LOCUS       NM_017299               2436 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus solute carrier family 19 member 1 (Slc19a1),
            transcript variant 1, mRNA.
ACCESSION   NM_017299 XM_346600
VERSION     NM_017299.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Zhang,S.S., Wu,Y.J., Pan,S., Wang,Q.H., Chen,W.G., Wang,Z.F.,
            Xu,J.Y., Zuo,J. and Yin,Q.
  TITLE     Xanthones from securidaca inappendiculata antagonizes the
            anti-rheumatic effect of methotrexate by inhibiting reduced folate
            carrier 1
  JOURNAL   Immunopharmacol Immunotoxicol 45 (1), 16-25 (2023)
   PUBMED   35850595
  REMARK    GeneRIF: Xanthones from securidaca inappendiculata antagonizes the
            anti-rheumatic effect of methotrexate by inhibiting reduced folate
            carrier 1.
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Radziejewska,A., Suliburska,J., Kolodziejski,P. and Chmurzynska,A.
  TITLE     Role of Slc19a1 and Tfr2 in liver transport of iron and folate: A
            rat model of folate/iron deficiency followed by supplementation
  JOURNAL   J Trace Elem Med Biol 62, 126568 (2020)
   PUBMED   32521439
  REMARK    GeneRIF: Role of Slc19a1 and Tfr2 in liver transport of iron and
            folate: A rat model of folate/iron deficiency followed by
            supplementation.
REFERENCE   3  (bases 1 to 2436)
  AUTHORS   Luteijn,R.D., Zaver,S.A., Gowen,B.G., Wyman,S.K., Garelis,N.E.,
            Onia,L., McWhirter,S.M., Katibah,G.E., Corn,J.E., Woodward,J.J. and
            Raulet,D.H.
  TITLE     SLC19A1 transports immunoreactive cyclic dinucleotides
  JOURNAL   Nature 573 (7774), 434-438 (2019)
   PUBMED   31511694
  REMARK    Erratum:[Nature. 2020 Mar;579(7800):E12. doi:
            10.1038/s41586-020-2064-8.. PMID: 32144410]
REFERENCE   4  (bases 1 to 2436)
  AUTHORS   Ritchie,C., Cordova,A.F., Hess,G.T., Bassik,M.C. and Li,L.
  TITLE     SLC19A1 Is an Importer of the Immunotransmitter cGAMP
  JOURNAL   Mol Cell 75 (2), 372-381 (2019)
   PUBMED   31126740
REFERENCE   5  (bases 1 to 2436)
  AUTHORS   Alam,C., Hoque,M.T., Finnell,R.H., Goldman,I.D. and Bendayan,R.
  TITLE     Regulation of Reduced Folate Carrier (RFC) by Vitamin D Receptor at
            the Blood-Brain Barrier
  JOURNAL   Mol Pharm 14 (11), 3848-3858 (2017)
   PUBMED   28885847
REFERENCE   6  (bases 1 to 2436)
  AUTHORS   Rajgopal,A., Sierra,E.E., Zhao,R. and Goldman,I.D.
  TITLE     Expression of the reduced folate carrier SLC19A1 in IEC-6 cells
            results in two distinct transport activities
  JOURNAL   Am J Physiol Cell Physiol 281 (5), C1579-C1586 (2001)
   PUBMED   11600421
REFERENCE   7  (bases 1 to 2436)
  AUTHORS   Chancy,C.D., Kekuda,R., Huang,W., Prasad,P.D., Kuhnel,J.M.,
            Sirotnak,F.M., Roon,P., Ganapathy,V. and Smith,S.B.
  TITLE     Expression and differential polarization of the reduced-folate
            transporter-1 and the folate receptor alpha in mammalian retinal
            pigment epithelium
  JOURNAL   J Biol Chem 275 (27), 20676-20684 (2000)
   PUBMED   10787414
REFERENCE   8  (bases 1 to 2436)
  AUTHORS   Honscha,W., Dotsch,K.U., Thomsen,N. and Petzinger,E.
  TITLE     Cloning and functional characterization of the bile acid-sensitive
            methotrexate carrier from rat liver cells
  JOURNAL   Hepatology 31 (6), 1296-1304 (2000)
   PUBMED   10827155
REFERENCE   9  (bases 1 to 2436)
  AUTHORS   Tse,A., Brigle,K., Taylor,S.M. and Moran,R.G.
