LOCUS       NM_001414948           11986 bp    mRNA    linear   ROD 18-FEB-2024
DEFINITION  Rattus norvegicus solute carrier family 4 member 8 (Slc4a8),
            transcript variant 4, mRNA.
ACCESSION   NM_001414948 XM_017594897
VERSION     NM_001414948.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Wang,W., Tsirulnikov,K., Zhekova,H.R., Kayik,G., Khan,H.M.,
            Azimov,R., Abuladze,N., Kao,L., Newman,D., Noskov,S.Y., Zhou,Z.H.,
            Pushkin,A. and Kurtz,I.
  TITLE     Cryo-EM structure of the sodium-driven chloride/bicarbonate
            exchanger NDCBE
  JOURNAL   Nat Commun 12 (1), 5690 (2021)
   PUBMED   34584093
  REMARK    Erratum:[Nat Commun. 2021 Oct 13;12(1):6083. PMID: 34645810]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   Bernardino,R.L., Martins,A.D., Socorro,S., Alves,M.G. and
            Oliveira,P.F.
  TITLE     Effect of prediabetes on membrane bicarbonate transporters in
            testis and epididymis
  JOURNAL   J Membr Biol 246 (12), 877-883 (2013)
   PUBMED   24105628
REFERENCE   3  (bases 1 to 11986)
  AUTHORS   Coley,A.A., Ruffin,V.A., Moss,F.J., Hopfer,U. and Boron,W.F.
  TITLE     Immunocytochemical identification of electroneutral Na-coupled HCO
            transporters in freshly dissociated mouse medullary raphe neurons
  JOURNAL   Neuroscience 246, 451-467 (2013)
   PUBMED   23500099
REFERENCE   4  (bases 1 to 11986)
  AUTHORS   Siyanov,V. and Baltz,J.M.
  TITLE     NHE1 is the sodium-hydrogen exchanger active in acute intracellular
            pH regulation in preimplantation mouse embryos
  JOURNAL   Biol Reprod 88 (6), 157 (2013)
   PUBMED   23677982
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11986)
  AUTHORS   Burette,A.C., Weinberg,R.J., Sassani,P., Abuladze,N., Kao,L. and
            Kurtz,I.
  TITLE     The sodium-driven chloride/bicarbonate exchanger in presynaptic
            terminals
  JOURNAL   J Comp Neurol 520 (7), 1481-1492 (2012)
   PUBMED   22102085
  REMARK    GeneRIF: These findings suggest that NDCBE can regulate
            glutamatergic transmission throughout the brain
REFERENCE   6  (bases 1 to 11986)
  AUTHORS   Chen,L.M., Kelly,M.L., Parker,M.D., Bouyer,P., Gill,H.S.,
            Felie,J.M., Davis,B.A. and Boron,W.F.
  TITLE     Expression and localization of Na-driven Cl-HCO(3)(-) exchanger
            (SLC4A8) in rodent CNS
  JOURNAL   Neuroscience 153 (1), 162-174 (2008)
   PUBMED   18359573
REFERENCE   7  (bases 1 to 11986)
  AUTHORS   Damkier,H.H., Nielsen,S. and Praetorius,J.
  TITLE     Molecular expression of SLC4-derived Na+-dependent anion
            transporters in selected human tissues
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 293 (5), R2136-R2146 (2007)
   PUBMED   17715183
REFERENCE   8  (bases 1 to 11986)
  AUTHORS   Lee,Y.S., Ouyang,Y.B. and Giffard,R.G.
  TITLE     Regulation of the rat brain Na+ -driven Cl-/HCO3 - exchanger
            involves protein kinase A and a multiprotein signaling complex
  JOURNAL   FEBS Lett 580 (20), 4865-4871 (2006)
   PUBMED   16916513
  REMARK    GeneRIF: Results suggest that Na(+)-driven Cl(-)/HCO(3)(-)
            exchanger (NCBE) activity is inhibited by protein kinase A and
            depends on the integrity of the actin cytoskeleton within a
            multiprotein complex at the plasma membrane.
REFERENCE   9  (bases 1 to 11986)
  AUTHORS   Grichtchenko,I.I., Choi,I., Zhong,X., Bray-Ward,P., Russell,J.M.
            and Boron,W.F.
