LOCUS       NP_037166                630 aa            linear   ROD 24-SEP-2024
DEFINITION  sodium-dependent serotonin transporter [Rattus norvegicus].
ACCESSION   NP_037166 XP_001080660 XP_001080667
VERSION     NP_037166.2
DBSOURCE    REFSEQ: accession NM_013034.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 630)
  AUTHORS   Boillot,M., Ter Horst,J., Lopez,J.R., Di Fazio,I., Steens,I.L.M.,
            Cohen,M.X. and Homberg,J.R.
  TITLE     Serotonin transporter knockout in rats reduces beta- and gamma-band
            functional connectivity between the orbitofrontal cortex and
            amygdala during auditory discrimination
  JOURNAL   Cereb Cortex 34 (8) (2024)
   PUBMED   39128940
  REMARK    GeneRIF: Serotonin transporter knockout in rats reduces beta- and
            gamma-band functional connectivity between the orbitofrontal cortex
            and amygdala during auditory discrimination.
REFERENCE   2  (residues 1 to 630)
  AUTHORS   Sun,M., Brivio,P., Shan,L., Docq,S., Heltzel,L.C.M.W.,
            Smits,C.A.J., Middelman,A., Vrooman,R., Spoelder,M.,
            Verheij,M.M.M., Buitelaar,J.K., Boillot,M., Calabrese,F.,
            Homberg,J.R. and Hanswijk,S.I.
  TITLE     Offspring's own serotonin transporter genotype, independently from
            the maternal one, increases anxiety- and depression-like behavior
            and alters neuroplasticity markers in rats
  JOURNAL   J Affect Disord 350, 89-101 (2024)
   PUBMED   38220097
  REMARK    GeneRIF: Offspring's own serotonin transporter genotype,
            independently from the maternal one, increases anxiety- and
            depression-like behavior and alters neuroplasticity markers in
            rats.
REFERENCE   3  (residues 1 to 630)
  AUTHORS   Ngoupaye,G.T., Mokgokong,M., Madlala,T. and Mabandla,M.V.
  TITLE     Alteration of the alpha5 GABA receptor and 5HTT lead to cognitive
            deficits associated with major depressive-like behaviors in a
            14-day combined stress rat model
  JOURNAL   Int J Neurosci 133 (9), 959-976 (2023)
   PUBMED   34937496
  REMARK    GeneRIF: Alteration of the alpha5 GABA receptor and 5HTT lead to
            cognitive deficits associated with major depressive-like behaviors
            in a 14-day combined stress rat model.
REFERENCE   4  (residues 1 to 630)
  AUTHORS   Yildirim,H., Efe Daskan,B., Aksoz,E., Sen,F. and Celebi,M.
  TITLE     Regional expression differences of SERT and TSPAN8 in hippocampus,
            cerebellum and cortex of wild-type young, adult and middle-aged
            rats
  JOURNAL   Gene 885, 147706 (2023)
   PUBMED   37572802
  REMARK    GeneRIF: Regional expression differences of SERT and TSPAN8 in
            hippocampus, cerebellum and cortex of wild-type young, adult and
            middle-aged rats.
REFERENCE   5  (residues 1 to 630)
  AUTHORS   Thorne,B.N., Ellenbroek,B.A. and Day,D.J.
  TITLE     The serotonin reuptake transporter modulates mitochondrial copy
            number and mitochondrial respiratory complex gene expression in the
            frontal cortex and cerebellum in a sexually dimorphic manner
  JOURNAL   J Neurosci Res 100 (3), 869-879 (2022)
   PUBMED   35043462
  REMARK    GeneRIF: The serotonin reuptake transporter modulates mitochondrial
            copy number and mitochondrial respiratory complex gene expression
            in the frontal cortex and cerebellum in a sexually dimorphic
            manner.
REFERENCE   6  (residues 1 to 630)
  AUTHORS   Ramamoorthy,J.D., Ramamoorthy,S., Papapetropoulos,A.,
            Catravas,J.D., Leibach,F.H. and Ganapathy,V.
  TITLE     Cyclic AMP-independent up-regulation of the human serotonin
            transporter by staurosporine in choriocarcinoma cells
  JOURNAL   J Biol Chem 270 (29), 17189-17195 (1995)
   PUBMED   7615516
REFERENCE   7  (residues 1 to 630)
  AUTHORS   Ramamoorthy,S., Bauman,A.L., Moore,K.R., Han,H., Yang-Feng,T.,
            Chang,A.S., Ganapathy,V. and Blakely,R.D.
  TITLE     Antidepressant- and cocaine-sensitive human serotonin transporter:
            molecular cloning, expression, and chromosomal localization
  JOURNAL   Proc Natl Acad Sci U S A 90 (6), 2542-2546 (1993)
   PUBMED   7681602
REFERENCE   8  (residues 1 to 630)
  AUTHORS   Mayser,W., Betz,H. and Schloss,P.
  TITLE     Isolation of cDNAs encoding a novel member of the neurotransmitter
            transporter gene family
  JOURNAL   FEBS Lett 295 (1-3), 203-206 (1991)
   PUBMED   1765155
REFERENCE   9  (residues 1 to 630)
  AUTHORS   Blakely,R.D., Berson,H.E., Fremeau,R.T. Jr., Caron,M.G., Peek,M.M.,
            Prince,H.K. and Bradley,C.C.
