LOCUS       NM_017348               3916 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus solute carrier family 6 member 8 (Slc6a8), mRNA.
ACCESSION   NM_017348 XM_346878
VERSION     NM_017348.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Duran-Trio,L., Fernandes-Pires,G., Simicic,D., Grosse,J.,
            Roux-Petronelli,C., Bruce,S.J., Binz,P.A., Sandi,C., Cudalbu,C. and
            Braissant,O.
  TITLE     A new rat model of creatine transporter deficiency reveals
            behavioral disorder and altered brain metabolism
  JOURNAL   Sci Rep 11 (1), 1636 (2021)
   PUBMED   33452333
  REMARK    GeneRIF: A new rat model of creatine transporter deficiency reveals
            behavioral disorder and altered brain metabolism.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Taii,A., Tachikawa,M., Ohta,Y., Hosoya,K.I. and Terasaki,T.
  TITLE     Determination of Intrinsic Creatine Transporter (Slc6a8) Activity
            and Creatine Transport Function of Leukocytes in Rats
  JOURNAL   Biol Pharm Bull 43 (3), 474-479 (2020)
   PUBMED   32115505
REFERENCE   3  (bases 1 to 3916)
  AUTHORS   Yan,J., Zhao,W., Guo,M., Han,X. and Feng,Z.
  TITLE     CXCL12 Regulates the Cholinergic Locus and CHT1 Through Akt
            Signaling Pathway
  JOURNAL   Cell Physiol Biochem 40 (5), 982-992 (2016)
   PUBMED   27941337
  REMARK    GeneRIF: CXCL12 contributes to cholinergic gene expression via Akt
            signaling pathway.
REFERENCE   4  (bases 1 to 3916)
  AUTHORS   DesRoches,C.L., Patel,J., Wang,P., Minassian,B., Salomons,G.S.,
            Marshall,C.R. and Mercimek-Mahmutoglu,S.
  TITLE     Estimated carrier frequency of creatine transporter deficiency in
            females in the general population using functional characterization
            of novel missense variants in the SLC6A8 gene
  JOURNAL   Gene 565 (2), 187-191 (2015)
   PUBMED   25861866
REFERENCE   5  (bases 1 to 3916)
  AUTHORS   Kristofikova,Z., Ripova,D., Hegnerova,K., Sirova,J. and Homola,J.
  TITLE     Protein tau-mediated effects on rat hippocampal choline
            transporters CHT1 and tau-amyloid beta interactions
  JOURNAL   Neurochem Res 38 (9), 1949-1959 (2013)
   PUBMED   23824558
  REMARK    GeneRIF: TP is expressed at high levels in human GBM tumors and
            shRNA knockdown of TP in U87 GBM cells results in a significant
            increase in cellular GCL enzymatic activity
REFERENCE   6  (bases 1 to 3916)
  AUTHORS   Zhao,C.R., Shang,L., Wang,W. and Jacobs,D.O.
  TITLE     Myocellular creatine and creatine transporter serine
            phosphorylation after starvation
  JOURNAL   J Surg Res 105 (1), 10-16 (2002)
   PUBMED   12069495
REFERENCE   7  (bases 1 to 3916)
  AUTHORS   Hahn,K.A., Salomons,G.S., Tackels-Horne,D., Wood,T.C., Taylor,H.A.,
            Schroer,R.J., Lubs,H.A., Jakobs,C., Olson,R.L., Holden,K.R.,
            Stevenson,R.E. and Schwartz,C.E.
  TITLE     X-linked mental retardation with seizures and carrier
            manifestations is caused by a mutation in the creatine-transporter
            gene (SLC6A8) located in Xq28
  JOURNAL   Am J Hum Genet 70 (5), 1349-1356 (2002)
   PUBMED   11898126
REFERENCE   8  (bases 1 to 3916)
  AUTHORS   Lips,K.S., Pfeil,U., Haberberger,R.V. and Kummer,W.
