LOCUS       NM_031360               1676 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus sphingomyelin phosphodiesterase 2 (Smpd2), mRNA.
ACCESSION   NM_031360
VERSION     NM_031360.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Silvera,S., Wilkinson,J.A. and LeBlanc,P.J.
  TITLE     Characterization of neutral sphingomyelinase activity and isoform
            expression in rodent skeletal muscle mitochondria
  JOURNAL   Mitochondrion 59, 184-189 (2021)
   PUBMED   34089907
  REMARK    GeneRIF: Characterization of neutral sphingomyelinase activity and
            isoform expression in rodent skeletal muscle mitochondria.
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Tuzcu,H., Unal,B., Kirac,E., Konuk,E., Ozcan,F., Elpek,G.O.,
            Demir,N. and Aslan,M.
  TITLE     Neutral sphingomyelinase inhibition alleviates apoptosis, but not
            ER stress, in liver ischemia-reperfusion injury
  JOURNAL   Free Radic Res 51 (3), 253-268 (2017)
   PUBMED   28277984
  REMARK    GeneRIF: Data herein confirm the role of ceramide in increased
            apoptotic cell death and highlight the protective effect of N-SMase
            inhibition in down-regulation of apoptotic stimuli responses
            occurring in hepatic ischemia reperfusion injury.
REFERENCE   3  (bases 1 to 1676)
  AUTHORS   Bautista-Perez,R., del Valle-Mondragon,L., Cano-Martinez,A.,
            Perez-Mendez,O., Escalante,B. and Franco,M.
  TITLE     Involvement of neutral sphingomyelinase in the angiotensin II
            signaling pathway
  JOURNAL   Am J Physiol Renal Physiol 308 (10), F1178-F1187 (2015)
   PUBMED   25354938
  REMARK    GeneRIF: ANG II stimulates ceramide formation via the activation of
            nSMase in the kidney.
REFERENCE   4  (bases 1 to 1676)
  AUTHORS   Candalija,A., Cubi,R., Ortega,A., Aguilera,J. and Gil,C.
  TITLE     Trk receptors need neutral sphingomyelinase activity to promote
            cell viability
  JOURNAL   FEBS Lett 588 (1), 167-174 (2014)
   PUBMED   24316227
  REMARK    GeneRIF: Data suggest that neurotrophins can promote cell viability
            through Trk receptors in a manner depending on basal neutral
            sphingomyelinase (nSMase) but not through SMase activity
            enhancement.
REFERENCE   5  (bases 1 to 1676)
  AUTHORS   Nusshold,C., Uellen,A., Bernhart,E., Hammer,A., Damm,S.,
            Wintersperger,A., Reicher,H., Hermetter,A., Malle,E. and Sattler,W.
  TITLE     Endocytosis and intracellular processing of BODIPY-sphingomyelin by
            murine CATH.a neurons
  JOURNAL   Biochim Biophys Acta 1831 (12), 1665-1678 (2013)
   PUBMED   23973266
REFERENCE   6  (bases 1 to 1676)
  AUTHORS   Czarny,M., Liu,J., Oh,P. and Schnitzer,J.E.
  TITLE     Transient mechanoactivation of neutral sphingomyelinase in caveolae
            to generate ceramide
  JOURNAL   J Biol Chem 278 (7), 4424-4430 (2003)
   PUBMED   12473648
  REMARK    GeneRIF: a novel physiological function as a new mechanosensor
            initiating the ERK cascade
REFERENCE   7  (bases 1 to 1676)
  AUTHORS   Mizutani,Y., Tamiya-Koizumi,K., Irie,F., Hirabayashi,Y., Miwa,M.
            and Yoshida,S.
  TITLE     Cloning and expression of rat neutral sphingomyelinase:
            enzymological characterization and identification of essential
            histidine residues
  JOURNAL   Biochim Biophys Acta 1485 (2-3), 236-246 (2000)
   PUBMED   10832103
REFERENCE   8  (bases 1 to 1676)
  AUTHORS   Van Sluijters,D.A., Van Woerkom,G.M., Aerts,J.M. and Meijer,A.J.
  TITLE     Sphingomyelinase treatment of rat hepatocytes inhibits
            cell-swelling-stimulated glycogen synthesis by causing cell
            shrinkage
  JOURNAL   Eur J Biochem 266 (2), 653-659 (1999)
   PUBMED   10561609
  REMARK    GeneRIF: sphingomyelinase causes an increase in intracellular
            chloride, which is known to inhibit glycogen synthase phosphatase
REFERENCE   9  (bases 1 to 1676)
  AUTHORS   Dobrowsky,R.T., Jenkins,G.M. and Hannun,Y.A.
  TITLE     Neurotrophins induce sphingomyelin hydrolysis. Modulation by
            co-expression of p75NTR with Trk receptors
  JOURNAL   J Biol Chem 270 (38), 22135-22142 (1995)
   PUBMED   7673191
REFERENCE   10 (bases 1 to 1676)
  AUTHORS   Dobrowsky,R.T., Werner,M.H., Castellino,A.M., Chao,M.V. and
            Hannun,Y.A.
  TITLE     Activation of the sphingomyelin cycle through the low-affinity
            neurotrophin receptor
  JOURNAL   Science 265 (5178), 1596-1599 (1994)
   PUBMED   8079174
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC091139.1.