  TITLE     Cloning and expression of a functional serotonin transporter from
            rat brain
  JOURNAL   Nature 354 (6348), 66-70 (1991)
   PUBMED   1944572
REFERENCE   10 (residues 1 to 630)
  AUTHORS   Hoffman,B.J., Mezey,E. and Brownstein,M.J.
  TITLE     Cloning of a serotonin transporter affected by antidepressants
  JOURNAL   Science 254 (5031), 579-580 (1991)
   PUBMED   1948036
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            M79450.1, CB736426.1, JAXUCZ010000010.1, BF548147.1 and AI547408.1.
            
            On or before Jun 25, 2006 this sequence version replaced
            XP_001080667.1, XP_001080660.1, NP_037166.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y11024.1, X63995.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN06621351, SAMN06680412
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q24"
     Protein         1..630
                     /product="sodium-dependent serotonin transporter"
                     /note="Solute carrier family 6 (neurotransmitter
                     transporter serotonin) member 4 (5-hydroxytryptamine
                     (serotonin) transporter); serotonin transporter; 5HTT; 5HT
                     transporter; solute carrier family 6 (neurotransmitter
                     transporter), member 4; solute carrier family 6
                     (neurotransmitter transporter, serotonin), member 4"
                     /calculated_mol_wt=70041
     Region          23..60
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Region          24..64
                     /region_name="5HT_transport_N"
                     /note="Serotonin (5-HT) neurotransmitter transporter,
                     N-terminus; pfam03491"
                     /db_xref="CDD:397524"
     Site            47
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:P31645; propagated from
                     UniProtKB/Swiss-Prot (P31652.1)"
     Region          79..615
                     /region_name="SLC6sbd_SERT"
                     /note="Na(+)- and Cl(-)-dependent serotonin transporter
                     SERT; solute-binding domain; cd11513"
                     /db_xref="CDD:271399"
     Site            88..112
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Site            order(94,97,434,437..438)
                     /site_type="other"
                     /note="Na binding site 2 [ion binding]"
                     /db_xref="CDD:271399"
     Site            order(95..96,98..101,176,335..336,341,343,438,441..442)
                     /site_type="other"
                     /note="putative substrate binding site 1 [chemical
                     binding]"
                     /db_xref="CDD:271399"
     Site            order(96,101,336,368)
                     /site_type="other"
                     /note="Na binding site 1 [ion binding]"
                     /db_xref="CDD:271399"
     Site            order(103..104,175,179,182,403,407)
                     /site_type="other"
                     /note="putative substrate binding site 2 [chemical
                     binding]"
                     /db_xref="CDD:271399"
     Site            116..135
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Site            142
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:P31645; propagated from
                     UniProtKB/Swiss-Prot (P31652.1)"
     Site            161..186
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Site            208
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000250|UniProtKB:P31645; propagated from
                     UniProtKB/Swiss-Prot (P31652.1)"
     Site            217
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000250|UniProtKB:P31645; propagated from
                     UniProtKB/Swiss-Prot (P31652.1)"
     Site            253..271
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Site            276
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000269|PubMed:17310063; propagated from
                     UniProtKB/Swiss-Prot (P31652.1)"
     Site            278..297
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Site            325..347
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Site            361..380
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Site            422..443
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Site            464..483
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Site            495..516
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Site            539..558
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Site            575..595
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     Region          616..624
                     /region_name="Interaction with RAB4A.
                     /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (P31652.1)"
     CDS             1..630
                     /gene="Slc6a4"
                     /gene_synonym="SERT"
                     /coded_by="NM_013034.4:108..2000"
                     /db_xref="GeneID:25553"
                     /db_xref="RGD:3714"
ORIGIN      
        1 mettplnsqk vlseckdred cqengvlqkg vpttadraep sqisngysav pstsagdeas
       61 hsipaatttl vaeirqgere twgkkmdfll svigyavdlg niwrfpyicy qngggafllp
      121 ytimaifggi plfymelalg qyhrngcisi wrkicpifkg igyaiciiaf yiasyyntii
      181 awalyyliss ltdrlpwtsc tnswntgnct nyfaqdnitw tlhstspaee fylrhvlqih
      241 qskglqdlgt iswqltlciv liftviyfsi wkgvktsgkv vwvtatfpyi vlsvllvrga
      301 tlpgawrgvv fylkpnwqkl letgvwvdaa aqiffslgpg fgvllafasy nkfnnncyqd
      361 alvtsvvncm tsfvsgfvif tvlgymaemr nedvsevakd agpsllfity aeaianmpas
      421 tffaiifflm litlgldstf aglegvitav ldefphiwak rrewfvlivv itcvlgsllt
      481 ltsggayvvt lleeyatgpa vltvalieav avswfygitq fcsdvkemlg fspgwfwric
      541 wvaisplfll fiicsflmsp pqlrlfqyny phwsivlgyc igmssvicip tyiiyrlist
      601 pgtlkeriik sitpetptei pcgdirmnav
//