  TITLE     Cloning, characterization, and chromosomal mapping of a human
            electroneutral Na(+)-driven Cl-HCO3 exchanger
  JOURNAL   J Biol Chem 276 (11), 8358-8363 (2001)
   PUBMED   11133997
REFERENCE   10 (bases 1 to 11986)
  AUTHORS   Schwiening,C.J. and Boron,W.F.
  TITLE     Regulation of intracellular pH in pyramidal neurones from the rat
            hippocampus by Na(+)-dependent Cl(-)-HCO3- exchange
  JOURNAL   J Physiol 475 (1), 59-67 (1994)
   PUBMED   8189393
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000187.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001414948.2.
            
            On Dec 15, 2022 this sequence version replaced XM_017594897.2.
            
            Transcript Variant: This variant (4) encodes the longest isoform
            (4).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132261,
                              SAMD00132262 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-204               JACYVU010000187.1  18598560-18598763
            205-286             JACYVU010000187.1  18615764-18615845
            287-436             JACYVU010000187.1  18627925-18628074
            437-572             JACYVU010000187.1  18628752-18628887
            573-733             JACYVU010000187.1  18629645-18629805
            734-922             JACYVU010000187.1  18634044-18634232
            923-1014            JACYVU010000187.1  18635081-18635172
            1015-1172           JACYVU010000187.1  18635864-18636021
            1173-1260           JACYVU010000187.1  18636632-18636719
            1261-1407           JACYVU010000187.1  18637760-18637906
            1408-1508           JACYVU010000187.1  18638825-18638925
            1509-1683           JACYVU010000187.1  18643489-18643663
            1684-1817           JACYVU010000187.1  18644193-18644326
            1818-2063           JACYVU010000187.1  18645913-18646158
            2064-2169           JACYVU010000187.1  18648143-18648248
            2170-2331           JACYVU010000187.1  18648948-18649109
            2332-2445           JACYVU010000187.1  18651789-18651902
            2446-2607           JACYVU010000187.1  18654771-18654932
            2608-2859           JACYVU010000187.1  18656115-18656366
            2860-2928           JACYVU010000187.1  18658469-18658537
            2929-3102           JACYVU010000187.1  18659380-18659553
            3103-3237           JACYVU010000187.1  18660562-18660696
            3238-3329           JACYVU010000187.1  18662703-18662794
            3330-3425           JACYVU010000187.1  18664067-18664162
            3426-11986          JACYVU010000187.1  18665234-18673794
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q36"
     gene            1..11986
                     /gene="Slc4a8"
                     /note="solute carrier family 4 member 8"
                     /db_xref="GeneID:315311"
                     /db_xref="RGD:735164"
     exon            1..204
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..3438
                     /gene="Slc4a8"
                     /note="isoform 4 is encoded by transcript variant 4;
