LOCUS       NM_053965               1791 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus solute carrier family 25 member 20 (Slc25a20),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_053965
VERSION     NM_053965.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Tonazzi,A., Giangregorio,N., Console,L., De Palma,A. and
            Indiveri,C.
  TITLE     Nitric oxide inhibits the mitochondrial carnitine/acylcarnitine
            carrier through reversible S-nitrosylation of cysteine 136
  JOURNAL   Biochim Biophys Acta Bioenerg 1858 (7), 475-482 (2017)
   PUBMED   28438511
  REMARK    GeneRIF: Inhibition by the NO-releasing compound GSNO suggests that
            C136 S-nitrosylation of native and recombinant CACT may have a role
            in the regulation of the beta-oxidation pathway.
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Wilson,R., Geyer,S.H., Reissig,L., Rose,J., Szumska,D., Hardman,E.,
            Prin,F., McGuire,C., Ramirez-Solis,R., White,J., Galli,A.,
            Tudor,C., Tuck,E., Mazzeo,C.I., Smith,J.C., Robertson,E.,
            Adams,D.J., Mohun,T. and Weninger,W.J.
  TITLE     Highly variable penetrance of abnormal phenotypes in embryonic
            lethal knockout mice
  JOURNAL   Wellcome Open Res 1, 1 (2017)
   PUBMED   27996060
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1791)
  AUTHORS   Giangregorio,N., Tonazzi,A., Console,L. and Indiveri,C.
  TITLE     Post-translational modification by acetylation regulates the
            mitochondrial carnitine/acylcarnitine transport protein
  JOURNAL   Mol Cell Biochem 426 (1-2), 65-73 (2017)
   PUBMED   27864727
  REMARK    GeneRIF: The experimental data indicate that acetylation of CACT
            inhibits its transport activity, and thus may contribute to the
            regulation of the mitochondrial beta-oxidation pathway.
REFERENCE   4  (bases 1 to 1791)
  AUTHORS   Console,L., Giangregorio,N., Indiveri,C. and Tonazzi,A.
  TITLE     Carnitine/acylcarnitine translocase and carnitine
            palmitoyltransferase 2 form a complex in the inner mitochondrial
            membrane
  JOURNAL   Mol Cell Biochem 394 (1-2), 307-314 (2014)
   PUBMED   24898781
  REMARK    GeneRIF: Report interaction between carnitine palmitoyltransferase
            2 and carnitine/acylcarnitine translocase supporting the channeling
            of acylcarnitines and carnitine at level of the inner mitochondrial
            membrane.
            Erratum:[Mol Cell Biochem. 2014 Sep;394(1-2):315]
REFERENCE   5  (bases 1 to 1791)
  AUTHORS   Tonazzi,A., Console,L., Giangregorio,N., Indiveri,C. and
            Palmieri,F.
  TITLE     Identification by site-directed mutagenesis of a hydrophobic
            binding site of the mitochondrial carnitine/acylcarnitine carrier
            involved in the interaction with acyl groups
  JOURNAL   Biochim Biophys Acta 1817 (5), 697-704 (2012)
   PUBMED   22365929
  REMARK    GeneRIF: The data demonstrated that V25, P78, V82, M85 and C89 are
            involved in the acyl chain binding to the CAC in c-state.
REFERENCE   6  (bases 1 to 1791)
  AUTHORS   Indiveri,C., Giangregorio,N., Iacobazzi,V. and Palmieri,F.
  TITLE     Site-directed mutagenesis and chemical modification of the six
            native cysteine residues of the rat mitochondrial carnitine
            carrier: implications for the role of cysteine-136
  JOURNAL   Biochemistry 41 (27), 8649-8656 (2002)
   PUBMED   12093282
  REMARK    GeneRIF: Site-directed mutagenesis and chemical modification of the
            six native cysteine residues: implications for the role of
            cysteine-136
REFERENCE   7  (bases 1 to 1791)
  AUTHORS   Indiveri,C., Iacobazzi,V., Giangregorio,N. and Palmieri,F.
