LOCUS       NM_031738               1678 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Rattus norvegicus solute carrier family 29 member 2 (Slc29a2),
            mRNA.
ACCESSION   NM_031738
VERSION     NM_031738.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Klein,D.M., Harding,M.C., Crowther,M.K. and Cherrington,N.J.
  TITLE     Localization of nucleoside transporters in rat epididymis
  JOURNAL   J Biochem Mol Toxicol 31 (8) (2017)
   PUBMED   28322028
  REMARK    GeneRIF: The purpose of this study is to determine the localization
            of nucleoside transporters expressed within rat epididymis to
            demonstrate the potential of epididymal reabsorption. Using
            immunohistochemistry, we determined that equilibrative nucleoside
            transporter 1 (ENT1) is localized to the basolateral membrane of
            epithelial cells, ENT2 is expressed in the nucleus of the
            epithelium and CNT2 is expressed by basal cells.
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Takagi,A., Nishimura,T., Akashi,T., Tomi,M. and Nakashima,E.
  TITLE     Contribution of equilibrative nucleoside transporter (ENT) 2 to
            fluorouracil transport in rat placental trophoblast cells
  JOURNAL   Drug Metab Pharmacokinet 32 (2), 151-156 (2017)
   PUBMED   28209435
  REMARK    GeneRIF: The ENT2 appears to be the mediator of fluorouracil
            transport in rat placental trophoblast cells.
REFERENCE   3  (bases 1 to 1678)
  AUTHORS   Klein,D.M., Evans,K.K., Hardwick,R.N., Dantzler,W.H., Wright,S.H.
            and Cherrington,N.J.
  TITLE     Basolateral uptake of nucleosides by Sertoli cells is mediated
            primarily by equilibrative nucleoside transporter 1
  JOURNAL   J Pharmacol Exp Ther 346 (1), 121-129 (2013)
   PUBMED   23639800
REFERENCE   4  (bases 1 to 1678)
  AUTHORS   Gilchrist,S.E. and Alcorn,J.
  TITLE     Lactation stage-dependent expression of transporters in rat whole
            mammary gland and primary mammary epithelial organoids
  JOURNAL   Fundam Clin Pharmacol 24 (2), 205-214 (2010)
   PUBMED   19702690
REFERENCE   5  (bases 1 to 1678)
  AUTHORS   Sato,K., Sai,Y., Nishimura,T., Chishu,T., Shimpo,S., Kose,N. and
            Nakashima,E.
  TITLE     Influx mechanism of 2',3'-dideoxyinosine and uridine at the
            blood-placenta barrier
  JOURNAL   Placenta 30 (3), 263-269 (2009)
   PUBMED   19135251
REFERENCE   6  (bases 1 to 1678)
  AUTHORS   Mangravite,L.M., Xiao,G. and Giacomini,K.M.
  TITLE     Localization of human equilibrative nucleoside transporters, hENT1
            and hENT2, in renal epithelial cells
  JOURNAL   Am J Physiol Renal Physiol 284 (5), F902-F910 (2003)
   PUBMED   12527552
REFERENCE   7  (bases 1 to 1678)
  AUTHORS   Yao,S.Y., Ng,A.M., Vickers,M.F., Sundaram,M., Cass,C.E.,
            Baldwin,S.A. and Young,J.D.
  TITLE     Functional and molecular characterization of nucleobase transport
            by recombinant human and rat equilibrative nucleoside transporters
            1 and 2. Chimeric constructs reveal a role for the ENT2 helix 5-6
            region in nucleobase translocation
  JOURNAL   J Biol Chem 277 (28), 24938-24948 (2002)
   PUBMED   12006583
REFERENCE   8  (bases 1 to 1678)
  AUTHORS   Kiss,A., Farah,K., Kim,J., Garriock,R.J., Drysdale,T.A. and
            Hammond,J.R.
  TITLE     Molecular cloning and functional characterization of
            inhibitor-sensitive (mENT1) and inhibitor-resistant (mENT2)
            equilibrative nucleoside transporters from mouse brain
  JOURNAL   Biochem J 352 Pt 2 (Pt 2), 363-372 (2000)
   PUBMED   11085929
REFERENCE   9  (bases 1 to 1678)
  AUTHORS   Griffiths,M., Yao,S.Y., Abidi,F., Phillips,S.E., Cass,C.E.,
            Young,J.D. and Baldwin,S.A.
  TITLE     Molecular cloning and characterization of a
            nitrobenzylthioinosine-insensitive (ei) equilibrative nucleoside
            transporter from human placenta
  JOURNAL   Biochem J 328 (Pt 3) (Pt 3), 739-743 (1997)
   PUBMED   9396714
REFERENCE   10 (bases 1 to 1678)
  AUTHORS   Yao,S.Y., Ng,A.M., Muzyka,W.R., Griffiths,M., Cass,C.E.,
            Baldwin,S.A. and Young,J.D.
