LOCUS       NM_012656               2029 bp    mRNA    linear   ROD 09-APR-2024
DEFINITION  Rattus norvegicus secreted protein acidic and cysteine rich
            (Sparc), mRNA.
ACCESSION   NM_012656
VERSION     NM_012656.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Jiang,A., Xu,P., Yang,Z., Zhao,Z., Tan,Q., Li,W., Song,C., Dai,H.
            and Leng,H.
  TITLE     Increased Sparc release from subchondral osteoblasts promotes
            articular chondrocyte degeneration under estrogen withdrawal
  JOURNAL   Osteoarthritis Cartilage 31 (1), 26-38 (2023)
   PUBMED   36241137
  REMARK    GeneRIF: Increased Sparc release from subchondral osteoblasts
            promotes articular chondrocyte degeneration under estrogen
            withdrawal.
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Zhu,Y.S., Zhang,J.N., Mo,T.T., Jiang,C., Ma,R.C. and Chen,L.
  TITLE     Discoidin domain receptor 2 activation of p38 mitogen-activated
            protein kinase as an important pathway for osteonectin-regulating
            osteoblast mineralization
  JOURNAL   J Orthop Surg Res 16 (1), 711 (2021)
   PUBMED   34876214
  REMARK    GeneRIF: Discoidin domain receptor 2 activation of p38
            mitogen-activated protein kinase as an important pathway for
            osteonectin-regulating osteoblast mineralization.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2029)
  AUTHORS   Kim,J., Yang,Y.L., Jeong,Y. and Jang,Y.S.
  TITLE     Middle East Respiratory Syndrome-Coronavirus Infection into
            Established hDPP4-Transgenic Mice Accelerates Lung Damage Via
            Activation of the Pro-Inflammatory Response and Pulmonary Fibrosis
  JOURNAL   J Microbiol Biotechnol 30 (3), 427-438 (2020)
   PUBMED   31838832
REFERENCE   4  (bases 1 to 2029)
  AUTHORS   Zhou,S., Lei,D., Bu,F., Han,H., Zhao,S. and Wang,Y.
  TITLE     MicroRNA-29b-3p Targets SPARC Gene to Protect Cardiocytes against
            Autophagy and Apoptosis in Hypoxic-Induced H9c2 Cells
  JOURNAL   J Cardiovasc Transl Res 12 (4), 358-365 (2019)
   PUBMED   30560317
  REMARK    GeneRIF: miR-29b-3p plays an important role in the progression of
            heart failure through targeting SPARC and regulating TGFbeta1/Smad3
            pathway.
REFERENCE   5  (bases 1 to 2029)
  AUTHORS   Levin,G., Coelho,T.M., Nobrega,N.G., Trombetta-Lima,M.,
            Sogayar,M.C. and Carreira,A.C.O.
  TITLE     Spatio-temporal expression profile of matrix metalloproteinase
            (Mmp) modulators Reck and Sparc during the rat ovarian dynamics
  JOURNAL   Reprod Biol Endocrinol 16 (1), 116 (2018)
   PUBMED   30424792
  REMARK    GeneRIF: Data suggest that reversion-inducing-cysteine-rich protein
            with kazal motifs (Reck) and SPARC Glycoprotein (Sparc) seem to
            play a role during ovarian dynamics: Reck as a possible inhibitor
            and Sparc as an inducer of matrix metalloproteinases (Mmps).
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2029)
  AUTHORS   Sauk,J.J., Smith,T., Silbergeld,E.K., Fowler,B.A. and Somerman,M.J.
  TITLE     Lead inhibits secretion of osteonectin/SPARC without significantly
            altering collagen or Hsp47 production in osteoblast-like ROS 17/2.8
            cells
  JOURNAL   Toxicol Appl Pharmacol 116 (2), 240-247 (1992)
   PUBMED   1412468
REFERENCE   7  (bases 1 to 2029)
  AUTHORS   Breton-Gorius,J., Clezardin,P., Guichard,J., Debili,N., Malaval,L.,
            Vainchenker,W., Cramer,E.M. and Delmas,P.D.
