LOCUS       NM_001399110            3687 bp    mRNA    linear   ROD 16-JUN-2024
DEFINITION  Rattus norvegicus SPG7 matrix AAA peptidase subunit, paraplegin
            (Spg7), transcript variant 2, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001399110 XM_008772619
VERSION     NM_001399110.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Shanmughapriya,S., Rajan,S., Hoffman,N.E., Higgins,A.M., Tomar,D.,
            Nemani,N., Hines,K.J., Smith,D.J., Eguchi,A., Vallem,S., Shaikh,F.,
            Cheung,M., Leonard,N.J., Stolakis,R.S., Wolfers,M.P., Ibetti,J.,
            Chuprun,J.K., Jog,N.R., Houser,S.R., Koch,W.J., Elrod,J.W. and
            Madesh,M.
  TITLE     SPG7 Is an Essential and Conserved Component of the Mitochondrial
            Permeability Transition Pore
  JOURNAL   Mol Cell 60 (1), 47-62 (2015)
   PUBMED   26387735
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Koppen,M., Bonn,F., Ehses,S. and Langer,T.
  TITLE     Autocatalytic processing of m-AAA protease subunits in mitochondria
  JOURNAL   Mol Biol Cell 20 (19), 4216-4224 (2009)
   PUBMED   19656850
REFERENCE   3  (bases 1 to 3687)
  AUTHORS   Ferreirinha,F., Quattrini,A., Pirozzi,M., Valsecchi,V., Dina,G.,
            Broccoli,V., Auricchio,A., Piemonte,F., Tozzi,G., Gaeta,L.,
            Casari,G., Ballabio,A. and Rugarli,E.I.
  TITLE     Axonal degeneration in paraplegin-deficient mice is associated with
            abnormal mitochondria and impairment of axonal transport
  JOURNAL   J Clin Invest 113 (2), 231-242 (2004)
   PUBMED   14722615
REFERENCE   4  (bases 1 to 3687)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   5  (bases 1 to 3687)
  AUTHORS   Kremmidiotis,G., Gardner,A.E., Settasatian,C., Savoia,A.,
            Sutherland,G.R. and Callen,D.F.
  TITLE     Molecular and functional analyses of the human and mouse genes
            encoding AFG3L1, a mitochondrial metalloprotease homologous to the
            human spastic paraplegia protein
  JOURNAL   Genomics 76 (1-3), 58-65 (2001)
   PUBMED   11549317
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On Jan 4, 2022 this sequence version replaced XM_008772619.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ224821.1, SRR26643289.42960.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-202               JAXUCZ010000019.1  68025619-68025820
            203-305             JAXUCZ010000019.1  68027781-68027883
            306-395             JAXUCZ010000019.1  68031713-68031802
            396-637             JAXUCZ010000019.1  68035806-68036047
            638-777             JAXUCZ010000019.1  68037461-68037600
            778-880             JAXUCZ010000019.1  68038652-68038754
            881-1006            JAXUCZ010000019.1  68040280-68040405
            1007-1169           JAXUCZ010000019.1  68040812-68040974
            1170-1343           JAXUCZ010000019.1  68041444-68041617
            1344-1468           JAXUCZ010000019.1  68048033-68048157
            1469-1571           JAXUCZ010000019.1  68049802-68049904
            1572-1682           JAXUCZ010000019.1  68050652-68050762
            1683-1798           JAXUCZ010000019.1  68052891-68053006
            1799-1955           JAXUCZ010000019.1  68054756-68054912
            1956-2122           JAXUCZ010000019.1  68056332-68056498
            2123-2200           JAXUCZ010000019.1  68057045-68057122
            2201-3687           JAXUCZ010000019.1  68058266-68059752
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..3687
                     /gene="Spg7"
                     /note="SPG7 matrix AAA peptidase subunit, paraplegin"
                     /db_xref="GeneID:353231"
                     /db_xref="RGD:727940"
     exon            1..202