  TITLE     Bacterial overexpression, purification, and reconstitution of the
            carnitine/acylcarnitine carrier from rat liver mitochondria
  JOURNAL   Biochem Biophys Res Commun 249 (3), 589-594 (1998)
   PUBMED   9731180
REFERENCE   8  (bases 1 to 1791)
  AUTHORS   Huizing,M., Iacobazzi,V., Ijlst,L., Savelkoul,P., Ruitenbeek,W.,
            van den Heuvel,L., Indiveri,C., Smeitink,J., Trijbels,F.,
            Wanders,R. and Palmieri,F.
  TITLE     Cloning of the human carnitine-acylcarnitine carrier cDNA and
            identification of the molecular defect in a patient
  JOURNAL   Am J Hum Genet 61 (6), 1239-1245 (1997)
   PUBMED   9399886
REFERENCE   9  (bases 1 to 1791)
  AUTHORS   Indiveri,C., Iacobazzi,V., Giangregorio,N. and Palmieri,F.
  TITLE     The mitochondrial carnitine carrier protein: cDNA cloning, primary
            structure and comparison with other mitochondrial transport
            proteins
  JOURNAL   Biochem J 321 (Pt 3) (Pt 3), 713-719 (1997)
   PUBMED   9032458
REFERENCE   10 (bases 1 to 1791)
  AUTHORS   Indiveri,C., Tonazzi,A. and Palmieri,F.
  TITLE     Identification and purification of the carnitine carrier from rat
            liver mitochondria
  JOURNAL   Biochim Biophys Acta 1020 (1), 81-86 (1990)
   PUBMED   2223786
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC081749.1.
            
            On Sep 16, 2004 this sequence version replaced NM_053965.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081749.1, FQ209681.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q32"
     gene            1..1791
                     /gene="Slc25a20"
                     /gene_synonym="CACT"
                     /note="solute carrier family 25 member 20"
                     /db_xref="GeneID:117035"
                     /db_xref="RGD:621443"
     exon            1..205
                     /gene="Slc25a20"
                     /gene_synonym="CACT"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..1006
                     /gene="Slc25a20"
                     /gene_synonym="CACT"
                     /EC_number="2.7.11.1"
                     /note="CAC; carnitine/acylcarnitine translocase; solute
                     carrier family 25 (mitochondrial carnitine/acylcarnitine
                     translocase), member 20; solute carrier family 25
                     (carnitine/acylcarnitine translocase), member 20"
                     /codon_start=1
                     /product="mitochondrial carnitine/acylcarnitine carrier
                     protein"
                     /protein_id="NP_446417.2"
                     /db_xref="GeneID:117035"
                     /db_xref="RGD:621443"
                     /translation="MAEEPKPISPLKNLLAGGFGGVCLVFVGHPLDTVKVRLQTQPPS
                     LPGQPPMYSGTIDCFRKTLFREGITGLYRGMAAPIIGVTPMFAVCFFGFGLGKRLQQK
                     SPEDELTYPQLFTAGMLSGVFTTGIMTPGERIKCLLQIQASSGKNKYSGTLDCAKKLY
                     QEFGIRGFYKGTVLTLMRDVPASGMYFMTYEWLKNLFTPQGKSVHDLSVPRVLVAGGF
                     AGIFNWVVAIPPDVLKSRFQTAPPGKYPNGFRDVLRELIREEGVTSLYKGFNAVMIRA
                     FPANAACFLGFEIAMKILNWIAPNL"
     exon            206..298
                     /gene="Slc25a20"
                     /gene_synonym="CACT"
                     /inference="alignment:Splign:2.1.0"
     exon            299..426
                     /gene="Slc25a20"
