LOCUS       XM_039103071            5578 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus sperm flagellar 2 (Spef2), transcript
            variant X3, mRNA.
ACCESSION   XM_039103071
VERSION     XM_039103071.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051337.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..5578
                     /gene="Spef2"
                     /gene_synonym="Kpl2"
                     /note="sperm flagellar 2; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 1 EST,
                     2 Proteins, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments, including 11 samples with
                     support for all annotated introns"
                     /db_xref="GeneID:64555"
                     /db_xref="RGD:620450"
     CDS             13..5523
                     /gene="Spef2"
                     /gene_synonym="Kpl2"
                     /codon_start=1
                     /product="sperm flagellar protein 2 isoform X3"
                     /protein_id="XP_038958999.1"
                     /db_xref="GeneID:64555"
                     /db_xref="RGD:620450"
                     /translation="MSFLSNWLPWATGRRSPADCSWKVPSTCKQSNAPVSYVRDPVPV
                     AQPGVEGVSDRPKSFAKTFSNGYLIGEVLFKFELQSDFSEFSESRVSTAKLNNFSRLQ
                     PTLHLLGVQFDQNVAQSIITEKPGAATKLLYQLYIALQKKKKTGLTGLEIQTMQPQTN
                     QRLQALKSEAFRERLKNLIPRQTDFNLMRVTCRFQEKCKQMKEDLARMHFEKFEKIQK
                     LEEEQRHFNIEKQRLNRRRQNEIMAKIQAAIIQIPKPEPNRTLKAIEAQKLMKKKKEA
                     EDVANEIKKFEALIKKDLQTRESISKTSLDTIDQTAAELLNTYSDDDYIKKIQKRLEE
                     DAFAREQREKRRRRLLMDQLIAHEAQEEAYREEQLIHRLMRQSQQERRIAVQLMHVRH
                     EKEVLWQNRIFREKQFEERRLKDFQDALDREAALTKQAKIDFAEQTLRAKEIHEKIAM
                     ERAQQRYEKHYGICAEILDQILDLCTKVADYRLLTNNLIPHKMMRDWKELFFSGNPIY
                     EQTSHTHGQTESTEDHHVELNKRNLLDSKDYEDYKNMTGEWALPEDMANSSPPSNNNI
                     LGHVLLRLIENSYPSEKEVADTELPSFAVKGCLLGKTLSGKTTILKALQNDIPIQVLS
                     IDTLVQEAIQAFNDGEKSSKAPSMHEDVKGDPEVDQKEMIIPQDKNELTDTQVPGEAT
                     PQKEGTKSSDFEQFRPSNSFLSLSMRAQLGAKSELMLRRGKSVPDVLLVNILVNAIKK
                     IPVNQSWVLDGFPMTVNQAKLLEEALTGYKRKSLQLKKKKAQMPTLALSPTTSKEVPL
                     LPSAFDFAILLDISDNSSLARINDIIAKEISHEISHENVSGPTITSSEEKSEDRNLRD
                     HIQHRIVGFLDNWPLLEEWFTEPRNILKKVNAEIDEALLCQKIKEIFATEVSNKKIKV
                     EKKLEEKETEKKSAVSPTEPPTPTPPLASEAEKEKEVHQAKPSGKGKTQSVSPKGKAQ
                     GGKISVKKSPIDSTEVSPTLIAMPTTKPGSEEWVYVTEPIPEELPSFLVPYWELIEKT
                     YISHIKTVLRQLRESQHTVLSYLYETRTSFQEFLRRPDHKQDFVSQWQADFNSVPEDL
                     WEDEETKAELHQRVNDLRDRLWDICEARKEEAEQERLDIINESWLQDYIGITMNHFFS
