LOCUS       NM_001025281            2146 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus solute carrier family 9 member A8 (Slc9a8), mRNA.
ACCESSION   NM_001025281 XM_230865
VERSION     NM_001025281.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Zhou,K., Amiri,M., Salari,A., Yu,Y., Xu,H., Seidler,U. and
            Nikolovska,K.
  TITLE     Functional characterization of the sodium/hydrogen exchanger 8 and
            its role in proliferation of colonic epithelial cells
  JOURNAL   Am J Physiol Cell Physiol 321 (3), C471-C488 (2021)
   PUBMED   34288721
  REMARK    GeneRIF: Functional characterization of the sodium/hydrogen
            exchanger 8 and its role in proliferation of colonic epithelial
            cells.
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Wiebe,S.A., Plain,A., Pan,W., O'Neill,D., Braam,B. and
            Alexander,R.T.
  TITLE     NHE8 attenuates Ca2+ influx into NRK cells and the proximal tubule
            epithelium
  JOURNAL   Am J Physiol Renal Physiol 317 (2), F240-F253 (2019)
   PUBMED   31042050
  REMARK    GeneRIF: NHE8 regulates Ca(2+) uptake into the proximal tubule
            epithelium.
REFERENCE   3  (bases 1 to 2146)
  AUTHORS   Joseph,C., Twombley,K., Gattineni,J., Zhang,Q., Dwarakanath,V. and
            Baum,M.
  TITLE     Acid increases NHE8 surface expression and activity in NRK cells
  JOURNAL   Am J Physiol Renal Physiol 302 (4), F495-F503 (2012)
   PUBMED   22088432
REFERENCE   4  (bases 1 to 2146)
  AUTHORS   Xu,H., Zhang,B., Li,J., Chen,H., Tooley,J. and Ghishan,F.K.
  TITLE     Epidermal growth factor inhibits intestinal NHE8 expression via
            reducing its basal transcription
  JOURNAL   Am J Physiol Cell Physiol 299 (1), C51-C57 (2010)
   PUBMED   20375273
  REMARK    GeneRIF: EGF inhibits intestinal NHE8 gene expression by reducing
            its basal transcription.
REFERENCE   5  (bases 1 to 2146)
  AUTHORS   Xu,H., Chen,H., Dong,J., Li,J., Chen,R., Uno,J.K. and Ghishan,F.K.
  TITLE     Tumor necrosis factor-{alpha} downregulates intestinal NHE8
            expression by reducing basal promoter activity
  JOURNAL   Am J Physiol Cell Physiol 296 (3), C489-C497 (2009)
   PUBMED   19109523
  REMARK    GeneRIF: Tumor necrosis factor-alpha downregulates intestinal NHE8
            expression by reducing basal promoter activity.
REFERENCE   6  (bases 1 to 2146)
  AUTHORS   Gattineni,J., Sas,D., Dagan,A., Dwarakanath,V. and Baum,M.
  TITLE     Effect of thyroid hormone on the postnatal renal expression of NHE8
  JOURNAL   Am J Physiol Renal Physiol 294 (1), F198-F204 (2008)
   PUBMED   17977906
  REMARK    GeneRIF: Thyroid hormone plays a potential role in the
            developmental isoform change from NHE8 to NHE3 and decreases NHE8
            activity.
REFERENCE   7  (bases 1 to 2146)
  AUTHORS   Xu,H., Chen,H., Dong,J., Lynch,R. and Ghishan,F.K.
  TITLE     Gastrointestinal distribution and kinetic characterization of the
            sodium-hydrogen exchanger isoform 8 (NHE8)
  JOURNAL   Cell Physiol Biochem 21 (1-3), 109-116 (2008)
   PUBMED   18209477
  REMARK    GeneRIF: NHE8 is expressed along the gastrointestinal tract and
            NHE8 is a functional Na(+)/H(+) exchanger with kinetic
            characteristics that differ from other apically expressed NHE
            isoforms.
REFERENCE   8  (bases 1 to 2146)
  AUTHORS   Zhang,J., Bobulescu,I.A., Goyal,S., Aronson,P.S., Baum,M.G. and
            Moe,O.W.
  TITLE     Characterization of Na+/H+ exchanger NHE8 in cultured renal
            epithelial cells
  JOURNAL   Am J Physiol Renal Physiol 293 (3), F761-F766 (2007)
   PUBMED   17581925
  REMARK    GeneRIF: NHE8 protein is expressed in the apical membrane of NRK
            cells as demonstrated by immunoblots, confocal fluorescent
            immunocytochemistry, and immunoelectron microscopy.
