LOCUS       NM_001401073            2855 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus signal peptide peptidase-like 2B (Sppl2b),
            transcript variant 1, mRNA.
ACCESSION   NM_001401073 XM_006240947
VERSION     NM_001401073.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Fluhrer,R., Martin,L., Klier,B., Haug-Kroper,M., Grammer,G.,
            Nuscher,B. and Haass,C.
  TITLE     The alpha-helical content of the transmembrane domain of the
            British dementia protein-2 (Bri2) determines its processing by
            signal peptide peptidase-like 2b (SPPL2b)
  JOURNAL   J Biol Chem 287 (7), 5156-5163 (2012)
   PUBMED   22194595
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Behnke,J., Schneppenheim,J., Koch-Nolte,F., Haag,F., Saftig,P. and
            Schroder,B.
  TITLE     Signal-peptide-peptidase-like 2a (SPPL2a) is targeted to
            lysosomes/late endosomes by a tyrosine motif in its C-terminal tail
  JOURNAL   FEBS Lett 585 (19), 2951-2957 (2011)
   PUBMED   21896273
REFERENCE   3  (bases 1 to 2855)
  AUTHORS   Martin,L., Fluhrer,R. and Haass,C.
  TITLE     Substrate requirements for SPPL2b-dependent regulated intramembrane
            proteolysis
  JOURNAL   J Biol Chem 284 (9), 5662-5670 (2009)
   PUBMED   19114711
REFERENCE   4  (bases 1 to 2855)
  AUTHORS   Martin,L., Fluhrer,R., Reiss,K., Kremmer,E., Saftig,P. and Haass,C.
  TITLE     Regulated intramembrane proteolysis of Bri2 (Itm2b) by ADAM10 and
            SPPL2a/SPPL2b
  JOURNAL   J Biol Chem 283 (3), 1644-1652 (2008)
   PUBMED   17965014
REFERENCE   5  (bases 1 to 2855)
  AUTHORS   Nyborg,A.C., Ladd,T.B., Jansen,K., Kukar,T. and Golde,T.E.
  TITLE     Intramembrane proteolytic cleavage by human signal peptide
            peptidase like 3 and malaria signal peptide peptidase
  JOURNAL   FASEB J 20 (10), 1671-1679 (2006)
   PUBMED   16873890
REFERENCE   6  (bases 1 to 2855)
  AUTHORS   Friedmann,E., Hauben,E., Maylandt,K., Schleeger,S., Vreugde,S.,
            Lichtenthaler,S.F., Kuhn,P.H., Stauffer,D., Rovelli,G. and
            Martoglio,B.
  TITLE     SPPL2a and SPPL2b promote intramembrane proteolysis of TNFalpha in
            activated dendritic cells to trigger IL-12 production
  JOURNAL   Nat Cell Biol 8 (8), 843-848 (2006)
   PUBMED   16829952
REFERENCE   7  (bases 1 to 2855)
  AUTHORS   Fluhrer,R., Grammer,G., Israel,L., Condron,M.M., Haffner,C.,
            Friedmann,E., Bohland,C., Imhof,A., Martoglio,B., Teplow,D.B. and
            Haass,C.
  TITLE     A gamma-secretase-like intramembrane cleavage of TNFalpha by the
            GxGD aspartyl protease SPPL2b
  JOURNAL   Nat Cell Biol 8 (8), 894-896 (2006)
   PUBMED   16829951
REFERENCE   8  (bases 1 to 2855)
  AUTHORS   Krawitz,P., Haffner,C., Fluhrer,R., Steiner,H., Schmid,B. and
            Haass,C.
  TITLE     Differential localization and identification of a critical
            aspartate suggest non-redundant proteolytic functions of the
            presenilin homologues SPPL2b and SPPL3
  JOURNAL   J Biol Chem 280 (47), 39515-39523 (2005)
   PUBMED   15998642
REFERENCE   9  (bases 1 to 2855)
  AUTHORS   Friedmann,E., Lemberg,M.K., Weihofen,A., Dev,K.K., Dengler,U.,
            Rovelli,G. and Martoglio,B.
  TITLE     Consensus analysis of signal peptide peptidase and homologous human
            aspartic proteases reveals opposite topology of catalytic domains
            compared with presenilins
  JOURNAL   J Biol Chem 279 (49), 50790-50798 (2004)
   PUBMED   15385547
REFERENCE   10 (bases 1 to 2855)
  AUTHORS   Petroff,O.A., Novotny,E.J., Ogino,T., Avison,M. and Prichard,J.W.
