LOCUS       NM_058211               7803 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus solute carrier family 4 member 7 (Slc4a7),
            transcript variant 1, mRNA.
ACCESSION   NM_058211
VERSION     NM_058211.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Severin,M., Pedersen,E.L., Borre,M.T., Axholm,I.,
            Christiansen,F.B., Ponniah,M., Czaplinska,D., Larsen,T., Pardo,L.A.
            and Pedersen,S.F.
  TITLE     Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the
            plasma membrane, centrosomes, spindle and primary cilia
  JOURNAL   J Cell Sci 136 (7) (2023)
   PUBMED   37039101
  REMARK    GeneRIF: Dynamic localization of the Na+-HCO3- co-transporter NBCn1
            to the plasma membrane, centrosomes, spindle and primary cilia.
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Olesen,C.W., Vogensen,J., Axholm,I., Severin,M., Schnipper,J.,
            Pedersen,I.S., von Stemann,J.H., Schroder,J.M., Christensen,D.P.
            and Pedersen,S.F.
  TITLE     Trafficking, localization and degradation of the Na+,HCO3-
            co-transporter NBCn1 in kidney and breast epithelial cells
  JOURNAL   Sci Rep 8 (1), 7435 (2018)
   PUBMED   29743600
  REMARK    GeneRIF: Trafficking, localization and degradation of the
            Na(+),HCO3(-) co-transporter NBCn1 in kidney and breast epithelial
            cells
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7803)
  AUTHORS   Ng,F.L., Boedtkjer,E., Witkowska,K., Ren,M., Zhang,R., Tucker,A.,
            Aalkjaer,C., Caulfield,M.J. and Ye,S.
  TITLE     Increased NBCn1 expression, Na+/HCO3- co-transport and
            intracellular pH in human vascular smooth muscle cells with a risk
            allele for hypertension
  JOURNAL   Hum Mol Genet 26 (5), 989-1002 (2017)
   PUBMED   28087731
  REMARK    GeneRIF: The finding of a genotypic influence on SLC4A7 expression
            and pHi regulation in vascular smooth muscle cells provides an
            insight into the molecular mechanism underlying the association of
            variation at the SLC4A7 locus with blood pressure
REFERENCE   4  (bases 1 to 7803)
  AUTHORS   Yao,H., Azad,P., Zhao,H.W., Wang,J., Poulsen,O., Freitas,B.C.,
            Muotri,A.R. and Haddad,G.G.
  TITLE     The Na+/HCO3- co-transporter is protective during ischemia in
            astrocytes
  JOURNAL   Neuroscience 339, 329-337 (2016)
   PUBMED   27717805
REFERENCE   5  (bases 1 to 7803)
  AUTHORS   Coley,A.A., Ruffin,V.A., Moss,F.J., Hopfer,U. and Boron,W.F.
  TITLE     Immunocytochemical identification of electroneutral Na-coupled HCO
            transporters in freshly dissociated mouse medullary raphe neurons
  JOURNAL   Neuroscience 246, 451-467 (2013)
   PUBMED   23500099
REFERENCE   6  (bases 1 to 7803)
  AUTHORS   Kwon,T.H., Fulton,C., Wang,W., Kurtz,I., Frokiaer,J., Aalkjaer,C.
            and Nielsen,S.
  TITLE     Chronic metabolic acidosis upregulates rat kidney Na-HCO
            cotransporters NBCn1 and NBC3 but not NBC1
  JOURNAL   Am J Physiol Renal Physiol 282 (2), F341-F351 (2002)
   PUBMED   11788449
REFERENCE   7  (bases 1 to 7803)
  AUTHORS   Vorum,H., Kwon,T.H., Fulton,C., Simonsen,B., Choi,I., Boron,W.,
            Maunsbach,A.B., Nielsen,S. and Aalkjaer,C.
  TITLE     Immunolocalization of electroneutral Na-HCO(3)(-) cotransporter in
            rat kidney
  JOURNAL   Am J Physiol Renal Physiol 279 (5), F901-F909 (2000)
   PUBMED   11053051
REFERENCE   8  (bases 1 to 7803)
  AUTHORS   Pushkin,A., Clark,I., Kwon,T.H., Nielsen,S. and Kurtz,I.
  TITLE     Immunolocalization of NBC3 and NHE3 in the rat epididymis:
            colocalization of NBC3 and the vacuolar H+-ATPase
  JOURNAL   J Androl 21 (5), 708-720 (2000)
   PUBMED   10975418
REFERENCE   9  (bases 1 to 7803)
  AUTHORS   Choi,I., Aalkjaer,C., Boulpaep,E.L. and Boron,W.F.
