LOCUS       NM_019275               3056 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus SMAD family member 4 (Smad4), mRNA.
ACCESSION   NM_019275
VERSION     NM_019275.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Wang,Z., Chen,Q., Yang,Z. and Long,R.
  TITLE     MiR-125b aggregates osteoporosis in postmenopausal rats via
            targeting Smad4
  JOURNAL   Minerva Surg 77 (5), 511-512 (2022)
   PUBMED   34889563
  REMARK    GeneRIF: MiR-125b aggregates osteoporosis in postmenopausal rats
            via targeting Smad4.
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Shojaei-Zarghani,S., Yari Khosroushahi,A. and Rafraf,M.
  TITLE     Oncopreventive effects of theanine and theobromine on
            dimethylhydrazine-induced colon cancer model
  JOURNAL   Biomed Pharmacother 134, 111140 (2021)
   PUBMED   33360052
REFERENCE   3  (bases 1 to 3056)
  AUTHORS   Wong,C.K., Lambert,A.W., Ozturk,S., Papageorgis,P., Lopez,D.,
            Shen,N., Sen,Z., Abdolmaleky,H.M., Gyorffy,B., Feng,H. and
            Thiagalingam,S.
  TITLE     Targeting RICTOR Sensitizes SMAD4-Negative Colon Cancer to
            Irinotecan
  JOURNAL   Mol Cancer Res 18 (3), 414-423 (2020)
   PUBMED   31932471
REFERENCE   4  (bases 1 to 3056)
  AUTHORS   Aragon,E., Wang,Q., Zou,Y., Morgani,S.M., Ruiz,L., Kaczmarska,Z.,
            Su,J., Torner,C., Tian,L., Hu,J., Shu,W., Agrawal,S., Gomes,T.,
            Marquez,J.A., Hadjantonakis,A.K., Macias,M.J. and Massague,J.
  TITLE     Structural basis for distinct roles of SMAD2 and SMAD3 in FOXH1
            pioneer-directed TGF-beta signaling
  JOURNAL   Genes Dev 33 (21-22), 1506-1524 (2019)
   PUBMED   31582430
REFERENCE   5  (bases 1 to 3056)
  AUTHORS   Ni,J., Wu,Q.Q., Liao,H.H., Fan,D. and Tang,Q.Z.
  TITLE     Bcl6 Suppresses Cardiac Fibroblast Activation and Function via
            Directly Binding to Smad4
  JOURNAL   Curr Med Sci 39 (4), 534-540 (2019)
   PUBMED   31346987
  REMARK    GeneRIF: The results showed that Bcl6 overexpression induced
            decreased proliferation and reduced activation of fibroblasts which
            were stimulated with TGFb1. It was found that activated smad2 and
            smad3 were not changed by overexpressing Bcl6, but smad4 was
            decreased.
REFERENCE   6  (bases 1 to 3056)
  AUTHORS   Chen,X., Weisberg,E., Fridmacher,V., Watanabe,M., Naco,G. and
            Whitman,M.
  TITLE     Smad4 and FAST-1 in the assembly of activin-responsive factor
  JOURNAL   Nature 389 (6646), 85-89 (1997)
   PUBMED   9288972
REFERENCE   7  (bases 1 to 3056)
  AUTHORS   Nakao,A., Imamura,T., Souchelnytskyi,S., Kawabata,M., Ishisaki,A.,
            Oeda,E., Tamaki,K., Hanai,J., Heldin,C.H., Miyazono,K. and ten
            Dijke,P.
  TITLE     TGF-beta receptor-mediated signalling through Smad2, Smad3 and
            Smad4
  JOURNAL   EMBO J 16 (17), 5353-5362 (1997)
   PUBMED   9311995
REFERENCE   8  (bases 1 to 3056)
  AUTHORS   Topper,J.N., Cai,J., Qiu,Y., Anderson,K.R., Xu,Y.Y., Deeds,J.D.,
            Feeley,R., Gimeno,C.J., Woolf,E.A., Tayber,O., Mays,G.G.,
            Sampson,B.A., Schoen,F.J., Gimbrone,M.A. Jr. and Falb,D.
  TITLE     Vascular MADs: two novel MAD-related genes selectively inducible by
            flow in human vascular endothelium
  JOURNAL   Proc Natl Acad Sci U S A 94 (17), 9314-9319 (1997)
   PUBMED   9256479
REFERENCE   9  (bases 1 to 3056)
  AUTHORS   Wu,R.Y., Zhang,Y., Feng,X.H. and Derynck,R.
