LOCUS       NM_001044224            3881 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus sperm associated antigen 5 (Spag5), mRNA.
ACCESSION   NM_001044224 XM_001080820 XM_340848
VERSION     NM_001044224.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Li Y, Diao Y, Wang Z, Wang S, Peng J and Kong B.
  TITLE     The splicing factor SF3B4 drives proliferation and invasion in
            cervical cancer by regulating SPAG5
  JOURNAL   Cell Death Discov 8 (1), 326 (2022)
   PUBMED   35853859
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Chu X, Chen X, Wan Q, Zheng Z and Du Q.
  TITLE     Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulates
            Astrin at the Mitotic Spindle
  JOURNAL   J Biol Chem 291 (38), 20055-20067 (2016)
   PUBMED   27462074
REFERENCE   3  (bases 1 to 3881)
  AUTHORS   Kodani A, Yu TW, Johnson JR, Jayaraman D, Johnson TL, Al-Gazali L,
            Sztriha L, Partlow JN, Kim H, Krup AL, Dammermann A, Krogan NJ,
            Walsh CA and Reiter JF.
  TITLE     Centriolar satellites assemble centrosomal microcephaly proteins to
            recruit CDK2 and promote centriole duplication
  JOURNAL   Elife 4, e07519 (2015)
   PUBMED   26297806
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3881)
  AUTHORS   Katayama K, Yasuda H, Tochigi Y and Suzuki H.
  TITLE     The microtubule-associated protein astrin transgenesis rescues
            spermatogenesis and renal function in hypogonadic (hgn/hgn) rats
  JOURNAL   Andrology 1 (2), 301-307 (2013)
   PUBMED   23413142
  REMARK    GeneRIF: astrin (spag5) is necessary for the establishment of
            normal size (cell number) and function of the testis and kidney in
            rats.
REFERENCE   5  (bases 1 to 3881)
  AUTHORS   Asami M, Pilz GA, Ninkovic J, Godinho L, Schroeder T, Huttner WB
            and Gotz M.
  TITLE     The role of Pax6 in regulating the orientation and mode of cell
            division of progenitors in the mouse cerebral cortex
  JOURNAL   Development 138 (23), 5067-5078 (2011)
   PUBMED   22031545
REFERENCE   6  (bases 1 to 3881)
  AUTHORS   Dunsch AK, Linnane E, Barr FA and Gruneberg U.
  TITLE     The astrin-kinastrin/SKAP complex localizes to microtubule plus
            ends and facilitates chromosome alignment
  JOURNAL   J Cell Biol 192 (6), 959-968 (2011)
   PUBMED   21402792
REFERENCE   7  (bases 1 to 3881)
  AUTHORS   Yagi M, Takenaka M, Suzuki K and Suzuki H.
  TITLE     Reduced mitotic activity and increased apoptosis of fetal sertoli
            cells in rat hypogonadic (hgn/hgn) testes
  JOURNAL   J Reprod Dev 53 (3), 581-589 (2007)
   PUBMED   17310077
  REMARK    GeneRIF: Spag5 mutation may cause a reduction in mitotic activity
            and an increase in apoptosis of fetal Sertoli cells in hgn/hgn
            testes.
REFERENCE   8  (bases 1 to 3881)
  AUTHORS   Fitzgerald CJ, Oko RJ and van der Hoorn FA.
  TITLE     Rat Spag5 associates in somatic cells with endoplasmic reticulum
            and microtubules but in spermatozoa with outer dense fibers
  JOURNAL   Mol Reprod Dev 73 (1), 92-100 (2006)
   PUBMED   16211599
  REMARK    GeneRIF: Different localization of Spag5 in somatic versus male
            germ cells suggests the possibility of different function.
REFERENCE   9  (bases 1 to 3881)
  AUTHORS   Chang MS, Chen CY, Huang CJ, Fan CC, Chu JM and Yang YC.
