LOCUS       NM_001395101           12269 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus spen family transcriptional repressor (Spen),
            mRNA.
ACCESSION   NM_001395101 XM_039110805
VERSION     NM_001395101.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 12269)
  AUTHORS   Zhang,N., Shi,J., Shi,X., Chen,W. and Liu,J.
  TITLE     Mutational Characterization and Potential Prognostic Biomarkers of
            Chinese Patients with Esophageal Squamous Cell Carcinoma
  JOURNAL   Onco Targets Ther 13, 12797-12809 (2020)
   PUBMED   33363385
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12269)
  AUTHORS   Li,Y., Lv,Y., Cheng,C., Huang,Y., Yang,L., He,J., Tao,X., Hu,Y.,
            Ma,Y., Su,Y., Wu,L., Yu,G., Jiang,Q., Liu,S., Liu,X. and Liu,Z.
  TITLE     SPEN induces miR-4652-3p to target HIPK2 in nasopharyngeal
            carcinoma
  JOURNAL   Cell Death Dis 11 (7), 509 (2020)
   PUBMED   32641685
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 12269)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   4  (bases 1 to 12269)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   5  (bases 1 to 12269)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   6  (bases 1 to 12269)
  AUTHORS   Oswald,F., Winkler,M., Cao,Y., Astrahantseff,K., Bourteele,S.,
            Knochel,W. and Borggrefe,T.
  TITLE     RBP-Jkappa/SHARP recruits CtIP/CtBP corepressors to silence Notch
            target genes
  JOURNAL   Mol Cell Biol 25 (23), 10379-10390 (2005)
   PUBMED   16287852
REFERENCE   7  (bases 1 to 12269)
  AUTHORS   Hiriart,E., Gruffat,H., Buisson,M., Mikaelian,I., Keppler,S.,
            Meresse,P., Mercher,T., Bernard,O.A., Sergeant,A. and Manet,E.
  TITLE     Interaction of the Epstein-Barr virus mRNA export factor EB2 with
            human Spen proteins SHARP, OTT1, and a novel member of the family,
            OTT3, links Spen proteins with splicing regulation and mRNA export
  JOURNAL   J Biol Chem 280 (44), 36935-36945 (2005)
   PUBMED   16129689
REFERENCE   8  (bases 1 to 12269)
  AUTHORS   Yang,X., Li,J., Qin,H., Yang,H., Li,J., Zhou,P., Liang,Y. and
            Han,H.
  TITLE     Mint represses transactivation of the type II collagen gene
            enhancer through interaction with alpha A-crystallin-binding
            protein 1
  JOURNAL   J Biol Chem 280 (19), 18710-18716 (2005)
   PUBMED   15778499
REFERENCE   9  (bases 1 to 12269)
  AUTHORS   Newberry,E.P., Latifi,T. and Towler,D.A.
  TITLE     The RRM domain of MINT, a novel Msx2 binding protein, recognizes
            and regulates the rat osteocalcin promoter
  JOURNAL   Biochemistry 38 (33), 10678-10690 (1999)
   PUBMED   10451362
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Jun 3, 2021 this sequence version replaced XM_039110805.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA5760383, SAMEA5760386
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-420               JAXUCZ010000005.1  159131324-159131743 c
            421-741             JAXUCZ010000005.1  159112866-159113186 c
            742-1218            JAXUCZ010000005.1  159108278-159108754 c
            1219-1379           JAXUCZ010000005.1  159085660-159085820 c
            1380-1580           JAXUCZ010000005.1  159084836-159085036 c
            1581-1732           JAXUCZ010000005.1  159079909-159080060 c
