LOCUS       NM_024371               4045 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus solute carrier family 6 member 1 (Slc6a1), mRNA.
ACCESSION   NM_024371
VERSION     NM_024371.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Dayan-Alon,O. and Kanner,B.I.
  TITLE     Internal gate mutants of the GABA transporter GAT1 are capable of
            substrate exchange
  JOURNAL   Neuropharmacology 161, 107534 (2019)
   PUBMED   30790582
  REMARK    GeneRIF: perturbation of the internal gate of GAT1 selectively
            impairs the gating mechanism of the empty transporter
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Kaewman,P., Nudmamud-Thanoi,S. and Thanoi,S.
  TITLE     GABAergic Alterations in the Rat Testis after Methamphetamine
            Exposure
  JOURNAL   Int J Med Sci 15 (12), 1349-1354 (2018)
   PUBMED   30275762
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4045)
  AUTHORS   Enoch,M.A., Hodgkinson,C.A., Shen,P.H., Gorodetsky,E.,
            Marietta,C.A., Roy,A. and Goldman,D.
  TITLE     GABBR1 and SLC6A1, Two Genes Involved in Modulation of GABA
            Synaptic Transmission, Influence Risk for Alcoholism: Results from
            Three Ethnically Diverse Populations
  JOURNAL   Alcohol Clin Exp Res 40 (1), 93-101 (2016)
   PUBMED   26727527
REFERENCE   4  (bases 1 to 4045)
  AUTHORS   Moldavan,M., Cravetchi,O., Williams,M., Irwin,R.P., Aicher,S.A. and
            Allen,C.N.
  TITLE     Localization and expression of GABA transporters in the
            suprachiasmatic nucleus
  JOURNAL   Eur J Neurosci 42 (12), 3018-3032 (2015)
   PUBMED   26390912
  REMARK    GeneRIF: Study demonstrated that GAT1 and GAT3 were expressed in
            glial cells but not at detectable levels in suprachiasmatic
            hypothalamic nucleus neurons
REFERENCE   5  (bases 1 to 4045)
  AUTHORS   Savtchenko,L., Megalogeni,M., Rusakov,D.A., Walker,M.C. and
            Pavlov,I.
  TITLE     Synaptic GABA release prevents GABA transporter type-1 reversal
            during excessive network activity
  JOURNAL   Nat Commun 6, 6597 (2015)
   PUBMED   25798861
  REMARK    GeneRIF: Epileptiform activity is associated with increased
            synaptic GABA release and is not accompanied by GAT-1 reversal.
            Sustained efflux of GABA through GAT-1 is unlikely to occur during
            physiological or pathological network activity.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4045)
  AUTHORS   Schmid,J.A., Scholze,P., Kudlacek,O., Freissmuth,M., Singer,E.A.
            and Sitte,H.H.
  TITLE     Oligomerization of the human serotonin transporter and of the rat
            GABA transporter 1 visualized by fluorescence resonance energy
            transfer microscopy in living cells
  JOURNAL   J Biol Chem 276 (6), 3805-3810 (2001)
   PUBMED   11071889
REFERENCE   7  (bases 1 to 4045)
  AUTHORS   Loo,D.D., Eskandari,S., Boorer,K.J., Sarkar,H.K. and Wright,E.M.
  TITLE     Role of Cl- in electrogenic Na+-coupled cotransporters GAT1 and
            SGLT1
  JOURNAL   J Biol Chem 275 (48), 37414-37422 (2000)
   PUBMED   10973981
REFERENCE   8  (bases 1 to 4045)
  AUTHORS   Deken,S.L., Beckman,M.L., Boos,L. and Quick,M.W.
  TITLE     Transport rates of GABA transporters: regulation by the N-terminal
            domain and syntaxin 1A
  JOURNAL   Nat Neurosci 3 (10), 998-1003 (2000)
   PUBMED   11017172
REFERENCE   9  (bases 1 to 4045)
  AUTHORS   Clark,J.A.
