LOCUS       NM_001131006             722 bp    mRNA    linear   ROD 20-MAR-2023
DEFINITION  Rattus norvegicus signal peptidase complex subunit 1 (Spcs1), mRNA.
ACCESSION   NM_001131006
VERSION     NM_001131006.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Ma L, Li F, Zhang JW, Li W, Zhao DM, Wang H, Hua RH and Bu ZG.
  TITLE     Host Factor SPCS1 Regulates the Replication of Japanese
            Encephalitis Virus through Interactions with Transmembrane Domains
            of NS2B
  JOURNAL   J Virol 92 (12), e00197-18 (2018)
   PUBMED   29593046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  AUTHORS   Zhang,R., Miner,J.J., Gorman,M.J., Rausch,K., Ramage,H.,
            White,J.P., Zuiani,A., Zhang,P., Fernandez,E., Zhang,Q., Dowd,K.A.,
            Pierson,T.C., Cherry,S. and Diamond,M.S.
  TITLE     A CRISPR screen defines a signal peptide processing pathway
            required by flaviviruses
  JOURNAL   Nature 535 (7610), 164-168 (2016)
   PUBMED   27383988
REFERENCE   3  (bases 1 to 722)
  AUTHORS   Suzuki R, Matsuda M, Watashi K, Aizaki H, Matsuura Y, Wakita T and
            Suzuki T.
  TITLE     Signal peptidase complex subunit 1 participates in the assembly of
            hepatitis C virus through an interaction with E2 and NS2
  JOURNAL   PLoS Pathog 9 (8), e1003589 (2013)
   PUBMED   24009510
REFERENCE   4  (bases 1 to 722)
  AUTHORS   Hori O, Miyazaki M, Tamatani T, Ozawa K, Takano K, Okabe M, Ikawa
            M, Hartmann E, Mai P, Stern DM, Kitao Y and Ogawa S.
  TITLE     Deletion of SERP1/RAMP4, a component of the endoplasmic reticulum
            (ER) translocation sites, leads to ER stress
  JOURNAL   Mol Cell Biol 26 (11), 4257-4267 (2006)
   PUBMED   16705175
REFERENCE   5  (bases 1 to 722)
  AUTHORS   Farkas RH, Qian J, Goldberg JL, Quigley HA and Zack DJ.
  TITLE     Gene expression profiling of purified rat retinal ganglion cells
  JOURNAL   Invest Ophthalmol Vis Sci 45 (8), 2503-2513 (2004)
   PUBMED   15277470
REFERENCE   6  (bases 1 to 722)
  AUTHORS   Kalies KU and Hartmann E.
  TITLE     Membrane topology of the 12- and the 25-kDa subunits of the
            mammalian signal peptidase complex
  JOURNAL   J Biol Chem 271 (7), 3925-3929 (1996)
   PUBMED   8632014
REFERENCE   7  (bases 1 to 722)
  AUTHORS   Shelness GS, Lin L and Nicchitta CV.
  TITLE     Membrane topology and biogenesis of eukaryotic signal peptidase
  JOURNAL   J Biol Chem 268 (7), 5201-5208 (1993)
   PUBMED   8444896
REFERENCE   8  (bases 1 to 722)
  AUTHORS   Evans,E.A., Gilmore,R. and Blobel,G.
  TITLE     Purification of microsomal signal peptidase as a complex
  JOURNAL   Proc Natl Acad Sci U S A 83 (3), 581-585 (1986)
   PUBMED   3511473
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF976587.1 and CV795143.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC166925.1, CF976588.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                CF976587.1         5-27
            24-722              CV795143.1         1-699               c
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16p16"
     gene            1..722
                     /gene="Spcs1"
                     /gene_synonym="RGD1309125"
                     /note="signal peptidase complex subunit 1"
                     /db_xref="GeneID:290555"
                     /db_xref="RGD:1309125"
     exon            1..168
                     /gene="Spcs1"
                     /gene_synonym="RGD1309125"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..441
                     /gene="Spcs1"
                     /gene_synonym="RGD1309125"
                     /EC_number="3.4.-.-"
                     /note="signal peptidase complex subunit 1 homolog"
                     /codon_start=1
                     /product="signal peptidase complex subunit 1"
                     /protein_id="NP_001124478.1"
                     /db_xref="GeneID:290555"
                     /db_xref="RGD:1309125"
                     /translation="MLEHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQ
                     FGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQDSGTEDKKSGDRKIKRHAKNN
                     "
     exon            169..228
                     /gene="Spcs1"
                     /gene_synonym="RGD1309125"
                     /inference="alignment:Splign:2.1.0"
     exon            229..315
                     /gene="Spcs1"
                     /gene_synonym="RGD1309125"
                     /inference="alignment:Splign:2.1.0"
     exon            316..705
                     /gene="Spcs1"
                     /gene_synonym="RGD1309125"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcttccgggg ccacagccga agagaggcgc ttggctggtc ggcctggctg ctttcggtat
       61 cgcacttcgc aagccacact ccttaatagt cagctacctt cgtttcactg tcggtatcag
      121 tcttcggcaa ccatgctgga acacctgagc tcgctgccca cccagatgga ttacaagggg
      181 cagaagctag ctgaacagat gtttcaggga attattcttt tttctgcaat agttggattt
      241 atctacgggt atgtggctga acagttcggg tggaccgtct acatagttat ggctgggttt
      301 gctttctcgt gtttgctgac acttcctccg tggcccatct accgccgcca tcccctgaag
      361 tggttacctg ttcaagactc aggcacagaa gacaagaaat caggggacag aaaaattaag
      421 aggcatgcta aaaataactg attgggactt tcacgattca gcacctgctt tttgtttcct
      481 gtaagatggc tggattgctt tcataaccaa aacagcttaa accacctatg ttcttacagc
      541 acagctgtgt ggagatgtag ttgtcctggc ttcctttcta accggcatta ttttaagtca
      601 taactatttt atactgttct tcgtaagctt gttttggaga accggacata caggtatatg
      661 tcgcttcttt catgtgtgca gtgaataaac acctcataac tcctaaaaaa aaaaaaaaaa
      721 aa
//
