LOCUS       NM_032065               1990 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus solute carrier family 1 member 6 (Slc1a6), mRNA.
ACCESSION   NM_032065
VERSION     NM_032065.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abousaab,A., Warsi,J., Elvira,B. and Lang,F.
  TITLE     Caveolin-1 Sensitivity of Excitatory Amino Acid Transporters EAAT1,
            EAAT2, EAAT3, and EAAT4
  JOURNAL   J Membr Biol 249 (3), 239-249 (2016)
   PUBMED   26690923
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Dalet,A., Bonsacquet,J., Gaboyard-Niay,S., Calin-Jageman,I.,
            Chidavaenzi,R.L., Venteo,S., Desmadryl,G., Goldberg,J.M.,
            Lysakowski,A. and Chabbert,C.
  TITLE     Glutamate transporters EAAT4 and EAAT5 are expressed in vestibular
            hair cells and calyx endings
  JOURNAL   PLoS One 7 (9), e46261 (2012)
   PUBMED   23049999
REFERENCE   3  (bases 1 to 1990)
  AUTHORS   Machtens,J.P., Kovermann,P. and Fahlke,C.
  TITLE     Substrate-dependent gating of anion channels associated with
            excitatory amino acid transporter 4
  JOURNAL   J Biol Chem 286 (27), 23780-23788 (2011)
   PUBMED   21572047
  REMARK    GeneRIF: strate-dependent gating of anion channels associated with
            excitatory amino acid transporter 4.
REFERENCE   4  (bases 1 to 1990)
  AUTHORS   Nothmann,D., Leinenweber,A., Torres-Salazar,D., Kovermann,P.,
            Hotzy,J., Gameiro,A., Grewer,C. and Fahlke,C.
  TITLE     Hetero-oligomerization of neuronal glutamate transporters
  JOURNAL   J Biol Chem 286 (5), 3935-3943 (2011)
   PUBMED   21127051
  REMARK    GeneRIF: EAAT3 and EAAT4, but neither EAAT1 and EAAT2, nor EAAT2
            and EAAT3 are capable of co-assembling into heterotrimers.
            Co-expression of EAAT3 and EAAT4 results in additional appearance
            of basolateral EAAT3
REFERENCE   5  (bases 1 to 1990)
  AUTHORS   Satake,S., Song,S.Y., Konishi,S. and Imoto,K.
  TITLE     Glutamate transporter EAAT4 in Purkinje cells controls
            intersynaptic diffusion of climbing fiber transmitter mediating
            inhibition of GABA release from interneurons
  JOURNAL   Eur J Neurosci 32 (11), 1843-1853 (2010)
   PUBMED   21070388
  REMARK    GeneRIF: Data demonstrate that the neuronal glutamate transporter
            EAAT4 in Purkinje cells plays a critical role in the extrasynaptic
            diffusion of climbing fiber transmitters.
REFERENCE   6  (bases 1 to 1990)
  AUTHORS   Melzer,N., Biela,A. and Fahlke,C.
  TITLE     Glutamate modifies ion conduction and voltage-dependent gating of
            excitatory amino acid transporter-associated anion channels
  JOURNAL   J Biol Chem 278 (50), 50112-50119 (2003)
   PUBMED   14506254
  REMARK    GeneRIF: EAAT4 but not EAAT2 anion channels displays
            voltage-dependent gating modified by glutamate
REFERENCE   7  (bases 1 to 1990)
  AUTHORS   Cholet,N., Pellerin,L., Magistretti,P.J. and Hamel,E.
  TITLE     Similar perisynaptic glial localization for the Na+,K+-ATPase alpha
            2 subunit and the glutamate transporters GLAST and GLT-1 in the rat
            somatosensory cortex
  JOURNAL   Cereb Cortex 12 (5), 515-525 (2002)
   PUBMED   11950769
  REMARK    GeneRIF: The aspartate/glutamate transporter is localized in
            astrocytic processes around axonal or dendritic elements of
            glutamatergic axo-dendritic synaptic junctions in the
            frontoparietal somatosensory cortex.