                     electroneutral sodium bicarbonate exchanger 1; Na-driven
                     Cl-HCO3 exchanger NDCBE1-B; Na-driven Cl-HCO3 exchanger
                     NDCBE1-C; Na-driven Cl-HCO3 exchanger NDCBE1-A; k-NBC3;
                     electroneutral Na+-driven Cl-HCO3 exchanger; solute
                     carrier family 4 (anion exchanger), member 8; solute
                     carrier family 4, sodium bicarbonate cotransporter, member
                     8"
                     /codon_start=1
                     /product="electroneutral sodium bicarbonate exchanger 1
                     isoform 4"
                     /protein_id="NP_001401877.1"
                     /db_xref="GeneID:315311"
                     /db_xref="RGD:735164"
                     /translation="MPAGSNEPDGVLSYQRPDEEAVVDQGGTSTILNIHYEKEELEGH
                     RTLYVGVRMPLGRQSHRHHRTHGQKHRRRGGRGKGASQGEEGLEASAHDTPSQRVQFI
                     LGTEEDEEHVPHELFTELDEICMKEGEEAEWKETARWLKFEEDVEDGGERWSKPYVAT
                     LSLHSLFELRSCLINGSVLLDMRASSIEEISDLILDQQELLRDLSDSVRVKVREALLK
                     KHHHQNEKKRNNLIPIVRSFAEVGKKQSDPHSMDRDGQTVSPQSAPATNLEVKNGVNC
                     EHSPVDLSKADLHFMKKIPAGAEASNVLVGEVDTLDRPIVAFVRLSPAVLLSGLTEVP
                     IPTRFLFILLGPVGKGQQYHEIGRSMATIMTDEIFHDVAYKAKERDDLLAGIDEFLDQ
                     VTVLPPGEWDPSIRIEPPKNVPSQEKRKMPGVPNGNICHVEPEPHGGHSGPELERTGR
                     LFGGLVLDVKRKAPWYWSDYRDALSLQCLASFLFLYCACMSPVITFGGLLGEATEGRI
                     SAIESLFGASMTGIAYSLFAGQPLTILGSTGPVLVFEKILFKFCKDYALSYLSLRACI
                     GLWTAFLCIVLVATDASSLVCYITRFTEEAFASLICIIFIYEAIEKLIHLAETYPIHM
                     HSQLDHLSLYYCRCALPENPNNHTLQYWKEHSIPTADVNWANLTVSECQEMHGEFIGS
                     ACGHHGPYTPDVLFWSCILFFATFIVSSTLKTFKTSRYFPTRVRSTVSDFAVFLTIFT
                     MVILDFLIGVPSPKLQVPSVFKPTRDDRGWFISPIGPNPWWTVIAAIIPALLCTILIF
                     MDQQITAVIINRKEHKLKKGCGYHLDLLVVAIMLGVCSLMGLPWFVAATVLSITHVNS
                     LKLESECSAPGEQPKFLGIREQRVTGLMIFVLMGCSVFMTAVLKFIPMPVLYGVFLYM
                     GVSSLQGIQFFDRLKLFGMPAKHQPDFIYLRHVPLRKVHLFTLVQLTCLVLLWVIKAS
                     PAAIVFPMMVLALVFVRKVMDLCFSKRELSWLDDLMPESKKKKLDDAKKKEEEEEAEK
                     MLDIGGDKFPLESRKLLSSPGKNNSFRCDPSEINISDEMPKTTVWKALSINSGNTKEK
                     SPFN"
     exon            205..286
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            287..436
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            437..572
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            573..733
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            734..922
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1014
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1172
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1260
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1407
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1508
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1683
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1817
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..2063
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            2064..2169
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2331