                     LMQAEVNRFQDTKRLLQDYYRAMESKIPLEDNKKFTRIPLVQLDGKEISEHQLRIPLV
                     PRISSSPENVSMRPKVKALLKGRFDPPLESLEANFEGDEKIVLDTWQQASLAISQMVA
                     AEVHQRLAEEEKEQPQLDPKEKSPQSGANKKAKKEKEQAKKEKEQAKKEKEQAKKEKE
                     PPKKKMAEKKGKGKSSPVVEVSPVTITPEEMAEMERKNELKLKIKEEHLAALQTEEQA
                     TQFRLELIKSKALSILEDLVTKVIEVYRFMEKWLGKRYLNEMASIQKLTEVARYHIET
                     ATKIQNEIYLSQEDFYINGDIKVFPDPPPPTRPPPVEKEENGTLTIEQLDNLRDQFLD
                     MAPKGIIGNKAFTDILLDLVTLNLGTNNLPSSWMHLTQLDLQEITSLLTVNTEFVDWR
                     KFLMVAAMPWPIPLEEELLETLQRFKATDEAQTGTITFEQYKQAGLWFSGDEDIKIPE
                     NPLEPLPFNRQEHLIEFFFRLFADSEKDPPQLDYTQMLLYFACHPDTLEGVYRALSVA
                     VGTHIFRQVETPMLTAEKTSISSVSPNEEFPEAEENSTREELKEEKDERDQKEEEIPE
                     NANTEKISMETLLKVFGGGNEVLDANRFASYLKTENIYSENLIKTFQDLGAKNLEPIE
                     VNILLKHPYIQDLITNYVDYKIPDIKMILQRSEHAQGSDGERSPSRLTDEKK"
ORIGIN      
        1 aactccgcct ggatgagctt ccttagcaac tggttgccct gggcgacggg aagacgcagt
       61 ccagctgact gttcctggaa agttcctagc acctgcaagc agtcaaatgc gccagtgagc
      121 tatgtcagag atcctgtgcc agtggctcaa ccaggagttg aaggtgtctc ggaccgtccc
      181 aagtcatttg caaagacatt ttctaatggc tatctaattg gagaagttct gttcaagttt
      241 gaacttcaga gtgatttttc agagttttca gagagcaggg tctcaactgc caaacttaat
      301 aatttctccc gcttgcaacc aacgcttcac ctgttgggtg tgcagtttga tcagaatgtg
      361 gctcagagca tcatcacaga aaagcccggg gcagcaacca aacttctgta tcagttgtac
      421 attgctcttc agaaaaagaa gaaaactggg ctgactggat tagagataca aaccatgcaa
      481 ccccaaacaa accagcggct tcaggccctc aagagtgagg cttttcgaga gagacttaaa
      541 aacctgatac ctcgccaaac cgatttcaat ctgatgcggg ttacatgcag gtttcaagaa
      601 aagtgtaaac aaatgaaaga agatcttgcc cgaatgcatt ttgagaagtt tgaaaaaatt
      661 caaaaacttg aggaagagca aagacacttt aatatcgaaa agcaacgctt aaacagaaga
      721 cgacaaaatg agataatggc caaaatccag gcagccatta tacagattcc caagcctgaa
      781 cccaaccgta ccttgaaagc tattgaggct caaaaactga tgaaaaagaa aaaggaggca
      841 gaggacgtgg ccaatgagat taagaaattt gaagcactaa ttaaaaagga tctccaaact
      901 agagaaagta tatccaagac ttctttagat acaatagacc agacagctgc ggaactgctc
      961 aacacttact cagatgacga ctacattaaa aagatccaga aacgcctaga agaagatgct
     1021 tttgcacgtg aacaaaggga aaaaagacga cggaggttgt tgatggacca gctgatagcc
     1081 catgaagcac aggaggaggc ctatcgggaa gagcagctca ttcaccggct catgcgccag
     1141 tcccagcagg agcgcaggat tgctgtgcag ctcatgcacg ttcgccacga gaaggaagtt
     1201 ttgtggcaaa atagaatttt cagagagaaa caatttgaag aaagacgact taaggatttc
     1261 caggatgctc tagatagaga agcggctctc accaaacaag ccaagattga ttttgcagag
