LOCUS       NM_053605               5034 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), mRNA.
ACCESSION   NM_053605 XM_008772541
VERSION     NM_053605.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Silvera,S., Wilkinson,J.A. and LeBlanc,P.J.
  TITLE     Characterization of neutral sphingomyelinase activity and isoform
            expression in rodent skeletal muscle mitochondria
  JOURNAL   Mitochondrion 59, 184-189 (2021)
   PUBMED   34089907
  REMARK    GeneRIF: Characterization of neutral sphingomyelinase activity and
            isoform expression in rodent skeletal muscle mitochondria.
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Tan,L.H., Tan,A.J., Ng,Y.Y., Chua,J.J., Chew,W.S., Muralidharan,S.,
            Torta,F., Dutta,B., Sze,S.K., Herr,D.R. and Ong,W.Y.
  TITLE     Enriched Expression of Neutral Sphingomyelinase 2 in the Striatum
            is Essential for Regulation of Lipid Raft Content and Motor
            Coordination
  JOURNAL   Mol Neurobiol 55 (7), 5741-5756 (2018)
   PUBMED   29043558
  REMARK    GeneRIF: These results indicate an important function of nSMase2 in
            the striatum.
REFERENCE   3  (bases 1 to 5034)
  AUTHORS   Back,M.J., Ha,H.C., Fu,Z., Choi,J.M., Piao,Y., Won,J.H., Jang,J.M.,
            Shin,I.C. and Kim,D.K.
  TITLE     Activation of neutral sphingomyelinase 2 by starvation induces
            cell-protective autophagy via an increase in Golgi-localized
            ceramide
  JOURNAL   Cell Death Dis 9 (6), 670 (2018)
   PUBMED   29867196
  REMARK    GeneRIF: nSMase2 is a novel regulator of autophagy and provide
            evidence that Golgi-localized ceramides participate in
            cytoprotective autophagy against starvation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5034)
  AUTHORS   Dickens,A.M., Tovar-Y-Romo,L.B., Yoo,S.W., Trout,A.L., Bae,M.,
            Kanmogne,M., Megra,B., Williams,D.W., Witwer,K.W., Gacias,M.,
            Tabatadze,N., Cole,R.N., Casaccia,P., Berman,J.W., Anthony,D.C. and
            Haughey,N.J.
  TITLE     Astrocyte-shed extracellular vesicles regulate the peripheral
            leukocyte response to inflammatory brain lesions
  JOURNAL   Sci Signal 10 (473) (2017)
   PUBMED   28377412
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5034)
  AUTHORS   Li,J., Manickam,G., Ray,S., Oh,C.D., Yasuda,H., Moffatt,P. and
            Murshed,M.
  TITLE     Smpd3 Expression in both Chondrocytes and Osteoblasts Is Required
            for Normal Endochondral Bone Development
  JOURNAL   Mol Cell Biol 36 (17), 2282-2299 (2016)
   PUBMED   27325675
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5034)
  AUTHORS   Karakashian,A.A., Giltiay,N.V., Smith,G.M. and
            Nikolova-Karakashian,M.N.
  TITLE     Expression of neutral sphingomyelinase-2 (NSMase-2) in primary rat
            hepatocytes modulates IL-beta-induced JNK activation
  JOURNAL   FASEB J 18 (9), 968-970 (2004)
   PUBMED   15059969
REFERENCE   7  (bases 1 to 5034)
  AUTHORS   Won,J.S., Im,Y.B., Khan,M., Singh,A.K. and Singh,I.
  TITLE     The role of neutral sphingomyelinase produced ceramide in
            lipopolysaccharide-mediated expression of inducible nitric oxide
            synthase
  JOURNAL   J Neurochem 88 (3), 583-593 (2004)
   PUBMED   14720208
REFERENCE   8  (bases 1 to 5034)
  AUTHORS   Hofmann,K., Tomiuk,S., Wolff,G. and Stoffel,W.
  TITLE     Cloning and characterization of the mammalian brain-specific,
            Mg2+-dependent neutral sphingomyelinase
  JOURNAL   Proc Natl Acad Sci U S A 97 (11), 5895-5900 (2000)
   PUBMED   10823942
REFERENCE   9  (bases 1 to 5034)
  AUTHORS   Hayashi,Y., Kiyono,T., Fujita,M. and Ishibashi,M.
  TITLE     cca1 is required for formation of growth-arrested confluent
            monolayer of rat 3Y1 cells
  JOURNAL   J Biol Chem 272 (29), 18082-18086 (1997)
   PUBMED   9218439
REFERENCE   10 (bases 1 to 5034)
  AUTHORS   Fukuoka,S.I. and Scheele,G.