  TITLE     Heteromeric and homomeric interactions correlate with signaling
            activity and functional cooperativity of Smad3 and Smad4/DPC4
  JOURNAL   Mol Cell Biol 17 (5), 2521-2528 (1997)
   PUBMED   9111321
REFERENCE   10 (bases 1 to 3056)
  AUTHORS   Zhang,Y., Feng,X., We,R. and Derynck,R.
  TITLE     Receptor-associated Mad homologues synergize as effectors of the
            TGF-beta response
  JOURNAL   Nature 383 (6596), 168-172 (1996)
   PUBMED   8774881
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000018.1 and AB010954.1.
            
            On Apr 28, 2015 this sequence version replaced NM_019275.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF056002.1, AB010954.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760393,
                                           SAMEA5760400 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-377               JAXUCZ010000018.1  69549308-69549684   c
            378-552             JAXUCZ010000018.1  69547453-69547627   c
            553-582             JAXUCZ010000018.1  69546816-69546845   c
            583-795             JAXUCZ010000018.1  69539808-69540020   c
            796-915             JAXUCZ010000018.1  69535966-69536085   c
            916-1032            JAXUCZ010000018.1  69535758-69535874   c
            1033-1083           JAXUCZ010000018.1  69534436-69534486   c
            1084-1267           JAXUCZ010000018.1  69527147-69527330   c
            1268-1436           JAXUCZ010000018.1  69526312-69526480   c
            1437-1575           JAXUCZ010000018.1  69521724-69521862   c
            1576-1914           AB010954.1         1504-1842
            1915-3056           JAXUCZ010000018.1  69518988-69520129   c
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18q12.2"
     gene            1..3056
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /note="SMAD family member 4"
                     /db_xref="GeneID:50554"
                     /db_xref="RGD:3033"
     exon            1..377
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /inference="alignment:Splign:2.1.0"
     CDS             129..1787
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /note="SMAD 4; mothers against DPP homolog 4; MAD homolog
                     4; MAD (mothers against decapentaplegic Drosophila)
                     homolog 4"
                     /codon_start=1
                     /product="mothers against decapentaplegic homolog 4"
                     /protein_id="NP_062148.1"
                     /db_xref="GeneID:50554"
                     /db_xref="RGD:3033"
                     /translation="MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLV
                     KKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWP
                     DLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPPSMLVKD
                     EYVHDFEGQPSLPTEGHSIQTIQHPPSNRASTETYSAPALLAPSESNATSTTNFPNIP
                     VASTSQPASILAGSHSEGLLQIASGPQPGQQQNGFTAQPATYHHNSTTTWTGSRTAPY
                     TPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQ
                     VGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECK
                     GEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQ
                     AATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKG
                     WGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD"
     exon            378..552
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /inference="alignment:Splign:2.1.0"
     exon            553..582
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /inference="alignment:Splign:2.1.0"
     exon            583..795
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /inference="alignment:Splign:2.1.0"
     exon            796..915
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1032
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1083
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1267
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1436
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1575
