LOCUS       NM_019225               3922 bp    mRNA    linear   ROD 27-JUL-2024
DEFINITION  Rattus norvegicus solute carrier family 1 member 3 (Slc1a3),
            transcript variant Glast, mRNA.
ACCESSION   NM_019225
VERSION     NM_019225.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Zhou,Z.X., Xu,L.J., Wang,H.N., Cheng,S., Li,F., Miao,Y., Lei,B.,
            Gao,F. and Wang,Z.
  TITLE     EphA4/ephrinA3 reverse signaling mediated downregulation of
            glutamate transporter GLAST in Muller cells in an experimental
            glaucoma model
  JOURNAL   Glia 71 (3), 720-741 (2023)
   PUBMED   36416239
  REMARK    GeneRIF: EphA4/ephrinA3 reverse signaling mediated downregulation
            of glutamate transporter GLAST in Muller cells in an experimental
            glaucoma model.
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Zhao,Y.Z., Wei,J., Song,K.X., Zhou,C. and Chai,Z.
  TITLE     Glutamate-aspartate transporter 1 attenuates oxygen-glucose
            deprivation-induced injury by promoting glutamate metabolism in
            primary cortical neurons
  JOURNAL   J Cell Physiol 237 (7), 3044-3056 (2022)
   PUBMED   35551669
  REMARK    GeneRIF: Glutamate-aspartate transporter 1 attenuates
            oxygen-glucose deprivation-induced injury by promoting glutamate
            metabolism in primary cortical neurons.
REFERENCE   3  (bases 1 to 3922)
  AUTHORS   Kostic,M., Zivkovic,N., Cvetanovic,A., Stojanovic,I. and Colic,M.
  TITLE     IL-17 signalling in astrocytes promotes glutamate excitotoxicity:
            Indications for the link between inflammatory and neurodegenerative
            events in multiple sclerosis
  JOURNAL   Mult Scler Relat Disord 11, 12-17 (2017)
   PUBMED   28104249
  REMARK    GeneRIF: Results suggest that, via astrocytes, IL-17A could promote
            glutamate excitotoxicity in multiple terms: by decreasing the
            astrocyte ability to uptake extracellular glutamate, due to a
            reduced gene expression of glutamate transporters (GLAST and
            GLT-1); by decreasing intracellular glutamate conversion into
            non-toxic glutamine; by potentiating glutamate exocytosis in a Ca2+
            dependent manner
REFERENCE   4  (bases 1 to 3922)
  AUTHORS   Krycer,J.R., Fazakerley,D.J., Cater,R.J., C Thomas,K., Naghiloo,S.,
            Burchfield,J.G., Humphrey,S.J., Vandenberg,R.J., Ryan,R.M. and
            James,D.E.
  TITLE     The amino acid transporter, SLC1A3, is plasma membrane-localised in
            adipocytes and its activity is insensitive to insulin
  JOURNAL   FEBS Lett 591 (2), 322-330 (2017)
   PUBMED   28032905
REFERENCE   5  (bases 1 to 3922)
  AUTHORS   Lee,A., Anderson,A.R., Beasley,S.J., Barnett,N.L., Poronnik,P. and
            Pow,D.V.
  TITLE     A new splice variant of the glutamate-aspartate transporter:
            cloning and immunolocalization of GLAST1c in rat, pig and human
            brains
  JOURNAL   J Chem Neuroanat 43 (1), 52-63 (2012)
   PUBMED   22026960
REFERENCE   6  (bases 1 to 3922)
  AUTHORS   Huggett,J., Vaughan-Thomas,A. and Mason,D.
  TITLE     The open reading frame of the Na(+)-dependent glutamate transporter
            GLAST-1 is expressed in bone and a splice variant of this molecule
            is expressed in bone and brain
  JOURNAL   FEBS Lett 485 (1), 13-18 (2000)
   PUBMED   11086157
REFERENCE   7  (bases 1 to 3922)
  AUTHORS   Inoue,K., Sakaitani,M., Shimada,S. and Tohyama,M.
  TITLE     Cloning and expression of a bovine glutamate transporter
  JOURNAL   Brain Res Mol Brain Res 28 (2), 343-348 (1995)
   PUBMED   7723632
REFERENCE   8  (bases 1 to 3922)
  AUTHORS   Arriza,J.L., Fairman,W.A., Wadiche,J.I., Murdoch,G.H.,
            Kavanaugh,M.P. and Amara,S.G.
  TITLE     Functional comparisons of three glutamate transporter subtypes
            cloned from human motor cortex
  JOURNAL   J Neurosci 14 (9), 5559-5569 (1994)
   PUBMED   7521911
REFERENCE   9  (bases 1 to 3922)
  AUTHORS   Li,H.S., Niedzielski,A.S., Beisel,K.W., Hiel,H., Wenthold,R.J. and
            Morley,B.J.
  TITLE     Identification of a glutamate/aspartate transporter in the rat
            cochlea
  JOURNAL   Hear Res 78 (2), 235-242 (1994)
   PUBMED   7527019
REFERENCE   10 (bases 1 to 3922)
  AUTHORS   Storck,T., Schulte,S., Hofmann,K. and Stoffel,W.
