LOCUS       NM_017335               2561 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Rattus norvegicus solute carrier family 6 member 12 (Slc6a12),
            mRNA.
ACCESSION   NM_017335
VERSION     NM_017335.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Zhang,J., Da,Y., Lu,J., Sun,Y., Wang,H., Wang,K., Wang,W., Qin,L.
            and Jia,J.
  TITLE     Changes in vesicular glutamate transporter 2 (Vglut2) and vesicular
            GABA transporter 1 (Vgat1) in the orofacial pain and temperature
            perception pathway under low estrogen conditions
  JOURNAL   Neuro Endocrinol Lett 43 (2), 88-98 (2022)
   PUBMED   35933616
  REMARK    GeneRIF: Changes in vesicular glutamate transporter 2 (Vglut2) and
            vesicular GABA transporter 1 (Vgat1) in the orofacial pain and
            temperature perception pathway under low estrogen conditions.
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Santos,M.S., Park,C.K., Foss,S.M., Li,H. and Voglmaier,S.M.
  TITLE     Sorting of the vesicular GABA transporter to functional vesicle
            pools by an atypical dileucine-like motif
  JOURNAL   J Neurosci 33 (26), 10634-10646 (2013)
   PUBMED   23804087
  REMARK    GeneRIF: we report the identification of a unique amino acid motif
            in the vesicular GABA transporter (VGAT) that controls its synaptic
            localization and activity-dependent recycling
REFERENCE   3  (bases 1 to 2561)
  AUTHORS   Kersante,F., Rowley,S.C., Pavlov,I., Gutierrez-Mecinas,M.,
            Semyanov,A., Reul,J.M., Walker,M.C. and Linthorst,A.C.
  TITLE     A functional role for both -aminobutyric acid (GABA) transporter-1
            and GABA transporter-3 in the modulation of extracellular GABA and
            GABAergic tonic conductances in the rat hippocampus
  JOURNAL   J Physiol 591 (10), 2429-2441 (2013)
   PUBMED   23381899
  REMARK    GeneRIF: GAT-1, which is predominantly presynaptically located, is
            the major GABA transporter in the hippocampus under baseline,
            quiescent conditions.
REFERENCE   4  (bases 1 to 2561)
  AUTHORS   Pirttimaki,T., Parri,H.R. and Crunelli,V.
  TITLE     Astrocytic GABA transporter GAT-1 dysfunction in experimental
            absence seizures
  JOURNAL   J Physiol 591 (4), 823-833 (2013)
   PUBMED   23090943
  REMARK    GeneRIF: The results demonstrate a malfunction of GAT-1 in thalamic
            astrocytes of a well-established genetic model of absence epilepsy,
            and indicate that this abnormality is not a reflection of a general
            pathology affecting all astrocytic functions.
REFERENCE   5  (bases 1 to 2561)
  AUTHORS   Jin,X.T., Pare,J.F. and Smith,Y.
  TITLE     GABA transporter subtype 1 and GABA transporter subtype 3 modulate
            glutamatergic transmission via activation of presynaptic GABA(B)
            receptors in the rat globus pallidus
  JOURNAL   Eur J Neurosci 36 (4), 2482-2492 (2012)
   PUBMED   22616751
  REMARK    GeneRIF: This study provides evidence that the GAT-1-mediated
            regulation of extracellular GABA can modulate glutamatergic
            transmission at the subthalamopallidal synapse in the rat globus
            pallidus.
REFERENCE   6  (bases 1 to 2561)
  AUTHORS   Farhan,H., Korkhov,V.M., Paulitschke,V., Dorostkar,M.M.,
            Scholze,P., Kudlacek,O., Freissmuth,M. and Sitte,H.H.
  TITLE     Two discontinuous segments in the carboxyl terminus are required
            for membrane targeting of the rat gamma-aminobutyric acid
            transporter-1 (GAT1)
  JOURNAL   J Biol Chem 279 (27), 28553-28563 (2004)
   PUBMED   15073174
  REMARK    GeneRIF: two discontinuous segments in the carboxyl terminus are
            required for membrane targeting of the rat GAT1
REFERENCE   7  (bases 1 to 2561)
  AUTHORS   Quick,M.W., Hu,J., Wang,D. and Zhang,H.Y.
  TITLE     Regulation of a gamma-aminobutyric acid transporter by reciprocal
            tyrosine and serine phosphorylation
  JOURNAL   J Biol Chem 279 (16), 15961-15967 (2004)
   PUBMED   14761965
  REMARK    GeneRIF: GABA transporter can exist in either of two mutually
            exclusive phosphorylation states and that the relative abundance of
            these states determines in part the relative subcellular
            distribution of the transporter
REFERENCE   8  (bases 1 to 2561)
  AUTHORS   Horii,A., Kitahara,T., Smith,P.F., Darlington,C.L., Masumura,C. and
            Kubo,T.
