LOCUS       NM_031332               2157 bp    mRNA    linear   ROD 31-MAY-2024
DEFINITION  Rattus norvegicus solute carrier family 22 member 8 (Slc22a8),
            mRNA.
ACCESSION   NM_031332
VERSION     NM_031332.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Taniguchi,T., Omura,K., Motoki,K., Sakai,M., Chikamatsu,N.,
            Ashizawa,N., Takada,T. and Iwanaga,T.
  TITLE     Hypouricemic agents reduce indoxyl sulfate excretion by inhibiting
            the renal transporters OAT1/3 and ABCG2
  JOURNAL   Sci Rep 11 (1), 7232 (2021)
   PUBMED   33790363
  REMARK    GeneRIF: Hypouricemic agents reduce indoxyl sulfate excretion by
            inhibiting the renal transporters OAT1/3 and ABCG2.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Pengrattanachot,N., Cherngwelling,R., Jaikumkao,K.,
            Pongchaidecha,A., Thongnak,L., Swe,M.T., Chatsudthipong,V. and
            Lungkaphin,A.
  TITLE     Atorvastatin attenuates obese-induced kidney injury and impaired
            renal organic anion transporter 3 function through inhibition of
            oxidative stress and inflammation
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1866 (6), 165741 (2020)
   PUBMED   32101757
  REMARK    GeneRIF: Atorvastatin attenuates obese-induced kidney injury and
            impaired renal organic anion transporter 3 function through
            inhibition of oxidative stress and inflammation.
REFERENCE   3  (bases 1 to 2157)
  AUTHORS   Bush,K.T., Singh,P. and Nigam,S.K.
  TITLE     Gut-derived uremic toxin handling in vivo requires OAT-mediated
            tubular secretion in chronic kidney disease
  JOURNAL   JCI Insight 5 (7), 133817 (2020)
   PUBMED   32271169
  REMARK    GeneRIF: Gut-derived uremic toxin handling in vivo requires
            OAT-mediated tubular secretion in chronic kidney disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2157)
  AUTHORS   Wanchai,K., Yasom,S., Tunapong,W., Chunchai,T.,
            Eaimworawuthikul,S., Thiennimitr,P., Chaiyasut,C.,
            Pongchaidecha,A., Chatsudthipong,V., Chattipakorn,S.,
            Chattipakorn,N. and Lungkaphin,A.
  TITLE     Probiotic Lactobacillus paracasei HII01 protects rats against
            obese-insulin resistance-induced kidney injury and impaired renal
            organic anion transporter 3 function
  JOURNAL   Clin Sci (Lond) 132 (14), 1545-1563 (2018)
   PUBMED   29980603
  REMARK    GeneRIF: Data suggest that a remote sensing and signaling system
            between gut and kidney by which probiotic might facilitate renal
            handling of gut microbiota products through the improvement of
            organic anion transporter 3 (Oat3) function.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2157)
  AUTHORS   Zhang,Z., Tachikawa,M., Uchida,Y. and Terasaki,T.
  TITLE     Drug Clearance from Cerebrospinal Fluid Mediated by Organic Anion
            Transporters 1 (Slc22a6) and 3 (Slc22a8) at Arachnoid Membrane of
            Rats
  JOURNAL   Mol Pharm 15 (3), 911-922 (2018)
   PUBMED   29436232
  REMARK    GeneRIF: Study results indicate that Oat1 and Oat3 at the
            blood-arachnoid barrier provide a distinct clearance pathway of
            organic anion drugs from cerebrospinal fluid independently of
            choroid plexus.
REFERENCE   6  (bases 1 to 2157)
  AUTHORS   Feng,B., Shu,Y. and Giacomini,K.M.
  TITLE     Role of aromatic transmembrane residues of the organic anion
            transporter, rOAT3, in substrate recognition
  JOURNAL   Biochemistry 41 (28), 8941-8947 (2002)
   PUBMED   12102636
  REMARK    GeneRIF: Role of aromatic transmembrane residues in substrate
            recognition
REFERENCE   7  (bases 1 to 2157)
  AUTHORS   Nagata,Y., Kusuhara,H., Endou,H. and Sugiyama,Y.