  TITLE     Expression and promoter analysis of mouse mastrin gene
  JOURNAL   Biochem Biophys Res Commun 307 (3), 491-497 (2003)
   PUBMED   12893248
REFERENCE   10 (bases 1 to 3881)
  AUTHORS   Shao X, Xue J and van der Hoorn FA.
  TITLE     Testicular protein Spag5 has similarity to mitotic spindle protein
            Deepest and binds outer dense fiber protein Odf1
  JOURNAL   Mol Reprod Dev 59 (4), 410-416 (2001)
   PUBMED   11468777
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB231661.1.
            
            On or before Aug 24, 2006 this sequence version replaced
            XM_340848.3, XM_001080820.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB231661.1, BC166408.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="HGN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q25"
     gene            1..3881
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /note="sperm associated antigen 5"
                     /db_xref="GeneID:252918"
                     /db_xref="RGD:620152"
     exon            1..109
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..3622
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /codon_start=1
                     /product="sperm-associated antigen 5"
                     /protein_id="NP_001037689.1"
                     /db_xref="GeneID:252918"
                     /db_xref="RGD:620152"
                     /translation="MWRVKTLNLGLSPSPQSGKPAMRTPLRELKLQPEALADSGKGPM
                     FSSLTPYLRRLDLKERRNSSSPVNFVNAENSSPVDFVNTENTSPVDFDNTENNFLSEQ
                     FSHPSEYIEACQRESDLTPESSSPFNTLEKAVEAMDDFVAEPIDDGIVKTMVLLPFSL
                     GQQQDLMLQAHLDTTAERNKSSLKESLGLEDLVEKEAAPCVEDSLPETVAIRPEQPIS
                     QDPPLRDSNTEAAPAGSVPSENVLGFTVACLSPSTVLTKDFSVDHVDPGEETAENRVI
                     QEVEKSFLALPEEAGLGDQALAVNAEDVSSLYLSSSLVEMGPQEAPGPTAEDASRIPD
                     LESETWMSPLAWLEKGVNTSVMLQSLRQSLPFSKSVLQDAAVGNTPHSTYSVGTSFTP
                     PVPLEVGTKDSTSETERLLLGCRPPDLAALSRHDLEENLLNALVLLEVLSHQLQAWKS
                     QLTVPSRQAQDSSTQTDSSAAVVTKTPKHLQDSKEIRQALLQARNVIHSWGLVSRDLM
                     SLLHLSLTHVQDDRVTVSQESQRAETLVSSCSRVLKKLRAKLQSLKTEWEEARHREEM
                     ALKGKDAAEAVLEAFRAHASQRISQLEQGITSVQEFRGLLQEAQTQLIGLHTEQEELA
                     QHTVSLTSALQQDWTSVQQNYGTWAALLSRSRELTKKLTAKSRQALQERDAAIEEKDQ
                     VVKEVEQVSAHLEDCKGQIEQLKLENSRLTADLSAQLQTLASTENELKELQSQHSHCV
                     QDLAMKDELLCQLTQSNKEQAAQWQKEETELKHRQAELLQQKAVLAKEVQDLRETMEF
                     VDEESQVAHLELGQIESQLKATLEVLRERSLQCETLKDTVESLRAELASTEAKHEQQA
                     LEKTHQHSKELCLLAEQLQSLTLFLEAKLEENKAESDIILPSTGCAPAQEHPPSSDSS
                     VSEQIPTAVVDEVPEPAPVPLLGSVKSAFTRVASTAPFRPTETPALEKSLAEMSAVLQ
                     ELQRLCALLQESKEEAVGVLQREICELHTRLQAQEEEHQEAQKAKEADIEKLNQALCL
                     RHKNEKELLEVIQKQNEKILGQIDTSGQLINLREEVTQLTRLLRRAETETKVLQEALE
                     GQVDPSCQLMATNWIQEKVFLSQEVNKLRAMFLEVKNEKKQLMDKYLSHRHILEENLR
                     RSDTELKKLDDTIQHIYETLLSIPEVVKSCKELQGLLEFLS"
     exon            110..232
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            233..1296
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1474
