LOCUS       NM_001414954            4852 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus spastin (Spast), transcript variant 1, mRNA.
ACCESSION   NM_001414954 XM_008764487
VERSION     NM_001414954.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Yang,Y., Yang,J., Liang,Y., Zhang,G., Cai,Z., Zhang,Y., Lin,H. and
            Tan,M.
  TITLE     Rab3A interacts with spastin to regulate neurite outgrowth in
            hippocampal neurons
  JOURNAL   Biochem Biophys Res Commun 643, 77-87 (2023)
   PUBMED   36587525
  REMARK    GeneRIF: Rab3A interacts with spastin to regulate neurite outgrowth
            in hippocampal neurons.
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Tan,D., Zhang,H., Deng,J., Liu,J., Wen,J., Li,L., Wang,X., Pan,M.,
            Hu,X. and Guo,J.
  TITLE     RhoA-GTPase Modulates Neurite Outgrowth by Regulating the
            Expression of Spastin and p60-Katanin
  JOURNAL   Cells 9 (1), 230 (2020)
   PUBMED   31963385
  REMARK    GeneRIF: RhoA-GTPase Modulates Neurite Outgrowth by Regulating the
            Expression of Spastin and p60-Katanin.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4852)
  AUTHORS   Valenstein,M.L. and Roll-Mecak,A.
  TITLE     Graded Control of Microtubule Severing by Tubulin Glutamylation
  JOURNAL   Cell 164 (5), 911-921 (2016)
   PUBMED   26875866
REFERENCE   4  (bases 1 to 4852)
  AUTHORS   Vietri,M., Schink,K.O., Campsteijn,C., Wegner,C.S., Schultz,S.W.,
            Christ,L., Thoresen,S.B., Brech,A., Raiborg,C. and Stenmark,H.
  TITLE     Spastin and ESCRT-III coordinate mitotic spindle disassembly and
            nuclear envelope sealing
  JOURNAL   Nature 522 (7555), 231-235 (2015)
   PUBMED   26040712
REFERENCE   5  (bases 1 to 4852)
  AUTHORS   Goyal,U., Renvoise,B., Chang,J. and Blackstone,C.
  TITLE     Spastin-interacting protein NA14/SSNA1 functions in cytokinesis and
            axon development
  JOURNAL   PLoS One 9 (11), e112428 (2014)
   PUBMED   25390646
  REMARK    GeneRIF: NA14 may act as an adaptor protein regulating SPG4
            localization to centrosomes, temporally and spatially regulating
            the microtubule-severing activity of SPG4 that is particularly
            critical during the cell cycle and neuronal development.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4852)
  AUTHORS   Salinas,S., Carazo-Salas,R.E., Proukakis,C., Cooper,J.M.,
            Weston,A.E., Schiavo,G. and Warner,T.T.
  TITLE     Human spastin has multiple microtubule-related functions
  JOURNAL   J Neurochem 95 (5), 1411-1420 (2005)
   PUBMED   16219033
REFERENCE   7  (bases 1 to 4852)
  AUTHORS   Claudiani,P., Riano,E., Errico,A., Andolfi,G. and Rugarli,E.I.
  TITLE     Spastin subcellular localization is regulated through usage of
            different translation start sites and active export from the
            nucleus
  JOURNAL   Exp Cell Res 309 (2), 358-369 (2005)
   PUBMED   16026783
REFERENCE   8  (bases 1 to 4852)
  AUTHORS   Evans,K.J., Gomes,E.R., Reisenweber,S.M., Gundersen,G.G. and
            Lauring,B.P.
  TITLE     Linking axonal degeneration to microtubule remodeling by
            Spastin-mediated microtubule severing
  JOURNAL   J Cell Biol 168 (4), 599-606 (2005)
   PUBMED   15716377
REFERENCE   9  (bases 1 to 4852)
  AUTHORS   Charvin,D., Cifuentes-Diaz,C., Fonknechten,N., Joshi,V., Hazan,J.,
            Melki,J. and Betuing,S.
  TITLE     Mutations of SPG4 are responsible for a loss of function of
            spastin, an abundant neuronal protein localized in the nucleus
  JOURNAL   Hum Mol Genet 12 (1), 71-78 (2003)
   PUBMED   12490534
REFERENCE   10 (bases 1 to 4852)
  AUTHORS   Mead,S.H., Proukakis,C., Wood,N., Crosby,A.H., Plant,G.T. and
            Warner,T.T.