  TITLE     An electroneutral sodium/bicarbonate cotransporter NBCn1 and
            associated sodium channel
  JOURNAL   Nature 405 (6786), 571-575 (2000)
   PUBMED   10850716
REFERENCE   10 (bases 1 to 7803)
  AUTHORS   Kwon,T.H., Pushkin,A., Abuladze,N., Nielsen,S. and Kurtz,I.
  TITLE     Immunoelectron microscopic localization of NBC3 sodium-bicarbonate
            cotransporter in rat kidney
  JOURNAL   Am J Physiol Renal Physiol 278 (2), F327-F336 (2000)
   PUBMED   10662737
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On Nov 8, 2022 this sequence version replaced NM_058211.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643299.56218.1, AF080106.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-212               JAXUCZ010000015.1  13095081-13095292   c
            213-294             JAXUCZ010000015.1  13072646-13072727   c
            295-441             JAXUCZ010000015.1  13070595-13070741   c
            442-580             JAXUCZ010000015.1  13066559-13066697   c
            581-741             JAXUCZ010000015.1  13065728-13065888   c
            742-930             JAXUCZ010000015.1  13064159-13064347   c
            931-1299            JAXUCZ010000015.1  13061222-13061590   c
            1300-1415           JAXUCZ010000015.1  13057802-13057917   c
            1416-1573           JAXUCZ010000015.1  13055599-13055756   c
            1574-1661           JAXUCZ010000015.1  13054131-13054218   c
            1662-1808           JAXUCZ010000015.1  13052169-13052315   c
            1809-1915           JAXUCZ010000015.1  13049416-13049522   c
            1916-2090           JAXUCZ010000015.1  13047231-13047405   c
            2091-2224           JAXUCZ010000015.1  13044101-13044234   c
            2225-2470           JAXUCZ010000015.1  13042783-13043028   c
            2471-2576           JAXUCZ010000015.1  13038423-13038528   c
            2577-2738           JAXUCZ010000015.1  13037647-13037808   c
            2739-2852           JAXUCZ010000015.1  13037011-13037124   c
            2853-3014           JAXUCZ010000015.1  13035269-13035430   c
            3015-3266           JAXUCZ010000015.1  13034928-13035179   c
            3267-3335           JAXUCZ010000015.1  13031770-13031838   c
            3336-3509           JAXUCZ010000015.1  13029934-13030107   c
            3510-3641           JAXUCZ010000015.1  13027410-13027541   c
            3642-3739           JAXUCZ010000015.1  13023762-13023859   c
            3740-3847           JAXUCZ010000015.1  13021694-13021801   c
            3848-7803           JAXUCZ010000015.1  13015854-13019809   c
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p16"
     gene            1..7803
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /note="solute carrier family 4 member 7"
                     /db_xref="GeneID:117955"
                     /db_xref="RGD:621208"
     exon            1..212
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..3929
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sodium bicarbonate cotransporter 3; NBC-like protein;
                     electroneutral sodium bicarbonate cotransporter 1; solute
                     carrier family 4, sodium bicarbonate cotransporter, member
                     7; solute carrier family 4 sodium bicarbonate
                     cotransporter member 7 variant NBCn1-J; solute carrier
                     family 4 sodium bicarbonate cotransporter member 7 variant
