LOCUS       NM_031664               2876 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus solute carrier family 28 member 2 (Slc28a2),
            mRNA.
ACCESSION   NM_031664 XM_345427
VERSION     NM_031664.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Klein,D.M., Harding,M.C., Crowther,M.K. and Cherrington,N.J.
  TITLE     Localization of nucleoside transporters in rat epididymis
  JOURNAL   J Biochem Mol Toxicol 31 (8) (2017)
   PUBMED   28322028
  REMARK    GeneRIF: The purpose of this study is to determine the localization
            of nucleoside transporters expressed within rat epididymis to
            demonstrate the potential of epididymal reabsorption. Using
            immunohistochemistry, we determined that equilibrative nucleoside
            transporter 1 (ENT1) is localized to the basolateral membrane of
            epithelial cells, ENT2 is expressed in the nucleus of the
            epithelium and CNT2 is expressed by basal cells.
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Pinilla-Macua,I., Claudio-Montero,A. and Pastor-Anglada,M.
  TITLE     rCNT2 extracellular cysteines, Cys(615) and Cys(649), are important
            for maturation and sorting to the plasma membrane
  JOURNAL   FEBS Lett 588 (23), 4382-4389 (2014)
   PUBMED   25315414
  REMARK    GeneRIF: rCNT2 extracellular cysteines, Cys(615) and Cys(649), are
            important for maturation and sorting to the plasma membrane
REFERENCE   3  (bases 1 to 2876)
  AUTHORS   Medina-Pulido,L., Molina-Arcas,M., Justicia,C., Soriano,E.,
            Burgaya,F., Planas,A.M. and Pastor-Anglada,M.
  TITLE     Hypoxia and P1 receptor activation regulate the high-affinity
            concentrative adenosine transporter CNT2 in differentiated neuronal
            PC12 cells
  JOURNAL   Biochem J 454 (3), 437-445 (2013)
   PUBMED   23819782
  REMARK    GeneRIF: CNT2 is likely to modulate extracellular adenosine and
            cell energy balance in neuronal tissue.
REFERENCE   4  (bases 1 to 2876)
  AUTHORS   Pinilla-Macua,I., Casado,F.J. and Pastor-Anglada,M.
  TITLE     Structural determinants for rCNT2 sorting to the plasma membrane of
            polarized and non-polarized cells
  JOURNAL   Biochem J 442 (3), 517-525 (2012)
   PUBMED   22136384
  REMARK    GeneRIF: the N-terminal tail of rCNT2 contains dual sorting
            signals. An acidic region is responsible for its proper
            stabilization at the plasma membrane, whereas the putative CK2
            domain (Ser(46)) is implicated in the apical sorting of the
            transporter.
REFERENCE   5  (bases 1 to 2876)
  AUTHORS   Nishimura,T., Chishu,T., Tomi,M., Nakamura,R., Sato,K., Kose,N.,
            Sai,Y. and Nakashima,E.
  TITLE     Mechanism of nucleoside uptake in rat placenta and induction of
            placental CNT2 in experimental diabetes
  JOURNAL   Drug Metab Pharmacokinet 27 (4), 439-446 (2012)
   PUBMED   22354287
  REMARK    GeneRIF: Report induction of CNT2 in experimental diabetes
            mellitus.
REFERENCE   6  (bases 1 to 2876)
  AUTHORS   Patel,D.H., Crawford,C.R., Naeve,C.W. and Belt,J.A.
  TITLE     Cloning, genomic organization and chromosomal localization of the
            gene encoding the murine sodium-dependent, purine-selective,
            concentrative nucleoside transporter (CNT2)
  JOURNAL   Gene 242 (1-2), 51-58 (2000)
   PUBMED   10721696
REFERENCE   7  (bases 1 to 2876)
  AUTHORS   Anderson,C.M., Xiong,W., Young,J.D., Cass,C.E. and Parkinson,F.E.
  TITLE     Demonstration of the existence of mRNAs encoding N1/cif and N2/cit
            sodium/nucleoside cotransporters in rat brain
  JOURNAL   Brain Res Mol Brain Res 42 (2), 358-361 (1996)
   PUBMED   9013795
REFERENCE   8  (bases 1 to 2876)
  AUTHORS   Yao,S.Y., Ng,A.M., Ritzel,M.W., Gati,W.P., Cass,C.E. and Young,J.D.
  TITLE     Transport of adenosine by recombinant purine- and
            pyrimidine-selective sodium/nucleoside cotransporters from rat
            jejunum expressed in Xenopus laevis oocytes
  JOURNAL   Mol Pharmacol 50 (6), 1529-1535 (1996)
   PUBMED   8967974
REFERENCE   9  (bases 1 to 2876)
  AUTHORS   Che,M., Ortiz,D.F. and Arias,I.M.
  TITLE     Primary structure and functional expression of a cDNA encoding the
            bile canalicular, purine-specific Na(+)-nucleoside cotransporter
  JOURNAL   J Biol Chem 270 (23), 13596-13599 (1995)
   PUBMED   7775409
REFERENCE   10 (bases 1 to 2876)
  AUTHORS   Che,M., Nishida,T., Gatmaitan,Z. and Arias,I.M.