  TITLE     A large family with hereditary spastic paraparesis due to a frame
            shift mutation of the spastin (SPG4) gene: association with
            multiple sclerosis in two affected siblings and epilepsy in other
            affected family members
  JOURNAL   J Neurol Neurosurg Psychiatry 71 (6), 788-791 (2001)
   PUBMED   11723204
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            On Dec 16, 2022 this sequence version replaced XM_008764487.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR22582416.576399.1,
                                           SRR26643298.34314.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-680               JAXUCZ010000006.1  26857767-26858446   c
            681-764             JAXUCZ010000006.1  26846270-26846353   c
            765-848             JAXUCZ010000006.1  26842785-26842868   c
            849-944             JAXUCZ010000006.1  26839278-26839373   c
            945-1132            JAXUCZ010000006.1  26833468-26833655   c
            1133-1266           JAXUCZ010000006.1  26831899-26832032   c
            1267-1360           JAXUCZ010000006.1  26831572-26831665   c
            1361-1435           JAXUCZ010000006.1  26829575-26829649   c
            1436-1507           JAXUCZ010000006.1  26828774-26828845   c
            1508-1583           JAXUCZ010000006.1  26827203-26827278   c
            1584-1675           JAXUCZ010000006.1  26826719-26826810   c
            1676-1755           JAXUCZ010000006.1  26825919-26825998   c
            1756-1798           JAXUCZ010000006.1  26823413-26823455   c
            1799-1878           JAXUCZ010000006.1  26821375-26821454   c
            1879-1949           JAXUCZ010000006.1  26818363-26818433   c
            1950-1990           JAXUCZ010000006.1  26811323-26811363   c
            1991-4852           JAXUCZ010000006.1  26807220-26810081   c
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q13"
     gene            1..4852
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /note="spastin"
                     /db_xref="GeneID:362700"
                     /db_xref="RGD:1308494"
     exon            1..680
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     CDS             272..2113
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /EC_number="5.6.1.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     spastic paraplegia 4 (autosomal dominant; spastin)"
                     /codon_start=1
                     /product="spastin isoform 1"
                     /protein_id="NP_001401883.1"
                     /db_xref="GeneID:362700"
                     /db_xref="RGD:1308494"
                     /translation="MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAPGSPH
                     KRNLYYFSYPLVVGFALLRLLACHLGLLFVWLCQRFSRALMAAKRSSGTAPAPASPST
                     PAPGPGGEAESVRVFHKQAFEYISIALRIDEEEKGQKEQAVEWYKKGIEELEKGIAVI
                     VTGQGEQYERARRLQAKMMTNLVMAKDRLQLLEKLQPVLQFSKSQTDVYNESTNLTCR
                     NGHLQSESGAVPKKKDPLTHASNSLPRSKTVMKSGSTGLSGHHRAPSCSGLSMVSGAR
                     PGSGPAATTHKGTSKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGT
                     AVKFDDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAV
                     AAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREG
                     EHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNE
                     ETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQV
                     KNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV"
     exon            681..764