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1549
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            1550..1642
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1777
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1897
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            1898..1990
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2104
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2198
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            2199..2346
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2449
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            2450..2583
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            2584..2725
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            2726..2837
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..2924
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            2925..3045
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            3046..3157
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            3158..3238
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            3239..3394
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            3395..3469
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            3470..3550
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
     exon            3551..3863
                     /gene="Spag5"
                     /gene_synonym="Deepest"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agaggcggcg ggtgaatgtg gcgttttcga cttggtcaga taagtggtct aattaaagat
       61 gtggagggtg aaaacactga atctcggctt gtcgccttcg ccccaatcgg ggaaaccagc
      121 tatgagaact cctctccgag agcttaaact acagcccgag gccctcgctg attcagggaa
      181 aggtcctatg ttctcctcgc tgaccccata cctgcgcagg ctggatctga aggaaaggcg
      241 caacagctca tctccagtga attttgttaa tgcagagaac tcatctccag tggattttgt
      301 caatactgag aacacatctc cagtggattt tgacaatact gagaacaact tcctttcaga
      361 acagttcagc catccttcag agtacataga agcttgtcag cgtgaatcag atctaactcc
      421 tgaaagcagc tctcctttca acacactgga gaaggcagtg gaagcaatgg atgattttgt
      481 ggcagaacca atagatgacg gtatagttaa gaccatggtt cttttaccct tttccttagg
      541 gcagcagcaa gacctgatgc tccaggctca cttagatacc acagcagaga gaaataaaag
      601 ctctctaaaa gaatctttgg ggctagaaga cctggtggaa aaggaggcgg caccttgtgt
      661 ggaagacagc ttaccagaaa ctgttgctat caggcctgag caacccatct ctcaggaccc
      721 tccattaaga gacagtaaca ctgaagctgc acctgcgggc tcagttcctt ctgaaaatgt
      781 cttgggtttc actgtggctt gtctctctcc ctcaactgtc ttgaccaaag atttctctgt
      841 cgatcatgtc gatcctgggg aggaaactgc agagaacaga gttatacagg aagtggaaaa
      901 gagttttctc gcactccctg aggaggctgg attgggagat caggcacttg ctgtaaatgc
      961 agaagacgtc tcatccttat acctgtcatc aagtctagta gaaatgggac cccaggaggc
     1021 tccaggccca acagcagaag atgccagtag gattcctgac ttggagtcag agacttggat
     1081 gtccccattg gcctggctag aaaaaggtgt gaacacgtca gtcatgctac aaagtctccg
     1141 ccagagttta cccttttcaa aatctgtgct tcaggatgct gccgttggca atacacccca
     1201 ctccacttat tctgtgggaa cttcctttac tcctccagtg ccactggaag taggcaccaa
     1261 ggacagtact tcagagacag agcgcctcct cttgggctgc cgtcctccag atctggcagc