  TITLE     Expression and functional characterization of rat organic anion
            transporter 3 (rOat3) in the choroid plexus
  JOURNAL   Mol Pharmacol 61 (5), 982-988 (2002)
   PUBMED   11961115
REFERENCE   8  (bases 1 to 2157)
  AUTHORS   Hasegawa,M., Kusuhara,H., Sugiyama,D., Ito,K., Ueda,S., Endou,H.
            and Sugiyama,Y.
  TITLE     Functional involvement of rat organic anion transporter 3 (rOat3;
            Slc22a8) in the renal uptake of organic anions
  JOURNAL   J Pharmacol Exp Ther 300 (3), 746-753 (2002)
   PUBMED   11861777
REFERENCE   9  (bases 1 to 2157)
  AUTHORS   Kobayashi,Y., Hirokawa,N., Ohshiro,N., Sekine,T., Sasaki,T.,
            Tokuyama,S., Endou,H. and Yamamoto,T.
  TITLE     Differential gene expression of organic anion transporters in male
            and female rats
  JOURNAL   Biochem Biophys Res Commun 290 (1), 482-487 (2002)
   PUBMED   11779196
REFERENCE   10 (bases 1 to 2157)
  AUTHORS   Kusuhara,H., Sekine,T., Utsunomiya-Tate,N., Tsuda,M., Kojima,R.,
            Cha,S.H., Sugiyama,Y., Kanai,Y. and Endou,H.
  TITLE     Molecular cloning and characterization of a new multispecific
            organic anion transporter from rat brain
  JOURNAL   J Biol Chem 274 (19), 13675-13680 (1999)
   PUBMED   10224140
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB017446.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB017446.1, FQ209755.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     gene            1..2157
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /note="solute carrier family 22 member 8"
                     /db_xref="GeneID:83500"
                     /db_xref="RGD:632286"
     exon            1..99
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            100..456
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..1734
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /note="rOat3; solute carrier family 22 (organic anion
                     transporter), member 8; organic anion/dicarboxylate
                     exchanger"
                     /codon_start=1
                     /product="organic anion transporter 3"
                     /protein_id="NP_112622.1"
                     /db_xref="GeneID:83500"
                     /db_xref="RGD:632286"
                     /translation="MTFSEILDRVGSMGPFQYLHVTLLALPVLGIANHNLLQIFTATT
                     PVHHCRPPPNASIGPWVLPLDPNGKPEKCLRFVHLPNASLPNDTQRATEPCLDGWIYN
                     STRDTIVIEWDLVCSSNKLKEMAQSIFMAGILVGGPVIGELSDRFGRKPILTWSYLML
                     AASGSGAAFSPSLPVYMIFRFLCGCSISGISLSTVILNVEWVPTSMRAISSTSIGYCY
                     TIGQFILSGLAYAIPQWRWLQLTSSAPFFIFSLLSWWVPESIRWLVLSGKYSKALKTL
                     QRVATFNGKKEEGKKLTIEELKFNLQKDITSAKVKYGLSDLFRVSILRRVTFCLSLAW
                     FSTGFAYYSLAMGVEEFGVNIYILQIIFGGVDIPAKFITILSLSYLGRRITQSFLLLL
                     AGGAILALIFVPSEMQLLRTALAVFGKGCLSGSFSCLFLYTSELYPTVLRQTGMGISN
                     VWARVGSMIAPLVKITGELQPFIPNVIFGTTALLGGSAAFFLLETLNRPLPETIEDIQ
                     NWHKQVQKTKQESEAEKASQIIPLKTGG"
     exon            457..560
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            561..715
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            716..884
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1008
