LOCUS       NM_001108533            5323 bp    mRNA    linear   ROD 01-MAR-2024
DEFINITION  Rattus norvegicus SPARC/osteonectin, cwcv and kazal like domains
            proteoglycan 2 (Spock2), mRNA.
ACCESSION   NM_001108533 XM_001055082 XM_001055146 XM_006256419 XM_342134
VERSION     NM_001108533.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Tarantino,C., Paolella,G., Cozzuto,L., Minopoli,G., Pastore,L.,
            Parisi,S. and Russo,T.
  TITLE     miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic
            stem cells
  JOURNAL   FASEB J 24 (9), 3255-3263 (2010)
   PUBMED   20439489
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Manabe,R., Tsutsui,K., Yamada,T., Kimura,M., Nakano,I., Shimono,C.,
            Sanzen,N., Furutani,Y., Fukuda,T., Oguri,Y., Shimamoto,K.,
            Kiyozumi,D., Sato,Y., Sado,Y., Senoo,H., Yamashina,S., Fukuda,S.,
            Kawai,J., Sugiura,N., Kimata,K., Hayashizaki,Y. and Sekiguchi,K.
  TITLE     Transcriptome-based systematic identification of extracellular
            matrix proteins
  JOURNAL   Proc Natl Acad Sci U S A 105 (35), 12849-12854 (2008)
   PUBMED   18757743
REFERENCE   3  (bases 1 to 5323)
  AUTHORS   Vannahme,C., Schubel,S., Herud,M., Gosling,S., Hulsmann,H.,
            Paulsson,M., Hartmann,U. and Maurer,P.
  TITLE     Molecular cloning of testican-2: defining a novel calcium-binding
            proteoglycan family expressed in brain
  JOURNAL   J Neurochem 73 (1), 12-20 (1999)
   PUBMED   10386950
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000324.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001108533.4.
            
            On or before Sep 17, 2022 this sequence version replaced
            XM_006256419.4, NM_001108533.2.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132265,
                              SAMD00132267 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-447               JACYVU010000324.1  23761212-23761658
            448-456             JACYVU010000324.1  23774727-23774735
            457-502             JACYVU010000324.1  23774967-23775012
            503-617             JACYVU010000324.1  23775206-23775320
            618-732             JACYVU010000324.1  23776856-23776970
            733-847             JACYVU010000324.1  23779019-23779133
            848-964             JACYVU010000324.1  23779537-23779653
            965-1183            JACYVU010000324.1  23780224-23780442
            1184-1246           JACYVU010000324.1  23782632-23782694
            1247-1384           JACYVU010000324.1  23783960-23784097
            1385-5323           JACYVU010000324.1  23784212-23788150
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20q11"
     gene            1..5323
                     /gene="Spock2"
                     /note="SPARC/osteonectin, cwcv and kazal like domains
                     proteoglycan 2"
                     /db_xref="GeneID:361840"
                     /db_xref="RGD:1308568"
     exon            1..447
                     /gene="Spock2"
                     /inference="alignment:Splign:2.1.0"
     CDS             259..1530
                     /gene="Spock2"
                     /note="testican-2; sparc/osteonectin, cwcv and kazal-like
                     domains proteoglycan 2; sparc/osteonectin, cwcv and
                     kazal-like domains proteoglycan (testican) 2"
                     /codon_start=1
                     /product="testican-2 precursor"
                     /protein_id="NP_001102003.2"
                     /db_xref="GeneID:361840"
                     /db_xref="RGD:1308568"
                     /translation="MRAPGSGRLALPLLLLAVVALAEGDAKGLKEGETPGNFMEDEQW
                     LSSISQYSGKIKHWNRFRDEVEDDYIKSWEDNQQGDEALDTTKDPCQKVKCSRHKVCV
                     AQGYQRAMCISRKKLEHRIKQPSLKLHGGKDSICKPCHMAQLASVCGSDGHTYSSVCK
                     LEQQACLSNKHLAVRCEGPCPCPTEQSTASTTDSKSETCTGQDLADLGDRLRDWFQLL
                     RENSKQNGSASSSSSPAGLDKSLGASCKDSIGWMFSKLDTSGDLFLDQTELAAINLDK
