LOCUS       NM_001047094            4342 bp    mRNA    linear   ROD 17-FEB-2024
DEFINITION  Rattus norvegicus sprouty-related, EVH1 domain containing 2
            (Spred2), mRNA.
ACCESSION   NM_001047094 XM_001059428 XM_223647
VERSION     NM_001047094.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Liu,P., Zhuang,Y., Zhang,B., Huang,H., Wang,P., Wang,H., Cong,Y.,
            Qu,S., Zhang,K. and Wei,X.
  TITLE     miR-140-3p regulates the osteogenic differentiation ability of bone
            marrow mesenchymal stem cells by targeting spred2-mediated
            autophagy
  JOURNAL   Mol Cell Biochem 476 (12), 4277-4285 (2021)
   PUBMED   34406574
  REMARK    GeneRIF: miR-140-3p regulates the osteogenic differentiation
            ability of bone marrow mesenchymal stem cells by targeting
            spred2-mediated autophagy.
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Fan,P., Pu,D., Lv,X., Hu,N., Feng,X., Hao,Z., Sun,Y. and He,L.
  TITLE     Cav 1.3 damages the osteogenic differentiation in osteoporotic rats
            by negatively regulating Spred 2-mediated autophagy-induced cell
            senescence
  JOURNAL   J Cell Mol Med 24 (23), 13863-13875 (2020)
   PUBMED   33124763
  REMARK    GeneRIF: Cav 1.3 damages the osteogenic differentiation in
            osteoporotic rats by negatively regulating Spred 2-mediated
            autophagy-induced cell senescence.
REFERENCE   3  (bases 1 to 4342)
  AUTHORS   Friedmacher,F., Gosemann,J.H., Fujiwara,N., Takahashi,H.,
            Hofmann,A. and Puri,P.
  TITLE     Expression of Sproutys and SPREDs is decreased during lung
            branching morphogenesis in nitrofen-induced pulmonary hypoplasia
  JOURNAL   Pediatr Surg Int 29 (11), 1193-1198 (2013)
   PUBMED   23982388
  REMARK    GeneRIF: Decreased expression of Sproutys and SPREDs during the
            terminal pseudoglandular stage may disrupt lung branching
            morphogenesis by interfering with epithelial-mesenchymal
            interactions contributing to PH.
REFERENCE   4  (bases 1 to 4342)
  AUTHORS   Meng,S., Zhang,M., Pan,W., Li,Z., Anderson,D.H., Zhang,S., Ge,B.
            and Wang,C.
  TITLE     Tyrosines 303/343/353 within the Sprouty-related domain of Spred2
            are essential for its interaction with p85 and inhibitory effect on
            Ras/ERK activation
  JOURNAL   Int J Biochem Cell Biol 44 (5), 748-758 (2012)
   PUBMED   22305891
  REMARK    GeneRIF: Tyrosines 303/343/353 within the Sprouty-related domain of
            Spred2 are essential for its interaction with the p85 subunit of
            phosphatidylinositol 3-kinase.
REFERENCE   5  (bases 1 to 4342)
  AUTHORS   Li,D., Jackson,R.A., Yusoff,P. and Guy,G.R.
  TITLE     Direct association of Sprouty-related protein with an EVH1 domain
            (SPRED) 1 or SPRED2 with DYRK1A modifies substrate/kinase
            interactions
  JOURNAL   J Biol Chem 285 (46), 35374-35385 (2010)
   PUBMED   20736167
REFERENCE   6  (bases 1 to 4342)
  AUTHORS   Engelhardt,C.M., Bundschu,K., Messerschmitt,M., Renne,T.,
            Walter,U., Reinhard,M. and Schuh,K.
  TITLE     Expression and subcellular localization of Spred proteins in mouse
            and human tissues
  JOURNAL   Histochem Cell Biol 122 (6), 527-538 (2004)
   PUBMED   15580519
REFERENCE   7  (bases 1 to 4342)
  AUTHORS   Kato,R., Nonami,A., Taketomi,T., Wakioka,T., Kuroiwa,A., Matsuda,Y.
            and Yoshimura,A.
  TITLE     Molecular cloning of mammalian Spred-3 which suppresses tyrosine
            kinase-mediated Erk activation
  JOURNAL   Biochem Biophys Res Commun 302 (4), 767-772 (2003)
   PUBMED   12646235
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000254.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001047094.3.