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1124
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1339
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1448
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1652
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..2157
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2157
                     /gene="Slc22a8"
                     /gene_synonym="Oat3; OCT3; Roct"
                     /note="34 a nucleotides"
ORIGIN      
        1 ctgagctgtc ctaccacagc agccgccgga ccctaggaca gagcacgggc caccgccgca
       61 tccacctcca gtccaactgg atccagctcc aaccaccagt tttggttcat cttgcctggt
      121 gccatgacct tctccgagat tctggaccgt gtcggaagca tgggcccctt ccagtacctg
      181 catgtgacct tgctggccct cccagtcctc ggaatagcca accacaactt gctacagatc
      241 ttcacagcca ccacccctgt ccaccactgt cgcccgcccc ccaacgcctc tatagggccc
      301 tgggtactcc ccttggaccc aaatgggaag cctgagaagt gtctccgctt cgtacatctg
      361 ccaaatgcca gtcttcccaa tgacacccag agggccaccg agccgtgctt ggatggctgg
      421 atctacaaca gcaccagaga caccattgtg atagagtggg acttggtgtg cagctccaac
      481 aaactgaagg agatggccca gtcgatcttc atggcaggca tactggttgg aggacctgtg
      541 attggagaac tgtcagacag gtttggccgc aagcctatcc tgacctggag ttatctcatg
      601 ctggcagcca gcggctctgg tgctgccttc agtcccagcc tccctgtcta tatgatcttc
      661 cgattcctgt gtggctgcag catctcgggc atttctctga gcaccgttat cttgaatgtg
      721 gaatgggtac ccacctcgat gcgggccatc tcatcaacat ctattgggta ctgctacacc
      781 attggtcagt tcattctgtc cggcctggcc tatgccattc ctcagtggcg ctggctacag
      841 ttaacctcgt ctgctccctt cttcatcttc tccttgttgt cctggtgggt accagagtcc
      901 atacgctggc tggttctatc tggaaaatac tcaaaggccc tgaagacact ccaacgggtg
      961 gctaccttca acggcaagaa ggaggaaggg aaaaagctca ccatagagga gctgaagttc
     1021 aacttgcaga aggacatcac ctcagccaag gtcaaatatg gcttatctga cttgttccgg
     1081 gtgtccatcc ttcgtcgtgt gaccttctgt ctctctctgg cctggttttc tactggtttt
     1141 gcctactaca gtttggctat gggggtagaa gaatttggag tcaacatcta catactccag
     1201 attatctttg gtggggttga catcccagcc aagttcatca caatcctctc cttaagttat
     1261 ctgggccggc gcatcactca gagcttcctc ctgctcctag caggaggggc cattttggcc
     1321 ctcatctttg tgccttcaga aatgcagctc ttgagaacag cactggctgt gtttggaaag
     1381 ggatgcctat ctggctcctt cagctgcctc ttcctctaca cgagtgagct ctaccctaca
     1441 gtcctcaggc aaacaggtat gggtatcagt aacgtgtggg ctcgagtagg aagtatgata
     1501 gccccactgg tgaaaatcac gggtgaactg cagcccttca tccctaatgt catctttggg
     1561 accacggccc tactgggagg cagtgctgcc ttctttctgc ttgagaccct caatcggccc
     1621 ttaccggaga ctatcgagga catacaaaac tggcacaagc aagtccagaa aacaaagcag
     1681 gagtcggaag cagaaaaggc atcccaaata atcccgctga agactggtgg ataggaccct
     1741 agctgagaac aacagaatcc tctttcctgg ccacaagaga ctgatcccaa gcagtaccct
     1801 tctggagttc cttgggcacc ttgggggttg gggaaagccc taggtgggcc catgctcttg
     1861 gaacaaaaac ttctgagagt tcagtaaagg tgttctaccc tcatcacctc caccatagcc
     1921 tacaacccag acccggcctg ctcacagctc tagccatagg cttcccatac tcctgcactc
     1981 atcctccctg cagcccagcc ctgccattct tctgtcaacc cttgccatat tggccatttc
     2041 ctccattgtc ccacctccat tttccttgag atcccctagc agttctaatg gtttcttctt
     2101 accttgccca aactctctcc ttggtgggaa atttcaataa accacaatga agaactc
//
