LOCUS       NM_001415816            1713 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus SPO11 initiator of meiotic double strand breaks
            (Spo11), mRNA.
ACCESSION   NM_001415816 XM_006235698
VERSION     NM_001415816.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Finsterbusch,F., Ravindranathan,R., Dereli,I., Stanzione,M.,
            Trankner,D. and Toth,A.
  TITLE     Alignment of Homologous Chromosomes and Effective Repair of
            Programmed DNA Double-Strand Breaks during Mouse Meiosis Require
            the Minichromosome Maintenance Domain Containing 2 (MCMDC2) Protein
  JOURNAL   PLoS Genet 12 (10), e1006393 (2016)
   PUBMED   27760146
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Ishiguro,K., Kim,J., Shibuya,H., Hernandez-Hernandez,A., Suzuki,A.,
            Fukagawa,T., Shioi,G., Kiyonari,H., Li,X.C., Schimenti,J., Hoog,C.
            and Watanabe,Y.
  TITLE     Meiosis-specific cohesin mediates homolog recognition in mouse
            spermatocytes
  JOURNAL   Genes Dev 28 (6), 594-607 (2014)
   PUBMED   24589552
REFERENCE   3  (bases 1 to 1713)
  AUTHORS   Kogo,H., Tsutsumi,M., Ohye,T., Inagaki,H., Abe,T. and Kurahashi,H.
  TITLE     HORMAD1-dependent checkpoint/surveillance mechanism eliminates
            asynaptic oocytes
  JOURNAL   Genes Cells 17 (6), 439-454 (2012)
   PUBMED   22530760
REFERENCE   4  (bases 1 to 1713)
  AUTHORS   Kumar,R., Bourbon,H.M. and de Massy,B.
  TITLE     Functional conservation of Mei4 for meiotic DNA double-strand break
            formation from yeasts to mice
  JOURNAL   Genes Dev 24 (12), 1266-1280 (2010)
   PUBMED   20551173
REFERENCE   5  (bases 1 to 1713)
  AUTHORS   Zalzman,M., Falco,G., Sharova,L.V., Nishiyama,A., Thomas,M.,
            Lee,S.L., Stagg,C.A., Hoang,H.G., Yang,H.T., Indig,F.E.,
            Wersto,R.P. and Ko,M.S.
  TITLE     Zscan4 regulates telomere elongation and genomic stability in ES
            cells
  JOURNAL   Nature 464 (7290), 858-863 (2010)
   PUBMED   20336070
REFERENCE   6  (bases 1 to 1713)
  AUTHORS   Soper,S.F., van der Heijden,G.W., Hardiman,T.C., Goodheart,M.,
            Martin,S.L., de Boer,P. and Bortvin,A.
  TITLE     Mouse maelstrom, a component of nuage, is essential for
            spermatogenesis and transposon repression in meiosis
  JOURNAL   Dev Cell 15 (2), 285-297 (2008)
   PUBMED   18694567
REFERENCE   7  (bases 1 to 1713)
  AUTHORS   Li,X.C. and Schimenti,J.C.
  TITLE     Mouse pachytene checkpoint 2 (trip13) is required for completing
            meiotic recombination but not synapsis
  JOURNAL   PLoS Genet 3 (8), e130 (2007)
   PUBMED   17696610
  REMARK    Erratum:[PLoS Genet. 2007 Sep 7;3(9):e168. Li, Xin [corrected to
            Li, Xin Chenglin]]
REFERENCE   8  (bases 1 to 1713)
  AUTHORS   Liebe,B., Petukhova,G., Barchi,M., Bellani,M., Braselmann,H.,
            Nakano,T., Pandita,T.K., Jasin,M., Fornace,A., Meistrich,M.L.,
            Baarends,W.M., Schimenti,J., de Lange,T., Keeney,S.,
            Camerini-Otero,R.D. and Scherthan,H.
  TITLE     Mutations that affect meiosis in male mice influence the dynamics
            of the mid-preleptotene and bouquet stages
  JOURNAL   Exp Cell Res 312 (19), 3768-3781 (2006)
   PUBMED   17010969
REFERENCE   9  (bases 1 to 1713)
  AUTHORS   Guillon,H., Baudat,F., Grey,C., Liskay,R.M. and de Massy,B.
  TITLE     Crossover and noncrossover pathways in mouse meiosis
  JOURNAL   Mol Cell 20 (4), 563-573 (2005)
   PUBMED   16307920
REFERENCE   10 (bases 1 to 1713)
  AUTHORS   Di Giacomo,M., Barchi,M., Baudat,F., Edelmann,W., Keeney,S. and
            Jasin,M.