            1733-1858           JAXUCZ010000005.1  159078781-159078906 c
            1859-1972           JAXUCZ010000005.1  159078324-159078437 c
            1973-2086           JAXUCZ010000005.1  159076486-159076599 c
            2087-2187           JAXUCZ010000005.1  159074524-159074624 c
            2188-10246          JAXUCZ010000005.1  159061421-159069479 c
            10247-10747         JAXUCZ010000005.1  159060471-159060971 c
            10748-10942         JAXUCZ010000005.1  159060150-159060344 c
            10943-11101         JAXUCZ010000005.1  159059756-159059914 c
            11102-12269         JAXUCZ010000005.1  159058258-159059425 c
FEATURES             Location/Qualifiers
     source          1..12269
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..12269
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /note="spen family transcriptional repressor"
                     /db_xref="GeneID:690911"
                     /db_xref="RGD:1589867"
     exon            1..420
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     CDS             338..11233
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /note="SPEN homolog, transcriptional regulator;
                     rCG30673-like"
                     /codon_start=1
                     /product="msx2-interacting protein"
                     /protein_id="NP_001382030.1"
                     /db_xref="GeneID:690911"
                     /db_xref="RGD:1589867"
                     /translation="MVRETRHLWVGNLPENVREEKIIEHFKRYGRVESVKILPKRGSE
                     GGVAAFVDFVDIKSAQKAHNSVNKMGDRDLRTDYNEPGTIPSAARGLDDTVSIASRSR
                     EVSGFRGSGGGPAYGPPPSLHAREGRYERRLDGASDNRERAYEHSAYGHHERGTGAFD
                     RTRHYDQDYYRDPRERTLQHGLYYTSRSRSPNRFDAHDPRYEPRAREQFTLPSVVHRD
                     IYRDDLTREVRGRRPERNYQHSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRS
                     RSSSSDSISSSSSSSTDSSDSSSTSSDDSPARSVQSAAVPAPTSQLLSSLEKDEPRKS
                     FGIKVQNLPVRSTDTSLKDGLFHEFKKFGKVTSVQIHGASEERYGLVFFRQQEDQEKA
                     LTASKGKLFFGMQIEVTAWVGPETESENEFRPLDERIDEFHPKATRTLFIGNLEKTTT
                     YHDLRNIFQRFGEIVDIDIKKVNGVPQYAFLQYCDIASVCKAIKKMDGEYLGNNRLKL
                     GFGKSMPTNCVWLDGLSSNVSDQYLTRHFCRYGPVVKVVFDRLKGLALVLYSEIEYAQ
                     AAVRETKGRKIGGNKIKVDFANRESQLAFYHCMEKSGQDMRDFYEMLTERREERRGSY
                     DYSQDRTYYENVRTPGAYPEDSRRDYPARGRELYSEWETYQGEYYESRYYDDPREYRE
                     YRSDPYEQDIREYSYRQRERERERERFESDRDHERRPIERSQSPVHLRRPQSPGVSPA
                     HSERLPSDSERRLYRRSSERSGSCSSVSPPRYDKLEKARLERYTKNEKTDKERIFDAE
                     RVERERRVIRKEKVEKDKPERQKRKGKVHSPSSQSSETEQENDRDQSPEKPRASTKLS
                     RDRTDKEGPAKNRLELVPCVVLTRVKEKEGKVIDHPPPEKLKARLAHDTAKSSVLDQK
                     SQASQVEPPKADSSKVDAARGKALREKAFSSHAEVVDKEGRPKLRKHLKPEQAPELSA
                     LDLEKLEARKRRFADSSLKVEKPRPEVRKTSPEAEDTRALLKKQPDTPRDGILLREGE
                     SERKPVRKEILKRESKKTKLERLNSTLSPKDCQEPAAVSAGSGSRPSSDVHAALGEPV
                     HGSVETQEAQPKKVMPSKPRLKQLQLLDSQGPEKEEVRKNYCRLREDPVEHKAGQEKP
                     HGVNAEEKLGIDIDHTQSYRKQMEQSRRKQQMEMEIAKSEKFGSPKKDVDEYERRSLV
                     HEVGKPPQDVTDDSPPSKKRRTDHVDFDICTKRERNYRSSRQISEDSERTSCSPGVHH