                     NBCn1-I; solute carrier family 4 sodium bicarbonate
                     cotransporter member 7 variant NBCn1-G"
                     /codon_start=1
                     /product="sodium bicarbonate cotransporter 3 isoform 1"
                     /protein_id="NP_478118.1"
                     /db_xref="GeneID:117955"
                     /db_xref="RGD:621208"
                     /translation="MEADGAGEQMRPLLTRGPDEEAVVDLGKTSSTVNTKFEKEELES
                     HRAVYVGVHVPFSKESRRRHRHRGHKHHHRRRKDKDSDKEDGRESPSYDTPSQRVQFI
                     LGTEDDDEEHIPHDLFTEMDELCYRDGEEYEWKETARWLKFEEDVEDGGDRWSKPYVA
                     TLSLHSLFELRSCILNGTVMLDMRASTLDEIADMVLDNMIASGQLDDSIRENVREALL
                     KRHHHQNEKRFTSRIPLVRSFADIGKKHSDPHLLERNGEGLSASRHSLRTGLSASNLS
                     LRGESPLSLLLSHLLPSSRAGTPAGSRCTTPVPTPQNSPPSSPSLSRLTSRSSQQTQP
                     QAPEVLVSPDRDDIPRVVIHPPEEDIEALKGQEQKNEENTDFTPGILASPQSAPGNLD
                     SSKSGEVKGNGSGGSRENSTVDFSKVDMNFMRKIPTGAEASNVLVGEVDFLERPIIAF
                     VRLAPAVLLSGLTEVPVPTRFLFLLLGPAGKAPQYHEIGRSIATLMTDEIFHDVAYKA
                     KDRNDLLSGIDEFLDQVTVLPPGEWDPSIRIEPPKSVPSQEKRKIPAFPNGSAPVSAD
                     PPKEADHHAGPELQRTGRLFGGLILDIKRKAPFFLSDFKDALSLQCLASILFLYCACM
                     SPVITFGGLLGEATEGRISAIESLFGASLTGIAYSLFAGQPLTILGSTGPVLVFEKIL
                     FKFCRDYHLSYLSLRTSIGLWTSFLCIVLVATDASSLVCYITRFTEEAFAALICIIFI
                     YEALEKLFHLGEIYAFNMHNNLDALTSYTCVCAEPSNPSNETVELWERKNVTAASISW
                     ANLTVSECKTFHGMFVGSACGPHGPYVPDVLFWCVVLFFTTFFLSSFLKQFKTKRYFP
                     TKVRSTISDFAVFLTIVIMVAIDYLVGIPSPKLHVPEKFEPTDPSRGWIISPLGDNPW
                     WTLLIAAVPALLCTILIFMDQQITAVIINRKEHKLKKGAGYHLDLLMVAVMLGVCSIM
                     GLPWFVAATVLSISHVNSLKVESECSAPGEQPKFLGIREQRVTGLMIFILMGLSVFMT
                     SVLKFIPMPVLYGVFLYMGVSSLKGIQFFDRIKLFGMPAKHQPDLIYLRYVPLWKVHV
                     FTVVQLTCLVLLWVIKASAAAVVFPMMVLALVFVRKLMDLCFTKRELSWLDDLMPESK
                     KKKEDDKKKKEKEEAERMLQGDGDTVHLPFERGSLLQIPVKTLKYSIDPSVVNISDEM
                     AKTAQWKALSMNTENAKVTRPNMSPEKPVSVTINFEDEPSKKYMDAETSL"
     exon            213..294
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            295..441
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            442..580
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            581..741
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            742..930
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1299
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1415
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1573
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1661
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1808
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..1915
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2090
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2091..2224
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2225..2470
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2471..2576
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2577..2738
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2739..2852
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            2853..3014
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3015..3266
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..3335
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3336..3509