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2445
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2607
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2859
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            2860..2928
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..3102
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            3103..3237
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            3238..3329
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            3330..3425
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
     exon            3426..11986
                     /gene="Slc4a8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggctgatgg ctatgaaggt ggcgccggtg gatggttgac cggtcgttgg atccagggta
       61 ggggtcgccg cgcgtgcgat cggctcacac aggaccagag ggcgcggact gctgcagctc
      121 aacctggaac ccaccagcga gaccaggctc cgcccggcca tgcccgccgg gagcaacgag
      181 ccggacggcg tcctcagcta tcagagacca gatgaggaag ctgtggtgga tcagggtggg
      241 accagcacga ttctcaacat tcactatgag aaagaagagt tggaaggtca tagaactctg
      301 tatgtggggg ttcggatgcc actggggagg cagagccatc gtcaccaccg aactcatggc
      361 cagaagcacc ggagacgagg agggcggggc aaaggagcca gccagggcga ggagggcttg
      421 gaagcctccg cccacgacac cccgtctcag cgcgtacagt tcattcttgg cactgaggaa
      481 gatgaggagc acgttcctca tgagctgttc acagagctgg acgagatctg tatgaaagag
      541 ggggaagaag ccgaatggaa ggaaacggcc aggtggctga agtttgaaga ggacgtggag
      601 gacgggggag agcgctggag caagccttac gtggccacac tttctctgca cagcctgttt
      661 gagctgagga gctgcctcat caatggctcc gtcctcctgg acatgcgcgc aagcagcata
      721 gaggagatct cagacctgat cctggaccag caggagctgc tccgggacct gagcgacagc
      781 gtgcgggtta aggtgcggga agccttgctg aagaagcatc accatcagaa cgagaagaag
      841 aggaacaacc tcatccccat cgtccgctcc tttgccgagg tcggcaagaa gcagtctgac
      901 ccgcattcca tggacagaga tgggcaaacc gtgtctcctc agtctgctcc agctaccaac
      961 cttgaagtga agaatggtgt gaactgtgaa cacagccctg tggatctaag caaggcggac
     1021 ctccatttca tgaaaaagat tcccgcaggg gcagaggcct ccaatgtcct ggttggcgag
     1081 gtggacactc tggaccggcc catcgtcgct ttcgtgaggc tctccccggc cgtgctcctc
     1141 tctggactga cagaagtgcc catcccaacg aggtttttgt ttatcttact gggtccggta
     1201 ggaaaagggc agcagtacca tgagattggc agatccatgg ccaccatcat gacggatgag
     1261 atttttcacg atgtggcgta taaagcgaaa gaacgggatg atctcctggc tgggatcgat
     1321 gagttcctgg atcaagtgac ggtgctccca ccaggggagt gggacccgtc cattagaatt
     1381 gagccaccca agaatgtccc ttcccaggaa aaaaggaaga tgcctggcgt tccaaacgga
     1441 aacatttgcc acgtagaacc tgagccacat gggggacaca gcgggccgga acttgagcgg
     1501 acggggcggc tgtttggggg cttggtgctg gatgtcaaac ggaaggctcc ctggtactgg
     1561 agtgactacc gggatgctct cagcttacag tgtctggcct ccttcctgtt cctgtactgt
     1621 gcctgcatgt cgcctgttat caccttcggg ggactgctgg gagaagccac ggaaggacgc
     1681 ataagtgcaa tcgaatcgtt gtttggagcc tccatgacgg ggattgccta ctctctgttt
     1741 gctgggcagc ctctcaccat cctgggaagc acgggccctg tgctggtgtt tgaaaagatt
     1801 ttgttcaaat tctgcaagga ctacgccctt tcatacctct ccttgcgtgc ttgtatcggg
     1861 ctgtggaccg ccttcctgtg tattgtcctg gtggcgactg atgccagttc cctcgtctgc