  TITLE     In vivo measurements of ethanol concentration in rabbit brain by 1H
            magnetic resonance spectroscopy
  JOURNAL   J Neurochem 54 (4), 1188-1195 (1990)
   PUBMED   2313285
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Feb 1, 2022 this sequence version replaced XM_006240947.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643292.28288.1,
                                           SRR26360181.1249982.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-88                JAXUCZ010000007.1  9507191-9507278     c
            89-208              JAXUCZ010000007.1  9502030-9502149     c
            209-391             JAXUCZ010000007.1  9501071-9501253     c
            392-481             JAXUCZ010000007.1  9500683-9500772     c
            482-621             JAXUCZ010000007.1  9500373-9500512     c
            622-764             JAXUCZ010000007.1  9500149-9500291     c
            765-861             JAXUCZ010000007.1  9499906-9500002     c
            862-978             JAXUCZ010000007.1  9499077-9499193     c
            979-1060            JAXUCZ010000007.1  9498018-9498099     c
            1061-1135           JAXUCZ010000007.1  9497852-9497926     c
            1136-1198           JAXUCZ010000007.1  9497721-9497783     c
            1199-1298           JAXUCZ010000007.1  9497534-9497633     c
            1299-1376           JAXUCZ010000007.1  9497161-9497238     c
            1377-1537           JAXUCZ010000007.1  9496652-9496812     c
            1538-2855           JAXUCZ010000007.1  9494593-9495910     c
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..2855
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /note="signal peptide peptidase-like 2B"
                     /db_xref="GeneID:362828"
                     /db_xref="RGD:1308556"
     exon            1..88
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..1780
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /EC_number="3.4.23.-"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; protein SPPL2b; protein SPP-like 2B;
                     presenilin-like protein 1"
                     /codon_start=1
                     /product="signal peptide peptidase-like 2B isoform 1
                     precursor"
                     /protein_id="NP_001388002.1"
                     /db_xref="GeneID:362828"
                     /db_xref="RGD:1308556"
                     /translation="MAAARLAASLLLLAAQVACEFGVLRVVPQSGGTRGRDYCILYNP
                     QWAHLPHDLNKVSLLKLRDLSTTQLCSHLDVPVEDFTNQIALVARGNCTFYEKVRLAQ
                     GSGAHGLLIVSKERLVPPRGNKTQYEEISIPVALLSHRDLQDIFRRFGHEVMVALYAP
                     SEPVMDYNMVIIFIMAVGTVALGGYWAGSHDVKKRYMKHKRDDVPEKQEDEAVDVTPV
                     MICVFVVMCCFMLVLLYYFYDRLVYVIIGIFCLASSTGLYSCLAPCVRKLPFCTCRVP
                     DNNLPYFHKRPQARMLLLALFCVTVSVVWGVFRNEDQWAWVLQDTLGIAFCLYMLRTI
                     RLPTFKACTLLLLVLFVYDIFFVFITPYLTKSGNSIMVEVATGPSNSSTHEKLPMVLK
                     VPRLNTSPLSLCDRPFSLLGFGDILVPGLLVAYCHRFDIQVQSSRIYFVACTIAYGLG
                     LLVTFVALVLMRHGQPALLYLVPCTLLTSCTVALWRREMGAFWTGSGFAKDAPQTPWA
                     APQGPVPPKDVDASLSEQPRGEELAQSPLATEEAGATDPAKDPDSPVAGPLSPSNGDQ
                     VQPIPVVTPGTSA"
     exon            89..208
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            209..391
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            392..481
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            482..621
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            622..764
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            765..861
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            862..978
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1060
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1135
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1198
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1298
