LOCUS       NM_001106433            3734 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus spartin (Spart), mRNA.
ACCESSION   NM_001106433 XM_001059023 XM_215564
VERSION     NM_001106433.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Renvoise,B., Stadler,J., Singh,R., Bakowska,J.C. and Blackstone,C.
  TITLE     Spg20-/- mice reveal multimodal functions for Troyer syndrome
            protein spartin in lipid droplet maintenance, cytokinesis and BMP
            signaling
  JOURNAL   Hum Mol Genet 21 (16), 3604-3618 (2012)
   PUBMED   22619377
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Urbanczyk,A. and Enz,R.
  TITLE     Spartin recruits PKC-zeta via the PKC-zeta-interacting proteins
            ZIP1 and ZIP3 to lipid droplets
  JOURNAL   J Neurochem 118 (5), 737-748 (2011)
   PUBMED   21707618
REFERENCE   3  (bases 1 to 3734)
  AUTHORS   Joshi,D.C. and Bakowska,J.C.
  TITLE     SPG20 protein spartin associates with cardiolipin via its
            plant-related senescence domain and regulates mitochondrial Ca2+
            homeostasis
  JOURNAL   PLoS One 6 (4), e19290 (2011)
   PUBMED   21559443
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3734)
  AUTHORS   Renvoise,B., Parker,R.L., Yang,D., Bakowska,J.C., Hurley,J.H. and
            Blackstone,C.
  TITLE     SPG20 protein spartin is recruited to midbodies by ESCRT-III
            protein Ist1 and participates in cytokinesis
  JOURNAL   Mol Biol Cell 21 (19), 3293-3303 (2010)
   PUBMED   20719964
REFERENCE   5  (bases 1 to 3734)
  AUTHORS   Edwards,T.L., Clowes,V.E., Tsang,H.T., Connell,J.W.,
            Sanderson,C.M., Luzio,J.P. and Reid,E.
  TITLE     Endogenous spartin (SPG20) is recruited to endosomes and lipid
            droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5
  JOURNAL   Biochem J 423 (1), 31-39 (2009)
   PUBMED   19580544
  REMARK    Erratum:[Biochem J. 2009 Nov 1;423(3):442]
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Feb 11, 2021 this sequence version replaced NM_001106433.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643288.9432.1,
                                           SRR26643294.8773.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-89                JAXUCZ010000002.1  141442770-141442858
            90-892              JAXUCZ010000002.1  141447714-141448516
            893-1090            JAXUCZ010000002.1  141452064-141452261
            1091-1276           JAXUCZ010000002.1  141454949-141455134
            1277-1400           JAXUCZ010000002.1  141456517-141456640
            1401-1595           JAXUCZ010000002.1  141459016-141459210
            1596-1754           JAXUCZ010000002.1  141459811-141459969
            1755-1845           JAXUCZ010000002.1  141460087-141460177
            1846-3734           JAXUCZ010000002.1  141467500-141469388
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q26"
     gene            1..3734
                     /gene="Spart"
                     /gene_synonym="Spg20"
                     /note="spartin"
                     /db_xref="GeneID:295053"
                     /db_xref="RGD:1305900"
     exon            1..89
                     /gene="Spart"
                     /gene_synonym="Spg20"
                     /inference="alignment:Splign:2.1.0"
     exon            90..892
                     /gene="Spart"
                     /gene_synonym="Spg20"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..2116
                     /gene="Spart"
                     /gene_synonym="Spg20"
