LOCUS       NM_001012066            2408 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus sphingosine kinase 2 (Sphk2), mRNA.
ACCESSION   NM_001012066 XM_218558
VERSION     NM_001012066.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Dominguez,G., Maddelein,M.L., Pucelle,M., Nicaise,Y., Maurage,C.A.,
            Duyckaerts,C., Cuvillier,O. and Delisle,M.B.
  TITLE     Neuronal sphingosine kinase 2 subcellular localization is altered
            in Alzheimer's disease brain
  JOURNAL   Acta Neuropathol Commun 6 (1), 25 (2018)
   PUBMED   29615132
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Zhang,R., Li,L., Yuan,L. and Zhao,M.
  TITLE     Hypoxic preconditioning protects cardiomyocytes against
            hypoxia/reoxygenation-induced cell apoptosis via sphingosine kinase
            2 and FAK/AKT pathway
  JOURNAL   Exp Mol Pathol 100 (1), 51-58 (2016)
   PUBMED   26621495
  REMARK    GeneRIF: hypoxic preconditioning significantly increased SphK2
            expression in primary cardiomyocytes
REFERENCE   3  (bases 1 to 2408)
  AUTHORS   Sivasubramanian,M., Kanagaraj,N., Dheen,S.T. and Tay,S.S.
  TITLE     Sphingosine kinase 2 and sphingosine-1-phosphate promotes
            mitochondrial function in dopaminergic neurons of mouse model of
            Parkinson's disease and in MPP+ -treated MN9D cells in vitro
  JOURNAL   Neuroscience 290, 636-648 (2015)
   PUBMED   25637806
REFERENCE   4  (bases 1 to 2408)
  AUTHORS   Gandy,K.A., Canals,D., Adada,M., Wada,M., Roddy,P., Snider,A.J.,
            Hannun,Y.A. and Obeid,L.M.
  TITLE     Sphingosine 1-phosphate induces filopodia formation through S1PR2
            activation of ERM proteins
  JOURNAL   Biochem J 449 (3), 661-672 (2013)
   PUBMED   23106337
REFERENCE   5  (bases 1 to 2408)
  AUTHORS   Hisano,Y., Kobayashi,N., Kawahara,A., Yamaguchi,A. and Nishi,T.
  TITLE     The sphingosine 1-phosphate transporter, SPNS2, functions as a
            transporter of the phosphorylated form of the immunomodulating
            agent FTY720
  JOURNAL   J Biol Chem 286 (3), 1758-1766 (2011)
   PUBMED   21084291
REFERENCE   6  (bases 1 to 2408)
  AUTHORS   Mizugishi,K., Yamashita,T., Olivera,A., Miller,G.F., Spiegel,S. and
            Proia,R.L.
  TITLE     Essential role for sphingosine kinases in neural and vascular
            development
  JOURNAL   Mol Cell Biol 25 (24), 11113-11121 (2005)
   PUBMED   16314531
REFERENCE   7  (bases 1 to 2408)
  AUTHORS   Maceyka,M., Sankala,H., Hait,N.C., Le Stunff,H., Liu,H., Toman,R.,
            Collier,C., Zhang,M., Satin,L.S., Merrill,A.H. Jr., Milstien,S. and
            Spiegel,S.
  TITLE     SphK1 and SphK2, sphingosine kinase isoenzymes with opposing
            functions in sphingolipid metabolism
  JOURNAL   J Biol Chem 280 (44), 37118-37129 (2005)
   PUBMED   16118219
REFERENCE   8  (bases 1 to 2408)
  AUTHORS   Kharel,Y., Lee,S., Snyder,A.H., Sheasley-O'neill,S.L., Morris,M.A.,
            Setiady,Y., Zhu,R., Zigler,M.A., Burcin,T.L., Ley,K., Tung,K.S.,
            Engelhard,V.H., Macdonald,T.L., Pearson-White,S. and Lynch,K.R.