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3510..3641
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3642..3739
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3740..3847
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
     exon            3848..7803
                     /gene="Slc4a7"
                     /gene_synonym="NBC3; NBCn1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtcctcgcg gcacgccgtt gcttctcacc ctgagaacgc ccctcgcccg cagtcggcgc
       61 ctcactccgc ggccagctct ccgcggccag cgctccgcac agggtcgccc tccgcgtgcc
      121 cgtctctgcc gcggtcgcgg cctctcagag ctcccaggcg ggccatggag gcagacgggg
      181 ccggcgagca gatgagaccg ctactcacgc ggggtcccga tgaagaagct gttgtggatc
      241 ttggcaaaac tagctcaacc gtgaacacca agtttgaaaa agaagaatta gagagtcatc
      301 gagctgtata tgttggtgtt catgtaccgt ttagtaaaga gagtcgtcgg cgtcacaggc
      361 atcgagggca caaacatcac caccggagaa gaaaagacaa agactcagat aaggaagatg
      421 gacgggagtc tccttcttat gacacgccat cgcagagggt gcagttcatc cttgggactg
      481 aagacgatga tgaggagcac attccccacg acctcttcac ggagatggac gagctctgct
      541 accgagacgg ggaagagtac gagtggaaag agacagccag gtggctgaag ttcgaagagg
      601 atgttgagga tggtggtgac cgatggagta aaccgtatgt ggccactctg tctttgcaca
      661 gtctctttga gctgaggagt tgtatcctaa atggaacagt catgctggat atgagagcaa
      721 gcactctgga tgaaatagca gatatggtgt tagacaacat gatagcgtct ggccagctag
      781 atgactccat aagggagaat gttcgagaag ctcttctgaa gagacatcat catcaaaatg
      841 agaaaaggtt tacaagtcgg attcccctcg ttcgatcctt tgcagatata ggcaagaaac
      901 attctgaccc tcacttgctt gaaaggaatg gggaaggcct gtcagcctcc cgccattctt
      961 tgcgaacagg tctgtctgcc tcaaaccttt ccttgagagg agaatcgcct ttatcccttc
     1021 ttctcagtca tcttcttcct tcttcaagag ctggcacccc tgcaggctca aggtgtacca
     1081 ccccagtacc caccccccag aacagtcctc cttccagccc cagcttaagt cgcctgacct
     1141 ccagaagttc ccaacagact cagcctcagg ccccagaagt actggtgtca cctgacaggg
     1201 atgatattcc cagagtagta attcatccgc ctgaggaaga catagaagca ctgaaaggcc
     1261 aagagcagaa gaatgaggaa aatactgact tcactccagg gattttggct tctccacagt
     1321 ctgctcctgg aaacctggac agtagtaaaa gtggtgaagt gaaaggtaac ggaagtggag
     1381 gaagcagaga aaatagtact gttgacttta gcaaggttga catgaatttc atgaggaaaa
     1441 ttcctacagg agctgaggca tccaatgttt tggtaggaga ggtggatttc ttggagagac
     1501 ctatcattgc atttgtgaga ctggctcctg cagttctcct ctcagggttg actgaggtcc
     1561 ctgtgcccac taggtttttg tttctgttac tgggcccagc aggaaaggct ccacagtacc
     1621 atgaaattgg cagatccata gcaactctaa tgacagatga gatttttcat gatgtagctt
     1681 ataaagcaaa agatcgaaat gacctcttat ctggaattga tgaattttta gatcaagtaa
     1741 ctgttcttcc tccaggagag tgggatcctt ccatacgcat agagccacca aaaagtgttc
     1801 cttctcagga gaaaaggaag attcctgcgt ttcccaatgg atctgctcca gtgtctgctg
     1861 accctcctaa ggaggctgat caccacgctg ggcctgagct gcagaggact ggacggcttt
     1921 ttggtggttt gatacttgac atcaaaagga aagcaccttt tttcttgagt gacttcaagg
     1981 atgcattaag tctgcagtgc ctggcctcga ttcttttcct atactgtgcc tgtatgtctc
     2041 ctgtaatcac ttttggaggg ctgcttggag aagctacaga aggcagaata agtgcaatag
     2101 agtctctttt tggagcatca ttaactggga ttgcctattc attgtttgct gggcaacctc
     2161 taacaatact ggggagcacg ggtccagtcc tagtgtttga aaaaatttta tttaaattct
     2221 gtagagatta tcacctatcc tatctatcat taagaaccag tattggtctg tggacttctt
     2281 tcttgtgcat tgtgttggtc gcaacagatg ccagcagcct tgtttgttac attactcggt
     2341 tcacagaaga agctttcgcc gcactcattt gtatcatctt catctatgaa gccttggaga
