LOCUS       NM_001113335            3942 bp    mRNA    linear   ROD 02-APR-2024
DEFINITION  Rattus norvegicus solute carrier family 9 member A2 (Slc9a2),
            transcript variant 1, mRNA.
ACCESSION   NM_001113335
VERSION     NM_001113335.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Paehler Vor der Nolte,A., Chodisetti,G., Yuan,Z., Busch,F.,
            Riederer,B., Luo,M., Yu,Y., Menon,M.B., Schneider,A., Stripecke,R.,
            Nikolovska,K., Yeruva,S. and Seidler,U.
  TITLE     Na+ /H+ exchanger NHE1 and NHE2 have opposite effects on migration
            velocity in rat gastric surface cells
  JOURNAL   J Cell Physiol 232 (7), 1669-1680 (2017)
   PUBMED   28019659
  REMARK    GeneRIF: The results demonstrate that in RGM1 cells, migratory
            velocity is increased by NHE1 activation, while NHE2 activity
            inhibit this process.
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Soleiman,A.A., Thameem,F. and Khan,I.
  TITLE     Mechanism of down regulation of Na-H exchanger-2 in experimental
            colitis
  JOURNAL   PLoS One 12 (5), e0176767 (2017)
   PUBMED   28493993
  REMARK    GeneRIF: These findings demonstrate suppression of NHE-2 expression
            on the brush borders in the colonic epithelial cells which is
            regulated transcriptionally.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3942)
  AUTHORS   Rivarola,V., Di Giusto,G., Christensen,M.J., Ford,P. and Capurro,C.
  TITLE     AQP2-Induced Acceleration of Renal Cell Proliferation Involves the
            Activation of a Regulatory Volume Increase Mechanism Dependent on
            NHE2
  JOURNAL   J Cell Biochem 118 (5), 967-978 (2017)
   PUBMED   27191152
  REMARK    GeneRIF: Results provide evidence that NHE2 and AQP2 work in
            coordination as part of the cellular device that regulates
            collecting duct cell cycle progression.
REFERENCE   4  (bases 1 to 3942)
  AUTHORS   Rajendran,V.M., Nanda Kumar,N.S., Tse,C.M. and Binder,H.J.
  TITLE     Na-H Exchanger Isoform-2 (NHE2) Mediates Butyrate-dependent Na+
            Absorption in Dextran Sulfate Sodium (DSS)-induced Colitis
  JOURNAL   J Biol Chem 290 (42), 25487-25496 (2015)
   PUBMED   26350456
  REMARK    GeneRIF: observations suggest that the butyrate activation of NHE2
            would be a potential target to control UC-associated diarrhea
REFERENCE   5  (bases 1 to 3942)
  AUTHORS   Ismail,N., Giribabu,N., Muniandy,S. and Salleh,N.
  TITLE     Enhanced Expression of Sodium Hydrogen Exchanger (NHE)-1, 2 and 4
            in the Cervix of Ovariectomised Rats by Phytoestrogen Genistein
  JOURNAL   Int J Med Sci 12 (6), 468-477 (2015)
   PUBMED   26078707
  REMARK    GeneRIF: Enhanced expression of NHE-1, 2 and 4 proteins and mRNAs
            expression in cervix under genistein influence could help to
            restore the cervicovaginal fluid pH that might help to prevent
            cervicovaginal complications related to menopause
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3942)
  AUTHORS   Muller,Y.L., Collins,J.F., Bai,L., Xu,H. and Ghishan,F.K.
  TITLE     Molecular cloning and characterization of the rat NHE-2 gene
            promoter
  JOURNAL   Biochim Biophys Acta 1442 (2-3), 314-319 (1998)
   PUBMED   9804979
REFERENCE   7  (bases 1 to 3942)
  AUTHORS   Ghishan,F.K., Knobel,S.M. and Summar,M.
  TITLE     Molecular cloning, sequencing, chromosomal localization, and tissue
            distribution of the human Na+/H+ exchanger (SLC9A2)
  JOURNAL   Genomics 30 (1), 25-30 (1995)
   PUBMED   8595899
REFERENCE   8  (bases 1 to 3942)
  AUTHORS   Yu,F.H., Shull,G.E. and Orlowski,J.