            
            On Mar 5, 2021 this sequence version replaced NM_001047094.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132261,
                              SAMD00132262 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-510               JACYVU010000254.1  47850548-47851057
            511-688             JACYVU010000254.1  47927650-47927827
            689-857             JACYVU010000254.1  47934147-47934315
            858-922             JACYVU010000254.1  47936626-47936690
            923-1060            JACYVU010000254.1  47945870-47946007
            1061-4342           JACYVU010000254.1  47948844-47952125
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q22"
     gene            1..4342
                     /gene="Spred2"
                     /note="sprouty-related, EVH1 domain containing 2"
                     /db_xref="GeneID:305539"
                     /db_xref="RGD:1309304"
     exon            1..510
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
     CDS             485..1717
                     /gene="Spred2"
                     /note="spred-2"
                     /codon_start=1
                     /product="sprouty-related, EVH1 domain-containing protein
                     2"
                     /protein_id="NP_001040559.1"
                     /db_xref="GeneID:305539"
                     /db_xref="RGD:1309304"
                     /translation="MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVC
                     KVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTF
                     QSPADARAFDRGVRKAIEDLIEGSTTSSSTIHNEAELGDDDVFTTATDSSSNSSQKRE
                     PNTRTISSPTSCEHRRIYTLDPYPMDLYHPDQRLPRSYPQVTFPEDDEEIVRINPREK
                     IWMTGYEDYRHAPVRGKYLDSTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPK
                     GNVIKTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSC
                     MWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRAC
                     HHCGVMCRCCGGKHKAAA"
     exon            511..688
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
     exon            689..857
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
     exon            858..922
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1060
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..4342
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aatatggagt aaatcggtgg agtggcgaga tggggctcgg acggggcgcc ctgcaccgcc
       61 tcccaggcgc acctcccccg gctgccgagt gctacccgga accctgattg gcccggtccc
      121 cgggggcgac cccggcccgg ccccagcaca gccggctgcc cgccgccccg agcgtgcttg
      181 tttttctgct gctcttcccc tcccggtccc cttcccaaat ctcgcccctg cacccgctcg
      241 gagccccggg gccgcgcccg gcgggcccac ggcgtccgct gccgggtgcg ccccttccca
      301 gacctcgccc tgcgctcggg gctctccatt tccccgcctg cctcggcgcc cccttcatct
      361 ctagctcccc cctccaccac caaatcagga gatgtcggag atgtgaagaa ggggggcgag
      421 cgcacaggaa gatgaaggga gccaggctgc acgccgcggg acaggcgtct aggtgaacaa
      481 gaaaatgacc gaagaaacac acccggacga tgacagctat attgtgcgtg tcaaggctgt
      541 ggttatgacc agagatgact ccagcggggg atggttccca caggaaggag gcgggatcag
      601 tcgcgtcggg gtgtgtaagg tcatgcaccc tgaaggcaac ggacggagcg gctttctcat
      661 ccatggcgaa cgacagaaag acaaactggt tgtattggaa tgctatgtca gaaaggactt
      721 ggtctacacc aaagccaatc cgacgtttca ccattggaag gttgataata ggaagtttgg
      781 acttactttc caaagccctg cggatgcacg agcctttgac aggggcgtga gaaaagccat
      841 cgaagacctt atagaaggtt caacaacctc ctcttccacg atccataacg aagctgagct
      901 cggagacgat gacgttttta caactgccac agacagttct tctaattcct cgcagaagag
      961 ggagccgaat acaaggacaa tctcctcccc cacatcgtgc gagcaccgga ggatttatac
     1021 ccttgacccg taccccatgg atctttacca ccccgaccag cgtttgccgc ggtcgtaccc
     1081 ccaggtcacc ttcccggaag atgatgaaga gatagtacgc atcaaccccc gagagaagat
     1141 ctggatgacg ggttacgaag attaccggca cgcgccagtc cgcggcaaat acttggacag
     1201 caccgaagac gcagactcct acgtgcgctt cgccaagggt gaagtcccca aacacgaata