  TITLE     Localization of platelet osteonectin at the internal face of the
            alpha-granule membranes in platelets and megakaryocytes
  JOURNAL   Blood 79 (4), 936-941 (1992)
   PUBMED   1737102
REFERENCE   8  (bases 1 to 2029)
  AUTHORS   Cheng,C.Y.
  TITLE     Purification of a calcium binding protein (rat SPARC) from primary
            Sertoli cell-enriched culture medium
  JOURNAL   Biochem Biophys Res Commun 167 (3), 1393-1399 (1990)
   PUBMED   2322281
REFERENCE   9  (bases 1 to 2029)
  AUTHORS   Wewer,U.M., Albrechtsen,R., Fisher,L.W., Young,M.F. and
            Termine,J.D.
  TITLE     Osteonectin/SPARC/BM-40 in human decidua and carcinoma, tissues
            characterized by de novo formation of basement membrane
  JOURNAL   Am J Pathol 132 (2), 345-355 (1988)
   PUBMED   3400777
REFERENCE   10 (bases 1 to 2029)
  AUTHORS   Engel,J., Taylor,W., Paulsson,M., Sage,H. and Hogan,B.
  TITLE     Calcium binding domains and calcium-induced conformational
            transition of SPARC/BM-40/osteonectin, an extracellular
            glycoprotein expressed in mineralized and nonmineralized tissues
  JOURNAL   Biochemistry 26 (22), 6958-6965 (1987)
   PUBMED   3427055
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Nov 26, 2020 this sequence version replaced NM_012656.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y13714.1, BC061777.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840107, SAMN12840111
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                JAXUCZ010000010.1  40038779-40038816   c
            39-108              JAXUCZ010000010.1  40029281-40029350   c
            109-165             JAXUCZ010000010.1  40026525-40026581   c
            166-253             JAXUCZ010000010.1  40025806-40025893   c
            254-375             JAXUCZ010000010.1  40024459-40024580   c
            376-496             JAXUCZ010000010.1  40023896-40024016   c
            497-630             JAXUCZ010000010.1  40021825-40021958   c
            631-779             JAXUCZ010000010.1  40021220-40021368   c
            780-928             JAXUCZ010000010.1  40018767-40018915   c
            929-2029            JAXUCZ010000010.1  40017065-40018165   c
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q22"
     gene            1..2029
                     /gene="Sparc"
                     /note="secreted protein acidic and cysteine rich"
                     /db_xref="GeneID:24791"
                     /db_xref="RGD:3742"
     exon            1..38
                     /gene="Sparc"
                     /inference="alignment:Splign:2.1.0"
     exon            39..108
                     /gene="Sparc"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..957
                     /gene="Sparc"
                     /note="osteonectin; ON; BM-40; basement-membrane protein
                     40; secreted protein acidic and rich in cysteine; Secreted
                     acidic cystein-rich glycoprotein (osteonectin); secreted
                     acidic cysteine rich glycoprotein; secreted protein,
                     acidic, cysteine-rich (osteonectin)"
                     /codon_start=1
                     /product="SPARC precursor"
                     /protein_id="NP_036788.1"
                     /db_xref="GeneID:24791"
                     /db_xref="RGD:3742"
                     /translation="MRAWIFFLLCLAGRALAAPQTEAAEEMVAEETVVEETGLPVGAN
                     PVQVEMGEFEEGAEETVEEVVAENPCQNHHCKHGKVCELDESNTPMCVCQDPTSCPAP
                     IGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIAPCLDSELTE
                     FPLRMRDWLKNVLVTLYERDEGNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDF
