LOCUS       NM_001039024            1527 bp    mRNA    linear   ROD 05-APR-2023
DEFINITION  Rattus norvegicus sperm flagellar 1 (Spef1), mRNA.
ACCESSION   NM_001039024 XM_230612
VERSION     NM_001039024.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Tapia R, Perez-Yepez EA, Carlino MJ, Karandikar UC, Kralicek SE,
            Estes MK and Hecht GA.
  TITLE     Sperm Flagellar 1 Binds Actin in Intestinal Epithelial Cells and
            Contributes to Formation of Filopodia and Lamellipodia
  JOURNAL   Gastroenterology 157 (6), 1544-1555 (2019)
   PUBMED   31473225
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Dougherty GW, Adler HJ, Rzadzinska A, Gimona M, Tomita Y, Lattig
            MC, Merritt RC Jr and Kachar B.
  TITLE     CLAMP, a novel microtubule-associated protein with EB-type calponin
            homology
  JOURNAL   Cell Motil Cytoskeleton 62 (3), 141-156 (2005)
   PUBMED   16206169
  REMARK    Erratum:[Cell Motil Cytoskeleton. 2005 Dec;62(4):259]
REFERENCE   3  (bases 1 to 1527)
  AUTHORS   Chan SW, Fowler KJ, Choo KH and Kalitsis P.
  TITLE     Spef1, a conserved novel testis protein found in mouse sperm
            flagella
  JOURNAL   Gene 353 (2), 189-199 (2005)
   PUBMED   15979255
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC099127.1.
            
            On Oct 8, 2011 this sequence version replaced NM_001039024.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignment to the mouse ortholog, Spef1, GeneID
            70997.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760383,
                              SAMEA5760389 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-612               BC099127.1         1-612
            613-1527            BC099127.1         795-1709
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q36"
     gene            1..1527
                     /gene="Spef1"
                     /gene_synonym="RGD1311267"
                     /note="sperm flagellar 1"
                     /db_xref="GeneID:311429"
                     /db_xref="RGD:1311267"
     exon            1..251
                     /gene="Spef1"
                     /gene_synonym="RGD1311267"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..844
                     /gene="Spef1"
                     /gene_synonym="RGD1311267"
                     /codon_start=1
                     /product="sperm flagellar protein 1"
                     /protein_id="NP_001034113.2"
                     /db_xref="GeneID:311429"
                     /db_xref="RGD:1311267"
                     /translation="MESSVDEEALHQLYLWVDNIPLSRPKRNLSRDFSDGVLVAELIK
                     FYFPKMVEMHNYVPANSLQQKLSNWGHLNRKVLNKLNFSVPDDVMRKIAQCSPGVVEL
                     VLIPLRQRLEERQRRQKLGVGSLQELVPQDSSGYMDMGLSQKVRGDAPTLGEQLREGH
                     PLAPRPPGYNQALQGDPNFVLQIAEKEQELLASQETVQVLQMKVKRLEHLLQLKNVRI
                     DDLSRRLQQAERKQR"
     exon            252..363
                     /gene="Spef1"
                     /gene_synonym="RGD1311267"
                     /inference="alignment:Splign:2.1.0"
     exon            364..520
                     /gene="Spef1"
                     /gene_synonym="RGD1311267"
                     /inference="alignment:Splign:2.1.0"
     exon            521..560
                     /gene="Spef1"
                     /gene_synonym="RGD1311267"
                     /inference="alignment:Splign:2.1.0"
     exon            561..612
                     /gene="Spef1"
                     /gene_synonym="RGD1311267"
                     /inference="alignment:Splign:2.1.0"
     exon            613..736
                     /gene="Spef1"
                     /gene_synonym="RGD1311267"
                     /inference="alignment:Splign:2.1.0"
     exon            737..1502
                     /gene="Spef1"
                     /gene_synonym="RGD1311267"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctcccagatg ggatttgagt ctgtgcggaa taaggaccca aacacctatc tgaacgctcg
       61 caattgcgag aggaggcatt tgaagggttc tggggctagc gctgggctct gtcgggaacg
      121 gtgaggcctt gctctggatc taatggaaag cagcgtggat gaggaggctc tgcaccagct
      181 gtacctctgg gtagacaaca tccctctgtc cagacccaag aggaacctct cccgggattt
      241 cagcgacgga gtcctggttg cagagcttat caagttttac ttccccaaga tggtggagat
      301 gcacaattat gtccccgcca actctctcca gcagaagctc agcaactggg gtcacctgaa
      361 cagaaaggtg ctgaacaagc tgaacttctc cgtcccagat gatgtgatgc ggaaaattgc
      421 gcagtgttct ccgggggtag tggagctagt gctcattcca ttgaggcagc gcctggagga
      481 gcggcagagg cgccagaagc tgggtgttgg ctccttacag gagctggttc ctcaggatag
      541 cagtggctac atggatatgg gtttgtccca gaaggttcga ggagatgccc caaccctggg
      601 agagcagctc agagagggcc atccgctggc tccccgaccc cctgggtata accaggcgct
      661 gcagggggat ccgaacttcg tcctccagat cgctgaaaag gagcaggagc tgttggcctc
      721 tcaagagaca gtacaggtcc ttcagatgaa ggtgaagcgt ctggaacatc tgctccaact
      781 caagaacgtg cgcatcgatg acctctcccg gcggctccag caggccgagc gcaagcagcg
      841 gtgagcggtg ccagggctgc gcggggtttc ccaatacccg ccagagccca gacgccgcga
      901 cccaccactc acctgcggac gcaggattgg gagggcggag cgagctgctc gttagcgtct
      961 ccggcagcta ccgcgctcgg ctgcaaaggg ttcgggcgaa cgtgggggtg ttcaaaaccg
     1021 aggccggaca ctgatcctcc tccttacact ccatctgggc caggaaaaag gaccactttc
     1081 cacagcccag aaaacctgta cgaacacacc tcatgacttc ccaaatcgtg tcctctttga
     1141 agctcccggc actgggcctg gagaggcggt cctctggctc gttccacacc cctagaaccg
     1201 gttcctttct cagcatagaa gatagcttca tctctgtcca tgctaaatct ccatgccccc
     1261 cgacacgcga gaaactatcc ccggaagctg aggaattctc ttgccccttc tgggtgctga
     1321 cctgggatcc ttctcagggc tggaaatcac agcagctggc gaggaaggga taaggtgttg
     1381 aagtagtcgg gagtaaggga ggcatccagc tgtgcaatcg ctctcctctc agggagcatt
     1441 atcaggagtg cctggggaag acaaggcaga gctgtctcca ttaaaggtgg tgttgtgcgt
     1501 tcaaaaaaaa aaaaaaaaaa aaaaaaa
//
