LOCUS       NM_053442               4117 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus solute carrier family 7 member 8 (Slc7a8), mRNA.
ACCESSION   NM_053442
VERSION     NM_053442.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Wu,Y., Yin,Q., Lin,S., Huang,X., Xia,Q., Chen,Z., Zhang,X. and
            Yang,D.
  TITLE     Increased SLC7A8 expression mediates L-DOPA uptake by renal tubular
            epithelial cells
  JOURNAL   Mol Med Rep 16 (1), 887-893 (2017)
   PUBMED   28560461
  REMARK    GeneRIF: The results of the present study suggested that SLC7A8 may
            serve a critical role in blood pressure control through regulating
            LDOPA uptake in renal epithelial cells of the proximal tubule.
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Matsuyama,R., Tomi,M., Akanuma,S., Tabuchi,A., Kubo,Y.,
            Tachikawa,M. and Hosoya,K.
  TITLE     Up-regulation of L-type amino acid transporter 1 (LAT1) in cultured
            rat retinal capillary endothelial cells in response to glucose
            deprivation
  JOURNAL   Drug Metab Pharmacokinet 27 (3), 317-324 (2012)
   PUBMED   22185814
  REMARK    GeneRIF: Data suggest that glucose deprivation sensitivity (model
            for ischemia) of LAT1 expression in retinal capillary endothelial
            cells is transcriptionally regulated, and cis-elements within LAT1
            promoter region from -162 bp to -155 bp mediate this regulation.
REFERENCE   3  (bases 1 to 4117)
  AUTHORS   Zielinska,M., Ruszkiewicz,J., Hilgier,W., Fresko,I. and Albrecht,J.
  TITLE     Hyperammonemia increases the expression and activity of the
            glutamine/arginine transporter y+ LAT2 in rat cerebral cortex:
            implications for the nitric oxide/cGMP pathway
  JOURNAL   Neurochem Int 58 (2), 190-195 (2011)
   PUBMED   21115085
  REMARK    GeneRIF: Reduced delivery of Arg due to enhanced y+ LAT2-mediated
            exchange of extracellular Gln for intracellular Arg may contribute
            to the decrease of nitric oxide/cyclic GMP pathway activity evoked
            in the brain by hyperammonemia.
REFERENCE   4  (bases 1 to 4117)
  AUTHORS   Kim,S.G., Ahn,Y.C., Yoon,J.H., Kim,H.K., Park,S.S., Ahn,S.G.,
            Endou,H., Kanai,Y., Park,J.C. and Kim,D.K.
  TITLE     Expression of amino acid transporter LAT1 and 4F2hc in the healing
            process after the implantation of a tooth ash and plaster of Paris
            mixture
  JOURNAL   In Vivo 20 (5), 591-597 (2006)
   PUBMED   17091765
  REMARK    GeneRIF: LAT1 and its subunit 4F2hc are highly expressed in the
            early stage of new bone formation and may have an important role in
            providing cells with neutral amino acids, including several
            essential amino acids, at that stage.
REFERENCE   5  (bases 1 to 4117)
  AUTHORS   Park,S.Y., Kim,J.K., Kim,I.J., Choi,B.K., Jung,K.Y., Lee,S.,
            Park,K.J., Chairoungdua,A., Kanai,Y., Endou,H. and Kim,D.K.
  TITLE     Reabsorption of neutral amino acids mediated by amino acid
            transporter LAT2 and TAT1 in the basolateral membrane of proximal
            tubule
  JOURNAL   Arch Pharm Res 28 (4), 421-432 (2005)
   PUBMED   15918515
REFERENCE   6  (bases 1 to 4117)
  AUTHORS   Pinho,M.J., Gomes,P., Serrao,M.P., Bonifacio,M.J. and
            Soares-da-Silva,P.
  TITLE     Organ-specific overexpression of renal LAT2 and enhanced tubular
            L-DOPA uptake precede the onset of hypertension
  JOURNAL   Hypertension 42 (4), 613-618 (2003)
   PUBMED   12975385
  REMARK    GeneRIF: Overexpression of renal LAT2 might contribute to enhanced
            L-DOPA uptake and precedes the onset of hypertension.