  TITLE     Complementary nucleotide sequence for monitor peptide, a novel
            cholecystokinin-releasing peptide in the rat
  JOURNAL   Nucleic Acids Res 17 (23), 10111 (1989)
   PUBMED   2602119
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On or before Dec 1, 2020 this sequence version replaced
            XM_008772541.2, NM_053605.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB000215.1, SRR26360194.1952086.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               JAXUCZ010000019.1  51155455-51155610   c
            157-1677            JAXUCZ010000019.1  51084416-51085936   c
            1678-1753           JAXUCZ010000019.1  51079375-51079450   c
            1754-1909           JAXUCZ010000019.1  51079118-51079273   c
            1910-1999           JAXUCZ010000019.1  51077248-51077337   c
            2000-2063           JAXUCZ010000019.1  51076994-51077057   c
            2064-2226           JAXUCZ010000019.1  51075257-51075419   c
            2227-5034           JAXUCZ010000019.1  51072209-51075016   c
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..5034
                     /gene="Smpd3"
                     /gene_synonym="cca1"
                     /note="sphingomyelin phosphodiesterase 3"
                     /db_xref="GeneID:94338"
                     /db_xref="RGD:619754"
     exon            1..156
                     /gene="Smpd3"
                     /gene_synonym="cca1"
                     /inference="alignment:Splign:2.1.0"
     exon            157..1677
                     /gene="Smpd3"
                     /gene_synonym="cca1"
                     /inference="alignment:Splign:2.1.0"
     CDS             361..2328
                     /gene="Smpd3"
                     /gene_synonym="cca1"
                     /EC_number="3.1.4.12"
                     /note="neutral sphingomyelin phosphodiesterase 3; nSMase2;
                     nSMase-2; sphingomyelin phosphodiesterase 3, neutral
                     membrane; neutral sphingomyelinase 2; neutral
                     sphingomyelinase II; confluent 3Y1 cell-associated protein
                     1; confluent 3Y1 cell-associated 1"
                     /codon_start=1
                     /product="sphingomyelin phosphodiesterase 3"
                     /protein_id="NP_446057.2"
                     /db_xref="GeneID:94338"
                     /db_xref="RGD:619754"
                     /translation="MVLYTTPFPNSCLSALHAVSWALIFPCYWLVDRLVASFIPTTYE
                     KRQRADDPCYLQLFCTVLFTPVYLALLVAALPFAFLGFIFWSPLQSARRPYSYSRLED
                     KSPAGGAALLSEWKGTGAGKSFCFATANVCLLPDSLARLNNVFNTQARAKEIGQRIRN
                     GAARPQIKIYIDSPTNTSISAASFSSLVSPQGSDGARAVPGSIKRTASVEYKGDGGRH
                     PSDEAANGPASGEQADGSLEDSCIVRIGGEEGGRAQEADDPAPGSQARNGAGGTPKGQ
                     MPNHNQRDGDSGSLGSPSASRESLVKARAGQDSGGSGEPGSNSKLLYKTSVVKKAAAR
                     RRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAAAKLKEQLHGYFEYILYDVGVYGC
                     HGCCNFKCLNSGLFFASRYPVMDVAYHCYPNGCSFDALASKGALFLKVQVGSTPQDQR
                     IVGYIACTHLHAPPEDSAIRCEQLDLLQDWLADFRKSTSSTSTANPEELVVFDVICGD
                     LNFDNCSSDDKLEQQHSLFTRYKDPCRLGPGEEKPWAIGTLLDINGLYDEDVCTPDNL
                     QKVLESEEGRREYLAFPTSKSPGAGQKGRKDLLKGNGRRIDYMLHAEEGLCPDWKAEV
                     EEFSFITQLSGLTDHLPVAMRLMVSAGEEEA"
     exon            1678..1753
                     /gene="Smpd3"
                     /gene_synonym="cca1"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1909
