LOCUS       NM_001109598            5101 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus SMAD specific E3 ubiquitin protein ligase 1
            (Smurf1), mRNA.
ACCESSION   NM_001109598 XM_001074660
VERSION     NM_001109598.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Niu,H., Bi,F., Zhao,W., Xu,Y., Han,Q., Guo,W. and Chen,Y.
  TITLE     Smurf1 regulates ameloblast polarization by ubiquitination-mediated
            degradation of RhoA
  JOURNAL   Cell Prolif 56 (4), e13387 (2023)
   PUBMED   36579844
  REMARK    GeneRIF: Smurf1 regulates ameloblast polarization by
            ubiquitination-mediated degradation of RhoA.
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Qi,Y., Tang,Y., Yin,L., Ding,K., Zhao,C., Yan,W. and Yao,Y.
  TITLE     miR-129-5p restores cardiac function in rats with chronic heart
            failure by targeting the E3 ubiquitin ligase Smurf1 and promoting
            PTEN expression
  JOURNAL   Bioengineered 13 (2), 2371-2386 (2022)
   PUBMED   35034538
  REMARK    GeneRIF: miR-129-5p restores cardiac function in rats with chronic
            heart failure by targeting the E3 ubiquitin ligase Smurf1 and
            promoting PTEN expression.
REFERENCE   3  (bases 1 to 5101)
  AUTHORS   Bian,C., Luan,Z., Zhang,H., Zhang,R., Gao,J., Wang,Y., Li,J. and
            Ren,H.
  TITLE     miR-154-5p Affects the TGFbeta1/Smad3 Pathway on the Fibrosis of
            Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase Smurf1
  JOURNAL   Oxid Med Cell Longev 2022, 7502632 (2022)
   PUBMED   35126820
  REMARK    GeneRIF: miR-154-5p Affects the TGFbeta1/Smad3 Pathway on the
            Fibrosis of Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase
            Smurf1.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5101)
  AUTHORS   Xiong,A., He,Y., Gao,L., Li,G., Weng,J., Kang,B., Wang,D. and
            Zeng,H.
  TITLE     Smurf1-targeting miR-19b-3p-modified BMSCs combined PLLA composite
            scaffold to enhance osteogenic activity and treat critical-sized
            bone defects
  JOURNAL   Biomater Sci 8 (21), 6069-6081 (2020)
   PUBMED   33000773
  REMARK    GeneRIF: Smurf1-targeting miR-19b-3p-modified BMSCs combined PLLA
            composite scaffold to enhance osteogenic activity and treat
            critical-sized bone defects.
REFERENCE   5  (bases 1 to 5101)
  AUTHORS   Huang,S., Li,Y., Wu,P., Xiao,Y., Duan,N., Quan,J. and Du,W.
  TITLE     microRNA-148a-3p in extracellular vesicles derived from bone marrow
            mesenchymal stem cells suppresses SMURF1 to prevent osteonecrosis
            of femoral head
  JOURNAL   J Cell Mol Med 24 (19), 11512-11523 (2020)
   PUBMED   32871042
  REMARK    GeneRIF: microRNA-148a-3p in extracellular vesicles derived from
            bone marrow mesenchymal stem cells suppresses SMURF1 to prevent
            osteonecrosis of femoral head.
REFERENCE   6  (bases 1 to 5101)
  AUTHORS   Yamashita,M., Ying,S.X., Zhang,G.M., Li,C., Cheng,S.Y., Deng,C.X.
            and Zhang,Y.E.
  TITLE     Ubiquitin ligase Smurf1 controls osteoblast activity and bone
            homeostasis by targeting MEKK2 for degradation
  JOURNAL   Cell 121 (1), 101-113 (2005)
   PUBMED   15820682
REFERENCE   7  (bases 1 to 5101)
  AUTHORS   Shi,Y. and Massague,J.
  TITLE     Mechanisms of TGF-beta signaling from cell membrane to the nucleus
  JOURNAL   Cell 113 (6), 685-700 (2003)
   PUBMED   12809600
  REMARK    Review article
REFERENCE   8  (bases 1 to 5101)
  AUTHORS   Suzuki,C., Murakami,G., Fukuchi,M., Shimanuki,T., Shikauchi,Y.,
            Imamura,T. and Miyazono,K.
