LOCUS       NM_013095               5037 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Rattus norvegicus SMAD family member 3 (Smad3), mRNA.
ACCESSION   NM_013095
VERSION     NM_013095.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Hu,H.J., Wang,X.H., Zhang,Z.Z., Ou,Y., Ning,Z.H., Yang,J.Y.,
            Huang,H., Tang,H.F. and Jiang,Z.S.
  TITLE     SIRT3 sulfhydration using hydrogen sulfide inhibited angiotensin
            II-induced atrial fibrosis and vulnerability to atrial fibrillation
            via suppression of the TGF-beta1/smad2/3 signalling pathway
  JOURNAL   Eur J Pharmacol 982, 176900 (2024)
   PUBMED   39168432
  REMARK    GeneRIF: SIRT3 sulfhydration using hydrogen sulfide inhibited
            angiotensin II-induced atrial fibrosis and vulnerability to atrial
            fibrillation via suppression of the TGF-beta1/smad2/3 signalling
            pathway.
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Kim,S.H., Oh,J.M., Roh,H., Lee,K.W., Lee,J.H. and Lee,W.J.
  TITLE     Zinc-Alpha-2-Glycoprotein Peptide Downregulates Type I and III
            Collagen Expression via Suppression of TGF-beta and p-Smad 2/3
            Pathway in Keloid Fibroblasts and Rat Incisional Model
  JOURNAL   Tissue Eng Regen Med 21 (7), 1079-1092 (2024)
   PUBMED   39105875
  REMARK    GeneRIF: Zinc-Alpha-2-Glycoprotein Peptide Downregulates Type I and
            III Collagen Expression via Suppression of TGF-beta and p-Smad 2/3
            Pathway in Keloid Fibroblasts and Rat Incisional Model.
REFERENCE   3  (bases 1 to 5037)
  AUTHORS   Xia,Y., Wu,Y., Gong,Y., Yue,C., Tao,L., Xin,T., Shen,C., Zhu,Y.,
            Shen,M. and Shen,J.
  TITLE     Brachyury promotes extracellular matrix synthesis through
            transcriptional regulation of Smad3 in nucleus pulposus
  JOURNAL   Am J Physiol Cell Physiol 326 (5), C1384-C1397 (2024)
   PUBMED   38690917
  REMARK    GeneRIF: Brachyury promotes extracellular matrix synthesis through
            transcriptional regulation of Smad3 in nucleus pulposus.
REFERENCE   4  (bases 1 to 5037)
  AUTHORS   Qiu,W., Sun,Q., Li,N., Chen,Z., Wu,H., Chen,Z., Guo,X. and Fang,F.
  TITLE     Superoxide dismutase 2 scavenges ROS to promote osteogenic
            differentiation of human periodontal ligament stem cells by
            regulating Smad3 in alveolar bone-defective rats
  JOURNAL   J Periodontol 95 (5), 469-482 (2024)
   PUBMED   37921754
  REMARK    GeneRIF: Superoxide dismutase 2 scavenges ROS to promote osteogenic
            differentiation of human periodontal ligament stem cells by
            regulating Smad3 in alveolar bone-defective rats.
REFERENCE   5  (bases 1 to 5037)
  AUTHORS   He,C., Gu,L., Li,A., Li,Y., Xiao,R., Liao,J., Mu,J., Gan,Y.,
            Peng,M., Mohan,G., Liu,W., Xu,L. and Guo,S.
  TITLE     Recombinant Slit2 attenuates tracheal fibroblast activation in
            benign central airway obstruction by inhibiting the TGF-beta1/Smad3
            signaling pathway
  JOURNAL   Mol Cell Probes 73, 101947 (2024)
   PUBMED   38122948
  REMARK    GeneRIF: Recombinant Slit2 attenuates tracheal fibroblast
            activation in benign central airway obstruction by inhibiting the
            TGF-beta1/Smad3 signaling pathway.
REFERENCE   6  (bases 1 to 5037)
  AUTHORS   Janknecht,R., Wells,N.J. and Hunter,T.
  TITLE     TGF-beta-stimulated cooperation of smad proteins with the
            coactivators CBP/p300
  JOURNAL   Genes Dev 12 (14), 2114-2119 (1998)
   PUBMED   9679056
REFERENCE   7  (bases 1 to 5037)
  AUTHORS   Nakao,A., Imamura,T., Souchelnytskyi,S., Kawabata,M., Ishisaki,A.,
            Oeda,E., Tamaki,K., Hanai,J., Heldin,C.H., Miyazono,K. and ten
            Dijke,P.