  TITLE     Molecular cloning and functional characterization of
            nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive
            (ei) equilibrative nucleoside transporter proteins (rENT1 and
            rENT2) from rat tissues
  JOURNAL   J Biol Chem 272 (45), 28423-28430 (1997)
   PUBMED   9353301
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF015305.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF015305.1, SRR8487227.13178.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     gene            1..1678
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /note="solute carrier family 29 member 2"
                     /db_xref="GeneID:65194"
                     /db_xref="RGD:69296"
     exon            1..186
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..1528
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /note="nucleoside transporter, ei-type; solute carrier
                     family 29 (equilibrative nucleoside transporter), member
                     2; equilibrative NBMPR-insensitive nucleoside transporter;
                     equilibrative nitrobenzylmercaptopurine
                     riboside-insensitive nucleoside transporter; solute
                     carrier family 29 (nucleoside transporters), member 2"
                     /codon_start=1
                     /product="equilibrative nucleoside transporter 2"
                     /protein_id="NP_113926.1"
                     /db_xref="GeneID:65194"
                     /db_xref="RGD:69296"
                     /translation="MAHGNAPRDSYHLVGISFFILGLGTLLPWNFFITAIPYFQGRLA
                     GTNSSAETPSTNHTSPTDTFNFNNWVTLLSQLPLLLFTLLNSFLYQCIPESVRILGSL
                     LAILLLFALTAALVKVDLSPGLFFSITMASVWFINSFCAVLQGSLFGQLGTMPSTYST
                     LFLSGQGLAGIFAALAMLTSLASGVDPQTSALGYFITPCVGILLSIICYLSLPHLKFA
                     RYYLTKKPQAPVQELETKAELLGADEKNGIPVSPQQAGPTLDLDPEKELELGLEEPQK
                     PGKPSVFVVFRKIWLTALCLVLVFTVTLSVFPAITAMVTTSSNSPGKWSQFFNPICCF
                     LLFNVMDWLGRSLTSYFLWPDEDSQLLPLLVCLRFLFVPLFMLCHVPQRARLPIIFWQ
                     DAYFITFMLLFAISNGYFVSLTMCLAPRQVLPHEREVAGALMTFFLALGLSCGASLSF
                     LFKALL"
     exon            187..268
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
     exon            269..429
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
     exon            430..569
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
     exon            570..704
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
     exon            705..802
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
     exon            803..884
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1021
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1130
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1216
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1416
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1678
                     /gene="Slc29a2"
                     /gene_synonym="rENT2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttacccaacc tgcaccctct cactggctac agcactccct ccgaccttgg gattcccaga
       61 caccggccag cgttgctgcc cttcggcttc ctagccctgc gtaggcgcag gtgcaacctc
      121 cctgcagtgg gcctggtgca ataggactgc ggacatcatg gcgcacggaa acgcccctcg
      181 ggacagctac cacctggtcg ggatcagctt cttcattttg ggtctgggca ccctcctccc
      241 ctggaacttc ttcattaccg ccatcccgta cttccagggg cggttggcag ggaccaacag
      301 cagcgctgag accccgagca ccaaccacac cagtcccaca gacaccttca acttcaacaa
      361 ctgggtgaca ctgctgtccc agctgcctct actgctcttc acgctcctca actccttctt
      421 gtatcagtgc atccctgagt cggtgcgtat tctgggcagc ctgctggcca tactactgct
      481 ctttgccctg acagcagcgc tggtgaaggt ggatttgagc cccgggctgt tcttttccat
      541 caccatggca tccgtctggt tcatcaattc cttctgtgca gtgcttcaag gcagcctctt
      601 tgggcagctg ggtaccatgc cttctaccta cagcaccctc ttcctcagtg gccagggcct
      661 ggctgggatc ttcgctgccc ttgctatgct cacgtcctta gccagtggcg tggaccccca
      721 gacctctgcc cttgggtact tcatcacacc ctgtgtgggc atcctcctct ccatcatatg
      781 ttacctcagt ctgccccatc tgaagtttgc ccgttactac ctgaccaaga agcctcaggc
      841 cccagttcag gagctggaga ccaaagctga gctcctcgga gctgatgaga agaatgggat
      901 tcctgtcagc ccccagcagg caggcccaac tctggatctt gacccagaga aggagctgga
      961 gctggggctg gaggaaccac agaagccagg aaaaccttcg gtctttgtcg tcttccggaa
     1021 gatctggctg acggcgctgt gccttgtgtt ggtcttcaca gtcaccctgt cggtctttcc
     1081 tgctatcaca gccatggtga ccaccagctc caatagcccc gggaagtgga gtcagttctt
     1141 caaccctatc tgttgcttcc tgctcttcaa cgtcatggat tggctgggcc ggagcctgac
     1201 ttcctacttc ctgtggccag atgaggacag ccagctgctg cccctgctgg tgtgcctgcg
     1261 cttcctgttc gtgcccctct tcatgctgtg tcatgtgccc cagcgtgccc ggctgcccat
     1321 catcttctgg caggatgcct acttcatcac attcatgctg ctcttcgcca tttccaacgg
     1381 ctacttcgtg tctctcacca tgtgtctggc acccaggcag gtgctgccgc atgagaggga
     1441 ggtggccgga gccctcatga ccttcttcct ggccctggga ctctcctgcg gagcctctct
     1501 ctccttcctc tttaaggctt tactctgagg accaccgcac tccacagtcc tccctcactg
     1561 ctgcttctgg agctggacat tcccggggaa ggacctctgc tataccgggt ctcttggtct
     1621 gtgggtgcca ggagggggca gaaactacct tggctggtag ccacattgca tatggtga
//