  TITLE     Effects of unilateral labyrinthectomy on GAD, GAT1 and GABA
            receptor gene expression in the rat vestibular nucleus
  JOURNAL   Neuroreport 14 (18), 2359-2363 (2003)
   PUBMED   14663191
  REMARK    GeneRIF: GAT1 mRNA expression is initially up-regulated in the
            ipsilateral vestibular nucleus and then undergoes a bilateral
            increase 50 h after labyrinthectomy.
REFERENCE   9  (bases 1 to 2561)
  AUTHORS   Minelli,A., Alonso-Nanclares,L., Edwards,R.H., DeFelipe,J. and
            Conti,F.
  TITLE     Postnatal development of the vesicular GABA transporter in rat
            cerebral cortex
  JOURNAL   Neuroscience 117 (2), 337-346 (2003)
   PUBMED   12614674
  REMARK    GeneRIF: During neocortical development the expression of GABA
            transporter in the cerebral cortex slightly precedes the complete
            maturation of inhibitory synaptogenesis and suggests that it may
            contribute to the formation of neocortical GABAergic circuitry.
REFERENCE   10 (bases 1 to 2561)
  AUTHORS   Burnham,C.E., Buerk,B., Schmidt,C. and Bucuvalas,J.C.
  TITLE     A liver-specific isoform of the betaine/GABA transporter in the
            rat: cDNA sequence and organ distribution
  JOURNAL   Biochim Biophys Acta 1284 (1), 4-8 (1996)
   PUBMED   8865807
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from U28927.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U28927.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     gene            1..2561
                     /gene="Slc6a12"
                     /gene_synonym="BGT1; Gat1; RNU28927; VGAT"
                     /note="solute carrier family 6 member 12"
                     /db_xref="GeneID:50676"
                     /db_xref="RGD:620255"
     CDS             305..2191
                     /gene="Slc6a12"
                     /gene_synonym="BGT1; Gat1; RNU28927; VGAT"
                     /note="solute carrier family 6 (neurotransmitter
                     transporter), member 12; solute carrier family 6
                     (neurotransmitter transporter, betaine/GABA), member 12;
                     Na(+)/Cl(-) betaine/GABA transporter; vesicular GABA
                     transporter; GABA transporter 1; Betaine/GABA transporter
                     1"
                     /codon_start=1
                     /product="sodium- and chloride-dependent betaine
                     transporter"
                     /protein_id="NP_059031.1"
                     /db_xref="GeneID:50676"
                     /db_xref="RGD:620255"
                     /translation="MSSGAWEERVSLSSMDRKVTVHEDGCPVVSWVPEEGEMMDQKDK
                     DQVKDRGQWTNKMEFVLSVAGEIIGLGNVWRFPYLCYKNGGGAFFIPYFIFFFSCGIP
                     VFFLEVALGQYSSQGSVTAWRKICPLLQGIGMASVVIESYLNIYYIIILAWALFYLFS
                     SFTWELPWTTCTNSWNTEHCVDFLNYSSTRAASYSENFTSPVMEFWERRVLGITSGIH
                     DLGSLRWELALCLLLAWIICYFCIWKGVKSTGKVVYFTATFPYLMLIILLIRGVTLPG
                     AYQGIVFYLKPDLLRLKDPQVWMDAGTQIFFSFAICQGCLTALGSYNKYHNNCYRDSI
                     ALCFLNSATSFVAGFVVFSILGFMAQEQGVPISEVAESGPGLAFIAFPKAVTMMPLSQ
                     LWSCLFFLMLLFLGLDSQFVCMECLVTASMDMFPQQLRKRGRRELLILAVAIVCYLMG
                     LLLVTEGGMYIFQLFDYYASSGICLLFLSLFEVICIGWVYGADRFYDNVEDMIGYRPW
                     PLVKISWLFLTPGLCLATFFFSLSKYTPLKYNNVYIYPSWGYSIGWLLAFSSMACVPL