  TITLE     Distinct DNA-damage-dependent and -independent responses drive the
            loss of oocytes in recombination-defective mouse mutants
  JOURNAL   Proc Natl Acad Sci U S A 102 (3), 737-742 (2005)
   PUBMED   15640358
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Jan 3, 2023 this sequence version replaced XM_006235698.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643293.20718.1,
                                           SRR26360181.295739.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-189               JAXUCZ010000003.1  182175856-182176044
            190-303             JAXUCZ010000003.1  182178022-182178135
            304-392             JAXUCZ010000003.1  182179935-182180023
            393-459             JAXUCZ010000003.1  182180632-182180698
            460-568             JAXUCZ010000003.1  182181341-182181449
            569-655             JAXUCZ010000003.1  182181818-182181904
            656-692             JAXUCZ010000003.1  182182558-182182594
            693-802             JAXUCZ010000003.1  182182897-182183006
            803-902             JAXUCZ010000003.1  182184580-182184679
            903-940             JAXUCZ010000003.1  182185057-182185094
            941-1017            JAXUCZ010000003.1  182185662-182185738
            1018-1129           JAXUCZ010000003.1  182187764-182187875
            1130-1713           JAXUCZ010000003.1  182188687-182189270
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q42"
     gene            1..1713
                     /gene="Spo11"
                     /note="SPO11 initiator of meiotic double strand breaks"
                     /db_xref="GeneID:366261"
                     /db_xref="RGD:1306454"
     exon            1..189
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..1249
                     /gene="Spo11"
                     /EC_number="5.6.2.3"
                     /note="sporulation protein, meiosis-specific, SPO11
                     homolog; SPO11 meiotic protein covalently bound to DSB
                     homolog; SPO11, initiator of meiotic double stranded
                     breaks"
                     /codon_start=1
                     /product="meiotic recombination protein SPO11"
                     /protein_id="NP_001402745.1"
                     /db_xref="GeneID:366261"
                     /db_xref="RGD:1306454"
                     /translation="MALAPMGPEASFFDALDRHRASLLAMVKKGAGETPDRATRVPSS
                     SEVLTSIENIIQDIIKSLARNEAPAFTIDNRSSWENMTFEDAFGLQMRPQCTTRKIKS
                     DSLRSVRKFALILKILSMIYKLIQSNTYATKRDIYYTESQLFRNQAAVDSIIDDISCM
                     LKVPRRSLHVISTSKGLIAGNLRYVEEDGTQVQCMCSATATAVPSNIQGMQNLVTDAK
                     FLLIVEKDATFQRLLDDNFCSRMSPCIMVTGKGTPDLNTRLLVKKLWDTFHIPVFTLV
                     DADPYGIEIMCIYKYGSMSMSFEAHNLTVPTIRWLGLLPSDIKRLNIPKDSLIPLTRR
                     DQMKLDSILRRPYITYHPFWKKEMEMLADSKTKAEIQALSFLSSDYLSRVYLPNKLRF
                     GGWI"
     exon            190..303
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     exon            304..392
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     exon            393..459
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     exon            460..568
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     exon            569..655
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     exon            656..692
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     exon            693..802
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     exon            803..902
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     exon            903..940
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1017
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1129
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1713
                     /gene="Spo11"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agagcgcggg aaggttcgca ggggcaggag gcctccgagc agctcagcgt cagccgtcat
       61 ggcgttagcg cctatggggc ccgaggcctc gttcttcgac gccttggatc ggcacagggc
      121 ttccctgttg gccatggtga agaaaggcgc aggggagacc cctgacaggg ccacccgtgt
      181 gccctctagt tctgaggttc ttacgtctat agaaaatatt atccaagaca taatcaaaag
      241 cttggcaaga aatgaagcgc ccgccttcac aatagacaac agatcgagct gggaaaacat
      301 gacgttcgaa gatgcttttg gccttcagat gagacctcag tgtaccacaa gaaaaatcaa
      361 aagcgattca ctaagatcag ttagaaaatt tgctctaatt ctgaaaatat tgtccatgat
      421 ttataaatta atacagagca acacttacgc aaccaagaga gacatatact acactgagag
      481 ccagctcttc aggaaccagg ctgcagtgga cagcattata gatgacattt cctgtatgct
      541 gaaagtgccc aggaggagtc tgcatgtgat atctacatcg aagggattga ttgctggcaa
      601 cttgagatac gtggaggaag atggtaccca agtccagtgc atgtgtagtg ccacggctac
      661 tgctgtgcca tctaacattc aagggatgca gaatctggtc acagatgcga agtttctgct
      721 aatcgtagag aaggatgcaa catttcagcg gctcctggac gacaacttct gcagcaggat
      781 gtccccgtgc atcatggtta cgggaaaggg tactccagat ctgaacacga ggctcttggt
      841 aaagaagctg tgggacacct ttcatattcc tgtcttcaca ctggtagatg ctgaccccta
      901 cggcatcgag ataatgtgca tctataagta cggatccatg tccatgtctt ttgaagccca
      961 caatctcact gtcccaacaa tcagatggct tggtctcctc ccttctgata ttaaaaggtt
     1021 aaatatacct aaggatagtt tgatcccact gacaaggcgt gaccagatga agctggacag
     1081 tatcctgagg aggccttaca ttacctatca ccccttctgg aaaaaagaga tggagatgct
     1141 ggccgactct aagacgaagg cggagatcca agccttgagc ttcctgtcgt cagactatct
     1201 gtccagagtg tacttgccca acaagctgag gtttggaggg tggatatgaa accacagtgg
     1261 gggaccgtga gcctccagag ccctcattgg cctgtttgaa tgataaacac tgggctaagg
     1321 agtgtcgagt cttagttctg ccacagtaaa aatcatttta cattgacata tttttgtcaa
     1381 agcaaacttc atagtgctct ttagaaggtt tgatcttcag taaagacaga tttttattct
     1441 atgttctaga gtgattataa aataatttat ttaattttaa atgttttatt agaaaaactg
     1501 tcacatatcg tcaaagtcta taaaaatatt tacttcctac agcacagctt gtgcagcatg
     1561 aaatgtctca ctagtaacag aatgcaagcg gccctgagag ggggcaacga aagggtcaca
     1621 aggcgccgtt tgctagtact tcctcttgcc tcatgtacag gcaccttgcc ctgttaatgc
     1681 atccttgaaa ctaataaaac ttattataaa taa
//