                     EKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDND
                     KYIALEEWAGCFGIKEQDINKDLVI"
     exon            109..165
                     /gene="Sparc"
                     /inference="alignment:Splign:2.1.0"
     exon            166..253
                     /gene="Sparc"
                     /inference="alignment:Splign:2.1.0"
     exon            254..375
                     /gene="Sparc"
                     /inference="alignment:Splign:2.1.0"
     exon            376..496
                     /gene="Sparc"
                     /inference="alignment:Splign:2.1.0"
     exon            497..630
                     /gene="Sparc"
                     /inference="alignment:Splign:2.1.0"
     exon            631..779
                     /gene="Sparc"
                     /inference="alignment:Splign:2.1.0"
     exon            780..928
                     /gene="Sparc"
                     /inference="alignment:Splign:2.1.0"
     exon            929..2029
                     /gene="Sparc"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tctgctgcct gcccaactgc ctgcctgcct gtgccgagag ttcccagcac catgagggcc
       61 tggatcttct ttctcctttg cctggccggg agggccctgg cagcccctca gacggaagct
      121 gcagaagaga tggtggcgga ggaaaccgtg gtggaggaga cagggttacc tgtgggtgcc
      181 aacccagtcc aggtggaaat gggagagttt gaagaaggtg cagaggaaac tgtcgaggag
      241 gtggtggctg aaaacccctg ccagaaccat cactgcaaac atggcaaggt gtgtgagctg
      301 gacgagagca acacccccat gtgtgtgtgc caggacccca ccagctgccc agctcccatt
      361 ggcgagtttg aaaaggtgtg cagcaatgac aacaagacct tcgactcttc ctgccacttc
      421 tttgcgacca agtgcaccct ggagggcacc aagaagggcc acaagctcca cctggactac
      481 atcggaccat gcaaatacat tgccccctgc ctggattctg agctgaccga attccctctg
      541 cgcatgcgtg actggctcaa aaacgtcctg gtcaccttgt acgagagaga tgagggcaac
      601 aacctcctca ctgagaagca gaaactgcgt gtgaagaaga tccacgagaa cgagaagcgc
      661 ctggaggctg gagaccaccc tgtggagctg ctggcccgag actttgagaa gaactacaac
      721 atgtacatct tccctgtcca ctggcagttt ggccagctgg atcagcaccc gattgatggg
      781 tacctgtccc acacggagct ggccccactg cgcgctcccc tcattcccat ggaacattgc
      841 accactcgct tctttgagac ctgtgaccta gacaatgaca agtacattgc cctggaggaa
      901 tgggccggct gcttcggcat caaggagcag gacatcaaca aggatctggt gatctaagtt
      961 caagcctcct gcagcagtcc tggactctct ccccctgatg tccccaccca cttccactac
     1021 ccccttgttt aaaatgtttg gatggttggc tgttctgcct ggggataagg tgctaacata
     1081 gatttaactg aatacattaa cggtgctaaa aaaaaaacaa aaaacaaaaa aaacagaaag
     1141 aaagaaacca gatcccaagt cacagcattt tcccacgtaa ctcgactctg aggccatagc
     1201 ctatccacag cctcctcgtc ccctgcaccg cccagtgtct cactggctgt gttggaaacg
     1261 ggaattgcat aagcttgcct tcctcaagca agaaatatct ctagctttca tttccatttt
     1321 gactcttaac actcacccag actctgtgct tatttcattt gggggggggg atgtgggctt
     1381 cctggggtct tcccctggta gtttggaggt aggcagaggg aagttacaga cacagataca
     1441 aaacttgggc aaggacgctg tgaggccagt cagaaccaga tggcaagtct tggtagccta
     1501 ggtcaacgac tgacagaata atccagagct ctgatgcaca aaacagactc ccagcagccc
     1561 gggaccttgc tgtctcctcc actcttcagg cagtttcttt ccatgtttgg ctgttggttt
     1621 taattttggt gagccaaggg gaggcatggg cagaccaata cctcactagg gattctctta
     1681 ctcaactgct ataggacttt caggctcttg ctgggagctc taggcactgg gctacaggaa
     1741 agtgagactc aagaggaaga cagagaaggt tgtaacgtag agagagtgag tcataaagtt
     1801 tcaagcatgc ccccccccac ctctccccac cctttgccac ttgaaactta ctaatcaaga
     1861 gaaacttcca agccaacgga aggaatggtc ggatctcaca ggctgagaat ttgttcccct
     1921 ccaagcattt catgaaaaag ctgcttctca ttaaccatgc gaactctcac agtgatgtga
     1981 agagcttgac agatctttca aaataaaaag taatgactta gaaatggcc
//