  TITLE     TGF-beta receptor-mediated signalling through Smad2, Smad3 and
            Smad4
  JOURNAL   EMBO J 16 (17), 5353-5362 (1997)
   PUBMED   9311995
REFERENCE   8  (bases 1 to 5037)
  AUTHORS   Wu,R.Y., Zhang,Y., Feng,X.H. and Derynck,R.
  TITLE     Heteromeric and homomeric interactions correlate with signaling
            activity and functional cooperativity of Smad3 and Smad4/DPC4
  JOURNAL   Mol Cell Biol 17 (5), 2521-2528 (1997)
   PUBMED   9111321
REFERENCE   9  (bases 1 to 5037)
  AUTHORS   Chen,Y., Lebrun,J.J. and Vale,W.
  TITLE     Regulation of transforming growth factor beta- and activin-induced
            transcription by mammalian Mad proteins
  JOURNAL   Proc Natl Acad Sci U S A 93 (23), 12992-12997 (1996)
   PUBMED   8917532
REFERENCE   10 (bases 1 to 5037)
  AUTHORS   Zhang,Y., Feng,X., We,R. and Derynck,R.
  TITLE     Receptor-associated Mad homologues synergize as effectors of the
            TGF-beta response
  JOURNAL   Nature 383 (6596), 168-172 (1996)
   PUBMED   8774881
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC064437.1 and JAXUCZ010000008.1.
            
            On Mar 19, 2013 this sequence version replaced NM_013095.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643289.23211.1,
                                           SRR26360194.2362245.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760475 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3145              BC064437.1         1-3145
            3146-5037           JAXUCZ010000008.1  73022204-73024095   c
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q24"
     gene            1..5037
                     /gene="Smad3"
                     /gene_synonym="mad3; Madh3; Smad 3"
                     /note="SMAD family member 3"
                     /db_xref="GeneID:25631"
                     /db_xref="RGD:3032"
     exon            1..408
                     /gene="Smad3"
                     /gene_synonym="mad3; Madh3; Smad 3"
                     /inference="alignment:Splign:2.1.0"
     CDS             203..1480
                     /gene="Smad3"
                     /gene_synonym="mad3; Madh3; Smad 3"
                     /note="mothers against DPP homolog 3; MAD (mothers against
                     decapentaplegic, Drosophila) homolog 3; MAD homolog 3"
                     /codon_start=1
                     /product="mothers against decapentaplegic homolog 3"
                     /protein_id="NP_037227.1"
                     /db_xref="GeneID:25631"
                     /db_xref="RGD:3032"
                     /translation="MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKK
                     TGQLDELEKAITTQNVNTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHE
                     LRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSI
                     PENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNN
                     LDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSN
                     VNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKI
                     PPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTST
                     PCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS"
     exon            409..602
                     /gene="Smad3"
                     /gene_synonym="mad3; Madh3; Smad 3"
                     /inference="alignment:Splign:2.1.0"
     exon            603..734
                     /gene="Smad3"
                     /gene_synonym="mad3; Madh3; Smad 3"
                     /inference="alignment:Splign:2.1.0"
     exon            735..809
                     /gene="Smad3"