  TITLE     Analysis of the transmembrane topology and membrane assembly of the
            GAT-1 gamma-aminobutyric acid transporter
  JOURNAL   J Biol Chem 272 (23), 14695-14704 (1997)
   PUBMED   9169433
REFERENCE   10 (bases 1 to 4045)
  AUTHORS   Guastella,J., Nelson,N., Nelson,H., Czyzyk,L., Keynan,S.,
            Miedel,M.C., Davidson,N., Lester,H.A. and Kanner,B.I.
  TITLE     Cloning and expression of a rat brain GABA transporter
  JOURNAL   Science 249 (4974), 1303-1306 (1990)
   PUBMED   1975955
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Nov 27, 2020 this sequence version replaced NM_024371.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M59742.1, SRR26360176.618953.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-54                JAXUCZ010000004.1  149022519-149022572
            55-379              JAXUCZ010000004.1  149023256-149023580
            380-511             JAXUCZ010000004.1  149023901-149024032
            512-612             JAXUCZ010000004.1  149024149-149024249
            613-722             JAXUCZ010000004.1  149025186-149025295
            723-855             JAXUCZ010000004.1  149026021-149026153
            856-990             JAXUCZ010000004.1  149028789-149028923
            991-1094            JAXUCZ010000004.1  149029123-149029226
            1095-1219           JAXUCZ010000004.1  149029665-149029789
            1220-1332           JAXUCZ010000004.1  149030928-149031040
            1333-1464           JAXUCZ010000004.1  149031201-149031332
            1465-1567           JAXUCZ010000004.1  149032523-149032625
            1568-1668           JAXUCZ010000004.1  149033957-149034057
            1669-1836           JAXUCZ010000004.1  149034726-149034893
            1837-4045           JAXUCZ010000004.1  149035630-149037838
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     gene            1..4045
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /note="solute carrier family 6 member 1"
                     /db_xref="GeneID:79212"
                     /db_xref="RGD:620533"
     exon            1..54
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            55..379
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..1941
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /note="GAT-1; solute carrier family 6 (neurotransmitter
                     transporter), member 1; GABA transporter protein; solute
                     carrier family 6 (neurotransmitter transporter, GABA),
                     member 1"
                     /codon_start=1
                     /product="sodium- and chloride-dependent GABA transporter
                     1"
                     /protein_id="NP_077347.1"
                     /db_xref="GeneID:79212"
                     /db_xref="RGD:620533"
                     /translation="MATDNSKVADGQISTEVSEAPVASDKPKTLVVKVQKKAGDLPDR
                     DTWKGRFDFLMSCVGYAIGLGNVWRFPYLCGKNGGGAFLIPYFLTLIFAGVPLFLLEC
                     SLGQYTSIGGLGVWKLAPMFKGVGLAAAVLSFWLNIYYIVIISWAIYYLYNSFTTTLP
                     WKQCDNPWNTDRCFSNYSLVNTTNMTSAVVEFWERNMHQMTDGLDKPGQIRWPLAITL
                     AIAWVLVYFCIWKGVGWTGKVVYFSATYPYIMLIILFFRGVTLPGAKEGILFYITPNF
                     RKLSDSEVWLDAATQIFFSYGLGLGSLIALGSYNSFHNNVYRDSIIVCCINSCTSMFA