     1321 caaaccttga gggcgaagga aattcatgaa aagatcgcca tggaaagagc ccaacagcgt
     1381 tacgagaagc attatgggat atgtgcagag attttggatc aaatacttga tttgtgcacg
     1441 aaggtagcag actataggtt gctgacaaat aatcttattc cacacaagat gatgcgtgat
     1501 tggaaagaac tgttttttag tggaaatccc atatatgaac agacttctca tacacatgga
     1561 caaacagaat ccactgagga tcaccatgta gagttgaaca aaaggaactt actcgatagc
     1621 aaagattatg aagactataa gaacatgact ggagagtggg ccttaccaga ggacatggca
     1681 aacagttcac caccctccaa caataacata ctgggtcatg tgcttctgag attaattgaa
     1741 aactcgtatc cgtctgagaa agaagttgca gacactgaat taccttcatt tgctgtgaag
     1801 ggttgtttac tggggaagac actcagtgga aaaactacaa tcctcaaggc tcttcaaaat
     1861 gacatcccta ttcaagtact ttctattgat actcttgtcc aagaggctat ccaggcattt
     1921 aatgatggag aaaaaagtag taaggcacca tcgatgcatg aagatgttaa aggagatcct
     1981 gaagtggacc agaaagaaat gatcatacct caggataaaa atgagctaac agatactcaa
     2041 gttccagggg aagcaacacc ccaaaaagaa ggtacgaaga gtagtgactt tgaacaattc
     2101 agaccaagta atagctttct gtcacttagc atgcgagctc aacttggtgc aaaatcagaa
     2161 ctgatgctca gaagaggcaa gagcgttcca gatgtactgc tggttaacat cctggtaaat
     2221 gctattaaga agatacctgt gaaccaaagc tgggttctag atggatttcc aatgacagta
     2281 aatcaagcca agctcctgga ggaagctctt acaggataca aaagaaaatc cctgcagtta
     2341 aagaagaaaa aagcacaaat gcccacattg gccttgagcc ccacaacttc caaagaggtc
     2401 cctcttctcc catctgcgtt cgattttgcc atattgcttg atatttcaga taactcatca
     2461 ctggctcgca ttaacgatat catagctaaa gaaatctcac atgaaatttc tcatgaaaat
     2521 gttagcggtc ctacaattac ttcaagtgaa gaaaagagtg aggaccggaa cttaagagac
     2581 cacatacagc acagaattgt tggcttcttg gacaactggc cattgttgga agagtggttt
     2641 acagagccaa gaaacatttt gaaaaaagtc aatgctgaaa tagatgaagc gctcttatgc
     2701 caaaaaataa aagaaatttt tgctactgaa gtatcaaata aaaaaatcaa agttgaaaag
     2761 aaactagaag aaaaggaaac tgagaaaaag tcagcagttt ctccgactga gcctcctacg
     2821 ccgacccctc cccttgcttc tgaagcagaa aaagaaaagg aggttcatca ggcgaaacct
     2881 tctgggaaag gaaagacaca atcagtttct cctaaaggaa aagcacaagg aggcaaaatt
     2941 tccgtaaaga aatcacccat tgactcaaca gaagtatcac ccacactaat agcaatgccg
     3001 acaactaagc caggatcaga agaatgggtc tatgtgactg agcctatacc tgaggagcta
     3061 ccttcgtttt tagtacctta ctgggaactg atagagaaaa cctacataag ccacatcaaa
     3121 acagtgcttc gacagctcag agaaagccaa cacaccgtgc tctcttattt atatgagact
     3181 aggacaagtt ttcaggagtt tctaaggcgt ccagatcaca agcaagattt tgtatctcag
     3241 tggcaggctg atttcaactc agttcctgaa gacctgtggg aagatgagga aacaaaggct
     3301 gaactacacc aaagagtaaa tgatctgcga gaccgcctgt gggacatctg tgaagcgagg
     3361 aaggaagagg cagagcagga gagacttgat atcattaacg agagctggct gcaggactat
     3421 atcggaataa caatgaatca cttcttttcc ctgatgcagg cagaagtgaa ccgtttccaa