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            765..848
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            849..944
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1132
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1266
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1360
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1435
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1507
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1583
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1675
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1755
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1798
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1878
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..1949
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..1990
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..4852
                     /gene="Spast"
                     /gene_synonym="Spg4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctgagaggt gccagtgggc cagaggacag aggggaagga ggggtaggag ccaccggccg
       61 ctgcaggagg aggagacggg gttgtgctcc tggccagcgg aggagaaagg ggcggggccg
      121 gcgagcggcg cgtgcaggtg ccgggctccc gggttccgcg ggcgcgcgag cggctccgtg
      181 gcccccgccg ccgcagtggc cgccgccgcc gcttggtcgc cgtcggtctg cgggaagcgg
      241 gttatggcgg cggcggcagt gggagctgtg aatgagttct ccggccggac gacggaagaa
      301 gaaaggctca ggcggcgcga gccccgcgcc cgccaggcct ccgccccccg ccgcggtccc
      361 cgcccctgcc gccggcccgg cccctgcgcc cggctcgccg cataagcgga acctgtatta
      421 cttctcgtat ccgctggtcg tcggcttcgc cctgctgcgc ctgctggcct gccacctggg
      481 gctcctcttc gtgtggctct gccagcgctt ctcccgcgcc ctcatggccg ccaagaggag
      541 ctccgggacc gcgccggcgc ccgcctcgcc ctcgactcca gcccccggac cgggtggcga
      601 ggccgagagc gtccgcgtct tccacaagca ggcctttgag tacatctcca ttgccctgcg
      661 catcgacgag gaagagaaag gacagaagga acaagctgtg gaatggtata agaaaggcat
      721 cgaagaacta gagaaaggaa tcgcggttat agttacgggc caaggtgaac agtacgaaag
      781 agctagacgc cttcaagcca aaatgatgac gaatttggtt atggctaagg accgtttaca
      841 actcctagag aagctgcaac cagttttgca attttccaag tcacaaacgg acgtctataa
      901 tgagagcact aacctgacat gccgcaatgg acatctccag tcagaaagtg gagcagttcc
      961 gaagaagaaa gaccccttaa cacatgctag taattcattg cctcgatcaa aaactgtcat
     1021 gaaaagtgga tccacagggc tctccggtca ccacagggcg cctagttgca gtggtttatc
     1081 catggtttct ggagcaagac cgggatctgg tcctgcagct accacacata agggtacttc
     1141 aaaaccaaat agaaccaaca aaccttctac acccacaact gcagttcgga aaaagaaaga
     1201 cttgaaaaat tttaggaatg tggacagcaa tcttgctaac cttataatga atgaaattgt
     1261 tgacaatggg acagctgtta aatttgatga tatagctggt caggaactgg caaaacaagc
     1321 attgcaagag attgtcattc tcccttctct gcggcctgag ttgttcacag ggctcagagc
     1381 tcccgccaga ggcttgttac tcttcggtcc accaggaaac ggaaaaacca tgctggctaa
     1441 agcagtagct gcagaatcta atgcgacttt tttcaatata agtgctgcaa gtttaacttc
     1501 aaaatatgta ggagagggag agaaattggt gagagctctt tttgctgtgg ctcgagaact
     1561 tcaaccctct ataattttta tagatgaagt tgatagtctt ttgtgtgaga gaagagaagg
     1621 ggagcacgat gctagtagac gactaaaaac tgaattttta atagaatttg atggtgtgca
     1681 gtctgctgga gatgacagag tcctcgtaat gggtgcaacc aacaggcccc aagagctgga
     1741 tgaagctgtt ctcaggcgtt tcattaaacg ggtgtatgtg tcattaccca atgaggagac
     1801 acgactactt ctacttaaaa acctgttatg taaacaagga agtccactga cccaaaaaga
     1861 acttgctcaa ctggcgagaa tgactgatgg atactctgga agtgatctga ctgctttggc
     1921 aaaggatgca gcactgggtc ctatccgaga actgaaacca gagcaggtga agaacatgtc
     1981 tgccagtgag atgagaaata ttcgattatc tgacttcaca gaatccttaa aaaagatcaa
