LOCUS       NM_001012116            3681 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus sperm associated antigen 1 (Spag1), mRNA.
ACCESSION   NM_001012116 XM_235372
VERSION     NM_001012116.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Neesse,A., Gangeswaran,R., Luettges,J., Feakins,R., Weeks,M.E.,
            Lemoine,N.R. and Crnogorac-Jurcevic,T.
  TITLE     Sperm-associated antigen 1 is expressed early in pancreatic
            tumorigenesis and promotes motility of cancer cells
  JOURNAL   Oncogene 26 (11), 1533-1545 (2007)
   PUBMED   16983343
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Lin,W., Zhou,X., Zhang,M., Li,Y., Miao,S., Wang,L., Zong,S. and
            Koide,S.S.
  TITLE     Expression and function of the HSD-3.8 gene encoding a
            testis-specific protein
  JOURNAL   Mol Hum Reprod 7 (9), 811-818 (2001)
   PUBMED   11517287
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC085828.1.
            
            On Feb 11, 2005 this sequence version replaced XM_235372.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085828.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q22"
     gene            1..3681
                     /gene="Spag1"
                     /note="sperm associated antigen 1"
                     /db_xref="GeneID:315033"
                     /db_xref="RGD:1310702"
     exon            1..287
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            288..435
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     CDS             290..2971
                     /gene="Spag1"
                     /note="HSD-3.8; infertility-related sperm protein Spag-1"
                     /codon_start=1
                     /product="sperm-associated antigen 1"
                     /protein_id="NP_001012116.1"
                     /db_xref="GeneID:315033"
                     /db_xref="RGD:1310702"
                     /translation="MTAKVKDHPPLWGFGTTKTFKIPIEHLDFKYIENCSDVKQLEKI
                     LCVLRSGEEGYYPELTEFCEKRLTGLAPRSRALRKDKPAATASSFSAEEWEKIDSDLK
                     SWVSEIKREENTRHFHDPEKHPGVEDPLPPVRGSNSCPRGGKETSSKSKTAKKRIPRD
                     YAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIDTAGLTEKEKNFLANREKG
                     KGNEAFYSGDYEEAVMYYTRSLSALPTATAYNNRAQAEIKLQRWSSALEDCEKALELE
                     PGNIKALLRRATTYKHQNKFLEAVDDLRKVLQAEPDNDLAKKTLSEVERELKNSEPAS
                     ELQTKGKRMVIEEVENSGDEGGKGDEDDHEDDGVDMAAMGNIQKKLMVRSQGGRRSRR
                     ARTPMPGAEQQEGQPETGTASTSDNHDLEERRAADSPGDLKSRGNELFRGGQFAEAAV
                     QYSGAIAQLEPTGSENADELSILYSNRAACYLKEGNCRGCIQDCDRALELQPFAVKPL
                     LRRAMAYETLEQYRSAYVDYITVLKIDCRIQLASDSVNRITRILTELDGPKWRERLPP
                     IPAVPASEPLRVWHPAAETPDQDPCPNSCTPTITDEKMFQALKEEGNQLVKDKNYKDA
                     ISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCDKALQIDSKNVKASYRLELA
                     QKGLENCRERVADPSQVVLLSPDSSEAARHLDTKNDTAPPSRERERRRIEIQEVDDSS
                     DEEPERPAEASAVEEGWSAERAGKIEVCKPRNAYEFGQVLSTISARKDEEACAQLLVF
                     TAPQDLPVLLSNKLEGDMLLLIMQSLKSHLVAKDPSLVCKHLLYLSKAERFEMMLALT
                     SKDQKEQMAQLFDDLSDAQADCLTAEDIQALRRQYVL"
     exon            436..595
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            596..721
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            722..786
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            787..896
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            897..1002
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1133
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1240
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1400
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1676
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1776
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1929
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2090
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            2091..2223
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..2350
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            2351..2494
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            2495..2839
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
     exon            2840..3651
                     /gene="Spag1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgtgttaagc actggctccg gttctcgggt cgctctccgc tggtcatagt cagagacccc
       61 ggtgagcacc cgcggggcgt ggaggaagcg ggccgcacac gcacactatc gatgaacgtc