                     GSFHEDDDPIGSPRLMSVKGSPRVDDKGLPYSNVAVREDPFRCTPYDSGKREQAADMA
                     KIKLSVLSSEGEPNRWDPQMKQDPSRFDVSFPNSIIKRDSLRKRCVRDLEPGEVPSDS
                     DEDAEHRAQSPRASSFYDSPRLSFLLRDRDHKLRDRDDRLSSSLERNKFYSFALDKTI
                     TPDTKALLERAKSLSSSREENWSFLDWGSRFANFRDNKDKEKVDSAPRPIPSWYMKKK
                     KIRTDSEGKLDDKKDEHREEEQERQELFASRFLHSSIFEQDSRRLQHLERKSEESDFP
                     PGRLYGRQASEGANSTSDSVQEPVVLFHSRFMELTRMQQKEKEKDQKPKETEKQEGPE
                     TPKTPEPTTETKEPEPKAPVSVALPAITAVTPEPPPPAAAAEKAEETSEAPSLAGERP
                     EEPTPVLEETKLVSEPPSESVEQRKQVDLSPGKDSHDSTAVVASTPPESTTTDTVPCV
                     DAEPLTPGTTVSQVESSADPKPSSPQPPSKLTQRCEEAEEPKVERPETTASIEPDATE
                     KAEVVPEVQPPASEDVEASPPVTAKDRKTTKSKRSKTSVPAAAASIVEKPVTRKSERI
                     DREKMKRSSSPRGEAQKLLELKMEADKVTRTASKSSGGDTEHPEPSLPLSRTRRRNVR
                     SVYATMGDHESRSPAKEPTEQPRVTRKRLERELQEAVVVPATPRRGRPPKTRRRVEEG
                     GEQERKEPADTPRPAEGRRSPRSQKLAATGGPQGKRGRNEHRAEAAEATAQVSAREGN
                     AKARGDREAASETRRDRKDPSTDKDGPDIFPVEVERKPPEKTYKSKRGRARSTRSGMD
                     RAAHLKSLEMARQAADKEAEPVAASPEKNESPLEGSGVSPQLADNPADPDKEAEKESV
                     SASTVSPEARQLTKQMELQQAVENIAKLAEPSAAAADKGTATATSEEPAAEDGHKPAH
                     QASETELAAAIGSIISDSSGEPENFSASPSVPPGSQTHLQEGMEPGPHEATPGILETE
                     AATESSAQQASASDPPAGSADTKEARGNSSDSVQQAKEAKVAVTPPRKDKGRQKTTRS
                     RRKRNVNKKVAAATETRASEAEQPQSESPAAAAAAAAAAAAAAAAAAAEAEVVVAAAP
                     PEEPQEEKQSEKPHSPLRECTSDPSKTPPAESLSQENSVEEKTPCKTPAPPDLPPLSQ
                     PALVDDEPQARFKVHSIIESDPVTPPSDSSIPPPTIPLVTIAKLPPPVIPGGVPHQSP
                     PPKVTEWITRQEEPRAQSAPSPALPPDTKASDVDTSSSTLRKILMDPKYVSATGVTST
                     SVTAAIAEPVSAPRLQEAPAPPCEPKRPSSEEKVAAAVPHADTQASEVPVAADKEKVA
                     PVIAPKITSVISRMPVSIDLENSQKITLAKPAPQTLTGLVSALTGLVNVSLVPVNALK
                     GPVKGSVATLKGLVSTPAGPVNLLKGPVNVLTGPVNVLTTPVSATVGTVSAAPGTVTA
                     ACGVTATTGTAAMSGAVPAPAAKGKQRSSSNENSRFHPGSMSVIDDRPADTGSGAGLR
                     VNTSEGVVLLSYSGQKTEGPQRISAKISQIPPASAMDIEFQQSVSKSQVKADSVTPTQ
                     AAPKGPQTPSAFANVAAHSTLVLTAQTYNASPVISSVKTDRPSLEKPEPIHLSVSTPV
                     TQGGTVKVLTQGINTPPVLVHNQLVLTPSIVTTNKKLADPVTLKIETKVLQPANLGPT
                     LTPHHPPALPSKLPAEVNHVPSGPSNPADRTIAHLATTKPDTHSPRPTGPTPGPFPRA
                     CHPSSTTSTALSTNATVMLAAGIPVPQFISSIHPEQSVIMPPHSITQTVSLGHLSQGE
                     VRMSTPTLPSITYSIRPETLHSPRAPLQPQQIEARAPQRVSTPQPATTAVPALATQHP
                     PEEEVHYHLPVARAAAPVQSEVLVMQSEYRLHPYTVPRDVRIMVHPHVTAVSEQPRAT
                     EGVVKVPPASKAPQQLVKEVVKTSDAKAVPAPAPVPVPVPVPTPAPPPHGEARILTVT
                     PSSQLQGLPLTPPVVVTHGVQIVHSSGELFQEYRYGDIRTYHAPAQLTHTQFPVASSI
                     NLPSRTKTSAQGPPESEPLQSTQPAQPAPSTQSTQPVPPAPPCQPSQLSQPAQPPSGK
                     IPQVSQEAKGTQTGGIEQTRLPAMPTNRPSEPHAQAQRAPGETPQPAHPSPVSVAMKP
                     DLPTPLASQAAPKQPLFVPVNSGTSTPPGLALPHAEAQPAPKQDSAPHGTPQRPVDMV
                     QLLKKYPIVWQGLLALKNDTAAVQLHFVSGNNVLAHRSLPLSEGGPPLRIAQRMRLEA
                     SQLEGVARRMTVETDYCLLLALPCGRDQEDVVSQTESLKAAFITYLQAKQAAGIINVP
                     NPGSNQPAYVLQIFPPCEFSESHLSRLAPDLLASISNISPHLMIVIASV"
     exon            421..741