     1921 tacattaccc gcttcaccga ggaagccttt gcctccctga tttgcattat tttcatctat
     1981 gaagccatag aaaagctgat tcacctggcc gagacctacc ccatccacat gcacagccag
     2041 ctggaccacc tgagccttta ttactgcagg tgtgcgctcc ccgagaatcc caacaaccac
     2101 accttacagt actggaagga gcacagcatc ccgactgcag acgtgaactg ggcaaacctg
     2161 actgtcagcg aatgccagga gatgcacggg gagttcatag gatcggcctg tggtcaccat
     2221 gggccctaca cgcctgacgt gctcttctgg tcctgtattc tgttcttcgc taccttcatc
     2281 gtctcaagca ccttgaagac gtttaagacg agccgctact tcccaacgag ggttcgctcc
     2341 acggtgagtg acttcgctgt gttcctcacc atctttacta tggtgatcct agactttctg
     2401 attggcgtcc cctcgcccaa gcttcaggtc cccagcgtgt tcaagccaac gagggatgat
     2461 cgaggatggt tcattagtcc catcggcccc aatccctggt ggaccgtgat agccgcgatt
     2521 atcccagctc tcctgtgcac catcttgata ttcatggacc agcagatcac cgccgtcatc
     2581 attaaccgga aggagcacaa gctcaagaaa ggctgtggct accatctgga cctgctcgtg
     2641 gtggccatca tgctgggggt ctgctccctc atgggcctgc cttggttcgt ggctgcgacc
     2701 gtgctgtcca ttactcatgt gaatagcctc aagctggaat ctgaatgctc tgctccgggg
     2761 gagcagccta agtttctggg catccgagaa cagagagtca cgggcctcat gatctttgtg
     2821 ctgatgggct gctccgtctt catgacagcg gtcttaaagt ttatcccaat gccagtgctc
     2881 tacggagttt tcctctacat gggagtctct tctctacaag gaatccagtt ctttgaccgt
     2941 ctgaagcttt ttgggatgcc tgcaaaacac cagccagact tcatctacct gcggcacgtg
     3001 ccgctgcgca aagtgcacct cttcacgctg gtccagctca cctgcctcgt cctgctctgg
     3061 gtcattaagg cgtcaccggc tgccattgtt ttcccaatga tggtcctggc cttggtcttc
     3121 gtgaggaagg tcatggacct ctgcttctcc aagcgtgagc tgagctggct ggacgacctc
     3181 atgcctgaga gcaaaaagaa gaagctggat gatgcgaaga agaaggagga ggaggaggag
     3241 gctgagaaga tgttggatat tgggggtgat aagttccccc tggaaagcag gaagttgcta
     3301 agtagtcctg gcaagaacaa cagcttcaga tgtgaccctt ctgagattaa tatatcagat
     3361 gaaatgccta aaaccacagt ctggaaggct ctcagcataa attctggaaa cacgaaagaa
     3421 aaaagcccct tcaattgaga gtctccgctg ggtggaagac ttgggccatg aggtgcctcg
     3481 gagaagttct gggctttgag aaaatggcga gtctctccgg gaagaagccg ggctgagtct
     3541 ggccctgttc ttggtcatct cgaagccaca tggaagcatt cagtcactcc cggtgtgcgt
     3601 ttggagggta tccagctacc ccacatacca gcagccaggc accagccagg ccactgtggg
     3661 gccacctcct gctgcctcct ttcctcctcc ttctttctct tcagagctgc caccattaaa
     3721 gagccagctt ccactttcag acattttctc tgatgctctg ctggcctggt aataagccac
     3781 atgagtccat tctgtcatgg aggattcctt cgtgcggtcc ctcttcctca acgacagccc
     3841 ggggtagtgg cagggaggca ggacaagggc aggcttacga gcagcctgtg cctctgtctc
     3901 ccttctgctt gggaaaggga tgttggacag acagacactg cagcaggtct gctacctgag
     3961 ctctaccatc caggcatcaa cagcaactac ttgcctctgg cctggggtca cccgcatcac
     4021 ctccagaagc agtcagtgtg atgtcatgag accatgggtc acccgcatca cctccagaag
     4081 cagtcagtgt gatgtcatga gaccatgggt cacccgcatc acctccagaa gcagtcagtg
     4141 tgatgtcatg agaccatggg tcacccgcat cacctccaga agcagtcagt gtgatgtcat
     4201 gagaccttgg gtcacccgca tcacctccag aagcagtcag tgtgatgtca tgagacctgg
     4261 ggtcacccac atcgcctcca gaagcaatcc atgtaatgtc atgagaccct ggaagatttg
     4321 ggaacagcat gagagcagga gttttgctga tttcaggaag ggcccagggg aagccagcag
     4381 cggtaggtac tgagtgagcg tctgtcctcg ggtttccttg gaaacagaga gggaggagcc