                     FIIITLLKTQGSFKKRLQRLITPDPSLPQPGRRSPQDGSSAQNCSTSPVKQELIAWEK
                     ETHL"
ORIGIN      
        1 agtgtactgg agatctggac agaagggcca gctggagtgg tggccggctg tagagatcaa
       61 aagggatctg agctataatg gggttctaat cacagctcta cccctgggtg gtgttgtgca
      121 agtctctgac ccctgaaagc ctgctctgtc tgttgctttg tctgccgaag actgtgtgca
      181 agtctctgac ccctgaaagc ctgctctgtc tgttgctttg tctgccgaag acttggcaag
      241 ctaggatctg ctccctgttg gctcagccca taactctgag agcagactgt cactttagat
      301 agaaatgtca tcgggagctt gggaagaaag ggtctccctc tccagtatgg acagaaaggt
      361 tacagtccat gaagatgggt gtcctgtggt ctcttgggtc cctgaggagg gagagatgat
      421 ggaccagaaa gacaaggacc aggtgaagga tcgaggccaa tggaccaaca agatggagtt
      481 cgtactgtca gtggcgggag agatcattgg cttaggcaac gtctggaggt ttccctatct
      541 ctgctacaag aacgggggag gagccttctt catcccctac ttcatcttct tcttcagctg
      601 tggtatccca gtgttcttcc tggaggtggc actgggtcag tatagcagcc aggggagtgt
      661 tactgcttgg aggaagatct gtccccttct ccaaggcatt ggcatggcat ctgtggtcat
      721 cgagtcttat ttgaacatct actacatcat catccttgcc tgggctctct tctacctgtt
      781 cagctccttc acctgggagc ttccctggac aacctgcacc aactcctgga acacagaaca
      841 ttgcgtggac tttctgaact actcatcgac cagagccgca agctactctg agaacttcac
      901 ctcaccagtc atggaattct gggagagacg ggttttgggt attacatcag gcatccatga
      961 cctggggtcc ctgcgctggg agctggccct gtgcctcctg ctcgcctgga tcatctgcta
     1021 cttctgcatc tggaagggag tcaagtccac gggcaaggtt gtttacttca cagccacttt
     1081 tccctacctg atgctgatta ttcttctgat ccggggtgtt acccttcctg gagcctacca
     1141 gggcatcgtc ttctacctga agccagactt gctccgcctc aaggaccccc aggtgtggat
     1201 ggatgcgggc acccagatct tcttttcctt tgccatctgc caggggtgcc tcacggccct
     1261 gggcagctac aacaagtacc acaacaactg ctacagggac tccatcgccc tctgcttcct
     1321 gaacagtgcc accagcttcg tggctggttt tgttgtcttc tccatcctgg gcttcatggc
     1381 tcaggagcag ggcgtgccca tttctgaagt ggctgagtca ggtcctggac tggctttcat
     1441 tgccttcccc aaagcagtga cgatgatgcc tttgtcccag ctgtggtcct gcctcttctt
     1501 cctcatgctc ctatttctag ggctggacag ccagttcgtc tgtatggagt gcctggtcac
     1561 tgcttccatg gatatgtttc ctcagcagct ccgaaagaga ggccggcgcg agctgctgat
     1621 cctcgcagtt gctatcgtgt gctacttgat ggggctcctt ctggtcactg agggtgggat
     1681 gtacattttc cagctctttg actactacgc ctccagcgga atatgcctac tcttcctctc
     1741 cttgtttgag gtcatctgca ttggctgggt gtacggggcg gatcgtttct atgacaacgt
     1801 ggaggacatg attggctatc ggccatggcc cttggtgaag atctcttggc tcttcctgac
     1861 ccctggtctt tgcctggcca ccttcttctt ctccttgagc aagtatacac ctctcaaata
     1921 caacaatgtc tacatatacc cttcttgggg atactccatt ggatggctct tggctttttc
     1981 ctccatggcc tgtgtaccgc tctttattat catcaccctc ctgaagaccc agggctcctt
     2041 caagaagcgc cttcaaagac tcatcacgcc agatcccagt ctgccccagc cagggaggcg
     2101 ttcacctcag gatggctcct ctgctcaaaa ctgttcgacc tctccagtca agcaagaact
     2161 cattgcctgg gagaaggaga cgcatttgta gggagtgcca gaggccaggt aactccagag
     2221 ccaggaacct ggtcaaggct agctcctccc agggtgaacc tctcttccac cctgttgaga
     2281 cctcccaggt ctctggacac ccctgctggt cttcgtttgg ctgggacagc ctgtcaccta
     2341 ccagtccttt ccagatgtgc tccaggaatc tgtttgccaa gacttttgaa ctcaatgtcc
     2401 tgactttttg gatttaaaaa aatgaagaat cagaaaggca aagtgatatt ttcaaggtca
     2461 tgcagccttg cagagcccaa gaccttcctt ggacttgctg ctctctgctg aggagaacct
     2521 ccagcctcac ccctgcagca gttaacaagc ctgtgtggat c
//
