LOCUS       NM_030991               2135 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Rattus norvegicus synaptosome associated protein 25 (Snap25),
            transcript variant 3, mRNA.
ACCESSION   NM_030991 XM_346670
VERSION     NM_030991.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Hu,A., Lan,H., Yao,Z. and Kong,X.
  TITLE     Carbohydrate response element-binding protein (ChREBP) mediates
            decreased SNAP25 expression in islets from diabetic Goto-Kakizaki
            (GK) rats
  JOURNAL   FEBS Open Bio 14 (11), 1864-1872 (2024)
   PUBMED   39300600
  REMARK    GeneRIF: Carbohydrate response element-binding protein (ChREBP)
            mediates decreased SNAP25 expression in islets from diabetic
            Goto-Kakizaki (GK) rats.
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Polishchuk,A., Cilleros-Mane,V., Balanya-Segura,M., Just-Borras,L.,
            Fornies-Marine,A., Silvera-Simon,C., Tomas,M., Jami El Hirchi,M.,
            Hurtado,E., Tomas,J. and Lanuza,M.A.
  TITLE     BDNF/TrkB signalling, in cooperation with muscarinic signalling,
            retrogradely regulates PKA pathway to phosphorylate SNAP-25 and
            Synapsin-1 at the neuromuscular junction
  JOURNAL   Cell Commun Signal 22 (1), 371 (2024)
   PUBMED   39044222
  REMARK    GeneRIF: BDNF/TrkB signalling, in cooperation with muscarinic
            signalling, retrogradely regulates PKA pathway to phosphorylate
            SNAP-25 and Synapsin-1 at the neuromuscular junction.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2135)
  AUTHORS   Kong,X., Lin,C., Yang,C., Wang,X., Li,B., Yan,D., Yang,Y., Hu,A.,
            Chen,Y., Xu,X. and Ma,X.
  TITLE     GLP-1 signaling-regulated SNAP25 is involved in improved insulin
            secretion in diabetic GK rats after Roux-en-Y gastric bypass
            surgery
  JOURNAL   Mol Biol Rep 51 (1), 123 (2024)
   PUBMED   38227062
  REMARK    GeneRIF: GLP-1 signaling-regulated SNAP25 is involved in improved
            insulin secretion in diabetic GK rats after Roux-en-Y gastric
            bypass surgery.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2135)
  AUTHORS   Si,W., Sun,B., Luo,J., Li,Z., Dou,Y. and Wang,Q.
  TITLE     Snap25 attenuates neuronal injury via reducing ferroptosis in acute
            ischemic stroke
  JOURNAL   Exp Neurol 367, 114476 (2023)
   PUBMED   37393984
  REMARK    GeneRIF: Snap25 attenuates neuronal injury via reducing ferroptosis
            in acute ischemic stroke.
REFERENCE   5  (bases 1 to 2135)
  AUTHORS   Chen,J., Wang,Z., Wang,T., Cheng,J., Zhuang,R. and Wang,W.
  TITLE     SNAP23 decreases insulin secretion by competitively inhibiting the
            interaction between SNAP25 and STX1A
  JOURNAL   Biosci Rep 43 (5) (2023)
   PUBMED   37057886
  REMARK    GeneRIF: SNAP23 decreases insulin secretion by competitively
            inhibiting the interaction between SNAP25 and STX1A.
REFERENCE   6  (bases 1 to 2135)
  AUTHORS   McMahon,H.T., Missler,M., Li,C. and Sudhof,T.C.
  TITLE     Complexins: cytosolic proteins that regulate SNAP receptor function
  JOURNAL   Cell 83 (1), 111-119 (1995)
   PUBMED   7553862
REFERENCE   7  (bases 1 to 2135)
  AUTHORS   Hata,Y. and Sudhof,T.C.