            
            On Mar 27, 2005 this sequence version replaced NM_031360.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC091139.1, AB047002.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20q12"
     gene            1..1676
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /note="sphingomyelin phosphodiesterase 2"
                     /db_xref="GeneID:83537"
                     /db_xref="RGD:619753"
     exon            1..311
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /inference="alignment:Splign:2.1.0"
     CDS             262..1530
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /EC_number="3.1.4.12"
                     /note="N-SMase; lyso-PAF-PLC; lyso-platelet-activating
                     factor-phospholipase C; neutral sphingomyelinase;
                     sphingomyelin phosphodiesterase 2, neutral membrane"
                     /codon_start=1
                     /product="sphingomyelin phosphodiesterase 2"
                     /protein_id="NP_112650.1"
                     /db_xref="GeneID:83537"
                     /db_xref="RGD:619753"
                     /translation="MKHNFSLRLRVFNLNCWDIPYLSKHRADRMKRLGDFLNLESFDL
                     ALLEEVWSEQDFQYLKQKLSLTYPDAHYFRSGIIGSGLCVFSRHPIQEIVQHVYTLNG
                     YPYKFYHGDWFCGKAVGLLVLHLSGLVLNAYVTHLHAEYSRQKDIYFAHRVAQAWELA
                     QFIHHTSKKANVVLLCGDLNMHPKDLGCCLLKEWTGLRDAFVETEDFKGSEDGCTMVP
                     KNCYVSQQDLGPFPFGVRIDYVLYKAVSGFHICCKTLKTTTGCDPHNGTPFSDHEALM
                     ATLCVKHSPPQEDPCSAHGSAERSALISALREARTELGRGIAQARWWAALFGYVMILG
                     LSLLVLLCVLAAGEEAREVAIMLWTPSVGLVLGAGAVYLFHKQEAKSLCRAQAEIQHV
                     LTRTTETQDLGSEPHPTHCRQQEADRAEEK"
     exon            312..408
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /inference="alignment:Splign:2.1.0"
     exon            409..485
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /inference="alignment:Splign:2.1.0"
     exon            486..579
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /inference="alignment:Splign:2.1.0"
     exon            580..669
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /inference="alignment:Splign:2.1.0"
     exon            670..752
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /inference="alignment:Splign:2.1.0"
     exon            753..885
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /inference="alignment:Splign:2.1.0"
     exon            886..990
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1144
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1551
                     /gene="Smpd2"
                     /gene_synonym="nSMase"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caccgaaggc tctaagaacc tggaacggtc cacccgagac tccttccggg actgccggcg
       61 gcctcgagca gccagcccag cactagcggc cgatctcctt tcaggtggga ggacggcctg
      121 ttgtcccagt aacgcaggag tcgtcccccc ccccccccgc cccaaaccag ctcgcgttcc
      181 tgggtcgggg cagcgcggga cagcgccggc aacctgtgcg cgcggtcagc ctcgccaccc
      241 ttgctccgaa gcactccaac catgaagcac aacttttctc tgcggctgag ggttttcaac
      301 ctcaactgct gggacatccc ctacctaagc aagcataggg ccgaccgcat gaagcgcttg
      361 ggagactttc tgaacttgga gagctttgac ctggctctgc tggaggaggt gtggagtgag
      421 caggacttcc agtacttaaa gcaaaagcta tcgctcacct acccggatgc acactacttc
      481 agaagcggaa tcattggcag tggcctctgt gtgttctcca gacacccaat ccaggaaatt
      541 gtccagcacg tctatactct caatggttac ccctacaagt tctatcatgg agactggttc
      601 tgtgggaagg ctgtggggct gctggtgctc catctaagcg gactggtgct caacgcctac
      661 gtgacccatc tgcacgctga gtacagccgg cagaaggata tctacttcgc acaccgtgtg
      721 gcccaagctt gggaactggc ccagttcatc caccacacat ccaagaaagc aaatgtggtc
      781 ctgttgtgcg gggatctcaa catgcacccc aaagacctgg gctgctgcct actgaaagag
      841 tggacggggc tccgtgatgc ctttgttgag accgaggact ttaagggctc agaggacggc
      901 tgtaccatgg tacccaagaa ctgctacgtc agccagcagg acctggggcc gtttcccttt
      961 ggcgtccgta tcgactacgt gctttacaag gcagtctctg gcttccacat ctgctgtaag
     1021 actctgaaaa ccactacggg ctgtgacccc cacaatggca cccccttctc tgaccacgag
     1081 gccctcatgg ctactttgtg tgtgaagcac agcccccctc aggaagaccc ttgctctgcc
     1141 catggatcag cggaaaggtc agcattgatc agcgcgctaa gggaggcgag gacagagctg
     1201 gggcgaggca tagctcaagc tcgctggtgg gctgcactct ttggctatgt gatgattttg
     1261 gggctgtccc tcctggtgtt gctgtgtgtc ctggctgcag gagaagaggc cagggaagtg
     1321 gccataatgc tctggacacc cagtgtgggt ctggtgctgg gggcaggcgc agtctacctg
     1381 ttccacaagc aggaggccaa aagcttatgt cgggcccagg ctgagataca acatgttctg
     1441 acaaggacaa cggagaccca ggacctaggc tcagagcctc acccaaccca ctgccggcag
     1501 caggaggcgg acagagctga agaaaaataa aacttgcttg acacactcta aaaaaaaaaa
     1561 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1621 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa
//