                     /gene="Smpd3"
                     /gene_synonym="cca1"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..1999
                     /gene="Smpd3"
                     /gene_synonym="cca1"
                     /inference="alignment:Splign:2.1.0"
     exon            2000..2063
                     /gene="Smpd3"
                     /gene_synonym="cca1"
                     /inference="alignment:Splign:2.1.0"
     exon            2064..2226
                     /gene="Smpd3"
                     /gene_synonym="cca1"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..5034
                     /gene="Smpd3"
                     /gene_synonym="cca1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggtgctgag tccgagggag gctccggatg ccagagccgc ggctgctgca accctcgccg
       61 ctgtgagaga agcctctggg aagccccggt cgctaggagc tgggaggcgc cgcgtgcgct
      121 gccccgcgcc gagcgccctc gtgagcaccg tctgaggttt tcaacaagct tccatgtccc
      181 agcatggccc cttaaagtga accatgactg tctgtgggac actcaccact gatcatccag
      241 agtcccatct caccaggagg tgacctgtcc tccaccctcc acctgccccc gcctgggaaa
      301 gtgctgcagc tgccacggac accatgtagt agggccggct gcggcgccca gtgagctgca
      361 atggttttgt acacgacccc ctttcctaac agctgtctgt ctgccctcca cgctgtgtcc
      421 tgggccctca tcttcccatg ttactggctg gtggaccggc tggtcgcctc cttcataccc
      481 accacctatg agaagcgcca gagggcagat gacccttgct acctgcagct gttctgcacc
      541 gtactcttca cgccagtcta cctggctctg cttgtggctg cgctgccttt tgcctttctt
      601 gggttcatct tctggtctcc actgcagtct gcccgccggc cctactccta ctcccggcta
      661 gaagacaaga gcccagctgg tggggcagcc ctgcttagtg aatggaaggg cacaggggct
      721 ggcaaaagct tttgctttgc cacagccaat gtctgcctcc tccctgactc gctcgcaagg
      781 ctcaataatg ttttcaacac ccaagcccgg gccaaagaga ttgggcagag aatccgcaat
      841 ggggccgccc ggcctcagat caaaatctac attgattccc ccaccaacac ctccatcagt
      901 gcagccagct tcagcagcct ggtgtcgccg cagggcagtg atggggccag agctgttcct
      961 gggagcatta agaggacagc ctctgtggaa tacaagggag atggtgggcg tcaccccagc
     1021 gatgaggctg ccaatggccc agcctctggg gaacaggctg atggaagcct tgaggacagc
     1081 tgcattgtgc gaattggtgg tgaggaagga ggccgggcac aggaagctga tgaccctgct
     1141 cctgggagcc aagccaggaa tggggctggt gggaccccaa agggccagat gcccaaccac
     1201 aatcagcggg acggggattc tgggagcctg ggcagcccct ccgcctccag ggagtccttg
     1261 gtgaaggcac gggctggaca agacagtggt ggcagcggag agccaggcag taacagtaag
     1321 ctgctgtata agacctctgt ggtgaagaag gcagcagccc gcaggaggcg ccaccctgat
     1381 gaagccttcg accatgaggt ctcagccttc ttcccagcca acctggactt cctgtgcctg
     1441 caggaggtgt ttgacaagcg tgcagctgcc aagttgaaag agcagctaca tggctacttc
     1501 gagtacatcc tgtatgatgt tggggtctat ggctgccatg gttgctgcaa tttcaaatgt
     1561 ctcaacagcg gtctcttctt tgccagccgc taccctgtta tggacgtggc ctatcactgt
     1621 taccccaatg ggtgcagctt cgatgccctg gcctctaagg gagctctgtt tctcaaggtg
     1681 caggtgggaa gcacacctca ggaccaaaga attgttgggt acatcgcctg tacacacctg
     1741 catgccccac cagaggacag tgccatccgg tgtgagcagc tggacctgct tcaggactgg
     1801 ctggctgatt tccgaaaatc tacctcctcg accagcacag ccaaccccga ggagctggtg
     1861 gtgtttgacg tcatctgtgg agatttgaac tttgataact gctcttctga tgacaagctg
     1921 gagcagcagc attcactgtt tactcgctac aaggacccct gccgcctggg gcctggggag
     1981 gaaaagcctt gggccattgg caccctgctg gacataaacg gtctctatga tgaggatgtg
     2041 tgtaccccag acaatcttca aaaggtcttg gagagtgagg aaggccgcag ggagtatctg
     2101 gcctttccca ccagcaagag cccaggggct ggacagaagg gacggaaaga tctgttgaag
     2161 ggcaatggcc gccgcatcga ctacatgctg cacgctgaag aggggctgtg ccctgactgg