                     /note="spastic paraplegia 20, spartin (Troyer syndrome)
                     homolog; spastic paraplegia 20 (Troyer syndrome) homolog"
                     /codon_start=1
                     /product="spartin"
                     /protein_id="NP_001099903.3"
                     /db_xref="GeneID:295053"
                     /db_xref="RGD:1305900"
                     /translation="MERGAENGEPPEMKIIKEAYEKAFMFVNKGLNTDELGQKEEAKT
                     YYKQGIGHLLRGISIAAAEPGHTGPAWEAARQMQQKMKETLQNVRTRLEILEKGLATS
                     LRNDLQDVPKLYPEFPPKGTCKKSPEAESAPQHAEVDGSASAAGAGPSAAPSALPLPS
                     PGCPAEAPPAYTPQAAEGHYTVSYGTDSGEFSSVGEDFYRNRSQPPPLETLGLDADEL
                     ILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLV
                     PDRSPVLKCTVGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQA
                     NWNREEEEEEEEEEDEFQIPGRVKELPKEASGTDVRQSSSSGSSIDQGSKDARHKGKR
                     GKKTKDSSSEEVNLRQIVPCEPSSEEKSKDLPEWSEKVAHNILSGASWVSWGLVKGAE
                     FTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQFLVDGVCTV
                     ANCVGKELAPHVKKHGSKLVPESLKRDKDGKSTLDGAMVVAASSVQGFSTVWQGLECA
                     AKCIVNNVSAETVQTVRYKYGHNAGEATHNAVDSAINVGVTAYNIDNIGIKAMVKKTA
                     KQTGHTLLEDYQIIESPQRESQGGATSTEGTRDVERKMEEEKKGAKKKDK"
     exon            893..1090
                     /gene="Spart"
                     /gene_synonym="Spg20"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1276
                     /gene="Spart"
                     /gene_synonym="Spg20"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1400
                     /gene="Spart"
                     /gene_synonym="Spg20"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1595
                     /gene="Spart"
                     /gene_synonym="Spg20"
                     /inference="alignment:Splign:2.1.0"
     exon            1596..1754
                     /gene="Spart"
                     /gene_synonym="Spg20"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1845
                     /gene="Spart"
                     /gene_synonym="Spg20"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..3734
                     /gene="Spart"
                     /gene_synonym="Spg20"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctgaggcc ggagggtgtt tcggacctgc agctgtgcgc cttccgtggg ttctcaggct
       61 cggagcggac ctccgtgcgc ctttggaaga gatggagaga ggagcagaaa atggagagcc
      121 tcctgaaatg aagatcatca aggaggcgta cgagaaggcc tttatgtttg tcaataaagg
      181 actgaacaca gacgaattag ggcagaagga agaggcaaag acctattaca agcagggaat
      241 agggcacctg cttaggggca tcagcatcgc ggcggcagag ccagggcaca caggccctgc
      301 gtgggaggcg gccagacaga tgcaacagaa aatgaaggaa acgcttcaga acgtccgcac
      361 caggctagag atcctggaga agggcctcgc cacttctcta cggaatgacc ttcaggacgt
      421 gcccaagtta tacccagagt tccctcctaa aggcacgtgc aagaagtccc cagaggccga
      481 gtcagctccc cagcatgctg aagtggatgg aagcgcctca gctgcgggtg ctgggccaag
      541 tgccgcgcct tccgctctgc ccttaccatc gcccggctgc cctgccgaag cgccaccggc
      601 ctacacccca caggctgccg aaggccacta cactgtttcc tacgggacag actctgggga
      661 gttttcatcg gtgggagaag acttctatag gaaccgttcc cagccgcccc ctcttgagac
      721 cttggggctc gatgcagatg agctgatctt gatcccaaat ggagtgcaga tcttttttgt
      781 aaaccctgcg ggagaggtca gtgctccctc ataccctggc tacctgcgga tcgtgaggtt
      841 cttggataat tctctggata cagttctgaa ccgccctcct ggatttctcc aggtgtgtga
      901 ctggttatac cctctagttc ctgataggtc accagttctc aagtgcaccg tgggagcata