     3481 gataccaaaa gactccttca agattattac agagcaatgg aatccaaaat cccactggaa
     3541 gacaataaga aatttactcg aatcccattg gttcaactcg atggtaaaga aatttcagaa
     3601 caccagctca gaattcctct agttcctcga atatccagtt ctccagaaaa tgttagcatg
     3661 agaccaaagg tgaaagcatt actgaagggc agattcgatc caccactgga aagtctggaa
     3721 gccaactttg aaggagatga gaagatagta ttagacacct ggcagcaggc ttccttagcc
     3781 atctcccaaa tggtggctgc tgaagttcat caaaggctcg cagaggaaga aaaggaacaa
     3841 ccccaactag atcccaaaga aaagtctcct cagtcgggtg caaacaaaaa ggccaagaag
     3901 gagaaagaac aggccaagaa ggagaaagaa caggccaaaa aggagaaaga acaggccaag
     3961 aaggagaaag aaccgcccaa gaagaaaatg gcagaaaaga aaggaaaagg taaatcatcc
     4021 cctgtggtag aagtcagtcc cgtcacaata acgcccgagg aaatggctga aatggaaagg
     4081 aagaatgagc tgaagctcaa gataaaggaa gaacatcttg ccgccctgca gacagaagaa
     4141 caagccacac aatttcgact tgaactaata aagtcaaaag ctttgtccat cctggaagat
     4201 ttagtaacaa aggtgattga ggtttacagg ttcatggaaa aatggcttgg taagagatat
     4261 ttgaatgaaa tggccagtat acagaaattg accgaagtag ctcgctacca cattgagaca
     4321 gctacgaaaa ttcaaaatga gatttattta agccaagaag acttctacat caatggagac
     4381 ataaaagtct tccctgaccc tcctccacca acccgcccac caccggtaga aaaggaagaa
     4441 aatggcaccc taacaatcga acagcttgat aaccttcgag atcagttctt agatatggcg
     4501 cctaaaggca taataggaaa taaagctttc actgacattc tgcttgatct ggttaccctg
     4561 aacctgggaa caaacaactt gcctagcagc tggatgcacc tcacccaact ggatttgcag
     4621 gagataacat ctttattaac agtcaacaca gagttcgtgg actggaggaa gttcttgatg
     4681 gtagcagcca tgccatggcc catccccctg gaggaggagc tcctggagac cctgcagagg
     4741 tttaaggcca ccgatgaagc tcagacaggc accatcacct ttgaacaata taagcaggct
     4801 ggtctatggt tttcaggaga tgaagatata aaaattccag aaaatcccct tgaacccttg
     4861 ccattcaata gacaggagca tctcatagag ttcttcttca ggctgtttgc tgactctgag
     4921 aaggatccac ctcagcttga ctacactcag atgttgctgt attttgcctg ccacccagac
     4981 actttggaag gagtctacag ggccctcagt gtggctgtgg ggactcacat attccggcag
     5041 gttgaaactc caatgctaac tgcagaaaag acctccatct cctctgtgag tcccaatgag
     5101 gaattccctg aagctgaaga aaacagtaca agagaggagc tgaaggagga gaaagatgag
     5161 agggaccaaa aggaagaaga aatcccagaa aatgcaaaca ctgaaaagat ttccatggaa
     5221 acactgctca aagtgtttgg aggaggaaat gaagtgctgg acgctaacag atttgccagc
     5281 tacctaaaga cagagaatat ttactcagag aacctcatca agacgttcca ggacctgggt
     5341 gccaagaacc tagagccaat cgaagttaat attctcttga agcatcctta cattcaggac
     5401 ctgattacga attatgtaga ctataaaatc cctgacatta aaatgatcct tcaaaggagt
     5461 gagcatgcac aaggcagtga tggagaaagg tcaccctcaa gacttacaga cgaaaagaag
     5521 tgaagactgt ggtttatttt tttattttat tgaataaata ttcataatcg aatgacca
//