                     /gene_synonym="CACT"
                     /inference="alignment:Splign:2.1.0"
     exon            427..517
                     /gene="Slc25a20"
                     /gene_synonym="CACT"
                     /inference="alignment:Splign:2.1.0"
     exon            518..635
                     /gene="Slc25a20"
                     /gene_synonym="CACT"
                     /inference="alignment:Splign:2.1.0"
     exon            636..708
                     /gene="Slc25a20"
                     /gene_synonym="CACT"
                     /inference="alignment:Splign:2.1.0"
     exon            709..818
                     /gene="Slc25a20"
                     /gene_synonym="CACT"
                     /inference="alignment:Splign:2.1.0"
     exon            819..943
                     /gene="Slc25a20"
                     /gene_synonym="CACT"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1754
                     /gene="Slc25a20"
                     /gene_synonym="CACT"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcccacctt aatgtaggtg aaaggtcggt gtagccgctt ggacagcgga gcaagatcaa
       61 gactgccagg agctgcaagt agactgacag aagactgacc atggcagagg agccgaaacc
      121 catcagtccg cttaagaacc tccttgccgg cggctttggc ggcgtgtgcc tggtgtttgt
      181 ggggcacccc ttggacacgg tcaaggtccg actgcagaca caaccaccaa gtttgcctgg
      241 gcagccacct atgtactctg ggaccatcga ctgtttccgg aagactcttt ttagagaggg
      301 catcacaggg ctgtatcggg gcatggctgc acccatcatt ggagtcaccc ctatgtttgc
      361 cgtgtgcttc tttgggtttg gtttggggaa gagactacag cagaaatctc cagaggatga
      421 acttacctac ccgcagctgt ttacagctgg gatgttatct ggtgtgttca ccacaggaat
      481 catgacccct ggagaacgga tcaaatgctt actgcagatt caggcttctt cagggaaaaa
      541 caaatacagt ggtaccttgg actgtgcaaa gaagctgtat caggagtttg ggatccgtgg
      601 cttctacaaa gggactgtgc tcactctcat gcgagatgtt cctgccagtg ggatgtattt
      661 catgacatac gaatggctta aaaatctctt cactccacag ggaaagagtg tccatgacct
      721 cagtgtgcct cgggtcctgg tggctggcgg ctttgcaggg atcttcaatt gggttgtggc
      781 aatcccccca gacgtgctca agtctcgatt ccagaccgca cctcctggaa aatatcctaa
      841 tggtttcaga gatgtgttga gagagctgat ccgagaagaa ggagtcacct ccttgtacaa
      901 agggttcaat gcagtcatga tccgagcctt cccagccaat gctgcctgtt tccttggctt
      961 tgaaattgcc atgaagatcc tcaattggat tgcccccaac ttgtgaggct gaagactgtt
     1021 gaagaccttt ggatgctgga agactgttgc cgagaagcag gggcaggact gggaagccct
     1081 gagggtgagg ggagggaagt ggtgtgatca gagccccagg catgactgtg atggtgaaac
     1141 tgttgcctta atgacatcct ctaccttatg taacttggtg ccattttgaa acttgaatat
     1201 agaaggtttc cagagaattg gagatggaat aaatagttta tagactcgct acctttgacc
     1261 aaattgccac ctactggttg actgaaggcc ctacttacta tactcaaata ctccttacca
     1321 aagagttgat ggctgactct ctggatgccc cttgcctatt ctcaaccagc taacccagga
     1381 gctggaatct ttaattgcta gccatgaaaa cccaagagat gtccacagta ccatctactc
     1441 ctggccatac acagggatgt ggatgtgaag cacacatctg tctgcccaaa ggactaggca
     1501 gacaatacct aaggttagcc cacctgttat ccactggttt ttgatatttt tacacaagaa
     1561 atactaataa taaaatgaca attgtctaaa ccccctggag aaggtggaga attttccctc
     1621 ctacacagtg aagacagccc cagcctcctg ggataagtgt gaaagccaca atgtaggaag
     1681 gctctttttg cacattcttc ccccatgaga gcactcagtt ttaacaggaa acaataaata
     1741 ttgtttctaa ttttaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//