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            742..1218
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1379
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1580
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1732
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1858
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..1972
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            1973..2086
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2187
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..10246
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            10247..10747
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            10748..10942
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            10943..11101
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
     exon            11102..12269
                     /gene="Spen"
                     /gene_synonym="RGD1564662"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgccggctc cgtcgtagtc gcggccgccc gggtctcgct cgcccctcct cccgctcccc
       61 cgcccgccgc cgcccgcgcg catgcgccgc cggagcgcga gggtcggctt cgggtgtttg
      121 gtggcggcag agcggagctg cgaggcccga gagtcagaac ctgggggaga gggatggtct
      181 ctgcaccggg gggagccgga ggagccgccg ccgctgccga cgccaccgcc gcagccgccg
      241 ccgccgccgc cccggcgccc gcctccctgc gctgacggcc gcgtacgaag ccggcgaggg
      301 ggagccagcc acggcggtcg ccggcacacc gcccagcatg gtccgggaaa ccaggcacct
      361 ctgggtggga aacttaccgg agaacgtgcg agaagagaag atcatcgagc atttcaaacg
      421 atatggccgc gtggaaagtg tcaaaattct ccccaagcgg gggtctgaag gaggagtggc
      481 tgcctttgtg gattttgtgg acatcaaaag tgcacagaaa gctcacaact ccgtcaacaa
      541 aatgggagac agagaccttc gcacggatta taatgaacca ggcactattc cgagtgctgc
      601 tcggggactg gatgatacag tttccatagc atctcgtagt agagaggttt ctgggttcag
      661 aggaagtggt ggagggcctg cttatggccc cccaccatca cttcatgcac gggaaggacg
      721 ctatgagcgg agactcgatg gggcttcaga taacagggag cgtgcttatg aacatagtgc
      781 ctatggacac catgaacggg ggacaggagc atttgatcgg acaagacatt acgatcagga
      841 ttactataga gaccctcgag agcggacttt acaacacggg ctctattaca cttctcggag
      901 tcgaagtcca aaccgctttg atgctcatga cccccgatac gaacctaggg cccgggagca
      961 gtttacactg cccagtgtgg tacacaggga tatctacagg gacgacctta cccgggaggt
     1021 acgaggcaga aggccagagc gcaactacca gcacagcagg agtcgctcac cacattcatc
     1081 ccagtctaga aaccagtccc cgcagaggct ggccagccaa gcgtccagac ccaccagatc
     1141 ccccagcggc agtggctcca ggagcagatc ctccagcagc gactccatca gcagcagcag
     1201 cagcagcagc acagacagta gtgattccag cagcacgtcc agtgacgact ccccagcccg
     1261 ctccgtccag tcagcggcag ttcccgcacc cacttcccag ttgctttcgt ctcttgaaaa
     1321 agatgagccc cggaaaagtt ttgggatcaa agttcagaat cttccagtac gctctacaga
     1381 tacgagcctt aaagatggcc ttttccatga atttaagaag tttggcaagg tgacttcagt
     1441 gcagatccat ggggcctccg aggagaggta cggcctggtg ttcttccggc aacaagagga
     1501 ccaagaaaaa gcactgactg catcaaaggg aaaactcttc tttggcatgc agattgaggt
     1561 gactgcatgg gtaggaccag aaacagagag tgaaaacgag tttcgaccct tggacgagag
     1621 gatagatgaa ttccacccca aagcaacgag aacgcttttt attgggaacc ttgaaaaaac
     1681 gactacttac cacgaccttc gaaacatttt tcagcgcttt ggagaaattg tggacataga
     1741 cattaagaag gtgaacggag tccctcagta tgcgttcctg cagtactgtg acattgccag
     1801 tgtgtgcaag gctatcaaga agatggacgg agagtacctt gggaataacc ggctcaagct