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..2365
                     /gene="Spg7"
                     /note="isoform 2 is encoded by transcript variant 2;
                     paraplegin; spastic paraplegia 7 homolog; SPG7, paraplegin
                     matrix AAA peptidase subunit; mitochondrial inner membrane
                     m-AAA protease component paraplegin"
                     /codon_start=1
                     /product="mitochondrial inner membrane m-AAA protease
                     component paraplegin isoform 2"
                     /protein_id="NP_001386039.1"
                     /db_xref="GeneID:353231"
                     /db_xref="RGD:727940"
                     /translation="MAAALLLLRALRQSPEPGPWRLWAQLSGRSPGLFSGAGGRRPYV
                     VRGTPIGLAAAGGHTPQSLLLRILTPSFEGVSGLLLKRHIVPSAIRLWQLSGSTLYFN
                     TSGLKQKNKDDDKPKGKAPEDDEEERRRKEREDQMYRERLRTLFIIAIVMSLLNSLST
                     SGGSISWADFVNEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVAN
                     IDKFEEKLRAAEDELNIESKDRIPVSYKRTGFFGNALYALGMTAVGLAILWYVFRLAG
                     MTGREGGFSAFNQLKMARFTIVDGKTGKGVSFQDVAGMHEAKLEVREFVDYLKSPERF
                     LQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRS
                     LFKEARARAPCIVYIDEIDAVGKKRSTSMSGFSNTEEEQTLNQLLVEMDGMGTADHVI
                     VLASTNRADVLDNALMRPGRLDRHVFIDLPTLQERREIFEQHLKGLKLTQPSSFYSQR
                     LAELTPGFSGADIANICNEAALHAAREGHTSVHTFNFEYAVERVIAGTAKKSKILSKE
                     EQRVVAFHESGHALVGWLLEHTEAVMKVSIAPRTNAALGFSQMLPRDQYLFTKEQLFE
                     RMCMALGGRAAEAISFSRVTSGAQDDLRKVTRIAYSMVKQFGMAPSIGPVSFPEAQEG
                     LVGIGRRPFSQGLQQMMDHEARLLVARAYRHTEKVLLDNLDKLQALANALLEKEVINY
                     EDIEALIGPPPHGPKKMIAPQKWIDAEKEKQASGEEEAPAP"
     exon            203..305
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            306..395
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            396..637
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            638..777
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            778..880
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1006
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1169
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1343
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1468
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1571
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1682
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1798
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1955
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2122
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2200
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..3687
                     /gene="Spg7"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggcgccgct ctcggcacca tggcggccgc gttgctgctg cttcgcgcgc tccgccagag
       61 cccggagccc gggccctggc ggctgtgggc ccagctctca ggcaggagcc ccggcctctt
      121 ctccggggcc ggcggtaggc ggccgtacgt agtccgtggg actccgattg gcctggctgc
      181 ggctggaggc cacacgcctc agagcttgct attgcgaata ctgaccccta gctttgaagg
      241 tgttagcgga ttgttactga aacggcatat agttccaagt gcaatcagac tgtggcagct
      301 ttcaggtagt actttgtatt ttaacacctc agggttgaag cagaagaaca aagatgatga
      361 taaacccaaa ggcaaagccc cggaagatga cgaagaagag aggaggcgga aggagcggga
      421 agaccagatg taccgggaga ggctgcgcac cttgttcatc atcgccatcg tgatgagcct
      481 gctcaactcc ctcagcacaa gtggcggcag catctcctgg gctgacttcg tcaacgagat
      541 gctggctaaa ggcgaggtgc agcgtgtgca ggtggtgccc gagagtgatg tggtggaagt
      601 ctacctgcat ccaggagctg tggtgtttgg gcggcctcgg ctggccttga tgtaccggat
      661 gcaggttgca aacatcgaca aatttgaaga gaagcttcga gctgctgaag atgaactgaa