REFERENCE   7  (bases 1 to 4117)
  AUTHORS   Simmons-Willis,T.A., Koh,A.S., Clarkson,T.W. and Ballatori,N.
  TITLE     Transport of a neurotoxicant by molecular mimicry: the
            methylmercury-L-cysteine complex is a substrate for human L-type
            large neutral amino acid transporter (LAT) 1 and LAT2
  JOURNAL   Biochem J 367 (Pt 1), 239-246 (2002)
   PUBMED   12117417
REFERENCE   8  (bases 1 to 4117)
  AUTHORS   Rossier,G., Meier,C., Bauch,C., Summa,V., Sordat,B., Verrey,F. and
            Kuhn,L.C.
  TITLE     LAT2, a new basolateral 4F2hc/CD98-associated amino acid
            transporter of kidney and intestine
  JOURNAL   J Biol Chem 274 (49), 34948-34954 (1999)
   PUBMED   10574970
REFERENCE   9  (bases 1 to 4117)
  AUTHORS   Segawa,H., Fukasawa,Y., Miyamoto,K., Takeda,E., Endou,H. and
            Kanai,Y.
  TITLE     Identification and functional characterization of a Na+-independent
            neutral amino acid transporter with broad substrate selectivity
  JOURNAL   J Biol Chem 274 (28), 19745-19751 (1999)
   PUBMED   10391916
REFERENCE   10 (bases 1 to 4117)
  AUTHORS   Pineda,M., Fernandez,E., Torrents,D., Estevez,R., Lopez,C.,
            Camps,M., Lloberas,J., Zorzano,A. and Palacin,M.
  TITLE     Identification of a membrane protein, LAT-2, that Co-expresses with
            4F2 heavy chain, an L-type amino acid transport activity with broad
            specificity for small and large zwitterionic amino acids
  JOURNAL   J Biol Chem 274 (28), 19738-19744 (1999)
   PUBMED   10391915
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB024400.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB024400.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760434 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p13"
     gene            1..4117
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /note="solute carrier family 7 member 8"
                     /db_xref="GeneID:84551"
                     /db_xref="RGD:619904"
     exon            1..754
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /inference="alignment:Splign:2.1.0"
     CDS             601..2202
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /note="L-type amino acid transporter 2; cationic amino
                     acid transporter y+ system; solute carrier family 7
                     (cationic amino acid transporter, y+ system), member 8;
                     solute carrier family 7 (amino acid transporter light
                     chain, L system), member 8"
                     /codon_start=1
                     /product="large neutral amino acids transporter small
                     subunit 2"
                     /protein_id="NP_445894.1"
                     /db_xref="GeneID:84551"
                     /db_xref="RGD:619904"
                     /translation="MEKGTRQRNNTAKNHPDRGSDTSPEAEASSGGGGVALKKEIGLV
                     SACGIIVGNIIGSGIFVSPKGVLENAGSVGLALIVWIVTGVITAVGALCYAELGVTIP
                     KSGGDYSYVKDIFGGLAGFLRLWIAVLVIYPTNQAVIALTFSNYVLQPLFPTCFPPES
                     GLRLLAAICLLLLTWVNCSSVRWATRVQDIFTAGKLLALALIIIMGVVQICKGEFFWL
                     EPKNAFENFQEPDIGLVALAFLQGSFAYGGWNFLNYVTEELVDPYKNLPRAIFISIPL