  TITLE     Sphingosine kinase 2 is required for modulation of lymphocyte
            traffic by FTY720
  JOURNAL   J Biol Chem 280 (44), 36865-36872 (2005)
   PUBMED   16093248
REFERENCE   9  (bases 1 to 2408)
  AUTHORS   Okada,T., Ding,G., Sonoda,H., Kajimoto,T., Haga,Y.,
            Khosrowbeygi,A., Gao,S., Miwa,N., Jahangeer,S. and Nakamura,S.
  TITLE     Involvement of N-terminal-extended form of sphingosine kinase 2 in
            serum-dependent regulation of cell proliferation and apoptosis
  JOURNAL   J Biol Chem 280 (43), 36318-36325 (2005)
   PUBMED   16103110
REFERENCE   10 (bases 1 to 2408)
  AUTHORS   Liu,H., Sugiura,M., Nava,V.E., Edsall,L.C., Kono,K., Poulton,S.,
            Milstien,S., Kohama,T. and Spiegel,S.
  TITLE     Molecular cloning and functional characterization of a novel
            mammalian sphingosine kinase type 2 isoform
  JOURNAL   J Biol Chem 275 (26), 19513-19520 (2000)
   PUBMED   10751414
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC079120.1.
            
            On Feb 11, 2005 this sequence version replaced XM_218558.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079120.1, SRR8487227.212730.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307 [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q22"
     gene            1..2408
                     /gene="Sphk2"
                     /note="sphingosine kinase 2"
                     /db_xref="GeneID:308589"
                     /db_xref="RGD:1307757"
     exon            1..30
                     /gene="Sphk2"
                     /inference="alignment:Splign:2.1.0"
     exon            31..502
                     /gene="Sphk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..1947
                     /gene="Sphk2"
                     /EC_number="2.7.1.91"
                     /codon_start=1
                     /product="sphingosine kinase 2"
                     /protein_id="NP_001012066.1"
                     /db_xref="GeneID:308589"
                     /db_xref="RGD:1307757"
                     /translation="MAPPPLLPVAASTPILHGEFGSYPAHGPRFALTLTTQALHIQRL
                     RPKPEARPRDGLVPLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRA
                     DGAATYVENRAEAQRWATALMCLLRGVPLSGDQEITPELLPRKPRLLLLVNPFGGRGL
                     AWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEV
                     LNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQTVGVDLLLNCSLLLCRG
                     GSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLATL
                     HTYRGRLSYLPATTEPALPIPGHGLPRAKSELALAPAPAPAATHSPLHRSVSDLPLPL
                     PQPALASPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNAFKTAQLSP
                     IAEGPSEMPASGLLPPIHNAPEASTWGPADHLLPPLGSPLPQDWVTIEGEFVLMLAIL
                     PSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGYA
                     AARAFRLEPLTPRGVLTVDGELVEYGPIQAQVHPSLATLLTGPAGQKPRA"
     exon            503..652
                     /gene="Sphk2"
                     /inference="alignment:Splign:2.1.0"
     exon            653..747
                     /gene="Sphk2"
                     /inference="alignment:Splign:2.1.0"
     exon            748..863
                     /gene="Sphk2"