     1861 gggttttgga aagagcatgc ctaccaactg tgtgtggtta gatgggcttt cttcaaacgt
     1921 gtctgatcag tatttaacac ggcatttctg ccgatatggg cctgtggtaa aggtggtgtt
     1981 tgaccgcttg aaaggcctgg ctctggttct ctacagcgag attgagtacg cgcaagccgc
     2041 tgtcagggag accaagggca ggaagattgg tgggaataag attaaggtgg actttgcaaa
     2101 ccgggaaagc caactggcat tttatcactg catggagaag tctggtcaag acatgagaga
     2161 cttctatgaa atgttaacag aaagaagaga ggagcgaaga ggatcctatg actacagtca
     2221 agaccggaca tactatgaaa atgtccgtac tccgggcgcc taccctgagg actccaggcg
     2281 ggactaccca gctcgaggga gagagcttta ctcggagtgg gagacgtacc aaggagagta
     2341 ttacgagtcc cgatactatg atgaccctcg agagtacaga gaatacagga gcgaccctta
     2401 tgaacaagac attcgggaat acagctatag gcagagagag cgggagcgag aacgggagag
     2461 atttgaatct gaccgagacc atgagcggag gcccattgag cgaagtcaga gtcccgtgca
     2521 cttgcgtcgc ccacagagtc ccggggtgtc tcctgcacat tcggagaggt tgccgagtga
     2581 ctctgagagg agactgtacc gccggtcctc agagagaagt ggaagctgca gctcggtgtc
     2641 tcccccacgg tacgataagc tggagaaggc acggttggag cgctatacaa aaaatgaaaa
     2701 gacagataaa gaacgaattt ttgacgctga gcgggtggag agggagaggc gagtaatccg
     2761 caaggagaaa gtagagaagg acaaacccga gaggcagaaa cggaaaggaa aggtgcactc
     2821 ccctagctct cagtcttcag aaacagagca ggagaacgac cgagatcaga gccctgagaa
     2881 accacgggct tccaccaaac tgagcagaga caggactgac aaggaggggc cagccaagaa
     2941 ccgcctggag ctggtgcctt gtgtggtttt gacccgagta aaggaaaagg agggtaaggt
     3001 catcgaccac ccgcccccag agaagctcaa agccaggctt gctcacgaca ctgccaagtc
     3061 ctcagtgctg gaccagaagt cccaggcttc tcaggtggag cctcctaagg cagactcgtc
     3121 taaggtggac gctgccaggg ggaaagcgct gagggaaaag gctttttcaa gccacgcaga
     3181 ggttgtggat aaggagggca ggccaaagct caggaagcac ctcaagcccg aacaggcgcc
     3241 tgagctgagt gccctggacc tggagaagct tgaagcaagg aaaaggcgct ttgcagattc
     3301 cagcctgaaa gtagaaaagc caagaccaga ggtcaggaaa accagccctg aggcagagga
     3361 cactcgagcg ctgttaaaga agcagcctga cacaccgagg gatggtattt tgctgaggga
     3421 aggagagtct gaaaggaagc ctgtgagaaa agaaatcctt aaaagggagt caaaaaaaac
     3481 caagctggag agacttaatt caaccctcag ccccaaagac tgccaggaac ctgctgctgt
     3541 ctctgcgggg tctggctctc ggcctagctc agatgtccac gcagccctgg gagaaccagt
     3601 acatggatct gtggagaccc aggaagccca gcccaaaaag gtcatgccct caaaacctcg
     3661 gctcaaacag ctacagctcc tggatagcca agggcccgag aaggaggaag tgaggaaaaa
     3721 ctactgccgg cttcgcgagg atccagtgga gcacaaagca ggccaggaga agcctcatgg
     3781 agtaaatgct gaggagaaac ttggcattga catcgatcac acgcagagct accgaaagca
     3841 gatggagcag agtcgccgga agcagcagat ggagatggag atagcaaagt ctgagaagtt
     3901 cggcagcccc aagaaagatg tggatgagta tgaaaggcgc agccttgtgc acgaggtggg
     3961 caagccccct caggatgtca ccgacgactc cccgcccagc aaaaagcgaa ggacagatca
     4021 cgtggatttc gatatctgta ccaaaagaga gagaaattac cgaagttcac ggcagatcag
     4081 cgaggactcg gaaagaacca gctgttcccc cggcgtccac cacggctcct ttcatgaaga
     4141 tgatgatccc ataggctcgc ctaggttaat gtcagtgaaa gggtccccta gagtggatga
     4201 caaaggtctc ccctattcta atgtagcagt gagagaagac ccgttcagat gtactcctta
     4261 tgattctggc aagagagaac aggcagcaga catggccaaa ataaagctgt ctgtcttgag
     4321 ttctgaaggt gagccaaacc gttgggaccc tcagatgaaa caggacccca gccgctttga
     4381 tgtcagtttc ccaaacagca taattaaaag agacagcctg cgaaagaggt gtgtgcgcga