                     YEVCIRPFFNSCDTYKDGRVSTAEWCFCFWREKPPCLAELERTQIQEAAKKKPGIFIP
                     SCDEDGYYRKMQCDQSSGDCWCVDQLGLELTGTRMHGNPDCDDIVGFSGDFGSGVGWE
                     DEEEKETEEAGEEAEEEEGEAGEADDGGYIW"
     exon            448..456
                     /gene="Spock2"
                     /inference="alignment:Splign:2.1.0"
     exon            457..502
                     /gene="Spock2"
                     /inference="alignment:Splign:2.1.0"
     exon            503..617
                     /gene="Spock2"
                     /inference="alignment:Splign:2.1.0"
     exon            618..732
                     /gene="Spock2"
                     /inference="alignment:Splign:2.1.0"
     exon            733..847
                     /gene="Spock2"
                     /inference="alignment:Splign:2.1.0"
     exon            848..964
                     /gene="Spock2"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1183
                     /gene="Spock2"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1246
                     /gene="Spock2"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1384
                     /gene="Spock2"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..5323
                     /gene="Spock2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agttacagac acagcccccg ctggcgcagc ctctctgatt ctctctccct ctccgcgtcc
       61 agcgctgggc tttttcagac aagtgcatct gctaaccagg tcacatttca gccacgaccc
      121 accctccgtc agtcactgga gtcagacagc aggaggaggg ctgaggagga ccgcagcgct
      181 tcgccagcag cgcggtggag ggaggacctc aaatactgct gaagtccaga cgccccccag
      241 gtccaagccc agaccacgat gcgcgctccg ggatccgggc ggctggctct gccgctgctc
      301 ctcctcgccg tagtcgcttt ggctgaaggc gacgccaagg ggctcaagga gggcgagacc
      361 cccggcaatt tcatggaaga cgagcaatgg ctgtcgtcca tctcgcagta cagcggcaag
      421 attaagcact ggaaccgctt ccgagacgag gtagaggatg attacatcaa gagttgggag
      481 gacaatcagc aaggagacga agctctggat accaccaagg acccctgcca gaaggtgaag
      541 tgcagtcgtc acaaggtgtg tgtcgctcaa ggctaccagc gcgccatgtg tatcagccgc
      601 aagaagctgg aacacaggat caagcagcct tctttgaaac tccacggagg caaagactcc
      661 atctgcaagc cctgtcacat ggcccagcta gcttccgtct gtggttccga tggccatacc
      721 tacagttcag tgtgcaagct ggagcagcag gcatgtctga gtaacaagca cctggcggtg
      781 aggtgtgagg gcccctgccc ctgtcccacg gagcagtcta ctgcgtctac caccgacagc
      841 aagtcagaga cctgcacagg gcaggacctg gccgacctgg gggataggct tcgtgactgg
      901 ttccagctcc ttcgtgagaa ctcgaagcag aatggctcag ccagcagctc ctctagccct
      961 gcagggttgg acaagagcct aggggccagt tgcaaggact ccatcggttg gatgttctct
     1021 aagctggaca ccagtggaga tctcttcctg gaccagacag aactggctgc catcaacctg
     1081 gacaaatacg aggtctgcat ccgccccttc ttcaactcct gcgacaccta caaagatggc
     1141 cgagtctcta ctgctgagtg gtgcttctgt ttctggaggg agaagccccc ctgcctggca
     1201 gagctggagc gcacacagat ccaggaggca gccaagaaga agccaggtat cttcattccg
     1261 agctgtgatg aagatggtta ctatcggaag atgcagtgtg accagagcag tggtgactgc
     1321 tggtgcgtgg accagctggg cctggagctg accggcacac gcatgcatgg gaaccctgac
     1381 tgtgatgaca tcgtgggttt ctccggggac tttggaagcg gtgtcggctg ggaggatgag
     1441 gaggaaaagg agacagaaga ggcaggcgag gaggctgagg aggaggaggg tgaggcgggt
     1501 gaggcagatg acggaggcta catctggtag atggccctcg aaggcctcgg caggccaggg
     1561 tgccgacggc cagaaagaca ggccagaaga gacctggaca gcagcaggca acttagcaaa
     1621 tggatccgga agtcagcgtg aaggaccctg gctccagtgg ggactggaga gtgtggcttg
     1681 tgtgactggt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgatgtgag
     1741 tcacctagag gtcccttcct tcctgtggtt atttctttcc cattgttatc gatgttcaaa
     1801 tgcacacttt cacgcacatg gacacaccaa cagaccaaaa tcaatgtgat gtgatgcccc
     1861 agatcctgtc cctcaggcca acacctcgac ccaggtcctg ccctgctcaa tcaaccttac
     1921 cccctcctgc ctttcttcct tcctctcctg aggccagaga gggagggcag gaagctggaa
     1981 gctgagcatc ctccccaaat cgggctgatt tgatgtgaaa gccccaccat ggtgtgtggt
     2041 ggtgaggcag gacctgttca cacaacaagg cttacccttc tgctgacatc cagagagctg
     2101 cttgtgggtg tgtccagccg tgacctctgg gctgtacgtg gctaaggata gctatccatg