     2401 aactctttca cttaggagaa atatatgcat ttaacatgca caacaacttg gatgcattga
     2461 ccagttacac atgtgtatgt gctgagccat ctaatcctag caacgaaact gtagagctgt
     2521 gggagaggaa gaacgtgaca gcagccagta tttcctgggc aaaccttacc gtgtctgagt
     2581 gtaagacctt ccacggtatg tttgtgggat cagcttgtgg gcctcacggg ccttatgttc
     2641 ccgatgtgct cttctggtgt gtcgtcttgt ttttcacaac gttctttctg tcttcattcc
     2701 tcaagcagtt taagaccaag agatattttc ctactaaggt gcgatcaaca atcagtgact
     2761 ttgctgtatt tctcacaata gtaataatgg ttgcaattga ctaccttgta ggaattccat
     2821 ctcctaaact tcatgttcct gaaaagtttg agcctactga tccaagcagg ggctggatca
     2881 taagcccttt gggagataac ccttggtgga ccttactaat tgcagctgtt ccagctctcc
     2941 tttgtaccat tctcatcttc atggaccagc agatcacagc tgtgatcatc aacaggaaag
     3001 aacacaaact gaagaaagga gctggctatc acctggactt gctcatggtt gctgtcatgt
     3061 tgggagtctg ctccatcatg ggcctgccgt ggtttgtggc tgcaacagtg ttgtctataa
     3121 gtcatgtcaa cagcttaaaa gtagagtctg aatgttctgc tccaggggaa cagcccaagt
     3181 tcctcggaat tcgtgaacag cgggttacag ggctgatgat ttttatcctg atgggcctct
     3241 ctgtgttcat gacttcagta ttaaagttta ttccgatgcc agttctatat ggtgttttcc
     3301 tttatatggg agtttcttct ctgaaaggaa ttcagttttt tgaccgtatc aaattatttg
     3361 gaatgcctgc caagcaccag ccggacctga tctacctccg ctatgtccct ctctggaagg
     3421 tgcacgtgtt cacggtcgtc cagctgacct gcctggttct gctctgggtg atcaaagcct
     3481 ctgctgctgc agtagttttt cccatgatgg ttcttgcatt agtctttgtg cgcaagctca
     3541 tggatctgtg cttcacaaag agagaactca gttggcttga tgacctcatg ccagaaagta
     3601 agaaaaagaa agaagatgac aagaagaaga aggagaagga ggaagctgag cggatgcttc
     3661 agggtgacgg ggatactgtg cacctcccat tcgaaagggg gagtctccta caaattccag
     3721 ttaagaccct aaaatatagt attgaccctt cagttgttaa catatcagat gaaatggcca
     3781 aaactgccca gtggaaggca ctttccatga atactgagaa tgccaaagta accagaccta
     3841 acatgagccc tgaaaagcct gtgagtgtga caataaattt cgaagatgaa ccatcaaaaa
     3901 aatacatgga tgctgaaact tcattgtaaa gtcaaaccaa aaggaatata tagagatata
     3961 tgcctatctg tgtgtatgca caaacacaca cacacacaca cacacacaca cacgcacgca
     4021 cgcacgcacg cacacattta tatgtatgaa aagcacatgt gccatgcagt taatgtgtaa
     4081 cagtactttg gggctttttc aagtagcatc agatgtttgt tgtgagcacc atggagactg
     4141 cattaatgaa gtggtctcta gaagcaaggt acatgaattt ttactccaga tatttttttg
     4201 gagagtaaag cctttctgtt ttgcaataga aagatgtgga gaatgtgttc aatctgcttt
     4261 tcttaaatct ctgtcagtgg caatcaatca tatcaacctt aagtgataca tacttagtct
     4321 atatacttaa agtttcgacc tggatttcag aaggctttgc ttcccttttg gtagtgttca
     4381 taggtagaat aaggagcctc ccatgtggtg aaaatgacac gtcctgcctt ctctgtgggc
     4441 tttcttcatg taactgtcca tcatacttca caggtggtga acatagaggt gattagaagt
     4501 tagcagagac tgtctaggag ggaactttat gttttagaag ttatttttaa attttaaaac
     4561 tttcatatct gagcttccat ttagatggta cattaaaaaa attgatgtca cttcaattta
     4621 agacccactc aagtcataac gttttgagtt ttcaaaccta tgaagtgccc atttacagtg
     4681 gttaaatttt gttacctttg tggcaagcct tttccagtac tcagtacttg cctacaaacc
     4741 agagtcccac tgttctttgt cttcgttctt tacgtaggtg gtcaggttca ggtatctttc
     4801 tgttgtaagt agtaataaag agtcagcacc ctttcaagta ggcttgtgat caatgttcct
     4861 ttataagaat tatatttcat tttctgtgct tatttttcta ctatataaaa tgtatttaat
     4921 tggttagtat ccataatttc cttttaagta ttttatttat tataattgat gtgatactta
     4981 agtgtataaa tatttgagac agtaacaaac acagagtaca ttgcacctgt tgtagatgtt
     5041 tgtgctcact tgcatttctg ttcagtacct gaaagcaggt ggcaaactct acatccatag