     4441 tctggagcct ggcgaggtgc cctctgattc tgacgaggac gccgagcaca gagcccagtc
     4501 acccagagct tccagcttct acgacagccc tcggctgtct tttttattga gggacagaga
     4561 ccataaacta cgtgaccgag atgacagact ctctagttct ttagaaagaa ataaatttta
     4621 ttcttttgca ctggataaga caatcacacc agacactaag gctttgcttg aaagagccaa
     4681 atccctctcg tcctcccgtg aagaaaactg gtctttcctc gactggggct cccgctttgc
     4741 taattttcga gacaacaaag ataaagagaa ggttgactcc gccccaagac cgattccatc
     4801 ctggtacatg aagaagaaga aaatcaggac ggactcagag gggaaactgg acgacaagaa
     4861 ggacgagcac agggaagagg agcaggagcg gcaggagtta tttgcctctc ggttcttaca
     4921 cagctccatc tttgaacaag attccaggcg gctgcagcac cttgagagaa agagcgagga
     4981 gtctgacttc cctcccgggc ggctgtacgg caggcaggca tctgagggtg cgaacagcac
     5041 aagtgactca gtccaggagc cagtggttct gttccatagc agattcatgg agctcactcg
     5101 gatgcagcag aaggagaagg agaaggacca gaagcccaaa gagactgaga aacaggaggg
     5161 tccagagact cccaagactc cagaacccac cactgagact aaagagccgg agcccaaagc
     5221 ccctgtttcc gttgcacttc ctgccatcac cgctgtgacg ccagaaccac cgccaccagc
     5281 agcagcagca gagaaggctg aggaaacatc agaggctcct tcactggcag gagagaggcc
     5341 cgaggagccc actcctgtct tggaagaaac aaagcttgtg tctgagccgc cttctgaatc
     5401 cgtggaacag cgcaagcagg tggacttatc ccctggaaag gacagccatg acagcactgc
     5461 tgtggtagcc tccactcctc cagaaagcac aactactgac actgtccctt gtgtggacgc
     5521 cgagcctctg actcccggaa ccacagtttc ccaggtagag agcagtgcag accccaaacc
     5581 cagcagtccc cagccccctt caaaactgac tcagaggtgt gaagaagctg aagagcctaa
     5641 agtggagagg ccagaaacaa ctgcaagcat tgaacctgac gccactgaga aagccgaagt
     5701 cgtgcctgaa gttcagcccc cagcttctga ggatgtggaa gccagtcctc ccgtcacagc
     5761 aaaagaccgg aagacgacca aaagcaagcg ttccaagaca tcagtcccgg cagctgccgc
     5821 aagcatcgtg gagaagcctg tcaccaggaa gagcgagagg atagaccggg agaagatgaa
     5881 gcggtccagc tctcctcgtg gagaagcaca gaagctgtta gaactcaaga tggaggcaga
     5941 caaggtcacg aggactgcgt ctaaaagctc tgggggcgac acagagcacc ctgagcctag
     6001 cttgcctctc agcagaacca ggcgccgaaa tgtaaggagt gtttacgcca ccatgggtga
     6061 ccacgaaagc cggtctccag ccaaagagcc tactgagcaa cccagagtga ccaggaagag
     6121 attggagcgg gagctgcagg aggctgtggt agttcccgcc acccctcgga ggggaaggcc
     6181 tcctaaaacg cgccggcgtg tcgaagaggg cggtgagcag gaaagaaaag aaccagcaga
     6241 cacacccagg cctgctgagg ggcggaggtc tcccaggtct caaaaattgg cagctacagg
     6301 tgggccccaa gggaaaaggg ggcggaatga gcacagggcc gaggccgctg aggccactgc
     6361 ccaggtgagc gcgagagaag ggaacgctaa ggccaggggc gatagagaag cagcaagtga
     6421 gacaaggcgc gacaggaaag accccagcac agacaaagac gggcctgaca tcttcccagt
     6481 tgaggtagag agaaaacctc cagagaaaac ctacaaatca aagagaggcc gtgctcgtag
     6541 caccaggtca gggatggaca gagctgcaca tctgaaaagt ctggagatgg ccaggcaggc
     6601 agcagacaag gaggcagagc ctgtggccgc ctcccctgag aaaaacgaga gccccctgga
     6661 gggaagtggc gtctctcctc agctggcaga caacccagcc gaccctgaca aggaagcaga
     6721 gaaggaaagt gtgtctgcct ccacggtgtc cccagaagcc aggcagctaa ccaagcagat
     6781 ggagctgcag caggctgtgg agaacattgc aaagctggcc gagccctcag ctgctgcagc
     6841 tgacaagggc acagccacag ccacgtctga agagcccgcc gctgaggatg gccacaagcc
     6901 ggcacaccag gcaagcgaaa ctgaattggc tgcggccatt ggctccatca tcagtgacag
     6961 ctctggagag ccagaaaact tctctgcttc tccctctgtc cccccaggat ctcagacaca
     7021 tctccaggaa ggaatggagc cagggcccca cgaggccaca ccaggaatct tggagacaga