     4441 taacttgcta tggaaggact tggacttcga ggctgaagtt ttgttctagg tctgttttgt
     4501 ctagggaaga ctcgctgaca actgttggtt ggtaggaagg tgggtctggg ttgttacggc
     4561 ttttctgttt gacacgctgc tgccatctct cgaggagacg ttgttattcc accctggagc
     4621 gacccaagtg ctcatggatg tcccttagct gccttagtgg agagcaccat catcatcagc
     4681 ccagaggacc aaaaccacca tcagccaggt ggcttcctca ccgtggggtt cttttggttg
     4741 acagaagttc tggctcagat gtaaaagcca cacgaagaag tgaactataa gcggtgaagt
     4801 ccatttggat acttaggtta acagtgtgtg tacgattttt ctaagttcca gaactgtcat
     4861 cagttttgtt ggcccaagca ctgtagacat ccccttcctt tgtgcacaga acgtgaccag
     4921 tgagctggct gtgctgttgt ccaactggga cagtctctgt acagaggcca cacacagtgt
     4981 gctgtcctcc tttcctgggt gaagaagcac cacatcattc agtcgcagtt tgctcccttc
     5041 agactccgag gcatccagtc ctcctctgaa ggcctttggt gtcaggggcc accccacagt
     5101 gccagttcct ccctccctgt caggaccact gccatcttga tgcaagacgt ttgacaattg
     5161 gtacaggaca gacaggtgtc ttctgtaaga tagtgggagg cagtgcttgc tgttcaggac
     5221 ctgtgggatg gatgtgaccc cactttcaaa tcccaaaatg gcctctggaa tgtgggcaag
     5281 gttcctggat tgtctgctct ttctttaccc aggagagacc tgtgttccat ctcagcagcc
     5341 gctgtggcct gctctcagat agtacgtact ggaacaggca ggtttcttag acatcagtgg
     5401 gcattaaagt ctcggtgagc caaattcccc aagcatatcg cttgaggaca ggtctcataa
     5461 gcccttgccc accttgggtt cagagccagc ctgtcaacag gaagggttga ttccttccct
     5521 gccctccaga cactttctgg agctcagttg tccctcgctg ctcacattct ctggaaacac
     5581 cccattttct tccccgactt gtctctcctc tatcctcctg ccggcttctg tgtagggaaa
     5641 tggatgactg cttcccctgc tctagcctca gtgagcctga gagacttgta acagcgagga
     5701 gcctgttttg cgagggaggc tgaaaaactg tctcttcccg ctcaaggatg cttgtgttgc
     5761 ctcctgtgag ttactccctg ggctggagag tgaagggtgc tttccctgac tccaaataat
     5821 gcagttgtgg gacctcaggc ccacatttgc aggaaccacc gaggtggcca tctcttctac
     5881 gacaagggtc actttgagac atgtcttgaa tgttgccagc taacatcctg acacgctggt
     5941 gatgtcagct ggtgatgcaa atggagttgg gggaggctgg gattcaagaa catgtaagtg
     6001 aagccttggc tctgagggac aagatggagg gttcctaagt ggccggttgc ttcccgatga
     6061 ccatcagaga agggtcgctc tagagaaagg tgaagcgttg gagagacata atgtgcagtc
     6121 gccaaatgtg tttggttgtg taacacacgg aacttgggaa gctttgtcag gaggctcggc
     6181 ctttccaatc cagctccgtg ctgggtctcc ttgatcatga aaacctgcct tcctgatgga
     6241 aaatgaacca tctctctcca aaagacagtg tgagcagttg cagtttctca tcggtgatgg
     6301 agcagacttt taagtcactc ataaaggttg ggaaaatgtg gtattcgcca ggttccccac
     6361 ctgcctagac tctgcagagt ctgtggttta tggtcgctca acaaaggacg gttcctgact
     6421 ccgctttatc ctcccccccc cccccccccc gcttagctct ccgacacccc tcttagcggg
     6481 gaggcagggg ttctcctgtg ttgtggctgc agtgtttgtc ctgcacctcc acttgtctgg
     6541 gcgtgcttta gttcgtaatg agtggcagtg acaccgtctc ctgtgtgtct cctgccactt
     6601 tgccattgtc cctgaaggtc ttttgcaaga gcagcagcac ctcctcctag aactttgaat
     6661 tttaatgtaa ctaagttgta tgtgtgtgtg tgttagtctt tgttccacgt gtatccttca
     6721 agaaatcagg aggaatcaga tcctccggaa actcgagtta cacatagttg tgagcttctg
     6781 tgtgggtgct gggaatttag cttaacttgg gtgtagctgg tctttgttac gttgtgagtt
     6841 attttctcac cctttgctcc ctcccccagt ttcactccct gacactggat agggcagaag
     6901 cgaggagaga tccctaaatc taaattcctt ttgtttcttc ttagagcttg actactaaga