  TITLE     Smurf1 regulates the inhibitory activity of Smad7 by targeting
            Smad7 to the plasma membrane
  JOURNAL   J Biol Chem 277 (42), 39919-39925 (2002)
   PUBMED   12151385
REFERENCE   9  (bases 1 to 5101)
  AUTHORS   Ebisawa,T., Fukuchi,M., Murakami,G., Chiba,T., Tanaka,K.,
            Imamura,T. and Miyazono,K.
  TITLE     Smurf1 interacts with transforming growth factor-beta type I
            receptor through Smad7 and induces receptor degradation
  JOURNAL   J Biol Chem 276 (16), 12477-12480 (2001)
   PUBMED   11278251
REFERENCE   10 (bases 1 to 5101)
  AUTHORS   Zhu,H., Kavsak,P., Abdollah,S., Wrana,J.L. and Thomsen,G.H.
  TITLE     A SMAD ubiquitin ligase targets the BMP pathway and affects
            embryonic pattern formation
  JOURNAL   Nature 400 (6745), 687-693 (1999)
   PUBMED   10458166
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474012.1.
            
            On Oct 4, 2007 this sequence version replaced XM_001074660.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132272,
                              SAMD00132291 [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12p11"
     gene            1..5101
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /note="SMAD specific E3 ubiquitin protein ligase 1"
                     /db_xref="GeneID:690516"
                     /db_xref="RGD:1594738"
     exon            1..77
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..2209
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /EC_number="2.3.2.26"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase SMURF1"
                     /protein_id="NP_001103068.1"
                     /db_xref="GeneID:690516"
                     /db_xref="RGD:1594738"
                     /translation="MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDG
                     SGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLS
                     NAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEG
                     TVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVR
                     GSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNC
                     DELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPS
                     EGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMK
                     MRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQIN
                     PDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPE
                     LHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYV
                     NWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLK
                     HCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHL
                     IDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE"
     exon            78..116
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            117..225
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            226..359
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            360..425
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            426..501
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            502..743
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            744..828
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            829..975
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1174
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1278
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1393
