaccagg aactgtcact ggggacaaat gagcgttcct acaaccttct cttctgctat
     7741 ttaattcaac tggtggtggg acccaggcaa cccctaatac cactctttcc caccttgtca
     7801 cctcttccca agccttatac ctctatcctc cccaccatgg tgcagatagt gtcaccctcg
     7861 atgtgggcta tgtgggaacc attgtccctg gctcagggtc agtgtgccac cacagctagg
     7921 gtgtgccacc caccatgcag ttctttgcct ctgggatcag ccaggaccct cagagctgaa
     7981 gcaggcagga cctgagggca ggagcgccag atgatgaagt cggaggcgct gacaccttcc
     8041 ctgcctgtcc tgtctcaccc tctgactcaa ggggctgctt gtctccagcc actttaggtg
     8101 gctcccaagt gtgagttgtt caggagtagc cacatccttg agtctatcca aggaggtgat
     8161 taaacagctc ggcttctctc actgcc
//