  TITLE     A nucleoside transporter is functionally linked to
            ectonucleotidases in rat liver canalicular membrane
  JOURNAL   J Biol Chem 267 (14), 9684-9688 (1992)
   PUBMED   1315767
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On Feb 11, 2021 this sequence version replaced NM_031664.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY029302.1, U25055.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-249               JAXUCZ010000003.1  129819788-129820036
            250-346             JAXUCZ010000003.1  129820588-129820684
            347-438             JAXUCZ010000003.1  129820778-129820869
            439-530             JAXUCZ010000003.1  129828302-129828393
            531-714             JAXUCZ010000003.1  129829191-129829374
            715-856             JAXUCZ010000003.1  129831391-129831532
            857-970             JAXUCZ010000003.1  129832547-129832660
            971-1048            JAXUCZ010000003.1  129833045-129833122
            1049-1129           JAXUCZ010000003.1  129833252-129833332
            1130-1210           JAXUCZ010000003.1  129833752-129833832
            1211-1336           JAXUCZ010000003.1  129835756-129835881
            1337-1467           JAXUCZ010000003.1  129835963-129836093
            1468-1636           JAXUCZ010000003.1  129836495-129836663
            1637-1834           JAXUCZ010000003.1  129836830-129837027
            1835-1916           JAXUCZ010000003.1  129837587-129837668
            1917-2015           JAXUCZ010000003.1  129839010-129839108
            2016-2127           JAXUCZ010000003.1  129839282-129839393
            2128-2876           JAXUCZ010000003.1  129840544-129841292
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q35"
     gene            1..2876
                     /gene="Slc28a2"
                     /note="solute carrier family 28 member 2"
                     /db_xref="GeneID:60423"
                     /db_xref="RGD:61840"
     exon            1..249
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            250..346
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     CDS             266..2245
                     /gene="Slc28a2"
                     /note="SPNT; CNT 2; rCNT2; concentrative nucleoside
                     transporter 2; sodium/purine nucleoside cotransporter;
                     sodium-coupled nucleoside transporter 2; Na(+)/nucleoside
                     cotransporter 2; solute carrier family 28 (sodium-coupled
                     nucleoside transporter), member 2; solute carrier family
                     28 (concentrative nucleoside transporter), member 2;
                     sodium/nucleoside cotransporter 2-like"
                     /codon_start=1
                     /product="sodium/nucleoside cotransporter 2"
                     /protein_id="NP_113852.2"
                     /db_xref="GeneID:60423"
                     /db_xref="RGD:61840"
                     /translation="MAKSEGRKSASQDTSENGMENPGLELMEVGNLEQGKTLEEVTQG
                     HSLKDGLGHSSLWRRILQPFTKARSFYQRHAGLFKKILLGLLCLAYAAYLLAACILNF
                     RRAQALFVITCLVIFILACHFLKKFFAKKSIRCLKPLKNTRLRLWLKRVFMGAAVVGL
                     ILWLALDTAQRPEQLISFAGICMFILILFACSKHHSAVSWRTVFWGLGLQFVFGILVI
                     RTEPGFNAFQWLGDQIQIFLAYTVEGSSFVFGDTLVQSVFAFQSLPIIIFFGCVMSIL
                     YYLGLVQWVIQKIAWFLQITMGTTAAETLAVAGNIFVGMTEAPLLIRPYLADMTLSEI
                     HAVMTGGFATIAGTVLGAFISFGIDASSLISASVMAAPCALALSKLVYPEVEESKFKS
                     KEGVKLPRGEERNILEAASNGATDAIGLVANVAANLIAFLAVLAFINSTLSWLGEMVD
                     IHGLTFQVICSYVLRPMVFMMGVQWADCPLVAEIVGVKFFINEFVAYQQLSQYKNKRL
                     SGVEEWINGEKQWISVKAEIIATFSLCGFANLTSIGITLGGLTSMVPQRKSDLCKLVV
                     RALFTGACVSFISACMAGILYVPRGAETDCVSFLNTNFTNRTYETYVCCRELFQSTLL
                     NGTNMPSFSGPWQDKESSLRNLAKCCDLYTSTVCA"
     exon            347..438
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            439..530
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            531..714
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            715..856
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            857..970
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1048