     6961 agccacaacc aacacccccc aaaccctcca ccctgccccc cagtgactga ctgacaaccc
     7021 cccaccctac cttttggggg cctggcatta atagaccttc tgaaaagttc ctggaattcc
     7081 caacatgaca caatctcaga aactgtctgc agctggcaaa accacgcttc tgctaaagca
     7141 cgaggcagat catgtcccct gctgcggaca gtccgaagca gccccacacc cccacacccc
     7201 cacacttggg gattaaaata acattcttaa tatttctggt tttttttaag aagccaaaat
     7261 tccagagtcc ctactgtctc cccatttctg ctttaagcca ttaatggtgc tgtgtaccag
     7321 gaaattataa gcatgttttc aagacctaag tgtccaggac agtggccaag gttttcagga
     7381 gccctccctg ttcagttcta atctttaacc actgcttttc agtacctgtg gaaatataaa
     7441 caattccccg tccccgacgc ggaactcttg taggcttctg tctagtatta acacgtcttt
     7501 gtactatatt gtagaaaaaa caaatctgtt ttcctctcag cccctgctcc gggaatggcc
     7561 actcggaggc tgctattaaa tgccttggcc acagcgttgg ctcatccctg actagctcat
     7621 aacgtattga acccactcaa actattctat gtcatccacg tggtggttag cctgcctcag
     7681 tcccggtcgc ctcttccttc tcgcctccac agctctcctg catctcctta tatcctctca
     7741 ctgtctccca gaatcctctc ttccggccag tgtcccacct cctatttctt gcctcagctc
     7801 attggccact ggcattttta ttgacaagag atgcttcttc agcatatagg cagatgtaat
     7861 tccacagtgt tttctatgac ccagtgtctc tcagctgctg ttcctttctc actaatattc
     7921 agaaaattta gagttagaga gctactgatt ctgttttctc tttgttttat aagcgtttaa
     7981 ggtacagtga gatcgatcgt tctacaactg tctgtcctgt aggattgctt gagtattaac
     8041 ctgagcatcc cactattata aaagagccct tccccataga cctggggcat gtcagccaca
     8101 tgaggtctta actttcctgc ttcacctcct ggtcccacac atttcaccca tcacacactt
     8161 tgtcttatct gagataactt tccagttccc agcaactgtg tataaacctt ttgaagtggt
     8221 cattctgaat cccaagattt ttgtgaaatg atagttatct cagctcctgt atccaccaag
     8281 gcgtctgctt cgataccttt tatgtgaaat cttagtgttg gcctctgatc gtcaactgtt
     8341 tgccagaaca caggtttcca aatctcttct gttcgttcta ccggggtgtg gtgtagggag
     8401 agagtagcag ctgagcatcc tatcacctgc atcaagtcag tctctccccc cccccccctc
     8461 tctctctccc ctcccccccc acccctccat aggccataat tttaatttcc cctttgaaat
     8521 cttcatttat aatccgggga tgtgcagtga gtccttagga agtcaattcg cttcttcccc
     8581 agaccgtccc tactgtccgc gcaggcaaag ggccattgtt acattgttac attgaaggtt
     8641 gggggataaa gaaagatgtt tctctgtggc caagtgtaga gcagcggtgc ctgcagtagc
     8701 ttcattaaat ccctggtgct gagtggaggg ttttggtctg tatgttcctc cttgactgaa
     8761 acagctcaca ggctttgggg ggaggggggt aagcgaggcc cccaaggccg tttccttgaa
     8821 tatctctttt ggatctgggt tcctgaattc agtggctgcc tttgccagag tgcacacatg
     8881 tcgcaagaag cctaggcatc ctttctgttt cagatttagg aaaaccattg cctctagaaa
     8941 tggcgtattc acttttctgc tgcaaagtat tgtctttctc gaaattgctc gcacccaggc
     9001 attttgagat ctgaaacttt tggtttggtc acagtctctt tgcaaacgac cgtatttcct
     9061 gaaaatgaag caccaggcat tttgagatca gaggctttta gctatagctt gacttgttat
     9121 atcagcatgg tatgcattag aaccagtgtc ggtcataccc ctcattcatt ctgccataga
     9181 cactgctcga gcttttaatg cctaataact tcttcacatt tggtttttgc cttttcaacc
     9241 accaaggtct ctgtcaacac ctgcctcata tcagggtctg atacagcttc gttcacagtg
     9301 gagccaatct ttgtaatcag tgaaggcttc tccagagcct tgtataatct ttgtgaatga
     9361 ggtggtcttt ttcctgggcc cctcaattgt cacaagcttt gaaaaccact aaggaacatt
     9421 gttccatagt gacactatga aattgagtct gtttcttgtc tgtcactact gcccttcagc