     5101 tatagactac tgccatatta cattttcaaa ggaagttgga tatttcctgt acagctcaga
     5161 tccaatggca ttttgtcctg ttctgtgaga gcactgagtt tccaatactt ctgagctgtt
     5221 tcttaagcat gtggcaaata ttcttagctc cccacagagt aagtaaatgc tgttttaacc
     5281 tttgggacag aatgtttggg gccaggaaac ttgatttggc aaaggatagg taacacgtag
     5341 aaatggggag aaaccccaga agcactgaag gtgatggaac atagaggttt gggaaaatgt
     5401 cttaagggaa ttctagtgtt ctctagccat tgcaaactaa attacagaag taggaagaaa
     5461 ttctatgcat ctttagatca catgcataga attgcccaaa gtggattatt tttagcaact
     5521 ttgaaatcat tttagtttat tctaaacaat gtaccaatta gtgtaattcc ctcatctgag
     5581 atcacatagt tggattcttt ttaaaatagt agttggcaat atctagtact atctgtggag
     5641 ttctattgga cttcttagca tatgatggga gtgtcgccat ggtgcttaca gtcataggtg
     5701 acctccattg aagtggttcc ttttcattca gcagcacatt ggtttctgaa gtttctgttt
     5761 gctgctaatt ataacccatc ccacccccga tgtacccaaa catttccttg tgtcaggaca
     5821 tcaaagggtt aaatgtattt tacaggaagt gactttaaaa tttaaactac aaattacctg
     5881 gtatcataat cttcctgtga cttattaaat tatatgtgca ttatccagag tgggggcggg
     5941 ggcatgacgt acagaatact ttgcatctaa gatgcttcac tgtggttttg gttattgatg
     6001 ctttctgctt tcttttgact acttgactta caaagtgatc tgatgcgacc actccactga
     6061 agagcagggt aactgactgc ttccttcagg tggtcacatt tctcgtgcta ttttcgaaaa
     6121 taacaatcat gaataacatt cattgcaaga cagcaagaga gataatttag tatatatatt
     6181 gattttttta aatgctgcct taaataggtg tatatgagca gtagtaattg ctatgtactg
     6241 atttacctca aggtgcaaaa taattaaact tgtacatacc catttacaga ataagttcag
     6301 agacacagcc ctgcactttc tagatgcctt gattttcaga tggagcttag tgctactgaa
     6361 caccatggcc atggggccac ctcaggacgt tcttccctcc accatatttt atttatttat
     6421 agatgtgtgt ttacaaagac tgtaatgaag cctggtgtta actatctgaa acttacttcc
     6481 tttctcttgc tatttcaatc taaagtagga gcctttgaga aaacagtaaa ctagcttccc
     6541 aatgctaaag gggtgattct atatatattt ttaataatat tagatacgca aagtgaagta
     6601 caaagtcttt aaaatattgt gtgagtgttt gtgtatgtgt atgaaatcac tctttaactc
     6661 tcttaacttt tttaaaactc gtggtgtacc aatgttcaga tttgccaagc tttccctaag
     6721 acacgtagga tgaaatgttt ttgcgtgtta atgcatgtat ttgtaaagac ttgtttactg
     6781 ctgaaaatca ctgactcttg tttccatccc caaagtgttt gttccttttg catactggaa
     6841 ggttggattg agcataagct ttctggcttt cctttcacaa aggcaggttc aaaattgtgc
     6901 agacagaaga atggctgctg gcggtgagct gtggcatcag aactgcctgt agtgtcacca
     6961 gtgtgggcag tgtcaccagt gtaggcagtg tcaccagtgt gggcagtgcc agcattgtgg
     7021 gaagctcagc agcttcaagt ctgcgacctg accaagtgct cagagtggga tgtgggagcc
     7081 attaacagag cattgtaatt cagatggtta tttaagtaac ttccatgtct gaaatttatt
     7141 tatgttatca tttgtagcac aaatacatga actgtcttca tttagtccaa caattattta
     7201 tttttagaaa gtaaattgaa agacaagctg cattcaactt ttaaaatagt gttctaatca
     7261 caagatgtga tcatttaaat tgtaaggtct cttttaactt cctggaaagt agattgtcaa
     7321 aaggaatcat gtaaagatta ttttaaaatt ttaactgtat gtagttagag tgtaaacccc
     7381 agctctgtgg tctctaatca tattttttgc acattcattg tttctgttct gtagcttttc
     7441 ttgtattcct ttaagatatt tggcattgta ggggtaaggt gtcttccagt gtttactatg
     7501 ttaagcttaa attaaaattt aaatttattg tccatttata taatatttga tactttggtg
     7561 taacttctca atacagttac aatttttatg acatttataa ttgcaatcgt attttctttt
     7621 gtaataaaat ccaaagtcaa ttaaacaaat tgtagacctg gtatttctca tctatatgaa
     7681 gtacaagtct ctacctgagt ctataatgtg aaccatcctg cctttcacat ttgtttccta
     7741 attgttagag aaaactattt tttgtagatg ttattgaaag agcaataaaa gtctacatag
     7801 caa
//