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1129
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1210
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1336
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1467
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1636
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1834
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..1916
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2015
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2127
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2876
                     /gene="Slc28a2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagaagtctt gctgaaggga aacctccact tcctgcttgt gagagtatta atccctgctg
       61 cagctgtcca tccccactga catcagactc cagtttctcc aggatccagt gtgttctgaa
      121 gatcagcagc tctcccggag tcttcaaggc gccagatttg gaagtctgag gcatctggct
      181 ttgtaacctg agcagctgcc aggttccagc cattatctga ctgggctgtg cggacccacc
      241 cagcacattc agaggataac aggagatggc gaagtcagag ggaagaaagt ctgcttccca
      301 ggacacatcg gagaatggca tggagaaccc aggcctggag ctcatggaag tcggaaacct
      361 tgagcaaggc aaaacactgg aggaagtgac acagggacat agcctgaagg atggcttggg
      421 gcattcctct ctctggagga ggattcttca gcccttcacc aaggcgagaa gtttctatca
      481 aagacatgct ggtttattca aaaagatcct gcttggtctc ttgtgtttag cgtatgctgc
      541 ctacctcctg gcagcttgca tcctgaactt ccggagggca caggccttgt ttgtcatcac
      601 ctgccttgtc atcttcatcc tggcttgcca ctttctgaaa aaattctttg ctaaaaagtc
      661 aataagatgc ttgaagccct tgaaaaacac ccgcctgaga ctttggctta agagggtgtt
      721 catgggagcc gctgttgtcg gccttattct ctggctggct ctggacacag ctcaaaggcc
      781 agagcagctg atctcctttg cgggaatctg catgttcatc ctcatcctct ttgcctgctc
      841 caaacaccac agtgcggtga gctggaggac agtgttttgg ggcctgggcc ttcagttcgt
      901 ctttgggatt ttggtcatca gaactgaacc tggatttaat gcatttcaat ggctgggaga
      961 tcagatccag attttcctgg catatactgt agaaggatcc agttttgttt ttggtgacac
     1021 actggtccaa agtgtttttg cttttcagtc tctcccaatc attattttct tcggatgtgt
     1081 gatgtctatt ctctactacc tgggccttgt gcagtgggtg atccagaaga ttgcctggtt
     1141 cctgcaaatc accatgggta ccacagctgc agagaccctg gccgtggcag gaaacatctt
     1201 tgtgggaatg acagaggcac ctctgctcat ccgtccctac cttgcagaca tgaccctctc
     1261 tgaaatccat gcagtgatga ctggaggctt tgctactata gcaggcacag tgttgggagc
     1321 cttcatatcc tttgggattg atgcatcatc cttgatttct gcctcagtga tggctgcccc
     1381 ttgtgcactt gccttgtcca aactggtata tccagaagta gaagagtcca agttcaagag
     1441 caaggaggga gtgaagctgc ctcgagggga agagaggaat attctggaag ccgcgagcaa
     1501 cggagccaca gatgccatag gccttgttgc taatgttgca gccaacctga ttgcctttct
     1561 ggctgtattg gccttcatca attctaccct ttcctggctg ggggaaatgg tggatatcca
     1621 tggactcact ttccaggtca tctgctccta tgtcctaagg ccaatggttt tcatgatggg
     1681 tgtacagtgg gcagactgcc cattggtggc tgagatagtg ggagtcaaat tcttcataaa
     1741 tgaatttgtg gcctatcaac aactgtctca gtacaagaac aaacgtctct ctggagtcga
     1801 agagtggatc aatggcgaga aacagtggat ttctgtgaag gctgaaatca ttgcaacatt
     1861 ttctctctgt ggatttgcca atcttacttc catagggatc acactgggag gcttgacatc
     1921 catggttccc caacggaaga gtgacttgtg caagcttgtg gtcagggccc tcttcacagg
     1981 tgcctgtgtt tccttcatca gtgcctgtat ggcaggaatc ctctatgttc ccagaggcgc
     2041 tgaaactgac tgtgtctcat tcttaaacac aaatttcacc aacagaacct atgagacata
     2101 tgtgtgctgc agagagctct tccagagtac tttgctgaat ggcaccaaca tgccttcttt
     2161 ctcaggcccc tggcaagaca aggagtccag cctcaggaat cttgctaaat gctgtgacct
     2221 ctataccagc actgtgtgtg cctgaggctg gtggggccat tccgtgacag ttcttataga
     2281 acagattcgt attaccaaat acatgttcat gtacttaggc ttattctctg agaactgcac
     2341 taaactccac aaaatgaaag tcctgatctc actgtggctc tgtataaaaa ccagcatcca
     2401 cctttccact ggcatgccac atagtagatg tgaaattcat tatagaccct cttgagccaa
     2461 agtatggtgg gaatgtagct aagggggaaa tatgtgtgtg gtaagtgcta agatggagat
     2521 tcaacccctg gccagggcca gagtggggag agtggggaaa tccaagtaga tgcttagatt
     2581 taacttggtg gtacacccct gtggtcactt acctgtaagc tcagagctaa ggggattgcc
     2641 tcagatccaa ggcaagcctg gcccgaaccc tgaagtcttt cacagaagtg atagagtaga
     2701 ggccatgtcc ttgggcaggg atggaggtga gggaatatat ttctaaagtt atatgggaaa
     2761 cacttgtgaa tacttaatga taacatattt tttattgcca aaggttgtat acttaaaaac
     2821 tcttttcatt ttcatacatt actaaaaagc tttctgtaat aaagtgtttt tatgta
//