                     GFVIFSIVGFMAHVTKRSIADVAASGPGLAFLAYPEAVTQLPISPLWAILFFSMLLML
                     GIDSQFCTVEGFITALVDEYPRLLRNRRELFIAAVCIVSYLIGLSNITQGGIYVFKLF
                     DYYSASGMSLLFLVFFECVSISWFYGVNRFYDNIQEMVGSRPCIWWKLCWSFFTPIIV
                     AGVFLFSAVQMTPLTMGSYVFPKWGQGVGWLMALSSMVLIPGYMAYMFLTLKGSLKQR
                     LQVMIQPSEDIVRPENGPEQPQAGSSASKEAYI"
     exon            380..511
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            512..612
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            613..722
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            723..855
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            856..990
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1094
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1219
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1332
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1464
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1567
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1668
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            1669..1836
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..4045
                     /gene="Slc6a1"
                     /gene_synonym="Gabt1; Gat1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcaggctctg cggagaaagc ctttaggaga agactcatag cagagaacta ttaggccgca
       61 aagctgctgt ccacgtggac tggagctgac atcgcacgcc tgcccgccag gatccctgct
      121 gccaagttcg tgctccgaga catggcgact gacaacagca aggtggctga tgggcagatc
      181 tctaccgagg tcagcgaggc tcctgtggcc agtgacaagc ccaaaaccct tgtagtcaag
      241 gtgcagaaga aggctggaga cctccctgac cgggacacat ggaagggacg cttcgacttc
      301 ctcatgtcct gtgtgggcta cgccatcggc ctgggcaatg tatggaggtt cccttatctc
      361 tgcggaaaga acggtggtgg ggccttccta attccatatt tcctgacgct catctttgcg
      421 ggtgttcctc tcttcctttt ggagtgctcc ctaggccagt acacctccat tgggggcctg
      481 ggcgtatgga agctggctcc catgttcaag ggtgtgggcc tcgcggcagc tgtgctgtcc
      541 ttctggctga acatttatta cattgtcatc atctcctggg ccatctacta cctgtacaac
      601 tccttcacca cgactctgcc gtggaaacag tgtgacaacc cctggaacac tgaccgctgc
      661 ttctccaact atagcctggt caacaccacc aacatgacca gtgccgtggt ggaattctgg
      721 gagcgcaaca tgcaccaaat gacagatggg ctagacaagc caggacagat ccgctggccg
      781 ctagccatca cactggccat tgcctgggtg cttgtgtatt tctgcatctg gaagggtgtt
      841 ggatggactg gaaaggtggt ctacttctca gccacatacc cctacatcat gctcatcatc
      901 ctgttcttcc gtggagtgac acttcctggg gccaaggagg ggatcctctt ctacatcacg
      961 cccaacttcc gaaagctgtc tgattctgag gtgtggcttg acgccgccac ccagatcttc
     1021 ttctcctacg gactgggcct ggggtccctg attgctctgg gaagctacaa ctctttccac
     1081 aacaatgtgt acagggactc catcattgtt tgctgcatca actcctgcac cagcatgttt
     1141 gccggcttcg tcatcttctc catcgtgggc ttcatggctc atgtcaccaa gaggtccata
     1201 gccgatgtgg cggcctcagg ccctggactg gcattcttgg cgtaccccga ggctgtgaca
     1261 cagctaccca tctctcccct ctgggctatc ctcttcttct ccatgctgct gatgctgggc
     1321 attgacagcc agttctgtac cgtggagggc ttcatcactg ccctggtgga tgagtacccc
     1381 aggcttctcc gcaatcgccg tgaactcttc attgctgccg tgtgcatcgt gtcctacctg
     1441 attggcctct ctaacatcac ccagggtggc atttatgtct tcaaactgtt cgattattac