      121 ctttctggtc tggaaaggtg attggaactt cccaccgctg tggttcgcac gggagtggaa
      181 attgtggtgg agttgccgca ctgcggtttc ctgggacagc catctgcttc agcccgggga
      241 acctcagccc aggccagagg aacgactccc gacgccacgg cttgcagcca tgactgctaa
      301 ggttaaggac catccaccac tgtggggctt tggaacaaca aaaacattta aaattcccat
      361 cgaacatttg gatttcaagt atattgaaaa ctgttcagat gtgaaacagc tggaaaaaat
      421 tctttgtgtg ctaaggtctg gtgaagaagg ctattatcct gaactcacgg agttttgtga
      481 gaagcgcctc accggcttgg ctcccagaag tcgagctctg aggaaggaca agcctgcagc
      541 cacagcctcc agcttttcag cagaagagtg ggaaaaaatc gacagcgatc taaagagttg
      601 ggtgtcagaa atcaaaagag aggaaaacac acgacacttc catgaccctg aaaaacatcc
      661 aggagtggaa gatcctctgc ctcctgttcg tggctccaac agctgtcctc gcggcggcaa
      721 ggaaacatct tctaaaagta agacagctaa gaagaggatt ccaagggatt atgcagagtg
      781 ggataaattt gatgtggaaa aagagtgttc aaagattgat gaggattaca aagagaagac
      841 tgtcataaac aacaaagctc atttgtctaa aattgagacg aaaatagaca cagcaggtct
      901 gactgagaag gaaaagaatt ttcttgccaa ccgtgaaaag gggaaaggga acgaggcgtt
      961 ctactcagga gactacgaag aggctgtgat gtattacacc aggagcttat ccgctcttcc
     1021 cactgcgact gcctacaaca atcgtgctca ggccgaaatc aagctgcaga gatggagcag
     1081 tgctcttgaa gactgcgaga aggccttgga gctggagcct gggaacataa aagctctcct
     1141 gagacgcgcc accacatata aacatcaaaa caagttcctg gaggccgtgg acgacctgag
     1201 aaaggtgctg caagcagagc ctgacaacga cctggccaag aaaaccttat cagaggttga
     1261 aagagagctg aagaattcag agcctgcctc tgagctccaa accaaaggga agaggatggt
     1321 tattgaggaa gttgaaaact cgggagatga aggtgggaag ggagacgaag atgatcatga
     1381 agatgatggt gtggatatgg cggccatggg caacatccag aagaagctga tggttcggag
     1441 ccagggtggc aggcggtcgc ggcgggcccg gacaccgatg cccggagcgg agcagcagga
     1501 aggccagcca gagacaggga cggccagtac cagcgacaac cacgacctgg aggagcggcg
     1561 agccgcagac agtcccggcg acctcaagag ccgcggcaac gagctgttcc gcggtgggca
     1621 gttcgctgag gcagccgtac agtactcagg ggcgatagcg cagctggaac ccacaggaag
     1681 tgaaaatgcg gatgagctaa gcatcttgta ttcaaataga gcggcgtgct acctcaaaga
     1741 aggaaactgc aggggctgca tccaggactg tgacagggct ctggaacttc agccgttcgc
     1801 agtgaagcct cttttacggc gagcgatggc ctatgagact ttagagcagt acaggagcgc
     1861 gtatgtggac tatataacag tgctgaagat agactgtagg atccagctgg ccagcgacag
     1921 cgttaacaga ataacaagaa tcttaacaga gctagatgga ccaaaatggc gggagagact
     1981 gccccccatc ccggctgtgc ctgcatctga gcctctgaga gtttggcacc ctgctgctga
     2041 gaccccagac caagatccgt gtcccaacag ctgcacgccc accatcacag atgaaaaaat
     2101 gtttcaagcc cttaaggaag aaggaaatca acttgtaaag gacaaaaatt ataaagacgc
     2161 cattagtaaa tacaacgaat gcttaaagat taacagcaag gcatgtgcca tctatacaaa
     2221 cagggctctc tgttacttga agctgggaca gtttgaagag gcaaagctgg actgtgacaa
     2281 ggcacttcag atagacagca agaatgtgaa agcctcttac agactcgagc tcgcccagaa
     2341 aggactcgag aattgccggg aaagagtggc ggatcccagt caggttgtcc tcctaagccc
     2401 agattccagt gaagcagcca ggcacctgga cactaagaat gacacagcac ctcccagcag
     2461 agagagggag aggaggagaa ttgaaatcca agaggtggat gacagcagtg atgaggagcc
     2521 tgagcggccc gcagaggcct ctgccgtgga agaggggtgg tctgcagaac gagccgggaa
     2581 gatcgaggtg tgcaagccta ggaacgccta tgagttcggg caggtcctga gcactatcag
     2641 cgccaggaaa gacgaagagg cctgtgcaca gcttttagtc ttcactgctc cccaagacct
     2701 gcctgtgttg ctaagtaaca aactggaagg ggacatgctc ctcctcatca tgcagtcact
     2761 gaaaagtcac cttgtggcca aggacccctc actggtgtgc aagcatctcc tgtatctgag
     2821 caaagcagag aggtttgaga tgatgctggc actgactagc aaagaccaaa aggagcagat
     2881 ggcgcagctg tttgatgacc tgtcagacgc acaggctgac tgtctcacgg cagaggatat
     2941 acaggcccta agaaggcagt atgtgcttta actcgggtgc tgagttcaaa gcagtctctg
     3001 atggagttgc atgggtaaag ccacacccag aaggagccac acccagaagg acccatttgg
     3061 acatcagacg ttatacttat ttttgtatcc agaacatgta tattctataa tctgattctt
     3121 atttattgta aatattctct tctgtactag agccaatacc tttatattta ataactatat
     3181 ttggaacttg gcaaacacta atttataaaa cattttcttt taatgaccta cccataaaaa
     3241 tttaaacaca ttcttcattg tggcaattct ttcaggtttg acagaaccca ggtcttccgt
     3301 tctttaaaag caaatgttag cactgataac tccatcagat gcacaaaatc tactttagag
     3361 attgaaacaa cacttgcatc tttgaggtca ttattagctt attaacttag agccctaagg
     3421 gagttatttt tctctaatat ttttactgtg actttcttct tgtttgtaaa cctttcaccc
     3481 tacctttgta tttgaattgc caccctagcc aatagttgct attcacacgc ccttcccaat
     3541 ctgttttctg cttggtttct ttactgtaac aagtgagaag tcactggatg ttcccttttc
     3601 tgggttaaag gtattcccta tgcaataaac aaacatccaa gccttaattt aaaaaaaaaa
     3661 aaaaaaaaaa aaaaaaaaaa a
//