  TITLE     Localisation of the high-affinity choline transporter-1 in the rat
            skeletal motor unit
  JOURNAL   Cell Tissue Res 307 (3), 275-280 (2002)
   PUBMED   11904763
  REMARK    GeneRIF: Data show that choline transporter-1 is restricted to
            motoneurons and absent from the non-neuronal ACh-synthesizing
            elements, and is enriched at neuro-neuronal and neuro-muscular
            synapses.
REFERENCE   9  (bases 1 to 3916)
  AUTHORS   Schloss,P., Mayser,W. and Betz,H.
  TITLE     The putative rat choline transporter CHOT1 transports creatine and
            is highly expressed in neural and muscle-rich tissues
  JOURNAL   Biochem Biophys Res Commun 198 (2), 637-645 (1994)
   PUBMED   8297374
REFERENCE   10 (bases 1 to 3916)
  AUTHORS   Mayser,W., Schloss,P. and Betz,H.
  TITLE     Primary structure and functional expression of a choline
            transporter expressed in the rat nervous system
  JOURNAL   FEBS Lett 305 (1), 31-36 (1992)
   PUBMED   1633856
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000021.1.
            
            On Nov 27, 2020 this sequence version replaced NM_017348.2.
            
            Sequence Note: The sequence X66494.1 is chimeric. Only the Slc6a8
            sequence region was propagated into this RefSeq record.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC168238.1, SRR21887622.1400194.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-849               JAXUCZ010000021.1  156536017-156536865
            850-981             JAXUCZ010000021.1  156538444-156538575
            982-1231            JAXUCZ010000021.1  156539445-156539694
            1232-1364           JAXUCZ010000021.1  156540108-156540240
            1365-1499           JAXUCZ010000021.1  156541362-156541496
            1500-1603           JAXUCZ010000021.1  156541589-156541692
            1604-1728           JAXUCZ010000021.1  156541787-156541911
            1729-1841           JAXUCZ010000021.1  156542448-156542560
            1842-1979           JAXUCZ010000021.1  156542685-156542822
            1980-2082           JAXUCZ010000021.1  156542901-156543003
            2083-2183           JAXUCZ010000021.1  156543090-156543190
            2184-2354           JAXUCZ010000021.1  156543271-156543441
            2355-3916           JAXUCZ010000021.1  156543760-156545321
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq37"
     gene            1..3916
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /note="solute carrier family 6 member 8"
                     /db_xref="GeneID:50690"
                     /db_xref="RGD:619711"
     exon            1..849
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             588..2495
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /note="creatine transporter 1; solute carrier family 6
                     (neurotransmitter transporter, creatine), member 8;
                     choline transporter; solute carrier family 6
                     (neurotransmitter transporter), member 8"
                     /codon_start=1
                     /product="sodium- and chloride-dependent creatine
                     transporter 1"
                     /protein_id="NP_059044.1"
                     /db_xref="GeneID:50690"
                     /db_xref="RGD:619711"