                     /gene_synonym="mad3; Madh3; Smad 3"
                     /inference="alignment:Splign:2.1.0"
     exon            810..860
                     /gene="Smad3"
                     /gene_synonym="mad3; Madh3; Smad 3"
                     /inference="alignment:Splign:2.1.0"
     exon            861..1073
                     /gene="Smad3"
                     /gene_synonym="mad3; Madh3; Smad 3"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1211
                     /gene="Smad3"
                     /gene_synonym="mad3; Madh3; Smad 3"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1356
                     /gene="Smad3"
                     /gene_synonym="mad3; Madh3; Smad 3"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..5037
                     /gene="Smad3"
                     /gene_synonym="mad3; Madh3; Smad 3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgcgtggag ccgctcgggc gccgccgtgt tccggatccc cgcaactgca gtgccgctat
       61 cctggccggg acgcctgggc aagttctcca gagttaaaag cgaagttcgg gcgcggcgca
      121 ggccgagctg cctctgtgcg cccccggcgt ccccagtgcg tccagccccg ccgggggcgc
      181 cggtgaccct tccgtgccag ccatgtcgtc catcctgccc ttcacccccc cgatcgtgaa
      241 gcgcctgctg ggctggaaga agggcgagca gaacgggcag gaggagaagt ggtgcgagaa
      301 ggcggtcaag agcttggtga agaagctcaa gaagacgggg cagttggacg agctggagaa
      361 ggccatcacc acgcagaacg tgaacaccaa gtgcattacc atccccaggt cactggatgg
      421 ccggctgcag gtatcccatc ggaaggggct cccccatgtc atctactgcc gcttgtggcg
      481 gtggcccgac ctgcacagcc accatgagtt acgggccatg gagctctgtg agttcgcctt
      541 caacatgaag aaggatgaag tgtgtgtaaa tccttaccac tatcagagag tagagacacc
      601 agtgctacct ccagtgttgg tgccacgcca caccgagatc ccggccgagt tcccccctct
      661 ggatgactac agccattcca tccccgagaa cactaacttc cccgctggca ttgaacccca
      721 gagcaatatt ccagaaaccc cacctcctgg ctacctgagt gaagatggag aaactagcga
      781 ccaccagatg aaccacagca tggacgcagg ctctccaaac ctctccccga atccgatgtc
      841 cccagcacac aataacttgg acctacagcc agtcacctac tgtgagccgg ccttttggtg
      901 ctccatctcc tactacgagc tgaaccagcg agttggggag acattccacg cttcacagcc
      961 gtccatgaca gtagatggct tcactgatcc ctccaattca gagcgcttct gtctgggcct
     1021 gctgtccaat gttaaccgga atgcagccgt ggagcttaca aggcggcaca ttgggagggg
     1081 tgtgcggctc tactacattg gaggggaggt ctttgcagag tgcctcagtg acagtgctat
     1141 tttcgtccag tctcccaact gcaaccagcg ctatggctgg caccctgcca ctgtctgcaa
     1201 gataccccca ggctgcaacc tgaagatctt caacaaccag gaatttgctg ccctcctagc
     1261 tcagtctgtc aaccagggct ttgaggctgt ctaccagttg actcgcatgt gcaccatccg
     1321 catgagcttc gtcaaaggct ggggagccga gtacaggaga cagacagtga ccagcacccc
     1381 ctgctggatc gagctacacc tgaatgggcc cttgcagtgg cttgacaagg tcctcaccca
     1441 gatgggttcc cccagcatcc gctgttccag cgtgtcttag agacactggg agtaaaggga
     1501 tcgggttggg gagggcgggc ttggggaaaa tgaccttgga agataactcc atccaacttg
     1561 gtcttgtcaa agaacacaga ttctctcaac taaggcacta acctgtttct gaaaccacag
     1621 aagatgccag cgatggactt tatgaacagc tgtgtctgct gcatatatgt gccccaggct
     1681 gaaggccgag tgtgatggct tctgatctgg tggcttgagc taacaggtgg tgtgtcgcca
     1741 cctgactcct tgtttagtga cagagatatg ggatgtcaca gtccaaaaag aaagtgcctt
     1801 tctccaggac tggagtgtgg agttcacctt tggagaagaa cttttaatgg agcatgccct
     1861 gtctccccac tctcacagag agggtctgtg tacctaggga actggatggc ctacgcaggt
     1921 actttccccc acccccagaa tgcagatggg aacatgctgg agagtaaagc tggtgagaac
     1981 cgcttcttcc ttggaaggac ctgctgaagc tcagccatgt atgtgatgtg tagttcctag
     2041 tatcacatcg atctcaagag atacggatgt tcaagtgttc gcaacaaggg cagtcgtgga
     2101 gtacatgtcc tgggtgaggt ggtaccacat tgaatgaata catctctgga agctggcacc