      961 catgtttcca gacaccatgt tacaagctgc cgggtgcttt gtgggggttg ttctctcatc
     1021 tgagctccca gaagatgaca gagagctgtt tgaagatctg ttgagacaga tgtctgacct
     1081 tcggctccag gccaactgga atagagaaga agaagaagag gaagaagaag aagaagatga
     1141 attccaaatc cctgggagag taaaggaact accaaaggaa gcgtctggca ctgatgtcag
     1201 gcagtcaagt tcttcaggtt cttcaataga ccaaggcagc aaggatgcac gccataaagg
     1261 aaagcgtggg aaaaagacta aagattcttc aagtgaagaa gttaatctga gacagatcgt
     1321 gccttgtgag ccgagttcag aagaaaaatc gaaagacctg cctgaatgga gcgagaaagt
     1381 ggcccacaac attctgtcag gtgcttcctg ggtgagctgg ggtttggtca aaggtgctga
     1441 atttactggc aaagcaattc agaaaggtgc ttctaaactc agagagcgca ttcagccaga
     1501 ggaaaagcca gtggaagtga gtccagctgt taccaggggc ctctacatag cgaagcaggc
     1561 aactggagga gccgcaaaag tcagtcagtt cctggttgat ggcgtctgca cagtagcgaa
     1621 ttgtgttggc aaagaactcg ctccacatgt caagaagcat ggaagcaaac tcgttccaga
     1681 atctctgaag agagacaaag atgggaagtc taccctggat ggcgccatgg ttgtggcagc
     1741 aagtagtgtt caaggattct caactgtctg gcagggactg gaatgtgcag ctaaatgcat
     1801 cgttaacaac gtgtctgcag aaactgtaca gactgtcaga tacaagtatg ggcacaacgc
     1861 aggagaagct acacataatg cggtggactc ggctatcaat gttggggtca ctgcctacaa
     1921 tattgacaac atcggtatca aagccatggt gaagaaaact gccaagcaga cagggcacac
     1981 tctccttgag gactaccaga tcattgagag ccctcagagg gaaagtcagg gaggagcaac
     2041 gagcacagaa gggacaagag atgtggagag gaagatggag gaagagaaga aaggggcaaa
     2101 aaagaaagat aaataacagg tgttctggga gtgacccgca ccaaagcctt accagatgga
     2161 ggggtgtgga gaggatgtgg attctccaca gaagaacagc atcttctgtc agttcattcc
     2221 taccaagtga ctgttggaag tttggggcat gtatccactt acaaggaaaa taatcagtat
     2281 tcttacatct ggcttctaga catatgcagt ttgtagtctc agtaagttta ttttccctta
     2341 aatgtggcta aagtattttt gcagttaaat aaagcttgta atatatgtga tttaaaccta
     2401 atgtccatct atttttctat gtgctagcct tcagaaggca cagtaggaaa ctgcacatga
     2461 tctacagccc cctgagctct ggtcttacca ggaaacgact gaatgtcgat gcctttcttt
     2521 tgcactaatt gtgggtcttt tgagatgccc agcagcatat aagctaaaca aaactgtgat
     2581 cttaaagttt gttctcagat gatgttctag aaaattttct caaatgggga agagcttggc
     2641 catgaaagtg ccttgaagct ctgtaatgtt tcacttactt gctagctctc ctgcctccta
     2701 ggaaaccaaa atggccttgt agtttaaact gaagaaaatg catctttaag tctttagggt
     2761 tgaggctagg cactattttg atagaatgat ttctattaat tcttcgggaa ttttgtacaa
     2821 atataaatgt gttttgattg tatctcccta caacccccag ctcccccagg tccacacctt
     2881 acccccctcc cagcttcact ctcttttgtg tgtttgtttt aaataatcca gtctgtgctg
     2941 cccatatgat catgggtgtg gggccatcca ctggaggttg gttcatctgc cacggacctt
     3001 gttcctcaga agccaccaac tgtcagtagc tcctcagctg ggctggggct tgtgagaccc
     3061 tgcccactgc aagctggagt gctggctggt ttgagcctgt ggaggtggcc tcagctgcta
     3121 tgagtgtaag ggtcctattg tacccagaag atgtcagttt atccatgcct cctggacttc
     3181 tgactcttac agtctctcac tcctcttctt ccatgttccc tgagctttat gggaggggag
     3241 gagtgtggta tagactaggg aattttgaaa tatgattagt attctcttaa aatttgaatt
     3301 gattgttaat ggcattaaat cactttttga tatctgcttc tgtaaatttt tcctaaattt
     3361 ctggaaagta aaacacaaac attgcattta ctaagtgtaa tgtatctgtc agtcattaga
     3421 atttatacta aaagccagga agtgatgaga gaatcctgtc tttaccacag taaagaacat
     3481 ggtacccttt ggtgggtagt ccccacttct gcagaacaca ggatggacat ttaccagttt
     3541 tcttttaagt tacaaatata tacttaaata ctcatggcat tttatttctg ttaactttaa
     3601 aaggctgata ctggttttta aggtttttgc ctgttgtagt catttctgaa cctgtagttg
     3661 gctagcatat ggatttcatt tggaattggt tagtgaatgt gctttgcgaa taaaatggtt
     3721 tctgaattgc tggg
//
