LOCUS       NM_053996               2721 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus solute carrier family 6 member 7 (Slc6a7), mRNA.
ACCESSION   NM_053996
VERSION     NM_053996.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Anas,M.K., Lee,M.B., Zhou,C., Hammer,M.A., Slow,S., Karmouch,J.,
            Liu,X.J., Broer,S., Lever,M. and Baltz,J.M.
  TITLE     SIT1 is a betaine/proline transporter that is activated in mouse
            eggs after fertilization and functions until the 2-cell stage
  JOURNAL   Development 135 (24), 4123-4130 (2008)
   PUBMED   19029042
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Takemoto,Y. and Semba,R.
  TITLE     Immunohistochemical evidence for the localization of neurons
            containing the putative transmitter L-proline in rat brain
  JOURNAL   Brain Res 1073-1074, 311-315 (2006)
   PUBMED   16458270
  REMARK    GeneRIF: Strong L-proline-like immunoreactivity was confined to
            several groups of neurons in the arcuate nucleus (n) and supraoptic
            n and area postrema and the nerve fiber of brainstem.
REFERENCE   3  (bases 1 to 2721)
  AUTHORS   Galli,A., Jayanthi,L.D., Ramsey,I.S., Miller,J.W., Fremeau,R.T. Jr.
            and DeFelice,L.J.
  TITLE     L-proline and L-pipecolate induce enkephalin-sensitive currents in
            human embryonic kidney 293 cells transfected with the high-affinity
            mammalian brain L-proline transporter
  JOURNAL   J Neurosci 19 (15), 6290-6297 (1999)
   PUBMED   10414958
REFERENCE   4  (bases 1 to 2721)
  AUTHORS   Renick,S.E., Kleven,D.T., Chan,J., Stenius,K., Milner,T.A.,
            Pickel,V.M. and Fremeau,R.T. Jr.
  TITLE     The mammalian brain high-affinity L-proline transporter is enriched
            preferentially in synaptic vesicles in a subpopulation of
            excitatory nerve terminals in rat forebrain
  JOURNAL   J Neurosci 19 (1), 21-33 (1999)
   PUBMED   9870934
REFERENCE   5  (bases 1 to 2721)
  AUTHORS   Fremeau,R.T. Jr., Caron,M.G. and Blakely,R.D.
  TITLE     Molecular cloning and expression of a high affinity L-proline
            transporter expressed in putative glutamatergic pathways of rat
            brain
  JOURNAL   Neuron 8 (5), 915-926 (1992)
   PUBMED   1350201
REFERENCE   6  (bases 1 to 2721)
  AUTHORS   Nickolson,V.J.
  TITLE     'On' and 'off' responses of K+-induced synaptosomal proline
            release: involvement of the sodium pump
  JOURNAL   J Neurochem 38 (1), 289-292 (1982)
   PUBMED   6286882
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            M88111.1 and JAXUCZ010000018.1.
            
            On Mar 15, 2007 this sequence version replaced NM_053996.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M88111.1, SRR26643298.74232.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1191              M88111.1           1-1191
            1192-1193           JAXUCZ010000018.1  56725230-56725231   c
            1194-2059           M88111.1           1194-2059
            2060-2061           JAXUCZ010000018.1  56719509-56719510   c
            2062-2721           M88111.1           2061-2720
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18q12.1"
     gene            1..2721
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /note="solute carrier family 6 member 7"
                     /db_xref="GeneID:117100"
                     /db_xref="RGD:620928"
     exon            1..117
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..1998
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /note="L-proline transporter; solute carrier family 6
                     (neurotransmitter transporter, L-proline), member 7;
                     solute carrier family 6 (neurotransmitter transporter),
                     member 7; sodium-dependent proline transporter-like"
                     /codon_start=1
                     /product="sodium-dependent proline transporter"
                     /protein_id="NP_446448.2"
                     /db_xref="GeneID:117100"
                     /db_xref="RGD:620928"
                     /translation="MKKLQEAHLRKPVTPDLLMTPSDQGDVDLDVDFAADRGNWTGKL
                     DFLLSCIGYCVGLGNVWRFPYRAYTNGGGAFLVPYFLMLAICGIPLFFLELSLGQFSS
                     LGPLAVWKISPLFKGAGAAMLLIVGLVAIYYNMIIAYVLFYLFASLTSNLPWEHCGNW
                     WNTERCLEHRGPKDGNGALPLNLSSTVSPSEEYWSRYVLHIQGSQGIGRPGEIRWNLC
                     LCLLLAWVIVFLCILKGVKSSGKVVYFTATFPYLILLMLLVRGVTLPGAWKGIQFYLT
                     PQFHHLLSSKVWIEAALQIFYSLGVGFGGLLTFASYNTFHQNIYRDTFIVTLGNAITS
                     ILAGFAIFSVLGYMSQELGVPVDQVAKAGPGLAFVVYPQAMTMLPLSPFWSFLFFFML
                     LTLGLDSQFAFLETIVTAVTDEFPYYLRPKKAVFSGLICVAMYLMGLILTTDGGMYWL