     2041 acgcagtgtg agtcctcaga ctttagaagc atacatacgc tggaacaagg actttggaga
     2101 caccactgtt taaaggaatg aatgcctttg taagcccaca ggacgctgta cttaacaaga
     2161 aacaagagct tcagctacag gaacctagac ttcgtttaca ggacttttta gagttttcat
     2221 ttttgtgcac caaacttgaa gaggaacaag aagacagatc taaataaaat atgcaatatg
     2281 aatggaactt ttgtttaagg ccttcttgtg tgatggtgat ggttttctca atgggcactg
     2341 ttagagcaca gcagaaatac attctgggtt attactgata ataatcatta tgtaaataat
     2401 aatttgtata ttgtgttcag atgaaagtat tccagagaca gtggatggta gaagacacaa
     2461 agagccctct gttttctgtc tcctgtgttt tcttgcaata gtctattttc tggcttcctt
     2521 tcctactgtt ccattagcac cctaagattg gagtaacaag cgctccaagc attttcctgc
     2581 tttcttacca actccagtgt gccaaatgga aatcttccca tctgcagaaa gagagctata
     2641 ctgaggcttt tcccacctag atctacaaac attaaacact taatatgttc tttttcatgt
     2701 tttgtttttc tgttgtattg ccttgctgcc aaccagttta gaaagcagtt tcctagccac
     2761 acaggaaacc tggcacagcc gaggtttgga agtctgagtc actgtctgtg tagcatgtat
     2821 atcagtcctc ggtgccctgc agtgttcttt tctaatatgt cagaagttca ctgtagactt
     2881 aatgtacccc atctgaaatt cctttctgtt taaattactc tgatatttag agagcaagtt
     2941 ttaacttaca gttgaggcac aggtttgaga gaagggacag atcaggaggt cagacatttc
     3001 tggtttattg ctatcacttc cttactgttt acagacaact aaatgccagt ggcattacca
     3061 ccttttacta attactgtcc cttccataaa actgaaaatc ctgaattttt ctacagggca
     3121 tatattttat aggtttcttg tgcttacttt gtaactaaaa agcgctagtc tgttgatcta
     3181 ccttttgttt ccataaaatg tatagtaact ctttgttgtt tgtataaata cggctggact
     3241 tttgttataa aaatgcatta taggaagtag aggctcatgt gaggcctttt cagaattgta
     3301 taaaggcaaa tagatatttg ccgctagttt actggttaaa agtttgttta cagaattttt
     3361 ctttggtgct taaatgatgc tatgtaaact ttcatgggta gaaagaataa ttttgtgata
     3421 gtaacactca acttttcatg taagaaacat gtttgagaga acacactaaa acacttgccc
     3481 tttcccccag ataaaatttt atgattaaga catacaacct caattttacc cttggtcttg
     3541 ttaaagattt aatacttcaa catagaagat gtattatttc ttagaatcat gcgctgtttt
     3601 tagctgtaat ttaaatcaca tcttttaatc atgacttttg acatccctgg atcataaaaa
     3661 ttaagtttcg atcaatagga aaacaaattt aaaccaaagc tgtgtataag ttttgttctg
     3721 gcagtctttt taaggtctca gatgttacac agttataaaa cactgatgat gtcaccccgt
     3781 gaagatggtg agaggacctc cagcagtcct aacccagagc atctgcaata aggctctaaa
     3841 actgcatttt atttcacgta ggaggtgact aataacattc ctactattta tgtattaaga
     3901 tggccttcag ctcagcgttc aaataggaga gcctttaaaa tcctagttca ttcatgattc
     3961 tttcaggaca ggtattagga ggtaacaatt taaatatcta aaagatgcaa actgaagtta
     4021 acctaaaatt ccgaatcaaa ggtgttcctg cgtgtctgtc tgtctgtcgg tgtagagaag
     4081 cacctttgtt catcaggttg gtggtttcag ctactgtaga aaacgttggc tgtgatgctg
     4141 ctgtatggcc caggtatgtt gtaggccatt ttgttattgt cttaagatag gagcctaagc
     4201 tggcctagaa ctcgatgggt agcctataaa caccttaaac tgattgtcat gcctgcattt
     4261 ctcacgcact gggcatgctg gtgtgcaccc acacccggag ggaggtttgc tggggttttc
     4321 taaattaaca ttagatttta attaattttc gatttgtgtc gagctctgtt tactgttcaa
     4381 gatgtttaaa taagaaggat tggtagttct ccatgttaac atgacagagt ccttgtaagc
     4441 tcggtgaact tactggtcac ctaactgagc caaaggaaag actcaatacg atctttccgg
     4501 atctgctagg gctataaagc ttttccgaga ctagacgccc attgaattta tttttgtctt
     4561 tttgtgttta acaggacaaa cagaatttta agttaaatat atgtggcatt ctttgtttat
     4621 actgtaaaat tgtttatata tcatacaaaa gctaattctt taatagaatg aactacttaa
     4681 cagcttgctt ttcgttttct taagtcagat tttcctttaa atgtcttgtc tcagttttag
     4741 tcttttactt ctgcaaagca aatgacttaa agagtacgta cagctacata aagtgggttt
     4801 ttatcgtaat taattttatt gtaaataaat gacaaacttt gttttgcctt aa
//