     7081 ggcagcaaca gagtcttctg cccaacaagc cagtgcctcg gacccaccag caggctctgc
     7141 ggacaccaag gaagccagag gaaatagcag tgactctgtg cagcaagcca aggaggctaa
     7201 agtggcagtc actcctcccc ggaaagacaa agggcgccaa aagacgacac gatcacgccg
     7261 gaaaaggaac gttaacaaga aagtggccgc tgccacagag actcgtgcct ctgaggctga
     7321 gcagccacaa agtgagagcc ctgctgctgc tgctgctgct gctgctgccg ccgccgccgc
     7381 cgccgctgcc gccgccgctg aggctgaggt ggtggtggca gcagcacccc cagaagagcc
     7441 ccaggaagaa aagcagagtg agaagcctca ctcccctctc agggagtgca cttctgaccc
     7501 cagcaagacc ccacctgctg agagtctgtc ccaggaaaac agtgttgaag aaaagactcc
     7561 atgcaaaacc cctgcccccc ctgacctgcc ccccctttca cagccagcac tggtggatga
     7621 tgaaccccaa gccaggttca aggtgcattc aatcatcgaa agtgatccag tgaccccacc
     7681 cagtgactcc agcatacctc cacccacaat tcctttagtg accatagcaa agctcccacc
     7741 tcctgtcatc cctgggggag tcccacatca gagtccccct cctaaggtga cagagtggat
     7801 cacaagacag gaagagcctc gggctcagtc tgccccctca ccggctcttc ccccagacac
     7861 aaaggcttct gatgtagaca ccagctccag tacactgagg aagatcctca tggaccccaa
     7921 atatgtgtct gccacaggtg ttacttccac aagtgtcact gcggccattg cagagcctgt
     7981 gagtgcccct cgtctgcagg aggcgccggc ccctccctgt gaacctaagc gcccttcctc
     8041 agaagagaag gtagcagccg cagtaccaca tgccgacaca caggcctcag aggttccagt
     8101 cgctgctgac aaagagaagg tggcccctgt catcgcccct aaaatcacat cagttattag
     8161 ccggatgcct gttagcatcg acctggagaa ctcacagaag ataacgttgg caaagccggc
     8221 tccgcagacc ctgaccggcc tggtgagcgc cctaactggc ctggtgaatg tctctctggt
     8281 cccagtgaat gccttaaagg ggcctgtgaa gggctctgta gccaccctga aaggtttggt
     8341 gagcacccct gctgggcctg tgaatctcct aaaggggccc gtgaatgtcc tcaccggtcc
     8401 tgtcaacgtc ctgaccactc cagtgagtgc cacggtgggc acagtgagcg ctgcccctgg
     8461 taccgtgact gctgcctgtg gtgtgactgc cactacaggc acagcagcca tgtcgggtgc
     8521 agtgcctgcc ccagcagcaa agggcaagca gaggtcaagc agcaacgaga acagccgctt
     8581 ccaccctggg tcaatgtccg tcatcgatga ccgcccagca gacacaggct ctggcgcagg
     8641 gctgcgtgtg aatacttcag aaggggtcgt gctcctgagc tactcaggac agaagacgga
     8701 aggccctcag cgcatcagtg ctaagatcag ccagatccct ccggccagtg caatggacat
     8761 cgagttccag cagtcagtat cgaagtccca ggtgaaagcc gactccgtca ccccgaccca
     8821 ggctgcaccc aaaggccctc agaccccttc tgcctttgca aacgtggctg cccactctac
     8881 cctggtcctg actgcccaga catacaatgc ctctcccgtg atctcctctg tgaagaccga
     8941 ccgtccatcc ttggagaagc ccgagcccat tcacctctcc gtgtccaccc ctgtcaccca
     9001 ggggggcaca gtgaaggttc tcacccaggg catcaacaca cccccagtgc tggttcacaa
     9061 ccagcttgtc ctcaccccaa gcatcgtcac cactaacaag aaacttgctg accctgtcac
     9121 tctgaaaata gagaccaagg tccttcagcc ggctaacctg gggcccaccc tcactcccca
     9181 ccacccccct gcccttccca gcaaactgcc ggccgaagtg aaccatgttc cttccggacc
     9241 cagcaaccca gcagatcgaa ccattgccca cttggcaacc acaaaaccag atactcattc
     9301 tcctcgaccc actgggccta caccaggccc attcccaagg gcctgccatc ccagcagcac
     9361 gacatccaca gcgctttcca ccaatgcaac tgtcatgctg gctgcaggca ttccagtgcc
     9421 acagttcatc tccagcatac acccagagca gtctgtcatc atgccccccc acagcatcac
     9481 tcagaccgtg tctctcggtc acctgtccca aggcgaggtg agaatgagca cacccacctt
     9541 gcccagcatc acctacagca tccggccaga gaccctgcac tctcctcggg cccctctgca
     9601 gccccaacag atagaagcaa gggccccaca gcgtgtgagc acgccccagc cagccacaac