REFERENCE   8  (bases 1 to 1990)
  AUTHORS   Dehnes,Y., Chaudhry,F.A., Ullensvang,K., Lehre,K.P.,
            Storm-Mathisen,J. and Danbolt,N.C.
  TITLE     The glutamate transporter EAAT4 in rat cerebellar Purkinje cells: a
            glutamate-gated chloride channel concentrated near the synapse in
            parts of the dendritic membrane facing astroglia
  JOURNAL   J Neurosci 18 (10), 3606-3619 (1998)
   PUBMED   9570792
REFERENCE   9  (bases 1 to 1990)
  AUTHORS   Furuta,A., Rothstein,J.D. and Martin,L.J.
  TITLE     Glutamate transporter protein subtypes are expressed differentially
            during rat CNS development
  JOURNAL   J Neurosci 17 (21), 8363-8375 (1997)
   PUBMED   9334410
REFERENCE   10 (bases 1 to 1990)
  AUTHORS   Fairman,W.A., Vandenberg,R.J., Arriza,J.L., Kavanaugh,M.P. and
            Amara,S.G.
  TITLE     An excitatory amino-acid transporter with properties of a
            ligand-gated chloride channel
  JOURNAL   Nature 375 (6532), 599-603 (1995)
   PUBMED   7791878
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Nov 27, 2020 this sequence version replaced NM_032065.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U89608.1, BC098912.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               JAXUCZ010000007.1  11520863-11521007   c
            146-348             JAXUCZ010000007.1  11513978-11514180   c
            349-486             JAXUCZ010000007.1  11511688-11511825   c
            487-691             JAXUCZ010000007.1  11509011-11509215   c
            692-734             JAXUCZ010000007.1  11507188-11507230   c
            735-1078            JAXUCZ010000007.1  11505326-11505669   c
            1079-1312           JAXUCZ010000007.1  11499354-11499587   c
            1313-1507           JAXUCZ010000007.1  11497614-11497808   c
            1508-1642           JAXUCZ010000007.1  11494170-11494304   c
            1643-1990           JAXUCZ010000007.1  11492422-11492769   c
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..1990
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /note="solute carrier family 1 member 6"
                     /db_xref="GeneID:84012"
                     /db_xref="RGD:620340"
     exon            1..145
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            146..348
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..1838
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /note="high affinity aspartate/glutamate transporter;
                     high-affinity neuronal glutamate transporter;
                     sodium-dependent glutamate/aspartate transporter; solute
                     carrier family 1 (high affinity aspartate/glutamate
                     transporter), member 6"
                     /codon_start=1
                     /product="excitatory amino acid transporter 4"
                     /protein_id="NP_114454.2"
                     /db_xref="GeneID:84012"
                     /db_xref="RGD:620340"
                     /translation="MSSHGNSLFLRESGAGGGCLQGLQDSLQQRALRTRLRLQTMTRE
                     HVRRFLRRNAFILLTVSAVIIGVSLAFALRPYQLSYRQIKYFSFPGELLMRMLQMLVL
                     PLIVSSLVTGMASLDNKATGRMGMRAAVYYMVTTVIAVFIGILMVTIIHPGKGSKEGL
                     HREGRIETVPTADAFMDLVRNMFPPNLVEACFKQFKTQYSTRVVTRTIVRTDNGSELG
                     ASISPPSSAENETSILENVTRALGTLQEVISFEETVPVPGSANGINALGLVVFSVAFG
                     LVIGGMKHKGRVLRDFFDSLNEAIMRLVGIIIWYAPVGILFLIAGKILEMEDMAVLGG