  TITLE     Mutations in the reduced folate carrier gene which confer dominant
            resistance to 5,10-dideazatetrahydrofolate
  JOURNAL   J Biol Chem 273 (40), 25953-25960 (1998)
   PUBMED   9748272
REFERENCE   10 (bases 1 to 2436)
  AUTHORS   Chiao,J.H., Roy,K., Tolner,B., Yang,C.H. and Sirotnak,F.M.
  TITLE     RFC-1 gene expression regulates folate absorption in mouse small
            intestine
  JOURNAL   J Biol Chem 272 (17), 11165-11170 (1997)
   PUBMED   9111015
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF099009.1 and BC085742.1.
            
            On Mar 31, 2009 this sequence version replaced NM_017299.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF099009.1, U38180.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16676806 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1983              AF099009.1         10-1992
            1984-2436           BC085742.1         1851-2303
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="20"
                     /map="20p12"
     gene            1..2436
                     /gene="Slc19a1"
                     /gene_synonym="MTX-1; MTX1"
                     /note="solute carrier family 19 member 1"
                     /db_xref="GeneID:29723"
                     /db_xref="RGD:3695"
     exon            1..201
                     /gene="Slc19a1"
                     /gene_synonym="MTX-1; MTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            202..431
                     /gene="Slc19a1"
                     /gene_synonym="MTX-1; MTX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             249..1787
                     /gene="Slc19a1"
                     /gene_synonym="MTX-1; MTX1"
                     /note="methotrexate carrier 1; folate transporter 1;
                     methotrexate carrier 6; methotrexate carrier 5;
                     methotrexate carrier 2; RFC-1; reduced folate carrier 1;
                     RFC1; solute carrier family 19 (folate transporter),
                     member 1; solute carrier family 19 (sodium/hydrogen
                     exchanger), member 1; plasma membrane folate antiporter
                     SLC19A1"
                     /codon_start=1
                     /product="reduced folate transporter"
                     /protein_id="NP_058995.2"
                     /db_xref="GeneID:29723"
                     /db_xref="RGD:3695"
                     /translation="MVPTGQVAEKQACEEPRQDRELKSWRWLVFYLCFFGFMAQLRPG
                     ESFITPYLLERNFTKEQVTNEIIPMLPYSHLAVLVPIFLLTDYLRYKPVLVLQCLSFV
                     CVWLLLLLGTSVVHMQLMEVFYSITMAARIAYSSYIFSLVQPSRYQRMASYSRAAVLL
                     GVFISSVLGQVLVTLGGISTYMLNCISLGFILFSLSLSLFLKRPKRSLFFNRSALVQG
                     ALPCELDQMHPGPGRPEPRKLERMLGTCRDSFLVRMLSELVKNVRQPQLRLWCLWWVF
                     NSAGYYLITYYVHVLWKITDSRLNYNGAVDAASTLLSAITAFTAGFVNIRWALWSKLV
                     IASVIAIQAGLVFCMFQIPDIWVCYVTFVLFRGAYQFLVPIATFQIASSLSKELCALV
                     FGINTFLATALKTSITLVVSDKRGLGLQVHQQFRIYFMYFLTLSIICLAWAGLDGLRY
                     YRRGRHQPLAQAQALSPLEDSVQAISLQDGDLRRPQPSAPQLLPEDGSVEDGRADLRV
                     EAKA"
     exon            432..1185
                     /gene="Slc19a1"
                     /gene_synonym="MTX-1; MTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..1378
                     /gene="Slc19a1"