                     VLLDDYSASFGLMVVVITTCLAVTRVYGIQRFCRDIHMMLGFKPGLYFRACWLFLSPA
                     TLLALLVYSIVKYQPSEYGSYRFPAWAELLGILMGLLSCLMIPAGMLVAVLREEGSLW
                     ERLQQASRPAIDWGPSLEENRTGMYVATLAGSQSPKPLMVHMRKYGGITSFENTAIEV
                     DREIAEEEEESMM"
     exon            118..301
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            302..433
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            434..668
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            669..807
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            808..942
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1046
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1171
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1284
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1416
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1516
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1617
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..1785
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..2721
                     /gene="Slc6a7"
                     /gene_synonym="Prot"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaaggcgcag agataggacc agtgctcggc gcccgcttgg ctggctgact gcgctcttgc
       61 aagcaccggt gccagctctc caagatgaag aagctccagg aagctcacct ccgcaagcct
      121 gtcaccccag acctgctgat gactcccagt gaccagggtg atgtggacct ggatgtagac
      181 tttgcagcag acagaggcaa ctggacgggc aagctggact tcttgctgtc ctgcatcggc
      241 tactgtgtgg gcttgggaaa tgtctggcgg tttccctatc gagcctacac caatggaggc
      301 ggagccttcc tcgtgcccta cttcctcatg ctggccatct gtggcatccc cctcttcttt
      361 cttgagctct ccctgggcca gttctccagc ctgggacccc tggctgtctg gaaaatcagc
      421 cccctcttca aaggtgcggg tgcagccatg ctgctcatcg tgggcctggt ggccatctac
      481 tacaacatga tcatcgccta cgtcctcttc tacctcttcg cctccctcac cagcaacctg
      541 ccctgggagc attgcggcaa ctggtggaac acagaacgct gcctggagca cagaggcccc
      601 aaggatggca acggggcact gcctcttaac ctcagcagca ctgtcagccc cagtgaggag
      661 tactggagcc gatatgtcct gcacattcag ggcagccagg gcatcggccg acccggggag
      721 attcgctgga acctctgcct ctgcctgctg ctggcctggg tcatcgtgtt tctctgtatc
      781 ctgaaggggg tgaagtcctc gggcaaggtg gtgtatttca cagccacctt tccctacctc
      841 atcctgctca tgctcctggt tcgaggagtg acccttcccg gggcctggaa gggcatccag
      901 ttctatctca ccccccaatt ccaccacctg ctgtcttcca aggtgtggat tgaagctgct
      961 cttcagatct tctactctct aggagtgggt tttgggggtc ttctcacctt tgcctcctac
     1021 aacacattcc accagaacat ctacagagac accttcattg tcactctggg caatgccatc
     1081 accagcatcc tggctggttt tgctatcttc tcggtgctgg gctacatgtc gcaggagctg
     1141 ggtgtgcctg tggaccaagt ggccaaagca ggccctggcc tggcctttgt tgtctaccca
     1201 caggccatga ctatgttgcc tctgtcaccc ttctggtcct tcctcttctt cttcatgctt
     1261 ctgactcttg gcctcgatag ccagtttgcc tttctggaaa ccatagtgac tgcagtgacc
     1321 gatgagttcc catactacct acggcccaag aaggcagtgt tctcaggcct catctgtgta
     1381 gccatgtacc tgatgggact gatcctcacc accgatgggg ggatgtactg gctggtcctt
     1441 ctggatgact acagcgccag cttcggactc atggtggtgg tgattaccac atgcctcgct
     1501 gtcacccggg tatacggcat ccagcggttt tgtcgtgaca tccacatgat gctgggcttc
     1561 aagccaggac tctacttcag ggcctgctgg ctgtttttgt ctccggccac actcttggcc
     1621 ttgctggtgt acagtatcgt caagtaccag ccctcggaat acggtagcta tcgcttcccc
     1681 gcctgggccg agctgctagg catcctgatg ggcctgctct cctgcctcat gatcccagct
     1741 ggcatgctgg tagctgtgct tcgagaggag ggctcgctct gggagcgact tcagcaagcc
     1801 agccgtcctg ctatagactg gggcccatca ctggaagaga accggacggg catgtatgtg
     1861 gccaccctgg ctgggagcca gtcaccaaaa ccactgatgg tacacatgcg aaaatatggg
     1921 ggcatcacca gcttcgagaa tacagccatt gaggtggacc gtgagatcgc agaggaggaa
     1981 gaggagtcca tgatgtgaga ccagacacct ccaaacagga gggctggtcg gggcctcccc
     2041 gtctgtccct tccttggccc acaggggata gctttgtctg ttgggattct gacaggcaat
     2101 gggaggttgc catggcaacg acagtcccca gcctaagtcc ctctttgtgg cctctacatc
     2161 tcctggaacc tctagatgga catacatata ctaggtaacc cattcaaagc tgaaacgatt
     2221 cagctcagcc ctcagtttgt gagggggtgc gttgaagcca gggagagaag agctggacca
     2281 aggtgacatg cccaagagga cttgttccca agcctcctcc agccagtcaa ctccctttcc
     2341 cttgggggag caagcaccat atctgacatc tttgttcaga cactttgaca agatacattt
     2401 ccatacaagc caactttaaa ccccaggttc agggtagcga gaacctggag agccccaagg
     2461 ccctggatat agatagaatg gcagcgacca aattgggtag aaaagtcttt gtgggttcct
     2521 gtgttaaggc cagttttccc agaagaagtg ggagctttag ggctgagagg tgtgaaagat
     2581 tgcagaagag gctagaggga aggtttgcgg gtcagagctg cccctgagcc aggaggaggc
     2641 ccaacctgcc agagcagaga ccaggagggg ctggagattg tggtgctccc gggttatgag
     2701 agggaataaa gactcgcagg g
//