REFERENCE   9  (bases 1 to 2146)
  AUTHORS   Xu,H., Chen,R. and Ghishan,F.K.
  TITLE     Subcloning, localization, and expression of the rat intestinal
            sodium-hydrogen exchanger isoform 8
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 289 (1), G36-G41 (2005)
   PUBMED   15731506
  REMARK    GeneRIF: NHE-8 may play an important role in intestinal Na+
            absorption during early life
REFERENCE   10 (bases 1 to 2146)
  AUTHORS   Nakamura,N., Tanaka,S., Teko,Y., Mitsui,K. and Kanazawa,H.
  TITLE     Four Na+/H+ exchanger isoforms are distributed to Golgi and
            post-Golgi compartments and are involved in organelle pH regulation
  JOURNAL   J Biol Chem 280 (2), 1561-1572 (2005)
   PUBMED   15522866
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On May 18, 2021 this sequence version replaced NM_001025281.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY496958.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                JAXUCZ010000003.1  176567125-176567165
            42-220              JAXUCZ010000003.1  176569921-176570099
            221-301             JAXUCZ010000003.1  176578900-176578980
            302-360             JAXUCZ010000003.1  176585620-176585678
            361-444             JAXUCZ010000003.1  176587906-176587989
            445-546             JAXUCZ010000003.1  176590398-176590499
            547-581             JAXUCZ010000003.1  176591418-176591452
            582-725             JAXUCZ010000003.1  176595476-176595619
            726-864             JAXUCZ010000003.1  176598477-176598615
            865-970             JAXUCZ010000003.1  176600813-176600918
            971-1087            JAXUCZ010000003.1  176606407-176606523
            1088-1170           JAXUCZ010000003.1  176608556-176608638
            1171-1282           JAXUCZ010000003.1  176609736-176609847
            1283-1503           JAXUCZ010000003.1  176611892-176612112
            1504-1650           JAXUCZ010000003.1  176613962-176614108
            1651-2146           JAXUCZ010000003.1  176614595-176615090
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q42"
     gene            1..2146
                     /gene="Slc9a8"
                     /note="solute carrier family 9 member A8"
                     /db_xref="GeneID:311651"
                     /db_xref="RGD:1308193"
     exon            1..41
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1758
                     /gene="Slc9a8"
                     /note="NHE-8; solute carrier family 9 member 8; Na(+)/H(+)
                     exchanger 8; solute carrier family 9 (sodium/hydrogen
                     exchanger), member 8; solute carrier family 9, subfamily A
                     (NHE8, cation proton antiporter 8), member 8"
                     /codon_start=1
                     /product="sodium/hydrogen exchanger 8"
                     /protein_id="NP_001020452.2"
                     /db_xref="GeneID:311651"
                     /db_xref="RGD:1308193"
                     /translation="MAEEFSNTTHESFNFTLHTTLGVTTKLVLPTPAKPILPVQTGEQ
                     AQQEEQSSGMTIFFSLLVLAICIILVHLLIRYRLHFLPESVAVVSLGILMGAVIKVIE
                     FKKLANWKEEEMFRPNMFFLLLLPPIIFESGYSLHKGNFFQNIGSITLFAVFGTAISA
                     FVVGGGIYFLGQADVISKLNMTDSFAFGSLISAVDPVATIAIFNALHVDPVLNMLVFG
                     ESILNDAVSIVLTNTAEGLTRKHMSDVSGWQTFSQALGYFLKMFFGSAALGTLTGLIS
                     ALVLKHIDLRKTPSLEFGMMIIFAYLPYGLAEGISLSGIMAILFSGIVMSHYTHHNLS
                     PVTQILMQQTLRTVAFLCETCVFAFLGLSIFSFPHKFEISFVIWCIVLVLFGRAVNIF
                     PLSYLLNFFRDHKITPKMMFIMWFSGLRGAIPYALSLHLGLEPMEKRQLIGTTTIIIV
                     LFTILLLGGSTMPLIRLVDIEDARARRRSKKDVNLSKTEKMGNAIESEHLSELTEEEY
                     EAHYIRQQDLKGFMWLDAKYLNPFFTRRLTQEDLHHGRIQMKSLTNKWYEEVRQGPSG