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1572
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1710
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1831
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..2033
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2109
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
     exon            2110..5101
                     /gene="Smurf1"
                     /gene_synonym="RGD1309707"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccggccggc cgctggcggg agatgtcgaa ccccgggacc cgtaggaacg gctccagcat
       61 caagatccgt ctgacagtat tgtgtgccaa gaaccttgca aagaaagact tcttcagact
      121 ccccgacccc tttgccaaga ttgttgtgga cggctctggg cagtgccact caaccgacac
      181 tgtgaaaaac accctggacc caaagtggaa ccagcactat gacctgtacg ttgggaaaac
      241 cgactcgata accatcagtg tgtggaacca caagaagatc cacaagaagc agggggctgg
      301 cttcctgggc tgcgtgcggc tgctctccaa tgccatcagc agattgaaag ataccggcta
      361 ccagcgtttg gatctatgca aactaaatcc ctcagatact gatgcagttc gtggccaaat
      421 agtggtcagt ttacagaccc gagacagaat aggtggtgga gggtcggtgg tggactgcag
      481 gggactgctg gagaacgaag gaacggtata tgaagactca ggccctggaa ggccgctcag
      541 ctgcctcatg gaggaacctg ccccgtatac agatggcacc ggtgcagcgg caggaggtgg
      601 gaactgcagg tttgtggagt ctccaagcca agatcagaga ctcctggtac agcgactccg
      661 aaatcctgag gttcgaggat ccctacagac accgcagaat cgaccacatg gccaccagtc
      721 accggagctg cctgaaggct acgagcaaag gacaacagtg cagggacaag tttacttttt
      781 gcacacgcag actggagtca gcacatggca cgaccccagg atccccagag accttaacag
      841 tgtgaactgc gatgaacttg ggccactgcc tccaggctgg gaagtcagaa gcacagtgtc
      901 gggaagaatc tattttgtag atcacaataa taggacaacc cagtttacag acccacggct
      961 tcaccacatc atgaatcacc agtgccaact caaggagccc agccagccgc tgcagttgcc
     1021 cagtgagggc tccgtggagg acgaggagct tcctgcccag agatatgaga gagatttagt
     1081 ccagaagctt aaagttctca gacatgagct ctctcttcag cagccccaag ctggtcattg
     1141 tcgcatagaa gtttccagag aagaaatatt tgaggagtca tatcgccaga tcatgaagat
     1201 gcgaccaaaa gacctgaaga agcgcctgat ggtgaagttc cgaggggagg aaggcttgga
     1261 ctatggtggg gtggctcggg agtggctgta tttgttgtgc catgaaatgt tgaatccgta
     1321 ttatggactc ttccagtatt ccacggacaa tatttacaca ctgcaaatca acccagattc
     1381 ttctatcaac cctgaccatc tgtcttactt ccactttgtg ggtcgcatca tgggtctggc
     1441 tgtgttccat ggacactaca taaacggggg cttcacagtt cctttctaca agcagctcct
     1501 gggaaaaccc atccagctgt cggatctgga gtcagtggac ccagaacttc acaagagctt
     1561 ggtgtggatt ctagagaatg acatcacgcc agtgctggat cacaccttct gcgtggagca
     1621 caatgctttt gggcggatcc ttcagcatga actgaaaccc aatggcagaa atgtgcctgt
     1681 cactgaggag aacaagaagg agtatgtgcg gttgtatgtg aactggaggt ttatgagagg
     1741 aatcgaagcc cagttcctag cgcttcagaa agggtttaac gaactcattc cccaacactt
     1801 actgaagccc tttgaccaga aggaactaga gctgataata ggcggcctgg ataagataga
     1861 cctgaatgac tggaagtcca acactcggct gaagcactgt gtggcagaca gcaacatcgt
     1921 caggtggttc tggcaggcgg tggagacctt cgatgaggag aggagagcca ggctcctgca
     1981 gtttgtgaca ggatccacac gagttcctct ccaaggcttc aaggctctgc aaggcgcggc
     2041 agggccccgg ctcttcacca tccacctgat tgacgccaac accgacaacc tgcccaaggc
     2101 ccacacctgc tttaatcgga ttgacatccc accctatgag tcctatgaga agctctatga
     2161 gaagctgctg acagcggtag aggagacctg tggctttgcc gtggagtgaa gagcagcccg
     2221 tggccacaga gtcttgctca caaccaccag acccaaagca tatggcgtct gcgcgcctcc
     2281 cgcatggcgg gcggaggcct gagattccag aaaccgaggg aaaaggctcg tctccctcct