                     /gene_synonym="MTX-1; MTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1520
                     /gene="Slc19a1"
                     /gene_synonym="MTX-1; MTX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..2411
                     /gene="Slc19a1"
                     /gene_synonym="MTX-1; MTX1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aacgccggct gctggttgaa ctaggtacgg gcctgtggga cgcagagctg gggatccatt
       61 ctgagtgcct agggagccac aaggtgcggt cctgttcctt ccggcatcct gaagatgaaa
      121 gcggactgaa ttctatactg cagtccctgg cgcaactgca gagctccggg cagggtttgc
      181 agcgtgccaa gagcctgcga agtggagtgt catctttgtt caccaggtcc catgaggacc
      241 tgggcaacat ggtgcccact ggccaggtgg cagaaaagca agcctgcgaa gagcccaggc
      301 aggaccgaga acttaagtcc tggcgatggc tcgtgttcta cctctgcttc tttggcttca
      361 tggcacagtt gcgacctggg gagagcttca tcacacccta cctcctggaa cgtaacttca
      421 ccaaggagca ggtgaccaac gagatcattc cgatgctgcc ctactcccac ctggccgtgc
      481 tggtccccat cttcctgctc accgactacc tacgatacaa gccagtcctg gtcctacagt
      541 gcctgagctt cgtgtgcgtg tggctgctgc tgctcctggg gacctccgtc gtgcacatgc
      601 agctcatgga agtcttctac agcatcacca tggcggcgcg catcgcctac tcctcctaca
      661 tattctccct ggtgcagccc tcccgctacc agcgcatggc cagctactcg cgggccgcag
      721 tcctactggg cgtcttcatc agctccgtgc tgggccaggt gctggtcacc ctaggaggca
      781 tctccaccta tatgctcaac tgtatctccc tgggcttcat cctcttcagc ctgagcctct
      841 ccctcttcct aaagcgtcct aagcggagcc tctttttcaa ccggagcgca cttgtgcaag
      901 gagccttgcc ctgtgagctg gatcagatgc acccgggacc tggccgtcca gagcccagga
      961 aactggagcg catgctgggc acttgcaggg actccttcct ggtacgcatg ctaagcgaac
     1021 tggtgaagaa cgtccggcaa ccacagctgc gtctctggtg cctctggtgg gtcttcaact
     1081 cggcaggcta ctacctgatc acctactatg tccatgtcct gtggaaaatc actgacagca
     1141 gactcaacta caacggtgcc gtagatgccg cctccacact tctgagcgcc atcacggcct
     1201 tcaccgcggg cttcgtgaac atccgctggg ctctgtggtc aaagctggtc atcgcgagtg
     1261 tcatagccat ccaggctggc ctggttttct gtatgttcca gatcccggac atctgggtct
     1321 gctatgtgac cttcgtgctt ttccgtgggg cctaccagtt ccttgtgccc attgccactt
     1381 ttcagattgc atcttccctg tctaaagagc tctgtgcatt ggtcttcggg atcaacactt
     1441 tccttgccac tgcgcttaag acctcgatca cactcgtggt ctctgacaag cggggtctgg
     1501 gcctccaagt ccatcaacag ttccgcatct acttcatgta cttcctgacg ctgtccatca
     1561 tctgccttgc ctgggctggg ctggacggct tgaggtacta ccggcgtggt cgccaccagc
     1621 cgctggccca ggcccaggct ctgagtccct tggaggattc tgtgcaggcg ataagcctac
     1681 aggatggaga cctaaggaga ccgcagccct cggcaccaca gctgctccca gaggatggca
     1741 gcgtagaaga cggcagggca gacttgagag tcgaagccaa ggcctgacct gtcttcacct
     1801 tcctggcttc atcccagttc agccgccgga ttcctgactc agtggcagcc cctcctgtgt
     1861 gcactccagc ctgaatcagc ccttctgggg tcccagtgcc tgagacatgt gggggccccc
     1921 ctgttgcctt tcaggagcct tcaggttagg ccacagaact ctctccttag gggccacctt
     1981 tttaaacagt ggggcgacag acagcttgtc cttccggtag cctctgagga ccctaagccc
     2041 agcctacttc atgctttagc aatggctgga gcctgctaca gacactcagg accgctccct
     2101 ggcttttgtg aaccacaatg tcaggccact acgagcctgg gttgcctact gcttgccaag
     2161 aagaacctgt gtaggggtca gctgtgccca ccaggctggt ctgccctttg ctctgggatg
     2221 ctcaggacaa atcacgtctg cccccagggc tagtgaggta gcccagacac cggacaaggt
     2281 ccgctgagcc tgcagggtac tggttacctg tcctaggctc ctggtgaagc tgccatacct
     2341 ccactctcgg aggtgcttgt ttgaatggtt gtctgatttt ttaaaacttg acattaaaat
     2401 ggagaactac aaaaaaaaaa aaaaaaaaaa aaaaaa
//