                     QLGMYTLTVIVGLFLHAGGVLPLIYFLVTHRNPFPFIGGILQALITAMGTSSSSATLP
                     ITFRCLEEGLGVDRRITRFVLPVGATVNMDGTALYEALAAIFIAQVNNYELNLGQITT
                     ISITATAASVGAAGIPQAGLVTMVIVLTSVGLPTEDITLIIAVDWFLDRLRTMTNVLG
                     DSIGAAVIEHLSQRELELQEAELTLPSLGKPYKSLMAQEKGASRGRGGNESVM"
     exon            349..486
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            487..691
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            692..734
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            735..1078
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1312
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1507
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1642
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1990
                     /gene="Slc1a6"
                     /gene_synonym="EAAT4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttcgggtccc caacgcagac acagagcggg tccctggcgg gtccctgcgg acccggccag
       61 gcgcttccgc gtgttctggc ttttgcatcc cggcgcagcg cgcaggcgga ggcgcaggca
      121 aggcggcccc gctgacccga ggctgagacg cgatgagcag ccacggcaac agtctgttcc
      181 tgagggagag cggcgctggc gggggctgtc tgcaaggcct gcaggacagt ctgcagcaga
      241 gagcactgcg cacgcgcttg cgcctgcaga ccatgacccg agagcacgtg cggcgcttcc
      301 tgcgccgaaa tgccttcatc ttgctcacag tcagcgctgt gatcattggc gtcagcctgg
      361 catttgcctt gcgcccatac cagctctcct accgccagat caagtacttc tcttttcctg
      421 gtgagctgct catgaggatg ctgcagatgc tggtgctacc cctcattgtc tccagccttg
      481 tcacaggtat ggcatccttg gacaacaagg caacagggag gatgggaatg agggcagctg
      541 tgtactacat ggtgacgacc gtcattgcag ttttcatcgg catcctaatg gttaccatca
      601 tacatcctgg gaagggctcc aaggaggggc tgcaccgtga gggccgaatt gagactgttc
      661 caacagctga tgctttcatg gacctggtca gaaatatgtt tccaccaaac cttgtggagg
      721 cctgcttcaa acagtttaaa acacagtaca gcacacgagt ggtaacaagg acgattgtaa
      781 ggacagacaa cgggtcagag ttgggggcct ccatttctcc tccatcctca gcagaaaatg
      841 aaaccagcat cctggaaaat gtcacccggg ccttgggcac cctgcaggag gtgataagct
      901 ttgaggagac tgtgcctgta cctggctcag ctaatggcat caacgctttg ggcctcgtgg
      961 tcttctctgt ggccttcggg ctggtcatcg gtggcatgaa gcacaaaggc cgtgtcctga
     1021 gggatttctt cgacagcctc aatgaggcta ttatgaggct ggtgggcatc atcatctggt
     1081 atgcaccagt gggcatcctg ttcctgattg ctggaaagat tctggaaatg gaagacatgg
     1141 ctgtccttgg aggtcagctg ggcatgtaca cgctgactgt cattgtcggc ttatttcttc
     1201 atgctggtgg tgtgttaccc ctcatctact tccttgtcac acatcggaac ccctttccct
     1261 tcatcggtgg tatactacag gcactcatca cagccatggg tacctcttcc agctctgcaa
     1321 ctctgcctat cactttccga tgcctggagg agggcctggg tgtggaccgc cgcatcacca
     1381 gattcgtgtt gcctgtgggg gccactgtca acatggacgg caccgcgctc tatgaggcct
     1441 tggcagccat ctttattgct caagtcaaca actatgagct gaaccttggt cagatcacca
     1501 caatcagcat cacagccaca gctgccagcg taggagcagc tggcatcccc caggcaggac
     1561 tggttaccat ggtgattgtg ctcacatccg tcggcctgcc cacagaggac atcacattga
     1621 tcatagctgt ggattggttc cttgatcgac ttcgtacgat gaccaatgta cttggggact
     1681 caattggagc agctgtcatt gagcatttgt cccaacggga gctggagctg caagaggctg
     1741 agctgactct acccagcctg gggaaaccct ataagtcact catggcacaa gaaaaggggg
     1801 catcaagggg tcggggaggt aatgagagtg tcatgtgaga agcttctagc tctctgtcca
     1861 gggcggaggg aaggggctgg gaggggagtc ctggggacaa tctgttgcac aactgactgt
     1921 gggctgcaca tacatgttct gtgtgggttc tctggggact gctgagataa aagagaagga
     1981 atgaaaggta
//