      721 catcgagagc aaggacagga ttcccgtgtc ctacaagcgg acaggattct ttggaaatgc
      781 cctctatgcc ctggggatga ctgcagtggg cttggccatc ctgtggtatg ttttcagact
      841 ggctgggatg accggaaggg aaggcggatt cagtgccttt aatcagctta aaatggcgcg
      901 cttcaccatc gtggacggga agacggggaa aggagtcagc ttccaggatg tggcgggaat
      961 gcacgaagcc aagctggaag tccgagagtt tgtggattat ctgaagagcc cggagcgttt
     1021 ccttcagctg ggtgccaagg ttccaaaggg tgccctgctg ctgggacccc ctggttgtgg
     1081 gaagacgctg ttggccaagg cagtggccac ggaggctcag gtgccctttt tagcaatggc
     1141 tggcccagag tttgtggagg tcattggagg cctgggagcc gcccgagtgc gcagcctctt
     1201 caaggaagcc cgggccaggg ccccttgcat tgtgtacatt gatgagatcg atgctgtggg
     1261 gaagaagcgc tccacctcca tgtccgggtt ctccaacaca gaagaggagc agaccctcaa
     1321 ccagctcctc gtagagatgg acggaatggg caccgcagac cacgtcatcg tcttggcatc
     1381 caccaaccga gccgatgtcc tggacaacgc tctgatgagg cccggtcggc tcgacaggca
     1441 cgtcttcatc gatcttccca ctctccagga gaggcgggag attttcgagc agcacctgaa
     1501 aggcctcaag ttgacccagc ccagcagttt ttactcccag cggctggcag agctgactcc
     1561 tggattcagt ggcgccgaca tcgccaacat ctgcaacgag gctgcactgc acgctgcacg
     1621 cgaggggcac acatccgtcc acacgttcaa cttcgagtat gctgtggagc gcgtcattgc
     1681 tggaactgct aaaaagagta agatcctttc caaggaggag cagagagtgg ttgctttcca
     1741 tgagtctggc catgccctgg tcggttggct gctggaacac acggaggctg tgatgaaggt
     1801 ctccatagca cctcggacaa acgctgctct gggcttctct cagatgctcc ctcgggacca
     1861 gtacctcttc accaaggagc agctgtttga gcggatgtgc atggccctag gcggccgtgc
     1921 agctgaagcc atctcattca gcagggtcac ctctggggcc caggatgatt tgaggaaggt
     1981 tacccgtatc gcctactcca tggtgaaaca gtttgggatg gctcctagca ttgggcccgt
     2041 atccttccct gaggcacaag agggcctcgt gggcattgga cgccgtccct tcagccaggg
     2101 tctccagcag atgatggacc atgaagcaag actgctggtg gccagagcct acagacacac
     2161 tgagaaggtg ctgctggaca acctggacaa gctgcaggcg ctggcgaatg cccttctgga
     2221 aaaggaagtg ataaactatg aggacattga ggcgctcatt ggcccgccgc cccatgggcc
     2281 aaagaaaatg attgcaccac agaaatggat tgatgctgaa aaggagaaac aagcctcagg
     2341 ggaggaggag gctccggctc cctagccccc acctccaagg cccagataat ggcttcctcc
     2401 atgcacctgc agttatcaca gaacttgtca tagctgccct ccacctccga gatgcttttc
     2461 acctgaactt aaacctcagc ctctgtcagc tctctggttt ctgatgaaga cttgtgatat
     2521 ctatttattg atttatgatt agtaaattgt ttgcagagaa acggaacttc ccatctgtac
     2581 tgcttttcat gtgggaagtg cacatgcttg agttgagccc ctggggcttc agatgccatc
     2641 tctgtgtggc tcgcagctcg aaaagtagcc caacagttgg actgaatgat tccgtttgtg
     2701 tgctggattc cctcagaatg ctaatgggat ctaagatgaa agaccctctg agcccgtggt
     2761 cctcctcaga gggctccctc actgaggggc caacgtcaga acaagcactg gaagattcta
     2821 atactgccac ctactgctgt gtgcatctgc aactggagac cctcccccgc ctttccttgt
     2881 gcagtgtgct tgacttcctc taccaaaacg gatacctctc acacattttg tgatagggaa
     2941 gccacatttg agctgtgcag tcacgggtct tgttttcact ttatactttg agcccctcag
     3001 tggcaagagg cgagcccttt gtgtaggctc aaaccatgca gttcaaacca tgcagggtag
     3061 ccacaggcat ttgctgcttc gtagttcctg tgtgtggtgc cccgcccttc ctgtgtgtgt
     3121 ggagcttggg ttacagaaac ttcctgacac agcacattgt tgttagtgcc ctgtgtggtg
     3181 cacctgagga agctgttgta ggacctgcag cgtctggggt gctgaaggag cttgcagtgt
     3241 aagaacattg tgggtttcgg ggctggggat ttagctcagt ggtagagcgc ttgcctagga
     3301 agcgcaaggc cctgggttcg gtccccagct ccgaaaaaaa gaaccaaaaa aaaaaaaaaa
     3361 aagaacattg tgggtttcat ctgaggtctg gtttttaacc aaagattagc acataactag
     3421 gttttctatt tctctgaagc agggtcttgg gcaggcaaga tagctctgca agcctggtga
     3481 cctgagtcca gtcactgggc tactgtgatt gtccccgact agtcctgtca aggcgcacac
     3541 atgcccgggg agccaggaac aggcgggcct tgatgagtgt tgggccccgc ttagttgacc
     3601 tccacaccag ctaaggctac ataatgagac cccatctcta taaaagactg ataataaaca
     3661 aatgtggaca ctgaaccact gaaacag
//