                     /translation="MAKKSAENGIYSVSGDEKKGPLIVSGPDGAPSKGDGPAGLGAPS
                     SRLAVPPRETWTRQMDFIMSCVGFAVGLGNVWRFPYLCYKNGGGVFLIPYVLIALVGG
                     IPIFFLEISLGQFMKAGSINVWNICPLFKGLGYASMVIVFYCNTYYIMVLAWGFYYLV
                     KSFTTTLPWATCGHTWNTPDCVEIFRHEDCANASLANLTCDQLADRRSPVIEFWENKV
                     LRLSTGLEVPGALNWEVTLCLLACWVLVYFCVWKGVKSTGKIVYFTATFPYVVLVVLL
                     VRGVLLPGALDGIIYYLKPDWSKLGSPQVWIDAGTQIFFSYAIGLGALTALGSYNRFN
                     NNCYKDAIILALINSGTSFFAGFVVFSILGFMATEQGVHISKVAESGPGLAFIAYPRA
                     VTLMPVAPLWAALFFFMLLLLGLDSQFVGVEGFITGLLDLLPASYYFRFQREISVALC
                     CALCFVIDLSMVTDGGMYVFQLFDYYSASGTTLLWQAFWECVVVAWVYGADRFMDDIA
                     CMIGYRPCPWMKWCWSFFTPLVCMGIFIFNVVYYKPLVYNNTYVYPWWGEAMGWAFAL
                     SSMLCVPLHLLGCLLRAKGTMAERWQHLTQPIWGLHHLEYRAQDADVRGLTTLTPVSE
                     SSKVVVVESVM"
     exon            850..981
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1231
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1364
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1499
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1603
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1728
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1841
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1842..1979
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2082
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2083..2183
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..2354
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2355..3916
                     /gene="Slc6a8"
                     /gene_synonym="CHOT1; CHT1; CRT; CT1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcggcccgga gagcggctgt agccgccgcc gccgggaagg agggggctag tcgtgcccaa
       61 gaccaccgga gccgccagtg ctagcagagc cccgggcgag caggacagcc gcttggggct
      121 gggccggggc cgcgggccgc aggccccttg ctccggccgc cgcttgcaga cagtgggcgt
      181 ccgatgccat ccgcgacgcg ctagggtgag cctccggcag ggcgagcagt cctcgctgca
      241 gccgccaccg ccgccgccgc cgcccgagcc gcgggcagga gtctcgggag ccgccgccgc
      301 ccggccaggc tccgccgccg cccgcgcgct cccgggcccc cgacacacat gagattcttc
      361 aggctcactt tcaagtgctt cgtggactgc ttctgactgc gccgcccccg ccgtcccgca
      421 cccctcccgc ccgcccgtcg ccccgtcccc cggcccggcc gcccccgagg cccccgggcg
      481 ggcccgcgct ttcggggccc tttctccagg tgccaccgga gccccttgcc cgctcgccac
      541 cctcgagacg ccgcgacccc tggcctggcc tcgcggcccc aggggccatg gcgaagaaga
      601 gcgctgaaaa cggtatctat agcgtgtctg gagacgagaa gaagggtcct ctcatcgtgt
      661 ccgggcccga tggtgccccg tccaagggcg atggccctgc gggcctgggg gcgcccagca
      721 gccgccttgc tgtgccgccg cgagagactt ggacacgcca gatggacttc atcatgtcgt
      781 gcgtgggctt cgccgtgggc ctcggtaacg tgtggcgctt cccctacctg tgctacaaga
      841 acggcggagg tgtgttcctt attccctatg tcctgattgc cctggttgga ggaatcccca
      901 ttttcttcct ggaaatctca ctgggccagt tcatgaaggc cggcagcatc aatgtctgga
      961 acatctgtcc cctattcaaa ggtctgggct atgcctccat ggtgattgtc ttctactgca
     1021 acacttacta catcatggtg ctggcctggg gcttctatta cctggtcaag tcctttacta
     1081 ccactttgcc atgggctacg tgtggccaca cctggaacac tcctgactgt gtagagatct
     1141 ttcgccatga agactgtgcc aatgccagct tggccaacct cacatgtgac cagcttgctg
     1201 accgacggtc ccctgtcatc gagttctggg agaacaaagt cttgaggctc tccacagggc
     1261 tggaggttcc aggagccctc aactgggaag tgaccctgtg cctgctggcc tgctgggtgc