  TITLE     Functional properties of the rat Na/H exchanger NHE-2 isoform
            expressed in Na/H exchanger-deficient Chinese hamster ovary cells
  JOURNAL   J Biol Chem 268 (34), 25536-25541 (1993)
   PUBMED   8244989
REFERENCE   9  (bases 1 to 3942)
  AUTHORS   Wang,Z., Orlowski,J. and Shull,G.E.
  TITLE     Primary structure and functional expression of a novel
            gastrointestinal isoform of the rat Na/H exchanger
  JOURNAL   J Biol Chem 268 (16), 11925-11928 (1993)
   PUBMED   7685026
REFERENCE   10 (bases 1 to 3942)
  AUTHORS   Collins,J.F., Honda,T., Knobel,S., Bulus,N.M., Conary,J., DuBois,R.
            and Ghishan,F.K.
  TITLE     Molecular cloning, sequencing, tissue distribution, and functional
            expression of a Na+/H+ exchanger (NHE-2)
  JOURNAL   Proc Natl Acad Sci U S A 90 (9), 3938-3942 (1993)
   PUBMED   7683411
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            L11236.1, L11004.1 and BE117286.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132291,
                              SAMD00132295 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-962               L11236.1           1-962
            963-1193            L11004.1           975-1205
            1194-3293           L11236.1           1194-3293
            3294-3854           L11004.1           3306-3866
            3855-3942           BE117286.1         1-88                c
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q22"
     gene            1..3942
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /note="solute carrier family 9 member A2"
                     /db_xref="GeneID:24783"
                     /db_xref="RGD:3719"
     exon            1..478
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     CDS             187..2628
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; sodium/hydrogen exchanger 2; Na/H ion
                     exchanger; H7; NHE-2; Na(+)/H(+) exchanger 2; Solute
                     carrier family 9 (sodium/hydrogen exchanger 2), antiporter
                     2, Na+/H+ (Na+/H+ exchanger 2); solute carrier family 9
                     (sodium/hydrogen exchanger), member 2; solute carrier
                     family 9, subfamily A (NHE2, cation proton antiporter 2),
                     member 2; solute carrier family 9 member 2"
                     /codon_start=1
                     /product="sodium/hydrogen exchanger 2 isoform 1 precursor"
                     /protein_id="NP_001106806.1"
                     /db_xref="GeneID:24783"
                     /db_xref="RGD:3719"
                     /translation="MGPSGTAHRMRAPLSWLLLLLLSLQVAVPAGALAETLLDAPGAR
                     GASSNPPSPASVVAPGTTPFEESRLPVFTLDYPHVQIPFEITLWILLASLAKIGFHLY
                     HKLPTIVPESCLLIMVGLLLGGIIFGVDEKSPPAMKTDVFFLYLLPPIVLDAGYFMPT
                     RPFFENLGTIFWYAVVGTLWNSIGIGLSLFGICQIEAFGLSDITLLQNLLFGSLISAV
                     DPVAVLAVFENIHVNEQLYILVFGESLLNDAVTVVLYNLFKSFCQMKTIQTVDVFAGI
                     ANFFVVGIGGVLIGILLGFIAAFTTRFTHNIRVIEPLFVFLYSYLSYITAEMFHLSGI
                     MAITACAMTMNKYVEENVSQKSYTTIKYFMKMLSSVSETLIFIFMGVSTVGKNHEWNW