                     /inference="alignment:Splign:2.1.0"
     exon            864..2383
                     /gene="Sphk2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttcctggaga gaacttggcc agacacccag aggctggacc aggagctgac cacaagccaa
       61 gagtctagaa gtgcttggga ctgaaccaag gtcgtcatgg ccccaccacc actactgcca
      121 gtggctgcca gcactccgat cctgcacggc gagtttggtt cctacccagc ccatggcccc
      181 aggtttgccc tcaccctcac gacacaagcc ctacacatac agcgactgcg cccaaagcca
      241 gaagcccggc cccgagatgg tctagtccct ctggacgagg tctcaggctg tggcactctg
      301 cagagccgta gccccgaaga cactgcagcc tacttctgca tctacaccta cccacgtggc
      361 cggcgtgggg gccggcgcag agctactcgt accttccggg ccgacggggc cgccacttat
      421 gtggagaatc gtgcagaggc ccagcgttgg gcaactgctc tcatgtgtct cctccgagga
      481 gtgcctctgt ctggggacca ggaaatcacc cctgaattgc tgccccggaa gcccaggctg
      541 ctcctattgg tcaatccttt tggggggcgg ggcctggcct ggcagcgctg tatggaccac
      601 gtggtgccca tgatctctga agctgggctg tccttcaacc tcatacagac agaacgacaa
      661 aaccatgcac gtgagctggt gcaggggttg agcctgagtg agtgggaagg cattgtcact
      721 gtgtccggag atgggctgct ttatgaggtg ctgaatgggc tcctcgaccg cccagattgg
      781 gaagacgctg tgcggatgcc cattggtgtc ctcccctgtg gttcgggcaa tgcactagct
      841 ggggcagtta accatcatgg cgggtttgag caaactgttg gtgttgacct gttgctcaac
      901 tgctcgcttc ttctctgccg gggtggcagc catcctctgg acctgctctc tgtgacgcta
      961 gcctcaggat cccgctgttt ttccttcctg tcagttgcct ggggattctt gtcagatgtg
     1021 gacattcaca gcgagcgctt cagagccctg ggcagtgctc gattcacact gggtgcagtg
     1081 ctaggcctgg ccacattgca tacctaccgt ggacgtctct cctacctccc tgctaccaca
     1141 gaaccagcct tgcccatccc aggccatggt ctgccaagag ccaagtcaga actagccttg
     1201 gccccagccc cagccccagc cgccacccac tcacctcttc atcgatctgt gtctgacctg
     1261 cccctgcctc ttccccagcc tgccctggcc tcccctggct cccctgagcc cctgcctgac
     1321 ctgtccctca atggtggtgg tccagagctg actggagact gggggggagc tggggatgca
     1381 cctttgtccc cagacccatt gctgccttca tcccccaacg ctttcaaaac agctcagctt
     1441 tcacccatcg ccgaagggcc ctcagaaatg ccagcatcag ggctgctgcc tcccatccac
     1501 aatgctccag aagcctctac ctggggccca gcggaccacc tgctccctcc cctgggctct
     1561 ccactgcccc aagactgggt gacaatagag ggggagtttg tactcatgtt ggccatcttg
     1621 ccaagccacc tctgcgcaga cctgatggca gccccacacg cgcgttttga cgatggtgtg
     1681 gtgcacctgt gctgggtgcg gagcggcatc tcacgggccg cacttctacg aattttgctg
     1741 gccatggaac atggaaacca cttcagcctg ggctgcccac atctgggcta tgctgcagca
     1801 cgtgccttcc gccttgagcc actcacgcct cgtggggtgc tcactgtaga tggggaatta
     1861 gtagagtatg ggccaataca ggcacaggtg catccaagtc tcgccacact gctcactggg
     1921 cctgcaggtc aaaagcctcg agcctgaatg agcccaaagc atgccaagtt gatgggacca
     1981 gagccccata ggctaaggtc taccttttat aggtagaagt ggggcctgaa ctcagaactg
     2041 caagggtaga gagtgctcca gaccctcagt tcccagagga ccctagaggc tcgagggtgg
     2101 gatctgcctt tcttgatgtc caatgatggg gcctggaatg tatgagctag caaggcttct
     2161 tcagcttact gtccagtcag ggtttcttct tgcctatttc ggtgcctcta cttgactggc
     2221 caatcagccc ttgaggggca ggttccccca ggtggtccct agatttgcac taatgttccc
     2281 ctggccagtt aggggtggga tgttctgtgt cttgtgtgtc cctgtcccta gtctaaaaag
     2341 caattgaaaa ggtctatgca ataaaggtgt tgctttctct ggaaaaaaaa aaaaaaaaaa
     2401 aaaaaaaa
//
