LOCUS       NM_031536                867 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Rattus norvegicus sperm mitochondria-associated cysteine-rich
            protein (Smcp), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_031536
VERSION     NM_031536.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Maiorino,M., Roveri,A., Benazzi,L., Bosello,V., Mauri,P., Toppo,S.,
            Tosatto,S.C. and Ursini,F.
  TITLE     Functional interaction of phospholipid hydroperoxide glutathione
            peroxidase with sperm mitochondrion-associated cysteine-rich
            protein discloses the adjacent cysteine motif as a new substrate of
            the selenoperoxidase
  JOURNAL   J Biol Chem 280 (46), 38395-38402 (2005)
   PUBMED   16159880
  REMARK    GeneRIF: PHGPx interacts with SMCP; the cysteine motif of PHGPx is
            a new substrate of the selenoperoxidase
REFERENCE   2  (bases 1 to 867)
  AUTHORS   Nayernia,K., Drabent,B., Meinhardt,A., Adham,I.M., Schwandt,I.,
            Muller,C., Sancken,U., Kleene,K.C. and Engel,W.
  TITLE     Triple knockouts reveal gene interactions affecting fertility of
            male mice
  JOURNAL   Mol Reprod Dev 70 (4), 406-416 (2005)
   PUBMED   15685642
REFERENCE   3  (bases 1 to 867)
  AUTHORS   Kwitek,A.E., Gullings-Handley,J., Yu,J., Carlos,D.C., Orlebeke,K.,
            Nie,J., Eckert,J., Lemke,A., Andrae,J.W., Bromberg,S., Pasko,D.,
            Chen,D., Scheetz,T.E., Casavant,T.L., Soares,M.B., Sheffield,V.C.,
            Tonellato,P.J. and Jacob,H.J.
  TITLE     High-density rat radiation hybrid maps containing over 24,000
            SSLPs, genes, and ESTs provide a direct link to the rat genome
            sequence
  JOURNAL   Genome Res 14 (4), 750-757 (2004)
   PUBMED   15060019
REFERENCE   4  (bases 1 to 867)
  AUTHORS   Nayernia,K., Meinhardt,A., Drabent,B., Adham,I.M., Muller,C.,
            Steckel,M., Sancken,U. and Engel,W.
  TITLE     Synergistic effects of germ cell expressed genes on male fertility
            in mice
  JOURNAL   Cytogenet Genome Res 103 (3-4), 314-320 (2003)
   PUBMED   15051954
REFERENCE   5  (bases 1 to 867)
  AUTHORS   Nayernia,K., Adham,I.M., Burkhardt-Gottges,E., Neesen,J.,
            Rieche,M., Wolf,S., Sancken,U., Kleene,K. and Engel,W.
  TITLE     Asthenozoospermia in mice with targeted deletion of the sperm
            mitochondrion-associated cysteine-rich protein (Smcp) gene
  JOURNAL   Mol Cell Biol 22 (9), 3046-3052 (2002)
   PUBMED   11940662
REFERENCE   6  (bases 1 to 867)
  AUTHORS   Cataldo,L., Baig,K., Oko,R., Mastrangelo,M.A. and Kleene,K.C.
  TITLE     Developmental expression, intracellular localization, and selenium
            content of the cysteine-rich protein associated with the
            mitochondrial capsules of mouse sperm
  JOURNAL   Mol Reprod Dev 45 (3), 320-331 (1996)
   PUBMED   8916043
REFERENCE   7  (bases 1 to 867)
  AUTHORS   Adham,I.M., Tessmann,D., Soliman,K.A., Murphy,D., Kremling,H.,
            Szpirer,C. and Engel,W.
  TITLE     Cloning, expression, and chromosomal localization of the rat
            mitochondrial capsule selenoprotein gene (MCS): the reading frame
            does not contain potential UGA selenocysteine codons
  JOURNAL   DNA Cell Biol 15 (2), 159-166 (1996)
   PUBMED   8634143
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            U48702.1 and BF415595.1.
            
            On Aug 4, 2006 this sequence version replaced NM_031536.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U48702.1, X87883.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-855               U48702.1           7-861
            856-867             BF415595.1         1-12                c
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     gene            1..867
                     /gene="Smcp"
                     /gene_synonym="Mcs; Mcsp"
                     /note="sperm mitochondria-associated cysteine-rich
                     protein"
                     /db_xref="GeneID:24899"
                     /db_xref="RGD:3069"
     exon            1..154
                     /gene="Smcp"
                     /gene_synonym="Mcs; Mcsp"
                     /inference="alignment:Splign:2.1.0"
     exon            155..857
                     /gene="Smcp"
                     /gene_synonym="Mcs; Mcsp"
                     /inference="alignment:Splign:2.1.0"
     CDS             175..612
                     /gene="Smcp"
                     /gene_synonym="Mcs; Mcsp"
                     /codon_start=1
                     /product="sperm mitochondrial-associated cysteine-rich
                     protein"
                     /protein_id="NP_113724.1"
                     /db_xref="GeneID:24899"
                     /db_xref="RGD:3069"
                     /translation="MSDSSKTNQCCPTPCCPPKPCCPPKPCCLQKSPCCPKSPCCPPK
                     SPCCTPKVCPCPTPCPCPATCPAACACPCPMKPCCPTKCTCCPKKCTCCPQPTCCVQP
                     TCCSSENKTESDSDGSGQTQDRGAQTQQSPQGGQGNWNQKKTK"
ORIGIN      
        1 tttggcttct gatagtcatg gactcactag actgctgagg aagatccagc atctatctgc
       61 tgaaaacatc cagaaaacta cttttaacac cagaatcaag catggaagtg gtgaactaag
      121 aagagcctga ggaagaattg tgttgcctga tcagaaactc caactctaaa gaagatgagt
      181 gactcatcca aaaccaacca gtgctgcccc actccatgct gcccaccaaa accatgttgc
      241 ccacccaaac cgtgctgtct gcagaaatca ccttgctgtc ccaagtcccc atgctgtcca
      301 cccaaatccc catgttgcac accaaaggtc tgtccctgtc cgactccctg tccctgtcca
      361 gccacctgtc cagctgcctg tgcttgtcct tgtccgatga aaccatgctg cccaacgaag
      421 tgtacatgct gccctaaaaa gtgcacctgc tgcccacagc ccacctgctg tgttcaaccc
      481 acctgctgct cttctgaaaa caagactgag tcagattctg atgggtctgg tcaaactcag
      541 gataggggtg ctcaaacaca gcagtcccct caaggtggtc aaggcaattg gaaccagaag
      601 aagaccaagt aaactgtccc tgacaccatg ctcatcctca aatggtgtgg gggattacta
      661 caggcaaggt taagactatg ttgtagcgat gctgttttca tgataaccaa caagaccact
      721 caaccataag ctaccatttc gacctaacca taggctacta ttgcaactgg aaatggaaag
      781 tagaaaagga tagaaacatc ttgtctagtg atcctgacat ttagatagca aagaaataaa
      841 agagcaaata aaaagaaaaa aaaaaaa
//