  TITLE     Structure, expression, and functional analysis of a Na(+)-dependent
            glutamate/aspartate transporter from rat brain
  JOURNAL   Proc Natl Acad Sci U S A 89 (22), 10955-10959 (1992)
   PUBMED   1279699
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            FQ097869.1, AF265360.1, FM094687.1 and S59158.1.
            
            On Feb 11, 2014 this sequence version replaced NM_019225.1.
            
            Transcript Variant: This variant (Glast) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760393,
                              SAMEA5760396 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-770               FQ097869.1         1-770
            771-2207            AF265360.1         493-1929
            2208-2516           FM094687.1         349-657
            2517-3922           S59158.1           2459-3864
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q16"
     gene            1..3922
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /note="solute carrier family 1 member 3"
                     /db_xref="GeneID:29483"
                     /db_xref="RGD:3698"
     exon            1..164
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            165..442
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             262..1893
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /note="isoform 1 is encoded by transcript variant Glast;
                     glutamate transporter; glutamate/aspartate transporter;
                     excitatory amino acid transporter 1; solute carrier family
                     1 (glial high affinity glutamate transporter), member 3;
                     glial glutamate transporter; sodium-dependent
                     glutamate/aspartate transporter 1"
                     /codon_start=1
                     /product="excitatory amino acid transporter 1 isoform 1"
                     /protein_id="NP_062098.1"
                     /db_xref="GeneID:29483"
                     /db_xref="RGD:3698"
                     /translation="MTKSNGEEPRMGSRMERFQQGVRKRTLLAKKKVQNITKEDVKSY
                     LFRNAFVLLTVSAVIVGTILGFALRPYKMSYREVKYFSFPGELLMRMLQMLVLPLIIS
                     SLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGIIIVIIIHPGKGTKENMYREGK
                     IVQVTAADAFLDLIRNMFPPNLVEACFKQFKTSYEKRSFKVPIQANETLLGAVINNVS
                     EAMETLTRIREEMVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNE
                     AIMRLVAVIMWYAPLGILFLIAGKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLP
                     LLYFLVTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRITRFVLP
                     VGATINMDGTALYEALAAIFIAQVNNFDLNFGQIITISITATAASIGAAGIPQAGLVT
                     MVIVLTSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVE
                     MGNSVIEENEMKKPYQLIAQDNEPEKPVADSETKM"
     exon            443..580
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            581..785
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            786..828
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            829..1121
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..1355
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1550
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1685
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..3914
                     /gene="Slc1a3"
                     /gene_synonym="EAAT1; GLAST; GLAST-1; GluT-1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aagttgcctg atagtaagtg gcggtttcgg agcacatgca tgttgtcagg gccagcctgc
       61 ccctccgctc gccgctcgct gagctgttac tccgagctac ctgctgggga aattcacttc
      121 gacgtagcct ggggtgtctt ccaccagtca cagaatcaga aaagttgtcc tctctaacac
      181 caaagaggag atttcgcttt ctggggacaa gttcaagaca ctgaagtgca aggctgtggt
      241 aaattcctgg aaagataaaa tatgacaaaa agcaacggag aagagcccag gatgggaagc
      301 aggatggaaa gattccagca aggggtgcgc aagcggacgc tcctggccaa gaagaaagtt
      361 cagaacatca ccaaggagga tgtgaagagc tacctgtttc ggaatgcctt tgtgctactc
      421 accgtcagcg ctgtcattgt gggtacaatc cttggatttg ccctccgacc gtataaaatg
      481 agctaccggg aggtcaagta cttctccttt cctggggagc ttctgatgcg gatgctgcag
      541 atgttggtct tacccctgat catctccagt cttgtcacag gaatggcggc cctagatagt