     2221 aaggctgagg tggaagaatt cagttttatc acccagctgt ccggcctgac cgaccacctc
     2281 cccgtggcca tgcggctgat ggtgtctgca ggggaagagg aggcgtagac cagccatgcc
     2341 actgatctcg agcagtgggg cctctgccag cccttcaagc cacagcccat gtctggctgt
     2401 gtcccctcca ctgagcacct agtgctgcgg ggaggagggc aggggcaggg aagagcgagc
     2461 ccaccaatcc cctgcaagcc tgcaagccag gctgctctgt gcctctgggc catctctgcg
     2521 gacagaggac ttgggccagc ctgggagccc cagccctctg accagtgcca ttctttccag
     2581 tcacgatttt ctacaaaaat acacagcaca acacagtttg taacccaagg gttcgtatga
     2641 ggaccgggtt tatgttctct ttgtatctct tctcgagctt tgtccagggt tcatcgcttt
     2701 tgtctgctgc caatgttgtc tcgcatgcct gcaccctcgc atgcccgctg cccgcatgcc
     2761 gtgtgccaca ctgtagcacc cacctagact ccttgctcgg gcctcaccta aggccaaact
     2821 caaaacacaa aacaatcaga accagccaaa gaagcacttc ctgggcacag ttgccatctc
     2881 ccctccagct cggtgggggt gtgactgacg gctgtcatta cctgccagcc caggcctctt
     2941 cccagggtct tgcattcaag ggcgattaca ttttaagatg aaaacagaaa acgttaaaca
     3001 caaaatcacc cttcggttag ggtctgtaag tcactgggta gaactgtgct cttccccagc
     3061 ctacacagag ggtccagctg gctctggcca ccccagcagc tcctgtaggg gactgacacc
     3121 agctgtgggg ccctagcagg aaatcccaga gggatcactt ttgtacagga aggggaccca
     3181 gagcatgggg ggagggttca gattttgaag acgacaggtt tgtagcttct tcctacccaa
     3241 tatgtcctct tctatggggc cccttgggtt aacctcttcc tgaggacttc cctcatccat
     3301 gtgtggctga gctcaggaac acttgcctgc cccaagtcag gttgcacctc tggcctccca
     3361 ggcccaccct tcctcagtgg ctaacctgaa tgaagtggca ggaaaccaag atatagagaa
     3421 tggcttcttc accactgaaa gctcaggtgg ccagccaaca gctactcctt gtctagggca
     3481 gggctgattc cagacagcat ccacaagcca ctcttgccac tgtgactaga gccagcccca
     3541 agctcagcac agtcaggctg ctagaagccc actgctctcc acctgtcctg ctcaggtgcc
     3601 caagagcgca ctgactccgc cagcctccct tcagagagca gaggctgatc cctgactaca
     3661 aaaggaagat agtctgtctc aggctgctga gctactgatt tgagggactt ctgatatccc
     3721 tgatggtctc ttcaaagtcc tttgatcttg tatctggcct ggctcaaggc caactctgcc
     3781 ttgtgatgga gggagcatca ggcccctgct gttgggacct ctaaaatgct ggtgccctga
     3841 agctgtgcct tgtcacccat tgagtagcaa gaggaatcca gagaggcaca catttaacag
     3901 atgccaggcc ctgtgctgag tggatccttc agagtatggc agcttggcac tagggcattt
     3961 acagctggtg acactggcct cttaacctct tggcctagag aaccctgccc atgtttgagt
     4021 gtccagagct aggacagcct tgctgggctc tctgctatgc ccctcttctg ccagggcctg
     4081 acctcccagg aatctgggag gtcctcatgg aatgaaaggt ccctactcct ggaaacaacc
     4141 aagattctaa cattcaaggc accattgggc aggcctaacc cctaaaccaa tggcactgtg
     4201 tacaaggaaa gtagatgagg ccaaggccac ggctggggtc cctaaacctg ggttagggta
     4261 aaacgtttgg agtcagagcc gcatccttct ctccttccca actcccaggg cggccttagg
     4321 caagtcaaac agccttgtgc ctctgttatt acaaggtcac ctctacacag tcaggttctg
     4381 aagcccagag gagggacagc ctgccttcag gtcccctaca cttctcagca ctgaggccat
     4441 gaccatccgc cacagttcac tgtggtttgc ctgtggactc agggctcgtg tactgccagg
     4501 cctttctatt tcaactttta aaacagaaga gacattgcca ggattccaga ttccgtgaca
     4561 aggccgacac aggggcagtc acagggacca cttgaatcct aaccggctct gcctgtccag
     4621 gacctctcaa ggaaacatag ataagtgtat tagtttagga aagaccattc agttcatctt
     4681 tatttgtcca aaaccacctg gagttttaag tatgaatatt aaccttgatg ctttttaact
     4741 attgtattaa cttgctcaga tttagaaata ctgttttaaa agaaaacgaa gaaacttttt
     4801 taatttctgt atttttttct gtattgtatc ctcatgggac attaggggtt ttatatggta
     4861 agacacccaa ggttttggta aaacattatc aaatatatat ccagacgatt cttccctaga
     4921 agaaaaaaca atctttatgc ctgattttaa aaagttgaaa agaggtggat ttttccttta
     4981 tggtgctgaa aggaaggatg gagaatgagg agaaaataaa actgtgaggc tcaa
//