     1321 tggtctactt ctgtgtgtgg aagggggtca agtcaacagg aaagatcgtg tacttcactg
     1381 ctacattccc ctacgtggtc ctcgttgtgc tgctggtgcg aggggtgctg ctgcctggag
     1441 ccctggatgg tatcatctac tatctcaagc ctgactggtc aaagctaggg tcccctcagg
     1501 tatggataga tgctgggacc cagattttct tctcttatgc catcggcctg ggggccctca
     1561 cagccctagg cagctacaat cgcttcaaca acaactgcta caaggatgcc atcatcctgg
     1621 cactcatcaa cagcgggacc agcttctttg ctggctttgt ggtcttctcc atcctgggct
     1681 tcatggccac agagcagggt gtgcatatct ccaaggtggc agaatcaggg cctggtctag
     1741 ccttcattgc ctacccacgg gctgtcacac tgatgcctgt ggccccactc tgggctgcct
     1801 tgttcttctt catgctgctg ctgctcggtc tggacagcca gtttgtaggt gtggagggct
     1861 tcatcactgg gctcctggat ctcctcccgg cctcctacta cttccgtttt caaagggaga
     1921 tctccgtggc cctctgttgt gccctctgct ttgtcatcga tctctccatg gtgactgatg
     1981 gcgggatgta cgtcttccag ctgtttgact actactcagc tagtggcact accctgcttt
     2041 ggcaagcctt ttgggagtgt gtggtggtgg cttgggttta tggagctgac cgcttcatgg
     2101 acgatattgc ctgtatgatt gggtaccgac cttgcccttg gatgaaatgg tgctggtcct
     2161 tcttcacccc actggtctgc atgggcatct tcatcttcaa tgttgtgtac tacaagccac
     2221 tggtctacaa caatacctac gtgtacccat ggtggggtga agctatgggc tgggcctttg
     2281 cactctcttc tatgctctgt gtgcccctcc acctcctggg ctgtctcctc agggcaaaag
     2341 gaaccatggc agagcgatgg cagcacctga cccagcctat ctggggcctc catcacttgg
     2401 aatacagagc tcaggatgca gatgtcaggg gcctgaccac cctgactccc gtgtccgaga
     2461 gcagcaaggt cgtcgtggtg gagagtgtca tgtgacaggc acccccagct cacatcacca
     2521 gctcatcctt tggtagccat agcagcccca gctttagccc ccacccatcc ctcgggggct
     2581 tgcctttcct tgacagtttg gggatctgcc tggggaaagg gaggaagcac catgagtgct
     2641 aacaaaaaca actttttcca tttttaataa aacgccaaaa acatcacaac ccaccaaaaa
     2701 tagatgcctc tcctccccac cccacaacta cgctggtatt ttcgttgctt ccagtcccca
     2761 cctcctttcc ttcttcccag tgcccaggac ccatctccta gccttgccaa gcccatctgc
     2821 ctgcctccca agctctgctc tgtaacacac ctttgcccta gaaccaacag tggcagcttg
     2881 gggaacagga gaggagggaa aataacagta ggacaggagg ggaggcaaga gagggacagt
     2941 ggaaccaagg tgaatatttc agctcaatta gaactcttcc cgcccctatt ctagaagcta
     3001 gagagccagt cagcaatgga accttgtgtt cctgcgccaa ttgccaccaa tattaattgt
     3061 gtgagcttgg gtctgagtgc acgtctgtct ctgtgtgatg tgtgtgtgtg tgtgtgtgtg
     3121 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtaga tctcttatct cttagtgaag
     3181 gtgaatacca gaagtaaaca gagcctctgg gcaaaggagg cttatatttt gcacatttta
     3241 taagaaaaaa aaacttgaga gtgagacttc tgcttgtata tttctaaaaa gcccccacca
     3301 tcccccattt ccactccttt ccttgacatc cccaccttct ctgaccctag ccaaggagtg
     3361 tgaatttata gacttaattt tcataggcaa aacaaaagct tcaggctgtt gaatatgtga
     3421 gtctgtttgt gtggatgttc tgtatggtct ccagccagag actggagtag gaaggtggga
     3481 gcctcaatgg tgtccccaca ctgttccttt gcctctaagt ctgtggggta gaaggctgta
     3541 acattatccc agggaatctg ggtctgctca cctgacatgc tcagacttgc ccacacccag
     3601 ctccctgggc tgggcatgat tgcagggatt tcccatggac atagcccagg gaagggatcc
     3661 tgggtatggc ttccacttcc aagctaaagg ctgatgtgct ttcgacatct caagtcttcc
     3721 gtgtagcagc ttaaacccac atctgtgtcc catcaagcct gaaacagcca agtagccatt
     3781 agaaaggctt tcttcacaca gagctggact aagccaggga ggtggggctg ggtaagggtg
     3841 caagcctttg gggacattct tactgtgcta aaaaagccac tgcaaacata gcaataaaaa
     3901 cacttcattt tccaaa
//