                     AFVCFTLAFCLIWRALGVFVLTQVINWFRTIPLTFKDQFIIAYGGLRGAICFALVFLL
                     PATVFPRKKLFITAAIVVIFFTVFILGITIRPLVEFLDVKRSNKKQQAVSEEIHCRFF
                     DHVKTGIEDVCGHWGHNFWRDKFKKFDDKYLRKLLIRENQPKSSIVSLYKKLEIKHAI
                     EMAETGMISTVPSFASLNDCREEKIRKLTPGEMDEIREILSRNLYQIRQRTLSYNRHN
                     LTADTSERQAKEILIRRRHSLRESLRKDNSLNRERRASTSTSRYLSLPKNTKLPEKLQ
                     KKNKVSNADGNSSDSDMDGTTVLNLQPRARRFLPDQFSKKASPAYKMEWKNEVDVGSA
                     RAPPSVTPAPRSKEGGTQTPGVLRQPLLSKDQRFGRGREDSLTEDVPPKPPPRLVRRA
                     SEPGNRKGRLGNEKP"
     exon            479..942
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1193
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1411
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..1614
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1704
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1775
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1937
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2034
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2166
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2257
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     exon            2258..3932
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3383
                     /gene="Slc9a2"
                     /gene_synonym="Nhe2"
ORIGIN      
        1 cccctccggg ccctgcgagg ttcacagcca cagagcttgc tggcggcagg gcccctgccc
       61 cgtggaggct gtggcgtggt actctcgcca tcccgttgag ttaatagcgc tgtcccccag
      121 cgatgcgctg agggtgccca gatccctaca ctggcgtcct ctgctgcgcc tagagccacc
      181 gaccccatgg gcccctcagg cactgcgcac agaatgcggg cgccactgtc ttggctgctg
      241 ctgctgctgc tgtctctgca ggtggcagtg ccggcgggcg cactggctga gaccctgctg
      301 gacgcacccg gggccagggg tgccagctcc aacccgccaa gccccgccag tgtggtggcc
      361 ccggggacaa cgccgttcga ggaaagtcga ttgcccgtgt tcaccctgga ttacccccac
      421 gtgcaaatcc ctttcgagat cactttgtgg atcctactgg cttccttggc caagattggt
      481 ttccatctct atcacaagtt gcccacgatt gtgccggaga gctgtcttct cataatggtt
      541 gggcttctac taggtgggat tatcttcggt gtcgatgaga agtctcctcc tgcaatgaaa
      601 acggatgtat ttttcctata cctcctgcca cccattgttc tggatgctgg ctacttcatg
      661 cctactcgac ctttctttga gaacttgggc accattttct ggtatgctgt ggtaggcaca
      721 ctgtggaatt ccattggcat tgggctgtct ttgttcggca tctgccagat tgaggcgttt
      781 ggcctcagcg acatcacctt gctccagaac ctgctcttcg gcagcctgat ctcagctgtg
      841 gaccctgtgg ctgtgcttgc tgtctttgag aacattcatg tcaatgaaca gctctatatc
      901 ctggtttttg gcgagtctct gctcaacgat gcagtcacag tggtcctgta caacttgttc
      961 aaatccttct gccagatgaa gaccatccag accgtagacg tgtttgctgg catcgctaac
     1021 ttcttcgtag tggggattgg cggggtgctg attggtatcc tgctgggatt catagcagcg
     1081 ttcaccaccc gtttcacaca caacatccgg gtcattgagc cgctctttgt cttcctgtac
     1141 agctacttgt cctacatcac tgctgaaatg ttccaccttt caggcatcat ggcgatcaca
     1201 gcctgtgcca tgacgatgaa caagtatgtg gaagagaacg tgtcccagaa gtcctacacg
     1261 accatcaagt acttcatgaa gatgctgagc agcgtgagtg agaccctcat cttcatcttc
     1321 atgggcgtgt ccaccgttgg gaagaaccat gagtggaact gggctttcgt ctgcttcacc
     1381 ctggccttct gcctgatctg gcgagcactg ggtgtctttg tcctgactca ggtgattaat