     9661 tgctgtgccc gcgctggcca cacagcaccc tcccgaggaa gaagttcact accacctccc
     9721 tgtggctcga gctgcagccc ctgtgcagtc agaagtgctg gtcatgcagt ccgagtaccg
     9781 gttacaccca tacaccgtgc cccgagacgt ccggatcatg gtgcatccac atgtgactgc
     9841 tgtcagtgaa cagcccaggg ccacagaggg cgtggtgaag gtgccaccag ccagcaaggc
     9901 cccccagcaa ctggtgaaag aagtggttaa gacttcagat gccaaagcag tcccagctcc
     9961 agcccccgtc ccggtccctg tccctgtccc gacccctgcc ccaccccctc atggtgaggc
    10021 ccgaattctc acagtcaccc ccagcagcca gcttcagggg ctgcctctga ccccgcctgt
    10081 ggtggtaacc cacggggtgc agattgtgca ctccagcggg gagctgttcc aagagtatag
    10141 gtatggcgac atccgtacct accacgcccc tgctcagctc acacatactc agtttcctgt
    10201 cgcctcttcc atcaacctac cctccaggac caagacctcg gcccagggcc cccccgaaag
    10261 tgagcccttg cagtctacgc agcctgcaca gcctgcccca tctacgcagt ccacacagcc
    10321 tgtaccacct gccccaccct gccagccctc ccagctcagc cagcctgccc agccaccaag
    10381 tggcaagatt cctcaggtct cccaagaagc caaggggaca cagacaggag ggatagagca
    10441 gactcgcctc ccagccatgc ccacgaacag gccgtctgag ccacatgctc aggctcagag
    10501 ggcaccgggg gaaacccccc aaccagccca tccctccccc gtgtcagtcg ccatgaagcc
    10561 ggacctccca acccctcttg cctctcaggc tgccccaaag cagccattgt ttgtccccgt
    10621 aaactcaggc acgagcaccc cacccggact ggctctgcct catgctgaag cccagccagc
    10681 cccaaagcaa gactctgctc cacacgggac accacaaagg cccgtggata tggtccagct
    10741 tctgaagaag taccccatcg tgtggcaggg cctgctggcc ctcaagaacg acacagctgc
    10801 tgtgcagctc cactttgtct ctggcaacaa cgtcctggct caccgctccc tgcccctctc
    10861 tgaaggcggc cccccgctgc ggatcgccca aaggatgcga ctggaggcct cgcaactgga
    10921 aggagttgcc aggaggatga cagtggaaac agactactgc ctgttgctgg ctctgccctg
    10981 tggccgtgac caggaggatg tcgtaagcca gaccgagtcc ctcaaggctg cctttatcac
    11041 ttacctgcag gccaagcaag cagcagggat tatcaatgtc cccaatcctg gctccaatca
    11101 gcccgcctat gtgctgcaga tcttcccacc ctgtgagttc tccgagagcc acctctcccg
    11161 gctggcccct gacctccttg ccagcatctc caacatctct ccccacctca tgattgtcat
    11221 tgcctcagtg tgaggctctg aatggtcgcc acctcagggc gttttcctga ggctctgctg
    11281 caaaaaaata aataaataaa taaaataaaa taaaaaaatg aacacaggac agaaccatgg
    11341 acaacccagc caggcagagg ggaagctgct gaggggcaga ctgccaggcg ccaccagctg
    11401 ccctcgccct cgtgcccgcc cagctctatg gatcgacctc ctctgggggt ggtgctgctt
    11461 gtatgtttac atgaaattct cgcccaggac agaccaccga cccatggact cgcagaaacc
    11521 ttaggggctg ggtttctctc ctcacctgga gaaaaggaac cgggctgtgg gattcttctg
    11581 ttttgttaaa cagaatggat cagcctttgc actaagttag tgactcgcaa gtcaacacgg
    11641 gctgcgatgc tctggccgtg tacttgagca cgccgtggac tcatgcagga cttgggactt
    11701 gtgctgaaac cttggggtca ggaacatttc attgggtttt tgccccatcc cctcctcctc
    11761 ccttaccctg ctcatcagac agtcatcttt cttaattctc ctgccgccgc cgccgccgcc
    11821 accttcaact caccggatgt acagtttaca gttgagtaaa agcgaacaga aggattttct
    11881 ttcttggtcg gatgtgcaga acttggggtg tgtatatata aatatataat atatataaat
    11941 atatataata ctgacttaaa tcaaatttcc ctgacataca ttttttttaa tctgtgccaa
    12001 aaatgtgttt tcaaagaaaa tcttattttc atactcagac tttgtaccgt cactcatttg
    12061 tataagtgcg tcgtcactgc acgggtcctg tcctgcaaca tagaagagtc acagcacctg
    12121 tacaaagacc tggccactcc actccctctc ctgtgaccgg acctctccct gactcctaac
    12181 acttattaat ttatgaaact gttttcctca gcgcagtttt gttctgtgtg tccattggat
    12241 tacaaacttt attaaaaaat acaaaacac
//