  TITLE     A novel ubiquitous form of Munc-18 interacts with multiple
            syntaxins. Use of the yeast two-hybrid system to study interactions
            between proteins involved in membrane traffic
  JOURNAL   J Biol Chem 270 (22), 13022-13028 (1995)
   PUBMED   7768895
REFERENCE   8  (bases 1 to 2135)
  AUTHORS   Chilcote,T.J., Galli,T., Mundigl,O., Edelmann,L., McPherson,P.S.,
            Takei,K. and De Camilli,P.
  TITLE     Cellubrevin and synaptobrevins: similar subcellular localization
            and biochemical properties in PC12 cells
  JOURNAL   J Cell Biol 129 (1), 219-231 (1995)
   PUBMED   7698987
REFERENCE   9  (bases 1 to 2135)
  AUTHORS   Garcia,E.P., McPherson,P.S., Chilcote,T.J., Takei,K. and De
            Camilli,P.
  TITLE     rbSec1A and B colocalize with syntaxin 1 and SNAP-25 throughout the
            axon, but are not in a stable complex with syntaxin
  JOURNAL   J Cell Biol 129 (1), 105-120 (1995)
   PUBMED   7698978
REFERENCE   10 (bases 1 to 2135)
  AUTHORS   Hess,D.T., Slater,T.M., Wilson,M.C. and Skene,J.H.
  TITLE     The 25 kDa synaptosomal-associated protein SNAP-25 is the major
            methionine-rich polypeptide in rapid axonal transport and a major
            substrate for palmitoylation in adult CNS
  JOURNAL   J Neurosci 12 (12), 4634-4641 (1992)
   PUBMED   1281490
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from FQ214162.1 and BC087699.1.
            
            On Jul 19, 2012 this sequence version replaced NM_030991.2.
            
            Summary: Synaptic vesicle membrane docking and fusion is mediated
            by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment
            protein receptors) located on the vesicle membrane (v-SNAREs) and
            the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE
            complex consists of a bundle of four helices, one of which is
            supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on
            the plasma membrane, the protein syntaxin supplies one helix and
            the protein encoded by this gene contributes the other two.
            Therefore, this gene product is a presynaptic plasma membrane
            protein. It is essential for regulated exocytosis in neuronal
            cells. Alternative transcript variants encoding two different
            protein isoforms have been described for this gene. [provided by
            RefSeq, Jul 2012].
            
            Transcript Variant: This variant (3, also known as b) has an
            alternate in-frame exon in the coding region, compared to variant
            1. The resulting isoform (b) is of the same length, but has a
            distinct internal segment, compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132261,
                              SAMD00132262 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                FQ214162.1         1-45
            46-2135             BC087699.1         1-2090
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q36"
     gene            1..2135
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
                     /note="synaptosome associated protein 25"
                     /db_xref="GeneID:25012"
                     /db_xref="RGD:3728"
     exon            1..147
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
                     /inference="alignment:Splign:2.1.0"
     exon            148..281
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..830
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
                     /note="isoform b is encoded by transcript variant 3;
                     synaptosomal-associated protein, 25 kDa; SUP; SNAP-25;
                     super protein; synaptosomal-associated 25 kDa protein;
                     synaptosomal-associated protein 25"
                     /codon_start=1
                     /product="synaptosomal-associated protein 25 isoform b"
                     /protein_id="NP_112253.1"
                     /db_xref="GeneID:25012"
                     /db_xref="RGD:3728"