      601 aaggcatctg ggaagatggg gatgcgagct gtggtctatt acatgactac caccatcatt
      661 gctgtggtga tcggcataat cattgtcatc atcatccacc ccggaaaggg cacgaaagaa
      721 aacatgtaca gagaaggtaa aatcgtgcag gtgactgccg cagatgcctt cctggattta
      781 atcaggaaca tgttcccacc caatctggta gaagcctgct ttaaacagtt taaaaccagc
      841 tatgagaaga gaagctttaa agtgcccatc caggccaacg aaacactgtt gggcgccgtg
      901 atcaacaacg tgtcagaggc catggagact ctgacccgga tccgggagga gatggtaccc
      961 gttcctgggt ctgtgaatgg ggtcaatgcc ctgggcctcg ttgtcttctc catgtgcttc
     1021 ggcttcgtga tcggaaacat gaaggagcag gggcaagcgc taagagagtt cttcgactct
     1081 ctcaatgaag ccatcatgag attggtagcg gtgataatgt ggtatgcacc tctgggcatc
     1141 ctcttcttga tcgcagggaa gattgttgag atggaagata tgggtgtgat tggggggcag
     1201 cttgccatgt atacagtgac agtcatcgtc ggcctcctca ttcatgccgt catcgtcctg
     1261 cctctcctct acttcctggt aacccggaag aacccctggg ttttcattgg agggttgctg
     1321 caagcactca tcacagccct ggggacctcc tcaagttctg ccaccctgcc catcactttc
     1381 aagtgcctgg aagaaaacaa tggtgtggac aaacgcatca ccagatttgt gcttcccgtg
     1441 ggggccacca ttaacatgga tgggaccgcc ctctacgagg ctttggccgc cattttcatc
     1501 gctcaagtta acaactttga cctgaatttt ggacagatta taacaataag tatcacagcc
     1561 acagccgcaa gcattggggc agctggcatt cctcaggccg gtctagtcac catggtcatc
     1621 gtgctgacat ctgtgggcct gcccacggat gacatcacac tcatcattgc agtggactgg
     1681 tttctggacc gcctccgaac caccaccaac gtgctgggtg actccctcgg ggccgggatt
     1741 gttgaacact tgtcccgaca tgaactgaag aaccgagatg ttgaaatggg gaactccgtg
     1801 attgaggaga atgaaatgaa aaagccgtat cagctgattg cccaggacaa tgaaccagag
     1861 aaacccgtgg cagacagcga aaccaagatg tagactaaca cagaagtgct ttcttaagca
     1921 ccaggtgttg gaaactgttc tacaatgtgt ccatctccca gagctctctc tcccagtgag
     1981 ctcctctttc ctccctactc tgataggatt ggaaaatgtc caaaaacaaa ggagggctct
     2041 gcagcagcca aaacgtattg gttttagccc tcatttgaaa attttaaatc atttcgtatt
     2101 attcttacca agtaagttac tacaaacatt accaatttag atgacaaatg atcccttgtg
     2161 attgttttgt aagtaaaagc attaagcaaa tgataggcta caaaaacgtt ttaaaaccaa
     2221 ctttcaaaat gtaaaaatcc ttcagaacac tactgagttt tagtcttaaa acatagcaac
     2281 ttgatgagca attatcagtt acccgttggg tagctgagag gtttgccctt tcttctgact
     2341 ctcatcctga ctttatcagc atcagggcaa tgcccacatg cacacagcac agccgtgagg
     2401 tggagcagaa agtgaggctc cccaatggtc ttgagtgagc cttgtctcat ccctgggcct
     2461 cagtgttctc atgcagaaaa tgagtgaccc ctagatacca taggcctctc tagcccagac
     2521 ttttacaaca tcaggaagga ccctgcccct gtataacatg aggatttcag acaggacact
     2581 taatgggagt ggacatttct ctctaggggc aggctgcgtg tggctcacta gatcctgggt
     2641 ccagaatgtg ggttatataa gtagacaggt ggaaggccat cccgggagac catccccttg
     2701 gcctgttgtt ctgttttatc ttgttttgtt ttccaacatt aaaagaagca ctcccttctt
     2761 gggtactctt cccaaaccca agggaagaaa gcctcccacc acctcttcga ctgacagtga
     2821 gatgtttagg aaacaaggag aagactagca gctagcaagt gtgtccatgg tcttactacg
     2881 gaacacaaaa gagcagaggc ggaatagagt acagcccaca ttacaaagga ggattctttt
     2941 taagatcaga aaaagttggt tttctttaaa gactaaaaga gagctgaacc ttggccttct
     3001 tctgctggcc taaacgcttt gtcttctgtt cctaatcaat ccccaaagaa acaacacata
     3061 acatcactcc tttcttattt cacagtccag caaatgatat ctctggaaca gattcatatc
     3121 tataagctgg gtggatggtc tcatttccaa aaaaaaaagt ttaaaatttg gttagttttt
     3181 cctattttaa ctggaagttc ttaggatgac aaaaccacat actcctttgt acagagatgt
     3241 ctcttgacaa atcattcaag actctcatct agaatggagt ttgcttgtgt ccctgtctcc
     3301 ccctccctaa atgcagatga atcagcatga aaataacatc aacgagggtg gaagaaaaga
     3361 gggcaaagga attgttttca ggcgaggcag ctcccaggtt gaaaagagat ttttttactt
     3421 ccttaggaat atccaatcaa agatactcaa tgggagccct ctgacagtac cagctgtgtt
     3481 tctttctgag agaaaatgct gagtggacac cattatgcta acagactgaa ggtctagact
     3541 ccttgtgtct cccgatctac tgtagagata ctttctagag attctgagcg ttacatgcgt
     3601 ataaaggtta acactaggct acagtggaat agtcatttaa tggcaggctc aatttgggaa
     3661 gtacactaca gaggttaacc tatgtggcag tcgttttgcg acgctagtag aacaaactta
     3721 accgcgtcaa attaacgctg tttacccagt gtgatcatat tttttatact aaccgtttct
     3781 tacgaaaact ggtcctctag ggtgatcctt tagccagccc atcacaggct gcgcagatgt
     3841 gtgtacccac tgtggactgg gaaacatcac tttgtagatg tattttcaat aaagaaaaga
     3901 tagttttaca tgaaaaaaaa aa
//