     1261 tacgtatccc tacgttgatt cttcggactt tggcttcggc gaggatccca aaggcaatgt
     1321 gatcaagaca cagccgccca gggccaagtc ccgtcggcgg aaggagaacg gcgaacggtc
     1381 gcggtgcgtg tactgcaggg acatgttcaa ccacgaagag aacagaaggg gccactgcca
     1441 agacgcgccc gacgccgtga gaacgtgcat tcgccgggtg agctgtatgt ggtgcgcgga
     1501 cagcatgctg taccactgca tgtccgaccc cgagggagac tacactgacc cttgttcgtg
     1561 cgacacaagc gatgagaagt tttgcctccg gtggatggca ctaattgcct tgtctttctt
     1621 ggctccttgt atgtgctgtt acctgcccct ccgggcctgc caccactgtg gagtgatgtg
     1681 caggtgctgt ggtgggaagc acaaagccgc tgcgtgactc ggtttccttc ctctcccctt
     1741 ctccaaccac agccacaggc atctcgtctc ctgcgtacag ccccacccca cccacccacc
     1801 ccgcccccat cttctccctg gctcccttcc aactccaaaa ggcaaggcca atcagaggag
     1861 gccaggctct ggtcccttgg tgacatcatt ccaaacccgg ggtagctgcc accccccccc
     1921 accccccacc cccccgtgtt gcagtgttct gaggaaagga gccttcggca tagactcgtg
     1981 tatttttcca gcctataatc atgctgtctt tcctgagtgt tggtttccaa ccccctcctc
     2041 cctacctttc tggttctgct gtcttggagg ggtttattga tttcccccca cccacccacc
     2101 cctgccccgt gacctttttt ttttctcttt cttttctccc cattgggctt gcctgtcgct
     2161 aactatcata ggtccctcag agatgtgacc gaatgatgaa gccatcaccc aggatgttgt
     2221 tattcgagga gatgagctag ataacaagtc tgtaccataa aacaataata ctttaagtcc
     2281 taaagccaaa tctcccgtga tgcccggggg ggtggggggg tggggggccc ttaatccgcc
     2341 atcatctctt cccctcccct ccccccacac gaacccattc actgattcaa aacagtcacc
     2401 ttttaaatga cttttttgag ccttaactcc agattaactt gtgagaatca agaacaaatc
     2461 cctcaaattg ctttgcatcc tcttggacca gggcttgcaa ggggatttgg gtttctatga
     2521 gcctgcccag tttaccccgt aaaataggac ttttttagaa tcccatcgcc acttctaaaa
     2581 tcctagtaac acatcagcga gataactttt attcgttatt ttacaaacca aatcggttta
     2641 ctcaattaaa aaccagtgtc ctgtgaacaa gttccagttg gaaaacacta cagttgtgga
     2701 tagaaccgta gccattccca gccccctaca ctgttttaca ttattcaggg ggggggggca
     2761 tttttaaata ccatcgattc aaagagcggt ggcttgctct ttttttttag gtgaatgttt
     2821 ttgttttgga ggcttttgtt ttttagtttg aggctcaatt ctttatttat acttgaaaaa
     2881 caaacaaaca aggccatatg taaagaaaaa aaccctttca atgcctaagt gtcttttctt
     2941 ccaccactcc acacccacat ttgccacttg gggtgcacag ataagggtag gtcaccaact
     3001 gattatgcct gtcgccgagc catacaaaag gcttcttatt cggggggggg gtatatatat
     3061 atatataaag gaatagccaa ctcttactga aaaaagacaa gcaaaagtgg ttacctatac
     3121 ctagcatggt acaatctaaa tttcatgggg actgtagggg acaggcttgt caccactggc
     3181 cgttcttcct atagtgatta acggtggagg gccgactcag tcccctttaa tccctaaacc
     3241 gccctctccc ccttattttt ccagtctgtc aatcactctg ggaaaatgct agccatgcat
     3301 accttcctgg caatgttttc tgctcgcctg acagcctgcc gtgtttcttc agacggatca
     3361 tcggtgtaga tagaatcagc atgcttctgc gttcgttggg aggtgttttt ctttgttggg
     3421 atgactggtt ggggctgtcg taggaaaggt gcagggggct gccatctccg ccatgtggag
     3481 agaggggtct ccatgctagg agactctaga ctgcctccag ccagcctcca gtgctgtgga
     3541 gagactgtcc cagcctgagg gactctcctt gggccctctc acctctctac aaagcacaac
     3601 actaatctgt ttcctcagac ctctgttcaa gtgcccctat ttatttcgat aagaatgcgc
     3661 atccctgtct gtcgccctgt ggtgtcgggc gcatccctgt ccggcgcccc gcggtgtcgc
     3721 gcgccgctct gccgtatccg gcacccgcag tgtcccgcgc cttgcagtgt cgcctctgtc
     3781 ctcgtttgtg cttccctctt cccttgctgg ttcccaccct cctaagagtt atgctagtgg
     3841 atcttactcc gtgcatcatc gtcttttggt tcgtgaaggc agcggttaag ggcacaaaga
     3901 tagccatggg gacatttatg taaatacgtt taattgccac actgcagctg aacggtgtgt
     3961 agtattttcc cagtcagctt tgccatactg acgtcaatca tttgagagaa attattcaga
     4021 ttttattttt atatccgtgg taacaaaacc taaccaaaag gtttcctgtt gagaagtttc
     4081 cctgactctt atcccaagct attcgctcac attaacaaat taaagtgcct gaagcaaaat
     4141 tcatttacct gtacactaaa acccctgttg tattgatttt tttttataat aagccttttt
     4201 acctctgtaa aaaaatatat atatacaagt gtatgatgta cattttagtt cttaactttt
     4261 tttatggttt ctaatatgta tgaccaatgt agccattgct ttgaaatgta ccatgtaaat
     4321 ataaacacat cctatcagac ca
//