                     VTFVYVFANIAYVTAMSPQELLASNAVAVTFGEKLLGVMAWIMPISVALSTFGGVNGS
                     LFTSSRLFFAGAREGHLPSVLAMIHVKRCTPIPALLFTCLSTLLMLVTSDMYTLINYV
                     GFINYLFYGVTVAGQIVLRWKKPDIPRPIKISLLFPIIYLLFWAFLLIFSLWSEPVVC
                     GIGLAIMLTGVPVYFLGVYWQHKPKCFNDFIESLTLVSQKMCVVVYPQEGDSGTEETI
                     DDVEEQHKPIFQPTPVKDPDSEEQP"
     exon            755..959
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1111
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1237
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1391
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1515
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1619
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1716
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1866
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /inference="alignment:Splign:2.1.0"
     exon            1867..2044
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /inference="alignment:Splign:2.1.0"
     exon            2045..4102
                     /gene="Slc7a8"
                     /gene_synonym="Lat2; Lat4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccaccctgc cctcacagat ggaacgatct gcggcgtgag cacggagctc ggagcggaca
       61 agggcgagaa ggggcgaccg cagcaggtgg aaacctgatc gcgagccggc gagcgagaag
      121 aaagtacctg agcaccgctc ctcctccgcc ctctcggcta acgagaacta cttttaccct
      181 cgtggtaaaa gagacttaag ggaaggtgga ggcgatctgt ttcgttagga gggctgcagg
      241 gaaattctga ccagagcaag ccgccgcccg gtacaccgag cagagccatc ctgggcttct
      301 gaagataaac gctttccgga gaattccagc tggagtcgct ggtacagttc tatttttgta
      361 tttagatata tatgttcttt gccttccatt taaaaagcac ggcccttgtt tgttagcagt
      421 gacacgtgtg actctcgttt gctgtcactt ttagagccta ggagcgctcg ctctccgttt
      481 ctgaaaaggg cgttctaatt ggaaacttgt ttttccgcgt atctctgcat ttatacgcac
      541 gagcgtttag aaagagactg ctctttttag cttttggtgg tcggaaccct acggagaaag
      601 atggaaaagg gaacccgaca gcgaaacaac accgcgaaga accacccgga ccgcggatct
      661 gacaccagcc ctgaggccga ggctagctcg ggagggggcg gagtagccct gaagaaagag
      721 atcggattgg tcagcgcctg tggtatcatt gtagggaaca tcattggctc cggaatcttc
      781 gtctcaccaa agggtgtgct ggagaacgct ggctcggtgg gccttgctct cattgtctgg
      841 atcgtgacgg gtgtcatcac ggctgtggga gctctctgct atgctgaact aggtgtcacc
      901 atccctaaat ctggaggtga ttactcctat gtcaaggaca tcttcggagg actggctggg
      961 ttcctgcggc tatggattgc tgtgctggtg atctacccca ccaaccaagc tgtcatcgcc
     1021 ctcaccttct ccaactatgt gctgcagcca ctcttcccta cctgcttccc ccctgagtcc
     1081 ggcctgcgac tcctggctgc catctgcctg ttgctgctca catgggtcaa ctgctccagt
     1141 gtccgatggg ccacccgggt tcaagatatc ttcacggctg ggaagctcct ggccctggct
     1201 ctgatcatca tcatgggtgt tgtgcagata tgcaaaggag aattcttttg gctggagcca
     1261 aagaatgcat ttgagaattt ccaagaacca gacatcggcc tcgtcgctct ggctttcctc
     1321 cagggctcct ttgcctacgg aggctggaac ttccttaatt acgtgacgga ggaacttgtg
     1381 gatccttaca agaaccttcc tagagccatc ttcatttcca tcccactggt cacatttgtg
     1441 tacgtctttg ctaatatcgc ctatgtcact gcaatgtccc cccaggagct gctggcctca
     1501 aatgcagtcg ctgtgacttt tggagagaag ctcctagggg tcatggcctg gatcatgccc
     1561 atttccgtcg ccctgtccac atttggtgga gtcaacggtt ccctcttcac ctcctcccgg