     2161 tggcccaaca caaaacagga aacttactta aaaatagggt tttttggggc tggggattta
     2221 gctcagtggt agagcgctta cctaggaagc gcaaggccct gggttcggtc cccagctccg
     2281 aaaaaaagaa ccaaaaaaaa aaaaaaatag ggttttttgg ttgctgcttt tgggtttttg
     2341 ataattggat tggggagttc ttgaatgtgg gctttgtaga tgacaatact attacaacat
     2401 caaaaggttg gacacaccca ataggttatc tcttagccta gtcctgagta accagagata
     2461 gcccagcaga acaggcagag accatgaggc agaggaggag ggtaggtccc ttgataccag
     2521 gcctcagatt aacccagtca gcacctgttg cctcctgggg ctctaggctg ctttcttggc
     2581 aaatgggggc tgtcatcatg cagttcagtt ttgagggctc gcagccatga cctctgtttc
     2641 ctggcaagag tccctttgtc ctaaggagca gaggaacaga aagcagcaca cgtctgccca
     2701 cctcctccct gaccctgtag gaaacgatcc cctcttgtcc agagaaaggt atgtctctga
     2761 gatcctgtga gaaagcatca ggaagctgtg acttcctgtg cccagagata gtcctggagg
     2821 agaggccccg ataccttagt cctctcaacc atggcttccc tctgtctcaa gggtccccaa
     2881 gatggcccta ctagagccgt gccccagact ctatttaaag ccccaccaca tatatgaata
     2941 gagagcaatg tatcagaggg gaccacactg ggcagctagg ccctgaggcc atgcctcagt
     3001 gtgaggggct tttacccaga tgccctgtgg ggttttgtgt gtttattgat tcccactgca
     3061 ccccctctca aaggaaattc cttaaaatat ttgatgaaga atttcttacc tgtacttggg
     3121 ccactgatac cctttggtat gtgcgtgcgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     3181 gtgtgtgtgt gtgagagaga gagagagaga gagagagaga gagagagaga gagagaacac
     3241 cgagggccac cctgacggct agggagagtg aggacagccc cctgtgtggt ggaaggctgg
     3301 gcagctactg gggttcttgt gctagggttt ccaggacaaa gccccagtct ggctgtcccc
     3361 cagcatctca ggggcactag ctggttacat ccactgagtg tggactttag ggaagagcct
     3421 catccttccc atcccttccc gccaccctgg aatggccacc ttccttgtca tctcctgggg
     3481 caaatacaga aatgccacta agctccataa agtgcttctc attccacaac acagttctga
     3541 ctctccgggt gactgtccct gatcccatcc ccttcctcct ccctcccttc ctcaggcctc
     3601 tgcaaccatc ctctggtccc tggctcagtc acatcagccc tctgcattat agaagcagcc
     3661 atccctcctc ctcctcctcc tcctcctcct cctcctcttc ctcctcctcc tcctcctctt
     3721 cctcctcctc ctcctcctct tcctcctcct cttcctcctc ctcctcctcc tcttcctcct
     3781 cctcttcctc ctcctcctcc tcctcttcct cctcctcctc cccttccccc accccctcct
     3841 ccagctggag ttacttgaaa cttcctgctc tcgataggac ggagtctgat tcttcctctg
     3901 ccgtagctgg attcccagaa catcccgggt agctgtgaag gatttcagac tttctggggt
     3961 ggggatgggg tgttgtggag gaagaagcat ccccttcttt cgccctgccc cactgagggg
     4021 gctgtgctct gtgtgtccct ggatcttctg gttttgtatc tgactttgta aatatgtttt
     4081 aggaagaaag cagaaggtat ggagcttgac tgtggcaggc ctcaggggtc cagaagtttg
     4141 tccccctcta aagtgtcccc cccgtactgc ccttcgcccc cactgagact gagtctcaca
     4201 agggtagaca gagcagttgt ccctggtggg gccgaaggtc cccaagtagc ttctggtttc
     4261 ctcaggaacg aggtcccagg ccagactatt gtcagtccta aggcctcatc cttgaataag
     4321 ctgcctaccc agttccccca tgctcctgcc tgctctcgct cccttgctat gggcaggaga
     4381 gctagggctg tgctggaaag agttcagcca aactacagca gcaccccgcc ttgccgggcc
     4441 ctggagctgc cctactaaga ccctccatag cccttgggtg ctgggaggga tggcgtaagt
     4501 tggggcagtt tgaggttgga gggagtaaat gtgccttgag agacactcag aggtcccagg
     4561 gagctctggc aggaggcaaa gttggtgaga gagtcatggg acatcttgac ttccctccag
     4621 tgtttcatag gagtccacgt gatgggtggg cacaggtccg tccttactcc ggccccctcc
     4681 ctcccctggt ttgggttggt acccgtctgg cttttcttga gctttatctg ctttcggctt
     4741 ctcctctaag tgggtagatg gggtcttttg atatcggctc cccaaagcct gagcccacag
     4801 gcctgggaga gaaccagggg cccgtctttg atacctgggt catcacgaac ccttctgcct
     4861 gcagttgtcc caaggaagga cctgggggtg caaaaggaga ttcaagacat ctgtaacaag
     4921 tcaggcagag caacccgctt cttagcccgt gtcccactta aactgagctt agagttaaac
     4981 attgtggtca agtgcttggg ttccgtctgt agatttaagt gctgacgttg tatttcccag
     5041 taatcttaga tgtcttttga aaaactgaac aagtgttaga cctgtgtgtg cgttggtggg
     5101 cactcaagca tcccatgggt gaccccgttc tctccccttc ctccgtgaca catccactcc
     5161 tgccccgccc acccccactt gcctccaccc ggggcccctc actttgctgt ggtctttatc
     5221 tgcctgttac tcagcctaag gaaacaagtt cactctacac acgcataaaa gaaagcaaat
     5281 gttattttta agaaaatgga aaataaaaac tttataaaca tca
//