     1321 cttgtcccgg cacgacttgg aagagaacct gttgaacgcc cttgtcttgc tggaagttct
     1381 ttctcaccag ctgcaagcct ggaagagtca gctgactgtc ccgagccggc aagctcaaga
     1441 cagcagcaca cagaccgaca gctctgcagc tgtggtcact aagacaccta aacatcttca
     1501 ggacagcaag gagattcgac aggctctact gcaagccagg aatgtcatac attcatgggg
     1561 gctagtctct agagacctga tgtccttgct tcacctgtcc ctgacacatg tgcaagatga
     1621 cagagtgaca gtgagtcagg agtctcagcg tgcagagacc ctggtttcct cctgttctcg
     1681 tgtactgaag aaattgaggg caaagctcca gagcctcaaa acagaatggg aggaggcacg
     1741 gcacagggag gaaatggccc ttaaaggcaa ggatgcggca gaggcagtac tggaggcttt
     1801 ccgtgcacac gctagccagc gcatcagcca actggaacag ggtatcacat ctgtgcagga
     1861 attcagaggc cttctacagg aggcacagac ccaactgata gggcttcaca cagagcaaga
     1921 agagctggct cagcacacag tgagtctgac ttctgccttg cagcaggact ggacatcagt
     1981 ccaacagaat tatgggacat gggcagcttt gctgagtcgg tctcgagaac tcaccaagaa
     2041 actcacagcc aagagccggc aagcccttca ggaacgtgat gctgcaattg aggaaaagga
     2101 tcaggttgtg aaggaagtgg aacaagtctc tgcccattta gaggactgta aaggccaaat
     2161 agaacaactg aagttggaaa atagtcgcct tactgcagat ctctcggctc agctgcagac
     2221 tctggctagc acagagaacg aactaaagga actacagagt cagcattccc attgtgtcca
     2281 agacttggcc atgaaggatg aactgctgtg tcaacttacc cagagcaaca aggaacaggc
     2341 tgctcagtgg caaaaggaag aaacggaact gaaacacaga caagcagaac tgctgcagca
     2401 gaaggctgtg ttggctaagg aggtacagga cctgagggag accatggagt ttgtagatga
     2461 agagagtcaa gttgctcacc tggagctggg ccagatcgag agtcagttga aagccacact
     2521 agaagttctg cgggagcgca gcctgcagtg tgagaccctc aaggacaccg tggagagcct
     2581 gagggcagag ctggccagca ccgaagcaaa gcatgagcaa caagcgctgg agaagacgca
     2641 ccagcattct aaagagctgt gtctattggc ggagcagcta cagagtctca ccctcttctt
     2701 agaggctaaa ctagaggaga acaaggcaga atcagacatc attctgccca gcacaggctg
     2761 tgctccagcc caggaacacc ctccttccag tgatagcagc gtctcagaac agatcccgac
     2821 agcagtggta gatgaagtgc cagaaccagc tcctgtgcca ttgcttggaa gtgttaagag
     2881 tgctttcacc cgcgtagcct caacagctcc ctttcggcct acagagaccc cagccctgga
     2941 gaagagcctg gcagaaatga gtgctgtgtt acaggagctt caacgcctct gtgccctgct
     3001 gcaagaatct aaagaggaag ccgttggtgt cctgcagagg gaaatctgtg aactgcacac
     3061 aagactacag gcccaagaag aagaacatca ggaagcccaa aaggcaaagg aagcagacat
     3121 agagaagttg aaccaggcct tgtgcttgcg ccacaagaat gagaaggaac tcctggaagt
     3181 gatacagaag cagaacgaaa agatcctggg gcagatagac acgagtggcc agctcataaa
     3241 cctcagggag gaggtgaccc agcttacacg tttacttcgg cgggcagaga cagagactaa
     3301 agtgctccag gaagccctgg aaggccaggt agatcccagc tgtcagctga tggctactaa
     3361 ctggatccag gaaaaagtgt ttctctcgca ggaggtgaac aaactgaggg ctatgttcct
     3421 ggaggtgaaa aatgagaaga aacagctgat ggacaagtat ctgagccata ggcacatcct
     3481 ggaggagaat cttcggcgct ctgacacaga gttaaagaaa cttgatgaca caattcagca
     3541 tatctatgag actctgttgt ctatcccaga ggtagtgaag agttgcaagg agctacaagg
     3601 attgctagaa tttctgagct aagaaagaag atactggaag gcagtggtgt gtacctgtag
     3661 tcccagctgc tccagaggcc gaagctgagg accgcctaat cccaggaggt tgggactaac
     3721 ctgacatgga caacacagca aaaaacctgg aagataattc cactccactc tggtttcctt
     3781 caggatccct tatcccaata ataaaccaat tggcatggag ccgactatgt tttatgctat
     3841 ttaaataaag tgtatttaat gtaaaaaaaa aaaaaaaaaa a
//