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..3056
                     /gene="Smad4"
                     /gene_synonym="Madh4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 attatcctgc tcaccagatg tcttgaaaat ttttcttaca acattggcca ttggttttca
       61 ctgccttcaa aagatcaaaa ttactccaga aatttgaaag tttgatttaa aagaaaaaac
      121 gtgaacagat ggacaatatg tctattacaa atacaccgac aagcaatgac gcctgtctga
      181 gcattgtaca cagtttgatg tgccacagac aaggtggaga gagtgagaca tttgccaaaa
      241 gagcaattga gagtctggta aagaagctga aagagaagaa agatgaactg gattctttaa
      301 taacagctat cactacaaat ggagctcacc ccagcaagtg tgtcaccata cagagaacac
      361 tggatggacg actgcaggtg gctggtcgga aaggatttcc tcatgtgatc tatgcccgtc
      421 tgtggaggtg gcctgatcta cacaagaacg aactaaaaca tgttaaatat tgtcagtatg
      481 catttgactt aaaatgtgat agcgtctgtg tgaacccata tcactatgag cgggttgtct
      541 cacctggaat tgatctctct ggattaacac tgcagagtaa tgctccacca agtatgttag
      601 tgaaggatga atatgttcat gactttgaag gacagccatc cttacccacg gaaggacatt
      661 cgattcaaac catccaacat ccaccaagta atcgcgcatc cacggagacg tacagcgccc
      721 cagctctgtt agccccatca gagtctaatg ccaccagtac caccaacttc cccaacattc
      781 ctgtggcttc cacaagtcag ccggccagca ttctggcagg cagccatagt gaaggactgt
      841 tgcagatagc ttcagggcct cagccaggac agcagcagaa tggattcact gctcagccag
      901 ctacttacca ccataacagc actaccacct ggactggaag taggactgca ccatacacac
      961 ctaatttgcc tcaccaccaa aacggccatc ttcagcacca cccgcctatg ccgccccatc
     1021 ctggacatta ctggccggtt cacaatgagc ttgcattcca gcctcccatt tccaatcatc
     1081 ctgctcctga atattggtgt tccattgctt actttgaaat ggatgttcag gtaggagaga
     1141 catttaaagt cccttcaagc tgtcctattg taactgtgga tggatacgtg gatccttctg
     1201 gaggagatcg cttttgcttg ggtcaactct ccaatgtcca caggacagaa gctattgaga
     1261 gagcaaggtt gcacataggc aaaggagtgc agttggagtg taaaggtgaa ggggacgtgt
     1321 gggtcaggtg cctcagtgac cacgccgtct tcgtgcagag ttactacctg gaccgtgaag
     1381 ctggccgagc acctggtgac gccgtccaca agatctaccc gagtgcgtat ataaaggtct
     1441 ttgatttgcg gcagtgtcac cggcagatgc agcagcaggc ggccaccgcg caagccgcag
     1501 ctgctgccca ggcggcagct gtggcaggaa acatccctgg tcctgggtcc gtaggtggaa
     1561 tagccccggc catcagtctg tctgctgctg ctggcattgg tgtagacgac ctccggcgct
     1621 tgtgcatact caggatgagc ttcgtgaagg gctggggccc agactacccg agacagagca
     1681 tcaaagaaac cccgtgctgg attgagattc acctccaccg agctctgcag ctcctggatg
     1741 aagtcctgca caccatgccc attgcggacc cacagccttt agactgagat ctcacaccat
     1801 ggatgcccta accatttcca ggatggtgga ctatgaaata tactcgtgtt tataatctga
     1861 agatttattg catttttgtt ctgctctgtc ttttcataaa gggttgaaag atgtgtttgc
     1921 tgccttgctc ttagcagaca gaaactgaat taaaactgtt tttctatttt agaactttca
     1981 ggcatggctc agagctcgaa gatcaggaag atccaatttt tactaagtct tcactggttg
     2041 ttatgtatga aggagtcatt ccagtgctgg gaactcagcc ctctaagtat gcgagagaga
     2101 gctccttaca tgcaggacac tgatgtgttg tctgcagaat aaattcactg ttgctgactt
     2161 taaggctgag aagttctcaa agttaagtca cctgttactt agtgggcaaa gtcattgctg
     2221 aacgagctgc acgcaagtgg cagtccagtc ttccaaaagc agcttcactc ccttaaagcc
     2281 ttttgtcagt ctttattcag aatggtgttt gcaagttaaa cagtgaaggt gaattaaatt
     2341 catagtgtct tgcagacttt agggtttttc cccaagacaa aacactaatc tgtgtgcata
     2401 ttgacaattc cttacaatta ccagtcaaag aaatgccatt taaaattaca gttttatccc
     2461 taatggatgg ccatccccga gagatacact gtgccctgtt aaacagtaca tgaattcttc
     2521 tatatttcta ggtgcaaggt tgcttataaa aaacaaagcc taggggagga attttttttc
     2581 ccttaattca tagggagaag gttttgtatt tataaaaaca ctaaaagcag agttgctctg
     2641 cctgatgctt cactgttctg caaggtggca gtgcttcaac tgaaataatg actattttgg
     2701 aaactgctaa attctatgtt aaagactgtg cagaataacg gaaaccgtgc agttggtaac
     2761 aggtagtttg gatatttttg tacttgattt gatgtgtgac tttttttcat atactgttta
     2821 aatcatgtat gttttgacat tgtttaaaat tcagtttttg tatcttgggg caagactgca
     2881 aactttttta taccttttgg ttatcctaag cccttcgttt gccatcattg atcccttggt
     2941 ggtgactttg tagacgttga agtagaagcg tgggacgcat tgactgtacc acagacatgt
     3001 atacttttta cctagttact agagtgaata aaactgagtc catatacgaa gttgaa
//