     2341 cctttggaga aggcaggcca ggggactttc ataggtggct cccacccatt tattcctcct
     2401 ttattatagt tcgcccactc ctccatcacc catccaataa aacgcagcca ggtttcgccc
     2461 tcagcctcct tggcatggtg gtaaggctgt ggtgtctccg ggagcatcaa ccatggtcag
     2521 ggccaagctg ggccccgagg gtttgcgtgg cctctgtacc agctccccct ctaactgtag
     2581 ccgcaaagcc ccctttcaag ttcagcagct gccaatagct tactgatctc aaattccaga
     2641 ggcctcacag gcttgcagcc tgtccagata gccggtggat ctccaagcag gaggtaaagg
     2701 cagtgaatgc ttgctggaac tccactatgg taggagactc gaggcgatgt gttttcccct
     2761 ttgagaggta aaggaattgc aacagccttc actggaagcc tgggtacttt tcacagtgtc
     2821 ctggctaaaa ataaggtttt gcaggggaaa aaaatcttta aaagtacaat ttgctgtgtt
     2881 ccctacccag tttgaaatta tcacgtcatg gagctaagca aagaataacc aaaaccagcc
     2941 tctgacccct gggtccagct cagtttctgt gcctggggat ccagaggctc ctagctgtct
     3001 tgaaatggac tcacatatgg ggattgctaa gagctaaact aacagaacaa ttttgtcacc
     3061 gcagccattg ggtggaacag caggagaggc cgctgacctg gggtcccctg atgtagctcc
     3121 ccagggttgg tgctgtgcac agagcagggt gtggtggggc gcgtggcaac agggtggggg
     3181 ctttacctcc ctacgtggcc ctcttcccca tggcagcggt gatgctaaca gttctactca
     3241 gtttgcttgc tcagtctccg agcaaggcca aagccagcct aacacgtcta acagcacttc
     3301 cggtcgcaca cggcttcaca gacgttgact cttacagccg agcagctgga ctgagacagc
     3361 tgagctctgg tggaaggcag ggcagcagcc aggtttccat ttgtagtttt gttgtttttg
     3421 tttgtggtgg gggtgagaac agggtaattc attagcaaga catttcattg gtaccagggc
     3481 tcaggttcag ccacgagttc aagttgtatg gcagatgatc aggaaggtcc tgttgggaca
     3541 ctggcttttg tggtttttct gttgcttccc ctcaggctga gggtagggaa gtgtctgctg
     3601 cttctgtcca gtgtggtctg aagcagtgct gaggtgatag ggggagtgga ttcatagggc
     3661 acatacaccc cctaaccgaa gagatttgtc cagcttggcc actggcacta cagagcatga
     3721 gtcagctgca ctctgggttg ccagggtgag gatgctaggt agacctcact gcacaaggac
     3781 cgaacccacc ccacctccca ggccagactg gggccttatt agtctctcaa ttctgccttg
     3841 ccccagtagg gcttctgtaa ttcatctacc ttcaagcttc tgagggtgtt ctaaaaccca
     3901 gctccctcat gactcgtgga ttcaaaaggc gcaaggaagc ctgtgtccta agtctcagcc
     3961 agtccatagg aagcaaccag accccacaca gtgacacact ttctggagac cagaaacctt
     4021 gcctgttgcc ctggcaccgt ccagactgca gtgatgaagg acaccagggt tctgtgcttc
     4081 ggggatgtgt gcaggagtgt caggtcatac tgtttgtgcc tcgggagtga tgggggacaa
     4141 gccttaggag tcccgtgtca gatgccctca gaggaagatc cagaggctgg gcctttaggg
     4201 gccgactggc tggcacacat gccacctact aaggcttcta acagtcactg tgcctgcatt
     4261 gctcgtgggt tcccctcatt gccggttctg agtgtcacaa cctcccccag gtctgttcat
     4321 gcatagcact gtcgtctgtt ggtagcgctg cacttccttg tctgtctacc atggttaggc
     4381 ccttctgacg ctcctcaaag acactgcgtg gccggcccac ggcacagctc ctggttgccc
     4441 tgcttgggtc tgggtgaagc cctttcatgt cagacctcgg tcgtcctagt ggccaccatc
     4501 acccagatgc ttcaggagtc ctgctctcca ggctcccctc accagcggcc ggcagccaca
     4561 ctcagggacg cagcaaacac cactctgctc ggcagtgctt cagagaggac accaggctgc
     4621 cgcaatggtg tggcagcagg tcgccgtctc ctgctgtctg tgaggagact ctgccacaag
     4681 atgagacgct aacccctggc ctggcttggc cacaaggtca ggttcaggtc gggcgaacgc
     4741 tggaccgatc tgcagagaac acgcctcatc ctaccgcagc ctgggggcca cgtggcagcc
     4801 ttctgaaaca gtgtggattt ttaaactgtg tttataatgt attcttattt attttgtaag
     4861 gtcctgtttg aagtgctgct gctaccccta gttgctcccc cagtttttat tgcccattaa
     4921 cctgtgacag ttgtacacta atgttctatg tcagaatgga aagtatttaa agaaatgcta
     4981 gttctattta atgcagtcgt ggaaccagct ggaaacacaa aacgttgact gtacagcagg
     5041 ctggacccgg gaggtcaggt tcattttgtt acatatgcaa taaactcaca actttacttt
     5101 t
//