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1376
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1537
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..2855
                     /gene="Sppl2b"
                     /gene_synonym="RGD1308556"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggaaacctt ccgcctagaa ctgcggtggg accggcggac atggcggcgg cgcggctggc
       61 cgcatccctg ctgctactcg cggcccaggt ggcctgtgag ttcggtgtac tgcgagttgt
      121 gccccaaagc ggtgggaccc ggggcaggga ctactgcatc ctttacaacc cacagtgggc
      181 ccacttgcct catgacctca acaaagtgtc tcttctgaag cttcgagact tgagcacgac
      241 tcagctctgt tcccacctcg atgttcctgt agaagacttc accaaccaga tcgcactggt
      301 ggctcggggc aactgtacct tctatgagaa agtgcggctg gcccagggca gtggggcaca
      361 tgggctgctg atagtcagca aggagagact ggtccctcca agaggcaaca agacacagta
      421 tgaggagata agcatccctg tggccctgct aagccacagg gaccttcaag acatcttcag
      481 gcgctttggc cacgaggtaa tggtagccct gtatgctccg agcgagccag tgatggacta
      541 caacatggtc atcatcttca tcatggccgt gggcaccgtc gcccttggtg gctactgggc
      601 tggcagtcat gatgtgaaga aaaggtacat gaagcataag cgagacgacg tccctgagaa
      661 gcaggaggat gaggcggtgg acgtgacgcc ggtcatgatc tgcgtgtttg ttgtcatgtg
      721 ctgcttcatg cttgtgctcc tctactactt ctatgaccgc ttggtctacg tgatcattgg
      781 gatcttctgc ctggcctcct ctaccggcct ctacagctgc ctggcaccct gtgtgcgcaa
      841 gctgcccttc tgcacgtgca gggtccccga caacaacctg ccgtacttcc acaagcgtcc
      901 acaggcccgc atgctgctgc tggccctctt ctgtgtcact gtctccgtgg tgtggggtgt
      961 cttccgtaat gaggaccagt gggcatgggt cctgcaggac accctgggca ttgccttctg
     1021 cctgtacatg ctgaggacca ttcgcctgcc caccttcaag gcctgcacac tgctgctgct
     1081 ggtgctcttc gtctacgaca tcttcttcgt cttcatcacc ccctacctga ccaagagcgg
     1141 caacagcatc atggtggagg tggccactgg gccctcgaac tcatccaccc atgagaagct
     1201 gcccatggtg ctgaaggtgc ccaggctgaa cacctcgcct ctctccctgt gtgaccggcc
     1261 cttctccctt ctgggctttg gagacatctt ggtgccaggg ctgctggtgg cctactgcca
     1321 caggtttgat atccaggtgc agtcctccag gatttacttt gtggcctgca ccattgccta
     1381 tggcctgggc ctcctggtga ccttcgtggc tctcgtcctc atgcgacatg gccagcctgc
     1441 actcctctac ttggtcccct gcacactgct gaccagctgc accgtggctc tgtggcgccg
     1501 ggagatgggc gccttctgga cgggcagcgg ttttgcgaag gatgcacctc agaccccgtg
     1561 ggctgcaccc cagggacctg tgcctccgaa ggatgtggat gcctctctct cggagcagcc
     1621 tcggggtgaa gagctggccc agagccccct ggccactgag gaggcaggag ccacagaccc
     1681 tgccaaggat cctgacagcc ctgtggccgg tcccctcagc ccctcaaatg gggaccaagt
     1741 ccagcctata cctgtggtga ccccagggac ctcggcctag gaagaggcaa tgtgagagct
     1801 gggccctcgc agccttccat gccacacaga tgccggccac ctgggacctg caggggacag
     1861 ggacagctgc cacccaccag agtgacagac ctgcgctatg tccctccaac atggtgctca
     1921 gccctctgag gcctcaagtc cgtactccct ccagctgcac cccagcgacc agctctgctt
     1981 cgcaccgggc cgtgagcttc gcctgctgcc cactccatgc aaggccatgg ctgccgttgg
     2041 tgctttgctg atgctcgccc tgcgggtgac ccatgaccgc tcctccctcc ataggatggg
     2101 gctctctggg gccagagcaa caaccccatg gagcaggctc ctgtgctggg agcagccttg
     2161 aggggcctag ataggggcac cctgcccaag ggcctgggct tatgcaaggg gaccagaccc
     2221 cttcaggaga gtccgagccg agatggagtt gaggttgctg gtcactgctg caagccagca
     2281 aacagcgcca gctccaggtt ctgcagccga gtaggagttg gccaggtgta gatggccacg
     2341 tccccagcaa atcgatcagc ccaggtgtgt gagtcttcca tgcaggggcc actgccctct
     2401 gccccagcag tggcttctgg actggcgtct gtcctctacc tcaccaacca ggctctggac
     2461 tccaggacag acctgcctgc aggagccctc cacacatgct gcatgtcact gaggctgaag
     2521 ccaaggtccc cacaggctgt gaccgatcgg gagccatggc cacccacact tgcatggaaa
     2581 agggtgtctg ttacaggctg acaggcagtg acagctgggt ggtcccccta gagctctggg
     2641 gggtggtagg ggaggactcc ctaggcaccc agtctaacca gaactgaggg gcagaggagt
     2701 gcaggcccag ccacctgctt ctgccattgg actgagccat tttgtactgg gtgtccaagc
     2761 agtcctgctc ctctgagtgg tggaggtgct ctgcggggtg cccgagggcc ctggcttttg
     2821 gttgtcaggg gattaaatac cttttcatac tcaca
//