     1621 ctgttctttg ctggagccag agaaggccac cttcccagcg tgttagccat gatccacgtg
     1681 aagcgctgca ccccaatccc agcactgctc ttcacatgcc tctccaccct gctgatgctg
     1741 gtcaccagtg acatgtatac actcatcaac tacgtgggct tcatcaacta cctcttctac
     1801 ggggttacag ttgcaggaca gatagtcctt cgctggaaga agcctgacat cccccgcccc
     1861 atcaagatca gcctgctgtt tcccatcatc tacttgctgt tctgggcctt cctgctgatt
     1921 ttcagcctgt ggtcagagcc agtagtatgc ggcattggcc ttgccatcat gctgacgggt
     1981 gttcctgtat acttcctggg tgtctactgg caacacaaac ccaagtgttt caatgacttc
     2041 attgagtccc taactctagt gagtcagaag atgtgtgtgg tcgtgtatcc ccaggagggg
     2101 gactcgggga ccgaggaaac aattgatgac gtagaggaac aacacaagcc tatcttccag
     2161 cctactcctg tcaaggaccc agactcggag gagcagccct gaagactgcc agcctttaac
     2221 tggccacccc cccttcatcc tttctgccct gtatccctgc ctaggtcccc ccaacacaca
     2281 cacacacaca cacacacaca cactcacaca cacacacaca cacacacaca cacacacaca
     2341 cacacacttc tgttaggcag gggcaagacc tgggtgtcca cagcgagaca ttctaaacaa
     2401 agaccctgac ctttgtaccc aaagaacctg cttccagcac caaggcccat ggtcaaggtc
     2461 agtgcaccgg gacctgcaca caccctaagg tttagaggag agtggaggtg ccattggtac
     2521 cctacagggc ctttcctcct gggcccatgc tctttaggtg cctctaagaa acctgggttc
     2581 actactgttt cttctcccta accctgagcc caggcaaagc ctccactgga aaaaaggtag
     2641 catccccagt gagttagttg gggaagacaa ctcaaattgt caccattaac agtcagctgg
     2701 ggagactcag atgtggcttc attcaccagg gagccaaaag gcagagggct cacttctctt
     2761 tgccttctgc actctgtact accacggctc cactgcccag cctcgacctg agacccacaa
     2821 agttcccttt caggatgcct cttccccaca gagctaagga ccaagaagga ggactgcccc
     2881 cctccccccc agctctcagc caggcttgtg gaatatatag ggaaaggctc agactgcaga
     2941 agccaagccc tgccccaccc ctgcccctgc ctcctgtcct cttgtggtgc caaagctccc
     3001 tgaagcagaa aaacttctta tattcagtct aacagaccta tctctcttaa gggcaaagcg
     3061 actcagctcc tccctgtccc cctgtgtgaa agaaagagca gtagccctct tctcccagca
     3121 gctctgccca ctcacagatc tgcctgccgt cctcctcttt cctcccctgg accttggctc
     3181 aaggaccagg tatgaaggat cccccaagtc cttcaggcct gaagtaagag tcaacccagc
     3241 cttaagtcat ctcccatcca agaactgggt ctaaaacgtt ccccatttct aaccttcaag
     3301 acagacctga tgttaaatcc ttccctggaa ggaagggtgc tttccccctt cccaggggtg
     3361 ctcatccctc accctgggga gaccatgttg gtgaggtagg gggagaatgt tttctgaagg
     3421 ccagttcctc tcccctcctc cagctcaaaa caatctcacc gtcctcctgc catcccattt
     3481 gggacaagat gacaggatgg cactcacttt ggagacctca gaaaagcccc ttccaggaga
     3541 gctgctggtc tgtgctgtac aggaagggtg acagcatctg ccttctcctg gcagcctcca
     3601 cacctgctgt gcttaggaca ggatcctttg atcacaaagc agaaccctaa tcctgttcca
     3661 tctactcaaa ctggaacacc caggggcctt ttcctggtct cccaacttcc cacctagctc
     3721 accctacccc taactggtga gaggctgagt ttgggctgaa ttgtggccct tcagaataca
     3781 gtccaggcag gctgtggggc ctctgggggg gccagggacc acccctctac ccctttcctc
     3841 ccattccttc agcctgcacc tgcccctcct tgaattattg tctctctctc tctttttttt
     3901 tttaagtgga tgccttactt tttggataac tatttttgaa gctggtattt ctatttcttt
     3961 tggatttttt aatgtatggt ggtttggggg cagagctaga accttaatcg tctgtcgctg
     4021 agttcattta ggttttaaat tttattggtt tgttttatgg agtattttct tttctcgtaa
     4081 taaaatttta aatggaaatg aaaaaaaaaa aaaaaaa
//