     2161 actttctgat gtgtaggcca tgttggttta tggagacagc tgcattaatg aattcaccta
     2221 gcacaggctc tttggatgtc ttctgtttga tgaaaacagt cctgggtaca tatgttggct
     2281 ggaaaaccaa agaggaccca gtctctgctt cttgccctga ggtttggaaa ctgagagtta
     2341 tagaagacac ttcaccagga ggttaagaca tcccaggctg acatgggcaa atgaaagggc
     2401 tattaaagtt tttttacacc ttggaaaatt gcaggcttgg tgcagagacc tgtcatcttc
     2461 accctgggat gtaagattac ctggctccgg taaaggattg ccaccaaaaa ctgctgcggt
     2521 gatcctgtgt gatgagtact gttcgcagtg gcaatcacag agaaaatgag tataagttat
     2581 ttcaactgga aaaagagtcc ctttcctggc tgttccagaa cagcacatag gatcccacct
     2641 gcaactggta ctctcttctt ggctgctgtt tctgttgtga aatgaggagc ccatgttttc
     2701 gcatggattt cccgtggaag tcctgtcctg ttccagaggg gacaaaagtg caccctccaa
     2761 tgtgataaat cttctgatga tccagaggct ctggggcata tcctggtgcc tctcctgcag
     2821 gagctgtgcc ctcagaagct ctgggcagga tgtttccagc taggcatgct gatcctcctt
     2881 cagacacaga tgcctgtctg agtctctccc aggctgttga tacagaactc aaagacaatt
     2941 ctgaacaacc cctcaggttc tctgaagggg cacagacccc gggtgtgaac attgtgccgg
     3001 acttgacatg gtctgtggac tgctgcttca gacacaggaa gtcctcggaa ctgcctacct
     3061 gttctgcatg ctctgagtca gaacacagag acactttggt gtggcttcta gtattcatgg
     3121 tcctctcttc tcagccatgg cagaggttcg tgtcacttgc tgcctggtct cctaggggtt
     3181 tcctgtgccc gtccccccag ccatcttcca ggtgactatg gcacagtcca ttctgctgca
     3241 actggaaagt atagagaacc tgtactgctc agagcagacg gctgcttctg gaggcacgca
     3301 gtcgggagca tgccccgtca ctgtccagtt gctgaagaaa gcttacagga agttctaggc
     3361 aggtctactt tcggcatcct tgaaagtaaa aggtttggag tctaggaagg gccacgccac
     3421 tgtggagaac atagttcctt tgggtacatg taatcaaagt gtcctcccaa attagttttc
     3481 cttcttttat gaacagcatt tctactttta aagaacagtt gagaattgaa aggaaaacag
     3541 tgcagtagct ggtgtggcct ttaaagtgtg gttctgccag gtggcctgac tgcatgtgct
     3601 gacactgacc cacatggcta gtgctgggca gaggtctttg ccttcagcac cccagagaag
     3661 ctaactctgc acccctctcc ttcctcagcg cttgttcagg tagccccagc catgtggctt
     3721 atagtcatgt gctggcctgt catgggcagt tggctacctt ccagatggct gtagaaagac
     3781 caggcatttt gaggaaagcc tagagtggag agcagggact ttcttaggaa gaacacaagg
     3841 ctgctggcag acttctccac tgacctgaga gtgtgttgta tattttttta ctcagtaacg
     3901 tattgcatca agccttggtg cttatggggt gctgtggtct tgatcttccc cagtttgctt
     3961 ctcagagctg ggatgtattc cagaacctga tgtttttatt caaaccacag aaaggttgtt
     4021 tttaagtagt atgtgcagtc acgcatgtgc ctgagttgtc tggggcagag gcaagtatct
     4081 gacttcattc ttagccccag gctgccgttt ctgagtttca gtccttgaga ggctgggaag
     4141 gcacagagct ctggctctgc actgttctta ctgagtgact aggtgtgagc cctttacatt
     4201 agagggaacc tggtttgagc tcacttgtac tttgtgtggc gttagtgttc cattactggc
     4261 ccctctaagt aatggtcttc acagtgcaca gcaagttccc agtgtgtaga aagccataca
     4321 ccaggatgtg ggtcaaccat gaagatgtgg cattgcagac aggggaacat gtggatgcat
     4381 ggctatcacc ttgagcagcc cctgcagttg cttgtgttaa cacaaaagtg tttagcattc
     4441 tgccgctttt atatttatgt aataactctt taaagccatt cagatggata actatttaat
     4501 ttcttaaaga cagttgtaaa ggtctctctc tgaggacaat gacttggtaa aactggggca
     4561 cagccagtcc cagacactgg tcgtggctac agtggctttt tgggctcagg atcaacacgc
     4621 atcagagtag actgggccaa catgtggtgg cgtgtgcaaa caggctgccc tagaccagcc
     4681 caggcccttt gacagcacgt cctctgcacc aaggcccctc ccttctggaa gtggaaaact
     4741 tcacaaggaa attggagcca tcttttatcc cagaagcctt tgctgctgcc agggcaagtg
     4801 ggctggtgtg gactcttgtt tggggatgtt tggaggctga ggttatatca ctccttagct
     4861 agccaggcct ttagtatctt tgtaaaaagc atgtatttat agtgttttag attttttcta
     4921 acttttgtat tttacagcat tgtaccccat tgttaaagag ccatgtcccc tcttcttata
     4981 agcgcccttc taataaactt ttcaccgtaa agctcctgag ccaggagcgc ggtctga
//