                     /translation="MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGI
                     RTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYK
                     KAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNL
                     RHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG"
     exon            282..323
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
                     /inference="alignment:Splign:2.1.0"
     exon            324..372
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
                     /inference="alignment:Splign:2.1.0"
     exon            373..490
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
                     /inference="alignment:Splign:2.1.0"
     exon            491..616
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
                     /inference="alignment:Splign:2.1.0"
     exon            617..761
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
                     /inference="alignment:Splign:2.1.0"
     exon            762..2104
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2085..2090
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
     polyA_site      2104
                     /gene="Snap25"
                     /gene_synonym="SNAP-25a; SNAP-25B"
ORIGIN      
        1 atctttgatg agggcagagc tcacgttgca ttgaagacga aacctcgggg aggtctggag
       61 ctgtctttcc ttccctccct acccggcggc tcctccactc ttgctacctg cagggatcag
      121 cggacagcat cctctgaaga agacaaggtt ccttaactaa gcaccactga cttgctggcc
      181 ccggcgccca gcaacccccc accactacca tggccgagga cgcagacatg cgtaatgaac
      241 tggaggagat gcagaggagg gctgaccagc tggctgatga gtccctggaa agcacccgtc
      301 gcatgctgca gctggtcgaa gagagtaaag atgctggcat caggactttg gttatgttgg
      361 atgagcaagg cgaacaactg gaacgcattg aggaagggat ggaccaaatc aataaggata
      421 tgaaagaagc agaaaagaat ttgacggacc taggaaaatt ctgcgggctt tgtgtgtgtc
      481 cctgtaacaa gcttaaatcc agtgatgctt acaaaaaagc ctggggcaat aatcaggatg
      541 gagtagtggc cagccagcct gcccgtgtgg tggatgaacg ggagcagatg gccatcagtg
      601 gtggcttcat ccgcagggta acaaacgatg cccgggaaaa tgaaatggat gaaaacctag
      661 agcaggtgag cggcatcatc ggaaacctcc gtcatatggc cctagacatg ggcaatgaga
      721 ttgacaccca gaatcgccag attgacagga tcatggagaa ggctgactcc aacaaaacca
      781 gaattgatga agccaaccaa cgtgcaacaa agatgctggg aagtggttaa atctgccctt
      841 ctgctgtgct ctcctccaat gttgttggac aagagagaag agagctcctt catgcttctc
      901 tcatggtatt acctagtaag acttacacac acacacacac acacacacac acacacacac
      961 acacacacac acagagtcag tcacccccat tgtaaatgtc ctgtgtggtt tgtcagcttc
     1021 ccaatgatac catgtgtctt ttgttttctc cggctctctt tctttccaaa ggttgtacat
     1081 agtggtcatc tggtgactct atttcctgac ttaagatgtc ttgggtctct ctctttttcc
     1141 tttttctttt ctcagtggcg tttgctgaat gacaacaatt taggaatgct caatgtactg
     1201 ttgatttttc tcaatacaca gtattgttct tgtaaaactg tgacattcca cagagctact
     1261 accacagtcc tttcttaggg tgtcaggctc tgaatctctc caaatgtgct gtctttggtt
     1321 cctcatggct attctttgtc tttatgattt cataattaga caatgtgaaa ttacataaca
     1381 ggcattgcac taaaagtgat gtgatttatg catttatgca tgagaactaa atagactttt
     1441 agattcctac ttaaacaaaa actttccatg acagtagcat actgacaaga aaacacacac
     1501 aacagcaaca ataacaaagc aacaactacg catgctcagt attgggacac tgtcaagatt
     1561 aagtcatacc agcaaaacct gcagctgtgt caccttcttc tgtcaacata cagactgatc
     1621 ataatcatcc cttctttaca cacacacaca cacacacaca cacacacaca cacacacaca
     1681 caaatggatt taacacacca ttaatcattt ctagcaaaat atatgtttgg ctgaaactat
     1741 gtgaaatggg tgtaatatag ggtttgtcga atgcttttga aagctctgtt ttggagacaa
     1801 tactcttgtg gaaaacgtga agatcttcta agtctggctc ttgttgatca ccatttccct
     1861 gtggtttgtc atcagtacaa ctctttgttg cttaatctag agctatgcac accaaattgc
     1921 tgagatgttt agtagctgat aaagaaacct ttaaaaaatt atataaatga atgaaatata
     1981 gataaactgt gagataaata tcattacagc atgtatatta aatccctcct gtctcctctg
     2041 ttggtttgtg aagtgatttg acattttgta gctagtttaa aattattaaa aattatagat
     2101 cccaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa
//
