LOCUS       NM_053967               2112 bp    mRNA    linear   ROD 04-DEC-2024
DEFINITION  Rattus norvegicus sperm adhesion molecule 1 (Spam1), mRNA.
ACCESSION   NM_053967
VERSION     NM_053967.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Miranda,P.V., Allaire,A., Sosnik,J. and Visconti,P.E.
  TITLE     Localization of low-density detergent-resistant membrane proteins
            in intact and acrosome-reacted mouse sperm
  JOURNAL   Biol Reprod 80 (5), 897-904 (2009)
   PUBMED   19144954
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Reitinger,S., Laschober,G.T., Fehrer,C., Greiderer,B. and
            Lepperdinger,G.
  TITLE     Mouse testicular hyaluronidase-like proteins SPAM1 and HYAL5 but
            not HYALP1 degrade hyaluronan
  JOURNAL   Biochem J 401 (1), 79-85 (2007)
   PUBMED   16925524
REFERENCE   3  (bases 1 to 2112)
  AUTHORS   Kim,E., Baba,D., Kimura,M., Yamashita,M., Kashiwabara,S. and
            Baba,T.
  TITLE     Identification of a hyaluronidase, Hyal5, involved in penetration
            of mouse sperm through cumulus mass
  JOURNAL   Proc Natl Acad Sci U S A 102 (50), 18028-18033 (2005)
   PUBMED   16330764
REFERENCE   4  (bases 1 to 2112)
  AUTHORS   Zhang,H., Jones,R. and Martin-DeLeon,P.A.
  TITLE     Expression and secretion of rat SPAM1(2B1 or PH-20) in the
            epididymis: role of testicular lumicrine factors
  JOURNAL   Matrix Biol 22 (8), 653-661 (2004)
   PUBMED   15062858
  REMARK    GeneRIF: Results describe the factors regulating the expression of
            epididymal sperm adhesion molecule SPAM1/2B1.
REFERENCE   5  (bases 1 to 2112)
  AUTHORS   Baba,D., Kashiwabara,S., Honda,A., Yamagata,K., Wu,Q., Ikawa,M.,
            Okabe,M. and Baba,T.
  TITLE     Mouse sperm lacking cell surface hyaluronidase PH-20 can pass
            through the layer of cumulus cells and fertilize the egg
  JOURNAL   J Biol Chem 277 (33), 30310-30314 (2002)
   PUBMED   12065596
REFERENCE   6  (bases 1 to 2112)
  AUTHORS   Hou,S.T., Ma,A., Jones,R. and Hall,L.
  TITLE     Molecular cloning and characterization of rat sperm surface antigen
            2B1, a glycoprotein implicated in sperm-zona binding
  JOURNAL   Mol Reprod Dev 45 (2), 193-203 (1996)
   PUBMED   8914077
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from X89999.1.
            
            On Aug 30, 2012 this sequence version replaced NM_053967.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X89999.1, BC081748.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2112              X89999.1           5-2116
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q22"
     gene            1..2112
                     /gene="Spam1"
                     /gene_synonym="Spam"
                     /note="sperm adhesion molecule 1"
                     /db_xref="GeneID:117037"
                     /db_xref="RGD:620547"
     exon            1..135
                     /gene="Spam1"
                     /gene_synonym="Spam"
                     /inference="alignment:Splign:2.1.0"
     exon            136..1277
                     /gene="Spam1"
                     /gene_synonym="Spam"
                     /inference="alignment:Splign:2.1.0"
     CDS             324..1862
                     /gene="Spam1"
                     /gene_synonym="Spam"
                     /EC_number="3.2.1.35"
                     /note="hyaluronidase PH-20; sperm surface protein PH-20;
                     hyaluronoglucosaminidase PH-20; hyal-PH20; sperm surface
                     antigen 2B1; sperm adhesion molecule 1 (PH-20
                     hyaluronidase, zona pellucida binding)"
                     /codon_start=1
                     /product="hyaluronidase PH-20 precursor"
                     /protein_id="NP_446419.1"