                     SEDDEQELF"
     exon            42..220
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            221..301
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            302..360
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            361..444
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            445..546
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            547..581
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            582..725
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            726..864
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            865..970
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1087
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1170
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1282
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1503
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1650
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..2146
                     /gene="Slc9a8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtctccgaag ctcgcaggac tggggtgaag atggcggagg agttctccaa cacgacccat
       61 gagagcttca acttcaccct tcacaccact ttgggtgtca ccacgaagct ggtgctgcca
      121 acaccagcca agcctattct tccggtgcag acaggagagc aggcccagca ggaggagcag
      181 tcaagtggca tgaccatttt cttcagcctt ctcgtcctag ccatctgcat catcctggtg
      241 cacctgctga ttcggtaccg actgcacttc ctgcctgaga gcgtggctgt tgtttctcta
      301 ggcattctca tgggagcagt tataaaagtt atagagttta agaagctggc aaattggaag
      361 gaggaagaaa tgtttcgccc caacatgttt ttccttctct tgctcccacc tatcatcttt
      421 gagtcgggat actcactgca taaggggaac ttctttcaaa acattggctc catcactctc
      481 ttcgctgtct ttggaacagc aatctcagcc tttgtagtag gtggaggaat ttacttcctg
      541 ggtcaggctg atgtaatctc taaactcaac atgacagaca gtttcgcgtt tggctccctg
      601 atatctgcag ttgacccggt tgccactatt gcaattttca atgcactgca cgtggaccct
      661 gtcctcaaca tgctggtgtt tggagaaagc attctcaacg atgcagtctc cattgtcctc
      721 accaacacgg ctgaaggtct aacaagaaaa catatgtcag atgtcagtgg gtggcaaaca
      781 ttctcacaag ccctcggcta cttcctcaaa atgttctttg gctctgcagc gcttggcacg
      841 ctgactggct tgatttcagc attagtgttg aagcacattg acctgaggaa gacgccttcc
      901 ctggagtttg gcatgatgat catttttgct tacctgccat atggcctggc ggagggtatc
      961 tcactctcag gcatcatggc gatcctgttt tctggcatcg tgatgtcaca ctacacacat
     1021 cataacctct ccccagtcac tcagatcctc atgcagcaga ccctccggac tgtggccttc
     1081 ctgtgtgaaa catgtgtgtt tgcatttctt ggcctctcca tttttagttt ccctcacaag
     1141 tttgaaattt cctttgtcat ctggtgcata gtgctggttc tgttcggcag agctgtcaac
     1201 atctttcctc tgtcttacct gctgaacttc ttccgggatc acaaaatcac ccccaagatg
     1261 atgttcatca tgtggttcag tggtcttcga ggggccattc cctatgcgct gagcctgcat
     1321 ctgggtctgg agcccatgga gaagcgccag ctcataggca ccaccaccat catcattgtg
     1381 ctcttcacca tcctgctgct gggcggcagc accatgcccc tcatccgcct cgtggacatc
     1441 gaggacgccc gggctcgccg aaggagcaag aaagatgtga acctcagcaa gactgagaag
     1501 atgggaaatg ctatcgagtc agaacacctg tctgagctca ctgaggagga gtacgaagct
     1561 cactacatca gacagcagga cctcaaaggc ttcatgtggt tggatgccaa gtatctgaac
     1621 cccttcttta cacggcggct gacacaggag gacctccatc acgggcgtat ccagatgaaa
     1681 agcctcacca acaaatggta tgaggaggtc cgtcagggac catcgggctc tgaggatgat
     1741 gagcaggagc tgttctgagc ccacagtgcc tcccagagcc tcctcagaac acaagatggt
     1801 aggtcaaggg ctagtcctag ctaggaagct tcaggcctct ggagcatgct ggcttccgaa
     1861 atgtgatagg cctctatgac tcatggaacc agacagaccc aggccagtgt tgttcctggg
     1921 cctgtagagg atggcagtcg ccagcctcct cctgcctctc tgccaggcca cagagaaaac
     1981 tttgggaggc tgcttgctca tctcacacct gtctgggcca tgccttcctc acacctctgg
     2041 agcagctgct ggcgggcaca tctgccattg tctctgaatt gtcaaggtgc cttggtgtcg
     2101 gcagcagcaa cttcgacctg taccaaggcc aagagactct gaagcc
//