     1441 tggttccgga ccatcccact gaccttcaag gaccagttca tcattgccta tggagggctc
     1501 cgcggggcca tctgctttgc actggtgttc ctcctccctg ctactgtgtt tccccgaaag
     1561 aagctgttca tcacagctgc catcgtcgtt atattcttca ctgtcttcat cctgggaata
     1621 accatccggc cactggtgga atttcttgat gttaagagat ccaataagaa gcagcaagct
     1681 gtcagtgaag agatccactg tcggtttttt gatcatgtga agactgggat tgaagatgtt
     1741 tgtggacact ggggtcacaa cttctggaga gacaagttta agaaatttga tgacaaatac
     1801 cttcggaagc ttctgattcg ggaaaaccaa cccaagtcta gcattgtgtc cttatataaa
     1861 aagcttgaaa taaaacatgc catagagatg gcagagacag ggatgataag cactgtcccc
     1921 tcatttgcat ctctcaacga ctgccgtgaa gagaaaataa ggaagctcac gccaggagaa
     1981 atggatgaaa ttagagagat attgtcaagg aatctctatc aaatccgcca gcggaccttg
     2041 tcctacaaca gacacaacct gacggcagac acaagtgaga ggcaagccaa ggagatcttg
     2101 attcgccgac ggcacagtct tcgggaaagt cttaggaagg acaacagctt aaaccgcgag
     2161 cgcagggctt ctacttcaac ctcccgatat ttatccttac ctaaaaacac aaagcttccc
     2221 gagaagttgc agaagaagaa taaggtttca aatgcagatg gtaatagcag cgactcggac
     2281 atggatggaa ccactgtact caatttgcag cccagggcca ggcgcttctt gccagatcag
     2341 ttctcaaaga aagcctcacc agcctacaaa atggaatgga agaatgaggt ggacgtgggt
     2401 tctgcgcgag cgccccccag cgtcactcca gcgcctcgca gtaaagaggg gggcacccag
     2461 acaccagggg tgctgaggca gcccctgctc tccaaagacc aacggtttgg ccggggcagg
     2521 gaagacagtt tgactgaaga tgttccaccc aagcccccac caaggttggt gcgacgagca
     2581 tcagaacccg gaaataggaa aggccgcctt ggcaacgaaa agccatgata tgaagccaaa
     2641 gcagacttgt ggtgaatggg caagcatggt tgtgacttgt tactcaaacc caatcgaccg
     2701 tggaatccac ttaaagtcct ctatccagag gcattctttg ggaagtagag tcaagccact
     2761 gattcatggg aggagcgctg cttatgtatc ccaagaagag gaaggcagag gaaggaagtc
     2821 ccacaaaatg acttttgtga cttttggcta gtaaatgtat tctttcaagg cctgaaagga
     2881 aaaaaaaatg tgtgccttta tcgaacttga atgacagaac ttgaaaattt taacgcacct
     2941 ttgttggtga aaattttaat atattacaaa tatgcctcag atttatttat gaaaaaaatg
     3001 tgtttatctg tagcgatctt ttttttccca agggaatgaa aacgtggcaa gaacattttt
     3061 tccaaggggg taaaggtcag ctcctagagg tgggtcttaa gatccagatc ccaccatccc
     3121 atcctgagct gatggaccac ttggtggagt agaacactgt ccccagcaga ggccttggct
     3181 gacggtatta gggcacaggt tggaatgtat aggcagatga aatgccctga ctctcagagt
     3241 gtgggtggga agtccctgta caactttgaa acaaggaggt tcagactgct ctcagtccat
     3301 tcctgtgttt tccatggctt tggagtaaca tgtaatctcc acccccctcc cttacccccg
     3361 cctctgtccc aatttttatc taggtacaga aagattcact tgcacaaaat cagcccatag
     3421 tgtttataga cttggtggca gcttccgaga agtcctacca gtgaggacct cttgtggcca
     3481 gtgtgagggc agccactagt gaccgcctgg gttttatgtg tgtggtgatg ggaggaaagg
     3541 gaggtggttc ctttcattag ggatccatgg ccacctgctt taataaaatt gtcctgcagt
     3601 cccttcagag ctgcgagctg ggaagggtca acgtttatac gaaccgcttt ccagtgttaa
     3661 agaaaatggt gggtctttgg gtttgacttc gaatgaggaa gcactttgga gaaaccaccc
     3721 actttttgca ctagttttta atttgttacc tcttcacctt tgatgatgta gtgactcaaa
     3781 tttgtcatta aaaaaattag attccattct ctggaatact ttttaggagt atggcagaga
     3841 ggaaacagtg ggacacaatc attaaagatt catatttaaa ccaatgcctc tcaagctgca
     3901 gatttattat taaagattca tatttaaaca aaaaaaaaaa aa
//
