LOCUS       NM_177934               1029 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus single-strand-selective monofunctional uracil-DNA
            glycosylase 1 (Smug1), mRNA.
ACCESSION   NM_177934
VERSION     NM_177934.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Masaoka,A., Matsubara,M., Hasegawa,R., Tanaka,T., Kurisu,S.,
            Terato,H., Ohyama,Y., Karino,N., Matsuda,A. and Ide,H.
  TITLE     Mammalian 5-formyluracil-DNA glycosylase. 2. Role of SMUG1
            uracil-DNA glycosylase in repair of 5-formyluracil and other
            oxidized and deaminated base lesions
  JOURNAL   Biochemistry 42 (17), 5003-5012 (2003)
   PUBMED   12718543
  REMARK    GeneRIF: This enzyme has a role in repair of 5-formyluracil and
            other oxidized and deaminated base lesions.
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Matsubara,M., Masaoka,A., Tanaka,T., Miyano,T., Kato,N., Terato,H.,
            Ohyama,Y., Iwai,S. and Ide,H.
  TITLE     Mammalian 5-formyluracil-DNA glycosylase. 1. Identification and
            characterization of a novel activity that releases 5-formyluracil
            from DNA
  JOURNAL   Biochemistry 42 (17), 4993-5002 (2003)
   PUBMED   12718542
REFERENCE   3  (bases 1 to 1029)
  AUTHORS   Kavli,B., Sundheim,O., Akbari,M., Otterlei,M., Nilsen,H.,
            Skorpen,F., Aas,P.A., Hagen,L., Krokan,H.E. and Slupphaug,G.
  TITLE     hUNG2 is the major repair enzyme for removal of uracil from U:A
            matches, U:G mismatches, and U in single-stranded DNA, with hSMUG1
            as a broad specificity backup
  JOURNAL   J Biol Chem 277 (42), 39926-39936 (2002)
   PUBMED   12161446
REFERENCE   4  (bases 1 to 1029)
  AUTHORS   Boorstein,R.J., Cummings,A. Jr., Marenstein,D.R., Chan,M.K., Ma,Y.,
            Neubert,T.A., Brown,S.M. and Teebor,G.W.
  TITLE     Definitive identification of mammalian 5-hydroxymethyluracil DNA
            N-glycosylase activity as SMUG1
  JOURNAL   J Biol Chem 276 (45), 41991-41997 (2001)
   PUBMED   11526119
REFERENCE   5  (bases 1 to 1029)
  AUTHORS   Shao,X., Xue,J. and van der Hoorn,F.A.
  TITLE     Testicular protein Spag5 has similarity to mitotic spindle protein
            Deepest and binds outer dense fiber protein Odf1
  JOURNAL   Mol Reprod Dev 59 (4), 410-416 (2001)
   PUBMED   11468777
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY191521.1.
            
            On Feb 21, 2013 this sequence version replaced NM_177934.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY191521.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1029              AY191521.1         6-1034
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q36"
     gene            1..1029
                     /gene="Smug1"
                     /note="single-strand-selective monofunctional uracil-DNA
                     glycosylase 1"
                     /db_xref="GeneID:315344"
                     /db_xref="RGD:631403"
     exon            1..181
                     /gene="Smug1"
                     /inference="alignment:Splign:2.1.0"
     exon            182..477
                     /gene="Smug1"
                     /inference="alignment:Splign:2.1.0"
     CDS             193..1029
                     /gene="Smug1"
                     /codon_start=1
                     /product="single-strand selective monofunctional
                     uracil-DNA glycosylase"
                     /protein_id="NP_808795.1"
                     /db_xref="GeneID:315344"
                     /db_xref="RGD:631403"
                     /translation="MAVSQTFPPGPAHEPASALMEPCARSLAEGFLEEELRLNAELSQ
                     LQFPEPVGVIYNPVDYAWEPHRNYVTRYCQGPKEVLFLGMNPGPFGMAQTGVPFGEVN
                     VVRDWLGIGGSVLSPPQEHPKRPVLGLECPQSEVSGARFWGFFRTLCGQPQVFFRHCF
                     VHNLCPLLFLAPSGRNLTPADLPAKHREQLLSICDAALCRQVQLLGVRLVVGVGRLAE
                     QRARRALAGLTPEVQVEGLLHPSPRSPQANKGWETAARERLQELGLLPLLTDEGSVRP
                     TP"
     exon            478..1029
                     /gene="Smug1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcatgcagcc atgacccacc atgatgaact gtactctctg agagctgaga taaacccttc
       61 cttctcctgc ctttacgggt tgcgttgtag ccacaagacc agtagctaag gtaggatgct
      121 tggtgtatca gaaagaggga ggactctgga gttgggaagc aaaaacatca ttccatacca
      181 ggtcacaacg gcatggctgt gtcccagact ttcccaccgg ggcctgccca tgagcctgcc
      241 agtgccctga tggagccttg cgctcgaagc ttagctgagg gcttcctgga ggaagagctt
      301 cggctcaatg ctgagctgag ccagctgcag tttccagagc ctgtgggtgt catctacaac
      361 ccagtggatt atgcttggga gccacaccgg aactacgtga ctcgctactg ccaaggcccc
      421 aaggaagtgc tgttcctggg catgaatcca ggaccttttg gcatggccca aactggggta
      481 ccttttgggg aagtgaatgt ggtccgggac tggttgggca ttgggggctc tgtgctgtcc
      541 cctccccaag agcaccctaa gcgaccagtg ttgggactgg agtgcccgca gtcagaggtg
      601 agcggagccc gattctgggg cttttttcgg accctctgcg gacagcctca agtcttcttc
      661 cggcactgct ttgtccacaa tctgtgtcct ctactcttct tggctcccag cgggcgaaac
      721 cttaccccgg ctgatctgcc cgccaagcac cgggagcagc tactgtcgat ctgcgacgcc
      781 gccctctgcc ggcaggtgca gctgctaggg gtgcggctgg tagtgggagt ggggcggctg
      841 gcagagcaga gagctcgaag agctctagca gggctgaccc cggaggtgca ggtggagggg
      901 ctcctgcatc catctcctcg aagcccgcag gccaacaaag gctgggaaac agcagccagg
      961 gaaagactcc aagagttggg gctgctgcct ctgctaacgg atgagggttc agtcaggcct
     1021 acaccatag
//