     9481 cagcaactgt agggctgagg tgggttggtt cagatagcga ccgttggagc tgctgcctgt
     9541 ggctccaggg actacagaaa agaatgacag ggaaatcata cactgagtga ctgaagcaag
     9601 gcaaactcca acacctttgt gtttgccttt agctctttta taccccaaat tctctaaaat
     9661 cattcaccac aaaacatatt caaaacacag attaaaatca ctacacagac ggaaattaca
     9721 gcgggatttt tgattacata ttcttcttta ggaacgtcca cctaactcta cattcctcat
     9781 ctgcctttct ctccacaagt tcatggtcac ccaaagcaat aatcctcttg tcatagatta
     9841 acaagcttaa acaggccaag ttgagagtag taggattctc cccaagattc tccccaagct
     9901 tagctggtaa cattgtattt attaaggaac aggtcatctc cccatactta gctggtaaca
     9961 ttgtatttac taaggaacag gtcatcaaca catgcttagc tggtgacatt gtatttacta
    10021 aggaacagtc ctcgtcgtct gtctcgcatc ttatttttga agccttggtc agctagactg
    10081 gctagtcatc tgctctcggt tcttactgca cagtgaagtc accagccctt tgttcatgac
    10141 cttccctctc ctggtgcaca cagaagcctg tggcccaacc cgttggtcac aagatcaggg
    10201 aactcaggcc caacctggaa tgaatgtctc gtttgattgc taccttgtcc caaggccatt
    10261 ttctcctaca gtcacctgct tgtagcaccg agggctcact ggccacctca cctgtgcagc
    10321 ctatgaggaa gggccacact ctatccctga ttcgcacctt aaactctcta tcacctactc
    10381 ttaacttccc aaaactacct taaatcagaa attccataaa atcatcaatt catctatgaa
    10441 cagcactatc tcaagaatgt tcaccatctt gttgacgagc aggaaacttg cccaacagag
    10501 tgggctgtca cccaggaagt agtcacacca ggctatagag gcagtgtgtg tgtgtgtgtg
    10561 tgtgtgtggg tgtctccccg agcccagtca catcacgtca agtatgaggg atatagcaat
    10621 gagaaacccg agcccacaca tcagtaacaa gaagcaacaa caaagcccct ggggctggtc
    10681 atctccaggg cacacccatc gcagccaagc aaagcggtgg gccatctcca ggaagctcac
    10741 ttgcccactg cagctcttcc cgagtggggc tcaccatccc tcggaggaaa gaatcctaat
    10801 ccggagctcc tcgcccgctg ctaaggtgag agctgccagg ccagccgcct ctgatgtcct
    10861 cagcaaaggg actggatggc tgtgttatga caggacaagc agagggacag cacattctta
    10921 agctcccatg gtcacttgtg ttgatctccg ttcacggtaa acaggaaaac acacccaaag
    10981 accaaacagg caaactttct actgcagcca taaacggtgc caggcgtctg cctccagcac
    11041 cttctgtgag acagggtgtc actgtgtaga gcaagagagc cctgacctcg cgccaacttc
    11101 ctttcctccc agctgcagag ccaagcatga gccaaccatg cctggcttcg cccttggtct
    11161 ctcttaggct gacccagttc ttcttctata cctttttctt tgtggccgga aaggattggt
    11221 ttggccgggg tgggggtggg ggtggggggt gggggtgggg ggtgtccctc tgaagagaaa
    11281 aggaacagca tcctccaccc cagatctaac ccactctgag gattggtgaa tacagagcgt
    11341 ttgcccttaa gtggtgtttc ctgagcaaca gggaggccca ccctcctcac acccgcagcc
    11401 cttttgtaag cccagcttgg gcattgctta gagggtttat gggtgactct ggcatgcacg
    11461 gttttacttg tgcaacagtg caagcaccaa catgagtgtg cggtggcaca ctcctcttgt
    11521 gaaaagccaa aattcttcct gttgctggcc acttcttccg gaggctgggc ctccctctca
    11581 tctctgtctg ttgattagaa gtggctgatg gtgtccaatg agagcagagc aaccttccca
    11641 tggaaggggc accttgcggt gctggtgcga atccctcatt ttctatgttc ttctctctag
    11701 taggtctcaa gcagagacag atatttttgt tttagagatt ccaaagtcta tatttttagt
    11761 gtaagaaacg cacccttctc cacacttcat gacgtaaata gaacggggaa taaagtgtcc
    11821 cgctgtgatt accccatagc agtttatgta tggtagaaac aaggcccctt ctccagtgtg
    11881 tctgtggccc agggcaggta actgtgacag tgtatgtctg agcctctgcc tgccctcact
    11941 gtgtgtgctc agctctttgg gtgacaaata aagcccgtat tataaa
//