     1501 tctgccagtg gcatgagcct gctgttcctg gttttctttg agtgtgtctc catttcctgg
     1561 ttttatggtg tcaaccggtt ctatgacaac atccaggaga tggttggctc caggccctgc
     1621 atctggtgga agctgtgctg gtcctttttc acacccatca ttgtggcggg cgtgtttctc
     1681 ttcagtgctg tgcagatgac accactcacc atgggaagct acgttttccc caagtggggc
     1741 cagggcgtgg gctggctcat ggctctgtcc tccatggtgc tcatccccgg gtacatggct
     1801 tacatgttcc tcaccctaaa gggctctctg aagcagcgtc tccaggtcat gattcagccc
     1861 agtgaagata tcgtgcgccc tgagaatggt cctgagcagc cgcaggctgg cagctcagcc
     1921 agcaaggagg cctacatcta ggggtacagc cccctcatca cccctacact ggcaccctgg
     1981 actggctgta cccacacccc ttgaagactg aagatactct ctgtctccaa ctacctcaag
     2041 gggcgggtcc agacaccatc accatgcaga gaggggagat gggggacagt ctgaccctag
     2101 gtgggccctg agtgggcagc caccccttgg gggctttcac agaggcccct tagcaggagc
     2161 aggtggctgg cctttgtaac tgccactgta gctccttttt atgctgccag agagggtgat
     2221 ggctgaggcc acacactcct ggcttttagt cttcttcaga ctgttgtcct gtgccccaac
     2281 tgtagactgt tatccagact tttctgtcca ccttggctct gaactggtca tggaccaggg
     2341 ctacagcatt gtgttcatgg ccgctggcag ccggcaggcc ctgcctcttc ctttttaatt
     2401 tctccttccc gaggcagctt cacagaccca tccccagact gttcacagac cagtgcggag
     2461 agggtcctct gtccctgtga ccctgtgatc cgggcagctc agtgtcccct gccccacctc
     2521 cccaggtggc attaacagca aacagcctat ccagaagagc tctgtgtgct gaggagaaaa
     2581 agaaaagcca caagcacaat tgccttttct gatcaccaac ccaggacttt ccccgagtgg
     2641 gagctgtagt ccctctgagc tgcccagttt aacacaaaca atggccaagg aaatcaaggt
     2701 gaaggttcta agtcaggacc aatccagccc accctggttt ccctcttaga ctgtcactgc
     2761 cactctgttc tgctgtcccc ctgcccccct tacctttctg gaaaactttc agatgtacac
     2821 acccactaac ggaggagggt tacttcagag gttccctcca tcctaccctg gcactgagct
     2881 tcccctgctc cagtggggag catcgtatgt cccttctggc cttggattct gtgttggtga
     2941 agccagtgga gacagtttct ggaaagttcc agcagctccg tctttctcct ctgccgagag
     3001 gatggactcc catgtagcaa agcgtatatg tcttgtcccg tagcttctta gcttggtagc
     3061 tcacaaactg tgtttatgac taatccttaa taactatggt gaataactgt gaccgtgggt
     3121 ttcttgaatc tcttgtcatt ctcatccaaa agtgaccagc acaccagttc ttgcaataag
     3181 ctatctccct ccctcagagc atcaagcaca ctgtagagaa tgcaaacacg tacgcacata
     3241 gaaatgcaca cacatgcata cccatcctca catgtggcag ttagcgtccc gtgtgatatt
     3301 gtgtaggcaa tctaccagct cttcccgagg ccacttgtag cagggtcgtg tggctgaggc
     3361 atctgcctgc tcagtggaga ctctgcgacc agcttgcaca ggtgcctgtc gccagagtcc
     3421 caggtgtgga atgcaaggac cctccactgt gtctcgtggc cccgtccctc ccctacctgt
     3481 ctatgaagcc ctttaggatg aaggcaggag gtttccttct tgctgctcga tgttctttga
     3541 tggaaaactg agaacaagtc ttttttgaga taaatgcagt atatttcatg tttgtaagca
     3601 cctctgagat gtttggcaag aaatcccctg atttccaccc aaacttaacc ttatagagca
     3661 caacgttaag ggtcgtacaa ttactgtgag aactgtgaat atgtgtaact tttctttttt
     3721 agtttttgcc cagagggaag aagataattg tattatcata tatgcttttt tttgcaataa
     3781 ggatttattc tcagaacacc aagtaaattt atctctatat aaaaagtata tgtaatatat
     3841 gcctattcag actatataca gagcctgttt taaaaaatta cagtattatt tagtaaaatt
     3901 atctgttcta tggaccaaat gtaaaatatt tatacatgaa gatgtgtttt aaatgtctat
     3961 caaatgatat cacaactaga gcacgggcgt tatgtacgtt tctaagaatt tagaggaaaa
     4021 ataataaagg ttctatgata tatgg
//