                     /db_xref="GeneID:117037"
                     /db_xref="RGD:620547"
                     /translation="MGELQFKWLFWRSFAESGGTFQTVLIFLFIPYSLTVDYRATPVL
                     SDTTFVWVWNVPTEACVENVTEPIDLSFFSLIGSPRKTAIGQPVTLFYVDRLGNYPHI
                     DAQQTEHHGGIPQKGDLTTHLVKAKEDVERYIPTDKLGLAIIDWEEWRPTWMRNWTPK
                     DIYRNKSIELVQAADPAINITEATVRAKAQFEGAAKEFMEGTLKLGKHIRPKHLWGFY
                     LFPDCYNNKFQVDNYDGQCPDVEKKRNDDLDWLWKESTGLYPSVYLKKDLKSSRKATL
                     YVRYRVLESIRVSKVSDESNPVPIFVYIRLVFTDHVSEYLLEDDLVNTIGEIVAQGTS
                     GIIIWDAMSLAQRSAGCPILRQYMKTTLNPYIVNVTLAAKMCSQTLCKEKGMCSRKTE
                     SSDAYLHLDPSSFSINVTEAGKYEVLGKPEVKDLEYFSEHFKCSCFSKMTCEETSDMR
                     SIQDVNVCMGDNVCIKATLGPNSAFHLLPGKGLLLMTTLAHILHHLPHDIFVFPWKML
                     VSTP"
     exon            1278..1367
                     /gene="Spam1"
                     /gene_synonym="Spam"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..2102
                     /gene="Spam1"
                     /gene_synonym="Spam"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtcctctgt agaccttgcc ttggtctagg tcgcttgagt agatttaggt tgaccatgac
       61 tcacatgaat taagattttc ttttgttatg atggagatgc agatggtagg caagtgattc
      121 taagtttcca gcaagttctg gatgttttaa cttgccgcaa gatattcctg aaacgtaaca
      181 caagaagaag aatcttctgt gtaaattagt cacatacttt attctcccac atgtcacaat
      241 ccttctaatc tattgcatca aattctggac agactgaagt atataagaag aaataagtgc
      301 ttacatagta attattctcc gtaatgggag agttgcaatt taagtggctc ttttggagga
      361 gctttgctga gtctgggggc acattccaaa cagtgttaat ctttcttttc attccatatt
      421 ccttgactgt ggattatagg gcaacaccag ttctgtcaga tacaactttc gtttgggtct
      481 ggaatgttcc aactgaagct tgtgtagaaa acgttactga accaatagat ctgagcttct
      541 tctctttaat tggaagcccc cggaaaactg ccatagggca gcctgtcaca ttattttatg
      601 ttgatcgact tggtaactat cctcacatag atgcacagca aacagaacat catggaggaa
      661 tacctcagaa gggggatttg acaactcatt tggtgaaagc taaggaagac gtagagcgtt
      721 acataccaac agacaaactg ggtttagcta tcattgactg ggaagaatgg aggcctacct
      781 ggatgagaaa ctggacacct aaggatattt acaggaataa gtctattgaa ttggtccagg
      841 cagctgatcc agcaattaat atcacagaag ctactgtgag agccaaagca caatttgaag
      901 gggcagctaa agagtttatg gaagggactt taaaattggg gaaacatatt cgaccaaagc
      961 acttatgggg tttttatcta tttcctgatt gttataacaa taaatttcaa gtcgataact
     1021 atgatggcca atgccctgat gtggaaaaga aacgaaacga tgatcttgac tggctatgga
     1081 aagaaagcac gggcctttac ccatctgtct atttgaagaa agatttgaag tcgagtcgaa
     1141 aagcaaccct ttatgtccgc taccgagttt tagaatctat cagagtgtcc aaagtttcag
     1201 atgaatcgaa tccagttcca atttttgtct atatccgtct tgttttcact gatcatgtct
     1261 ctgaatacct tctagaggat gatcttgtga atacaattgg tgaaattgtt gctcagggta
     1321 cctctggaat tataatatgg gatgctatgt ctttagcaca acgttcggca ggttgcccaa
     1381 tcctacgtca atacatgaag acaaccctga atccatacat agtcaatgtc accctagcag
     1441 ccaaaatgtg cagtcaaaca ctttgtaagg agaaaggcat gtgttcgaga aaaacagaaa
     1501 gttcagatgc atatcttcac ttggatccaa gtagtttttc tattaatgta acagaagctg
     1561 gaaagtatga agttcttggc aagcccgagg ttaaagactt ggaatacttt tctgaacatt
     1621 ttaaatgcag ctgttttagc aaaatgacat gtgaggagac atctgatatg cgaagtatac
     1681 aagatgtgaa tgtgtgcatg ggtgacaatg tttgtataaa agccacgcta ggacccaact
     1741 cagcattcca cctcctacct ggcaaaggcc tcctccttat gacaacactt gctcacatct
     1801 tgcaccatct accacatgat atttttgttt ttccgtggaa gatgctagtc agtactcctt
     1861 agttttctct atccagagca tttgctatat tatttttttg caggcctcgg taatttggga
     1921 ttatgaatgt gattctattt tgcaaaagca attcaatttt aataatcaag attctatttt
     1981 tgagtttcaa agacaaatta tatactcttc caccaaagac tgattgcaag caaggctact
     2041 gaacttagag attatttttt gtttaaagag aatgaaaact gaataaaata actaactata
     2101 aaaaaaaaaa aa
//
